Inhibitors | Comment | Organism | Structure |
---|---|---|---|
CO32- | - |
Klebsiella aerogenes |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.18 | - |
NADH | - |
Klebsiella aerogenes | |
0.42 | - |
NAD+ | - |
Klebsiella aerogenes | |
6.25 | - |
sorbitol 6-phosphate | - |
Klebsiella aerogenes | |
10.5 | - |
D-fructose 6-phosphate | - |
Klebsiella aerogenes |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Klebsiella aerogenes | - |
enzyme induced by growth on D-sorbitol | - |
Purification (Comment) | Organism |
---|---|
approximately 100 fold purification | Klebsiella aerogenes |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Klebsiella aerogenes |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-dulcitol 6-phosphate + NAD+ | at 3% of reaction reaction with sorbitol 6-phosphate | Klebsiella aerogenes | ? | - |
? | |
D-sorbitol 6-phosphate + NAD+ | high specificity for D-sorbitol 6-phosphate | Klebsiella aerogenes | D-fructose 6-phosphate + NADH + H+ | - |
r |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
Tris-HCl buffer | Klebsiella aerogenes |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
in Tris buffer 32times greater activity than in NaHCO3 buffer | Klebsiella aerogenes |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | inhibited by alteration of the 6-amino position of the adenine moiety of NAD+ | Klebsiella aerogenes |