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Literature summary for 1.1.1.138 extracted from

  • Voegele, R.T.; Hahn, M.; Lohaus, G.; Link, T.; Heiser, I.; Mendgen, K.
    Possible roles for mannitol and mannitol dehydrogenase in the biotrophic plant pathogen Uromyces fabae (2005), Plant Physiol., 137, 190-198.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
gene MAD1, expression analysis in fungal tissue, recombinant expression in yeast Uromyces viciae-fabae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information kinetic and thermodynamic analysis, recombinant enzyme Uromyces viciae-fabae
0.034
-
NADP+ pH 10.0, recombinant enzyme Uromyces viciae-fabae
0.038
-
NADPH pH 5.2, recombinant enzyme Uromyces viciae-fabae
0.804
-
D-fructose pH 5.2, recombinant enzyme Uromyces viciae-fabae
78
-
D-mannitol pH 10.0, recombinant enzyme Uromyces viciae-fabae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-mannitol + NADP+ Uromyces viciae-fabae the enzyme has a dual role in mannitol metabolism, D-mannitol is the main storage sugar in spores D-fructose + NADPH + H+
-
r

Organism

Organism UniProt Comment Textmining
Uromyces viciae-fabae
-
biotrophic plant pathogenic rust fungus, gene MAD1
-

Source Tissue

Source Tissue Comment Organism Textmining
haustorial mother cell
-
Uromyces viciae-fabae
-
haustorium lumen Uromyces viciae-fabae
-
additional information no activity in intercellular hyphae, the pathogen infects leaves of Vicia faba forming haustoria Uromyces viciae-fabae
-
spore
-
Uromyces viciae-fabae
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.4
-
recombinant enzyme, forward reaction Uromyces viciae-fabae
1
-
recombinant enzyme, reverse reaction Uromyces viciae-fabae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-mannitol + NADP+ the enzyme has a dual role in mannitol metabolism, D-mannitol is the main storage sugar in spores Uromyces viciae-fabae D-fructose + NADPH + H+
-
r
D-mannitol + NADP+ the reverse reaction occurs only when D-fructose is provided as substrate, and is preferred Uromyces viciae-fabae D-fructose + NADPH + H+
-
r

Synonyms

Synonyms Comment Organism
MAD1p
-
Uromyces viciae-fabae
mannitol dehydrogenase 1
-
Uromyces viciae-fabae
PIG8p formerly Uromyces viciae-fabae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
reverse reaction, recombinant enzyme Uromyces viciae-fabae
10.5
-
forward reaction, recombinant enzyme Uromyces viciae-fabae

pH Range

pH Minimum pH Maximum Comment Organism
8.5 11 sharp decline below pH 8.5 and above pH 11.0, forward reaction, recombinant enzyme Uromyces viciae-fabae

Cofactor

Cofactor Comment Organism Structure
NADP+
-
Uromyces viciae-fabae
NADPH
-
Uromyces viciae-fabae