Application | Comment | Organism |
---|---|---|
analysis | enzymatic cycling assay for nicotinic acid adenine dinucleotide phosphate | Neurospora crassa |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Neurospora crassa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
18 | - |
L-arabinitol | pH 8.0, 25°C | Neurospora crassa | |
35 | - |
adonitol | pH 8.0, 25°C | Neurospora crassa | |
174 | - |
NAD+ | pH 8.0, 25°C | Neurospora crassa | |
290 | - |
xylitol | pH 8.0, 25°C | Neurospora crassa |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | two ions per subunit | Neurospora crassa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
39000 | - |
4 * 39000, SDS-PAGE, 4 * 39600, calculated | Neurospora crassa |
39600 | - |
4 * 39000, SDS-PAGE, 4 * 39600, calculated | Neurospora crassa |
152000 | - |
gel filtration | Neurospora crassa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Neurospora crassa | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant protein | Neurospora crassa |
Storage Stability | Organism |
---|---|
22°C, stable for more than 1 month | Neurospora crassa |
4°C, stable for several months | Neurospora crassa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
adonitol + NAD+ | - |
Neurospora crassa | ? + NADH | - |
? | |
L-arabinitol + NAD+ | - |
Neurospora crassa | L-xylulose + NADH + H+ | - |
? | |
additional information | no substrate: D-mannitol, D-arabinitol, D-sorbitol | Neurospora crassa | ? | - |
? | |
Xylitol + NAD+ | - |
Neurospora crassa | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 39000, SDS-PAGE, 4 * 39600, calculated | Neurospora crassa |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | 55 | - |
Neurospora crassa |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
4 h, more than 60% residual activity | Neurospora crassa |
50 | - |
half-life 45 min | Neurospora crassa |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
15 | - |
xylitol | pH 8.0, 25°C | Neurospora crassa | |
18 | - |
adonitol | pH 8.0, 25°C | Neurospora crassa | |
20.1 | - |
NAD+ | pH 8.0, 25°C | Neurospora crassa | |
20.2 | - |
L-arabinitol | pH 8.0, 25°C | Neurospora crassa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9.5 | - |
- |
Neurospora crassa |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
8 | 10.5 | more than 60% of maximal activity within | Neurospora crassa |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | strong preference for NAD+ | Neurospora crassa | |
NADH | - |
Neurospora crassa | |
NADP+ | very low yet detectable activity with NADP+, strong preference for NAD+ | Neurospora crassa | |
NADPH | - |
Neurospora crassa |