Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.1.1.145 extracted from

  • Furster, C.; Zhang, J.; Toll, A.
    Purification of a 3beta-hydroxy-D5-C27-steroid dehydrogenase from pig liver microsomes active in major and alternative pathways of bile acid biosynthesis (1996), J. Biol. Chem., 271, 20903-20907.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.003
-
7alpha,27-dihydroxycholesterol
-
Sus scrofa
0.0032
-
7alpha-hydroxycholesterol
-
Sus scrofa

Localization

Localization Comment Organism GeneOntology No. Textmining
microsome
-
Sus scrofa
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
36000
-
x * 36000, SDS-PAGE Sus scrofa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
pregnenolone + NAD(P)+ Sus scrofa
-
pregn-5-en-3,20-dione + NAD(P)H + H+
-
?

Organism

Organism UniProt Comment Textmining
Sus scrofa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Sus scrofa

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Sus scrofa
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0033
-
7alpha,25-dihydroxycholesterol as substrate Sus scrofa
0.175
-
3beta,7alpha-dihydroxy-5-cholestenoic acid as substrate Sus scrofa
0.838
-
7alpha,27-dihydroxycholesterol as substrate Sus scrofa
1.489
-
7alpha-hydroxycholesterol as substrate Sus scrofa
2.88
-
-
Sus scrofa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3beta,17alpha-dihydroxy-5-pregnen-20-one + NAD(P)+
-
Sus scrofa 17alpha-hydroxypregn-5-ene-3,20-dione + NAD(P)H
-
?
3beta,7alpha-dihydroxy-5-cholestenoic acid + NAD+
-
Sus scrofa 7alpha-hydroxy-3-oxo-5-cholestenoic acid + NADH
-
?
7alpha,25-dihydroxycholesterol + NAD+
-
Sus scrofa 7alpha,25-dihydroxy-4-cholesten-3-one + NADH
-
?
7alpha,27-dihydroxycholesterol + NAD+
-
Sus scrofa 7alpha,27-dihydroxy-4-cholesten-3-one + NADH
-
?
7alpha-hydroxycholesterol + NAD+
-
Sus scrofa 7alpha-hydroxy-4-cholesten-3-one + NADH
-
?
pregnenolone + NAD(P)+
-
Sus scrofa pregn-5-en-3,20-dione + NAD(P)H + H+
-
?

Subunits

Subunits Comment Organism
? x * 36000, SDS-PAGE Sus scrofa

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Sus scrofa