Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | a 42- to 55-fold induction of the PGDH activity is observed after 20 days of gestation in pregnant rabbits | Oryctolagus cuniculus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
4-chloromercuriphenylsulfonic acid | - |
Oryctolagus cuniculus | |
N-ethylmaleimide | - |
Oryctolagus cuniculus | |
p-chloromercuriphenylsulfonic acid | - |
Oryctolagus cuniculus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.033 | - |
prostaglandin E1 | - |
Oryctolagus cuniculus | |
0.033 | - |
prostaglandin E1 | pH 10.2 | Oryctolagus cuniculus | |
0.038 | - |
prostaglandin A1 | - |
Oryctolagus cuniculus | |
0.059 | - |
prostaglandin E2 | - |
Oryctolagus cuniculus | |
0.059 | - |
prostaglandin E2 | pH 10.2 | Oryctolagus cuniculus | |
0.066 | - |
6-ketoprostaglandin E1 | - |
Oryctolagus cuniculus | |
0.111 | - |
6-ketoprostaglandin F1alpha | - |
Oryctolagus cuniculus | |
0.133 | - |
prostaglandin F2alpha | - |
Oryctolagus cuniculus | |
0.741 | - |
Prostaglandin D2 | - |
Oryctolagus cuniculus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Oryctolagus cuniculus | 5829 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
29000 | - |
x * 29000, SDS-PAGE | Oryctolagus cuniculus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(5Z,13E)-(15S)-11alpha,15-dihydroxy-9-oxoprost-5,13-dienoate + NAD+ | Oryctolagus cuniculus | first step in prostaglandin catabolism | (5Z,13E)-11alpha-hydroxy-9,15-dioxoprost-5,13-dienoate + NADH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Oryctolagus cuniculus | - |
- |
- |
Oryctolagus cuniculus | - |
pregnant rabbits | - |
Purification (Comment) | Organism |
---|---|
- |
Oryctolagus cuniculus |
native enzyme 2600fold from lung cytosol of pregnant rabbits, by cation and anion exchange chromatography, gel filtration, hydrophobic interaction and hydroxylapatite chromatography to homogeneity | Oryctolagus cuniculus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
lung | - |
Oryctolagus cuniculus | - |
lung | of 28-days-old pregnant rabbits | Oryctolagus cuniculus | - |
additional information | a 42- to 55-fold induction of the PGDH activity is observed after 20 days of gestation in pregnant rabbits | Oryctolagus cuniculus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
activity during pregnancy, substrate specificity | Oryctolagus cuniculus |
25 | - |
- |
Oryctolagus cuniculus |
25 | - |
purified enzyme | Oryctolagus cuniculus |
Storage Stability | Organism |
---|---|
-80°C, 3-4 weeks | Oryctolagus cuniculus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(5Z,13E)-(15S)-11alpha,15-dihydroxy-9-oxoprost-5,13-dienoate + NAD+ | - |
Oryctolagus cuniculus | (5Z,13E)-11alpha-hydroxy-9,15-dioxoprost-5,13-dienoate + NADH + H+ | - |
? | |
(5Z,13E)-(15S)-11alpha,15-dihydroxy-9-oxoprost-5,13-dienoate + NAD+ | first step in prostaglandin catabolism | Oryctolagus cuniculus | (5Z,13E)-11alpha-hydroxy-9,15-dioxoprost-5,13-dienoate + NADH + H+ | - |
? | |
(5Z,13E)-(15S)-11alpha,15-dihydroxy-9-oxoprost-5,13-dienoate + NAD+ | i.e. 15-hydroxyprostaglandin or (15S)-PGE2, best substrate | Oryctolagus cuniculus | (5Z,13E)-11alpha-hydroxy-9,15-dioxoprost-5,13-dienoate + NADH + H+ | - |
? | |
11alpha,15(S)-dihydroxy-9-ketoprost-5,13-dienoic acid + NAD(P)+ | specific for NAD+ | Oryctolagus cuniculus | 11alpha-hydroxy-9,15-diketoprost-5,13-dienoic acid + NADH | - |
? | |
6-keto-prostaglandin E1 + NAD+ | - |
Oryctolagus cuniculus | ? + NADH | - |
? | |
6-keto-prostaglandin F1alpha + NAD+ | - |
Oryctolagus cuniculus | ? + NADH | - |
? | |
6-ketoprostaglandin E1 + NAD+ | - |
Oryctolagus cuniculus | 6,15-diketoprostaglandin E1 | - |
? | |
6-ketoprostaglandin F1alpha + NAD+ | - |
Oryctolagus cuniculus | 6,15-diketoprostaglandin F1alpha + NADH + H+ | - |
? | |
additional information | substrate specificity, overview, no activity with prostaglandin B1 | Oryctolagus cuniculus | ? | - |
? | |
prostaglandin A1 + NAD+ | - |
Oryctolagus cuniculus | 15-ketoprostaglandin A1 + NADH + H+ | - |
? | |
prostaglandin A1 + NAD+ | - |
Oryctolagus cuniculus | ? + NADH | - |
? | |
prostaglandin D2 + NAD+ | - |
Oryctolagus cuniculus | 15-ketoprostaglandin D2 + NADH + H+ | - |
? | |
prostaglandin D2 + NAD+ | - |
Oryctolagus cuniculus | ? + NADH | - |
? | |
prostaglandin E1 + NAD+ | - |
Oryctolagus cuniculus | ? + NADH | - |
? | |
prostaglandin F2alpha + NAD+ | specific for NAD+ | Oryctolagus cuniculus | 15-ketoprostaglandin F2alpha + NADH + H+ | - |
? | |
prostaglandin F2alpha + NAD+ | - |
Oryctolagus cuniculus | ? + NADH | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 29000, SDS-PAGE | Oryctolagus cuniculus |
Synonyms | Comment | Organism |
---|---|---|
15-hydroxyprostaglandin dehydrogenase | - |
Oryctolagus cuniculus |
NAD-dependent 15-hydroxyprostaglandin dehydrogenase | - |
Oryctolagus cuniculus |
PGDH | - |
Oryctolagus cuniculus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
10 | 10.4 | - |
Oryctolagus cuniculus |
10 | 10.4 | prostaglandin E1, 3-(cyclohexylamine)propanesulfonic acid buffer | Oryctolagus cuniculus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
9.4 | 11 | - |
Oryctolagus cuniculus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | specific for NAD+ | Oryctolagus cuniculus | |
NAD+ | specific for | Oryctolagus cuniculus |