Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | dehydrogenase is induced by limonene, alpha-pinene, delta-p-menthene and to lesser extend by p-cymene | Pseudomonas sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ag+ | 0.005 mM, complete inhibition, prevented by 10 mM glutathione | Pseudomonas sp. | |
Cu2+ | 5 mM, complete inhibition, prevented by 5 mM glutathione | Pseudomonas sp. | |
Hg2+ | 0.005 mM, complete inhibition, prevented by 10 mM glutathione | Pseudomonas sp. | |
iodoacetate | 10 mM, 95% inhibition, prevented by 10 mM glutathione | Pseudomonas sp. | |
additional information | glucose represses the induction of the enzyme by limonene | Pseudomonas sp. | |
NADH | competitive inhibition | Pseudomonas sp. | |
Ni2+ | 2 mM, 75% inhibition, complete reversion with 5 mM EDTA | Pseudomonas sp. | |
o-phenanthroline | at concentrations higher than 3 mM and low concentration of NAD+, about 50% inhibition | Pseudomonas sp. | |
p-hydroxymercuribenzoate | 0.005 mM, complete inhibition, prevented by 10 mM glutathione | Pseudomonas sp. | |
Zn2+ | 2 mM, 75% inhibition, complete reversion with 10 mM EDTA | Pseudomonas sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Pseudomonas sp. | |
0.077 | - |
NAD+ | at pH 8.6 | Pseudomonas sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
perillyl alcohol + NAD+ | Pseudomonas sp. | catalyzes dehydrogenation reaction which is a part of the catabolic sequence of alpha-pinenes and beta-pinenes, limonene, DELTA1-p-methene and p-cymene | perillyl aldehyde + NADH | - |
? | |
perillyl alcohol + NAD+ | Pseudomonas sp. PL- | catalyzes dehydrogenation reaction which is a part of the catabolic sequence of alpha-pinenes and beta-pinenes, limonene, DELTA1-p-methene and p-cymene | perillyl aldehyde + NADH | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas sp. | - |
PL-strain | - |
Pseudomonas sp. PL- | - |
PL-strain | - |
Purification (Comment) | Organism |
---|---|
PL-strain | Pseudomonas sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
13 | - |
DEAE-eluate | Pseudomonas sp. |
Storage Stability | Organism |
---|---|
-20°C, for at least 3 days, DEAE eluate | Pseudomonas sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | no substrates are p-menthan-7-ol, DELTA1-p-menthen-10-ol, DELTA8-menthen-10-ol, p-menthan-10-ol, myrtenol, isoborneol, borneol, cyclohexenol, o-menthyl benzyl alcohol, phthalyl alcohol, salicylic alcohol, 7-methyl cumic alcohol, ethanol, methanol | Pseudomonas sp. | ? | - |
? | |
additional information | no substrates are p-menthan-7-ol, DELTA1-p-menthen-10-ol, DELTA8-menthen-10-ol, p-menthan-10-ol, myrtenol, isoborneol, borneol, cyclohexenol, o-menthyl benzyl alcohol, phthalyl alcohol, salicylic alcohol, 7-methyl cumic alcohol, ethanol, methanol | Pseudomonas sp. PL- | ? | - |
? | |
perillyl alcohol + NAD+ | e.g.: 8-hydroxyphellandrol, cumic alcohol | Pseudomonas sp. | perillyl aldehyde + NADH | - |
r | |
perillyl alcohol + NAD+ | p-ethyl benzyl alcohol, p-methyl benzyl alcohol | Pseudomonas sp. | perillyl aldehyde + NADH | - |
r | |
perillyl alcohol + NAD+ | rate of the reverse reaction is about 20% of the rate of the forward reaction, oxidizes a number of primary alcohols with the alcohol group allylic to an endocyclic double bond and a 6-membered ring, either aromatic or hydroaromatic | Pseudomonas sp. | perillyl aldehyde + NADH | - |
r | |
perillyl alcohol + NAD+ | catalyzes dehydrogenation reaction which is a part of the catabolic sequence of alpha-pinenes and beta-pinenes, limonene, DELTA1-p-methene and p-cymene | Pseudomonas sp. | perillyl aldehyde + NADH | - |
? | |
perillyl alcohol + NAD+ | e.g.: 8-hydroxyphellandrol, cumic alcohol | Pseudomonas sp. PL- | perillyl aldehyde + NADH | - |
r | |
perillyl alcohol + NAD+ | p-ethyl benzyl alcohol, p-methyl benzyl alcohol | Pseudomonas sp. PL- | perillyl aldehyde + NADH | - |
r | |
perillyl alcohol + NAD+ | rate of the reverse reaction is about 20% of the rate of the forward reaction, oxidizes a number of primary alcohols with the alcohol group allylic to an endocyclic double bond and a 6-membered ring, either aromatic or hydroaromatic | Pseudomonas sp. PL- | perillyl aldehyde + NADH | - |
r | |
perillyl alcohol + NAD+ | catalyzes dehydrogenation reaction which is a part of the catabolic sequence of alpha-pinenes and beta-pinenes, limonene, DELTA1-p-methene and p-cymene | Pseudomonas sp. PL- | perillyl aldehyde + NADH | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
NADH + perillyl aldehyde | Pseudomonas sp. |
9.4 | - |
NAD+ + perillyl alcohol | Pseudomonas sp. |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 8 | pH 5: about 35% of activity maximum, pH 8: about 70% of activity maximum, NADH + perillyl aldehyde | Pseudomonas sp. |
6.3 | 10.5 | at pH 6.3 and 10.5: about 10% of activity maximum, perillyl alcohol + NAD+ | Pseudomonas sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | inactive with NADP+ and with thionicotinamide adenine dinucleotide | Pseudomonas sp. | |
NAD+ | also active with some NAD+ analogues, e.g.: 3-acetylpyridine adenine dinucleotide, 3-pyridinealdehyde adenine dinucleotide, deamino-NAD | Pseudomonas sp. | |
NADH | - |
Pseudomonas sp. |