Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
48000 | - |
SDS-PAGE | Pseudomonas aeruginosa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas aeruginosa | - |
overexpression of tac promoter controlled algD gene containing different plasmids in mucoid strains FRD1 and PAO568 and non-mucoid strains FRD2 and PAO1 | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
higher enzyme activities after IPTG induction only for pPT3 containing strains, low GDP-mannuronate production with pMMB66EH only detectable in mucoid strains, higher with pPT3 with increase in cells of mucoid and non-mucoid strains with IPTG, alginate formation occurs only in mucoid strains without significant increase with IPTG, later enzyme of alginate biosynthesis suggested to become rate-limiting | Pseudomonas aeruginosa |
0.0004 | - |
+/-0.00008, PAO1/pMMB66EH, mid-exponential, without IPTG | Pseudomonas aeruginosa |
0.00056 | - |
+/-0.00024, PAO1/pMMB66EH, early-stationary, with IPTG | Pseudomonas aeruginosa |
0.00076 | - |
+/-0.00016, FRD2/pMMB66EH, mid-exponential, with IPTG | Pseudomonas aeruginosa |
0.00089 | - |
+/-0.0002, FRD2/pMMB66EH, mid-exponential, without IPTG | Pseudomonas aeruginosa |
0.00091 | - |
+/-0.00042, PAO1/pMMB66EH, mid-exponential, with IPTG | Pseudomonas aeruginosa |
0.00094 | - |
+/-0.00052, FRD2/pMMB66EH, early-stationary, with IPTG | Pseudomonas aeruginosa |
0.00106 | - |
+/-0.00062, PAO1/pMMB66EH, early-stationary, without IPTG | Pseudomonas aeruginosa |
0.0013 | - |
+/-0.00004, FRD2/pMMB66EH, early-stationary, without IPTG | Pseudomonas aeruginosa |
0.00685 | - |
+/-0.00228, FRD1/pMMB66EH, mid-exponential, with IPTG | Pseudomonas aeruginosa |
0.00749 | - |
+/-0.00187, PAO1/pPT3, mid-exponential, without IPTG | Pseudomonas aeruginosa |
0.00936 | - |
+/-0.00063, PAO1/pPT3, early-stationary, without IPTG | Pseudomonas aeruginosa |
0.00968 | - |
+/-0.00155, FRD1/pMMB66EH, mid-exponential, without IPTG | Pseudomonas aeruginosa |
0.00992 | - |
+/-0.00105, PAO568/pMMB66EH, mid-exponential, without IPTG | Pseudomonas aeruginosa |
0.01097 | - |
+/-0.00195, FRD2/pPT4, early-stationary, with IPTG | Pseudomonas aeruginosa |
0.01232 | - |
+/-0.00397, PAO568/pMMB66EH, mid-exponential, with IPTG | Pseudomonas aeruginosa |
0.01249 | - |
+/-0.00113, FRD2/pPT4, early-stationary, without IPTG | Pseudomonas aeruginosa |
0.01283 | - |
+/-0.00348, FRD2/pPT3, early-stationary, without IPTG | Pseudomonas aeruginosa |
0.01312 | - |
+/-0.0031, FRD2/pPT3, mid-exponential, without IPTG | Pseudomonas aeruginosa |
0.01401 | - |
+/-0.00147, PAO1/pPT4, early-stationary, with IPTG | Pseudomonas aeruginosa |
0.01436 | - |
+/-0.00303, FRD1/pPT3, mid-exponential, without IPTG | Pseudomonas aeruginosa |
0.01472 | - |
+/-0.00135, PAO1/pPT4, early-stationary, without IPTG | Pseudomonas aeruginosa |
0.01886 | - |
+/-0.00526, PAO568/pMMB66EH, early-stationary, without IPTG | Pseudomonas aeruginosa |
0.01891 | - |
+/-0.0001, PAO568/pPT3, mid-exponential, without IPTG | Pseudomonas aeruginosa |
0.02322 | - |
+/-0.00432, PAO568/pMMB66EH, early-stationary, with IPTG | Pseudomonas aeruginosa |
0.02418 | - |
+/-0.00628, FRD1/pMMB66EH, early-stationary, without IPTG | Pseudomonas aeruginosa |
0.02514 | - |
+/-0.00274, PAO568/pPT4, early-stationary, without IPTG | Pseudomonas aeruginosa |
0.02542 | - |
+/-0.00139, PAO568/pPT4, early-stationary, with IPTG | Pseudomonas aeruginosa |
0.02574 | - |
+/-0.00581, FRD1/pMMB66EH, early-stationary, with IPTG | Pseudomonas aeruginosa |
0.02671 | - |
+/-0.00459, FRD1/pPT4, early-stationary, with IPTG | Pseudomonas aeruginosa |
0.02905 | - |
+/-0.00427, FRD1/pPT4, early-stationary, without IPTG | Pseudomonas aeruginosa |
0.02994 | - |
+/-0.0066, PAO568/pPT3, early-stationary, without IPTG | Pseudomonas aeruginosa |
0.03208 | - |
+/-0.00629, FRD1/pPT3, early-stationary, without IPTG | Pseudomonas aeruginosa |
0.1259 | - |
+/-0.02878, PAO1/pPT3, mid-exponential, with IPTG | Pseudomonas aeruginosa |
0.1364 | - |
+/-0.01055, FRD2/pPT3, mid-exponential, with IPTG | Pseudomonas aeruginosa |
0.1627 | - |
+/-0.03188, PAO568/pPT3, mid-exponential, with IPTG | Pseudomonas aeruginosa |
0.1758 | - |
+/-0.03971, FRD1/pPT3, mid-exponential, with IPTG | Pseudomonas aeruginosa |
0.1919 | - |
+/-0.00268, PAO568/pPT3, early-stationary, with IPTG | Pseudomonas aeruginosa |
0.2037 | - |
+/-0.06998, PAO1/pPT3, early-stationary, with IPTG | Pseudomonas aeruginosa |
0.2097 | - |
+/-0.03274, FRD1/pPT3, early-stationary, with IPTG | Pseudomonas aeruginosa |
0.2196 | - |
+/-0.05517, FRD2/pPT3, early-stationary, with IPTG | Pseudomonas aeruginosa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
GDP-mannose + NAD+ + H2O | - |
Pseudomonas aeruginosa | GDP-mannuronate + NADH | - |
ir |
Subunits | Comment | Organism |
---|---|---|
hexamer | - |
Pseudomonas aeruginosa |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Pseudomonas aeruginosa |