Cloned (Comment) | Organism |
---|---|
expressed in E. coli | Cladosporium herbarum |
gene MtDH, DNA and amino acid sequence determination and analysis, expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3) | Cladosporium herbarum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.053 | - |
NADP+ | pH 7.5, 22°C | Cladosporium herbarum | |
0.053 | - |
NADPH | plusminus 0.01 mM, pH 7.5, room temperature | Cladosporium herbarum | |
0.067 | - |
NADPH | pH 7.5, 22°C | Cladosporium herbarum | |
0.067 | - |
NADP+ | plusminus 0.007 mM, pH 7.5, room temperature | Cladosporium herbarum | |
33 | - |
D-mannitol | plusminus 5 mM, pH 7.5, room temperature | Cladosporium herbarum | |
117 | - |
D-fructose | plusminus 20 mM, pH 7.5, room temperature | Cladosporium herbarum | |
230 | - |
D-mannitol | pH 7.5, 22°C | Cladosporium herbarum | |
1170 | - |
D-fructose | pH 7.5, 22°C | Cladosporium herbarum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
28320 | - |
- |
Cladosporium herbarum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-mannitol + NADP+ | Cladosporium herbarum | - |
D-fructose + NADPH + H+ | - |
r | |
additional information | Cladosporium herbarum | the enzyme is the major allergen in the organism causing allergic reaction e.g. in humans | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cladosporium herbarum | P0C0Y5 | - |
- |
Cladosporium herbarum | P0C0Y5 | i.e. Davidiella tassiana, strain 280202 | - |
Purification (Comment) | Organism |
---|---|
native enzyme 13.4fold by ammonium sulfate fractionation, hydrophobic interaction chromatography, and anion exchange chromatography | Cladosporium herbarum |
purified by standard chromatographic methods | Cladosporium herbarum |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
allergenic potential on human skin, overview | Cladosporium herbarum |
277 | - |
purified native enzyme | Cladosporium herbarum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-fructose + NADPH + H+ | - |
Cladosporium herbarum | D-mannitol + NADP+ | - |
r | |
D-mannitol + NADP+ | - |
Cladosporium herbarum | D-fructose + NADPH + H+ | - |
r | |
additional information | the enzyme is the major allergen in the organism causing allergic reaction e.g. in humans | Cladosporium herbarum | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Cla h 8 | - |
Cladosporium herbarum |
Cla h 8 allergen | - |
Cladosporium herbarum |
MtDH | - |
Cladosporium herbarum |
NADP-dependent mannitol dehydrogenase | - |
Cladosporium herbarum |
NADP-dependent MtDH | - |
Cladosporium herbarum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | - |
assay at room temperature, forward reaction | Cladosporium herbarum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at, forward reaction | Cladosporium herbarum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Cladosporium herbarum | |
NADPH | - |
Cladosporium herbarum |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Cladosporium herbarum | - |
- |
5.8 |