Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.1.1.12 extracted from

  • Sullivan, R.; Zhao, H.
    Cloning, characterization, and mutational analysis of a highly active and stable L-arabinitol 4-dehydrogenase from Neurospora crassa (2007), Appl. Microbiol. Biotechnol., 77, 845-852.
    View publication on PubMed

Application

Application Comment Organism
analysis enzymatic cycling assay for nicotinic acid adenine dinucleotide phosphate Neurospora crassa

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Neurospora crassa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
18
-
L-arabinitol pH 8.0, 25°C Neurospora crassa
35
-
adonitol pH 8.0, 25°C Neurospora crassa
174
-
NAD+ pH 8.0, 25°C Neurospora crassa
290
-
xylitol pH 8.0, 25°C Neurospora crassa

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ two ions per subunit Neurospora crassa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
39000
-
4 * 39000, SDS-PAGE, 4 * 39600, calculated Neurospora crassa
39600
-
4 * 39000, SDS-PAGE, 4 * 39600, calculated Neurospora crassa
152000
-
gel filtration Neurospora crassa

Organism

Organism UniProt Comment Textmining
Neurospora crassa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant protein Neurospora crassa

Storage Stability

Storage Stability Organism
22°C, stable for more than 1 month Neurospora crassa
4°C, stable for several months Neurospora crassa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
adonitol + NAD+
-
Neurospora crassa ? + NADH
-
?
L-arabinitol + NAD+
-
Neurospora crassa L-xylulose + NADH + H+
-
?
additional information no substrate: D-mannitol, D-arabinitol, D-sorbitol Neurospora crassa ?
-
?
Xylitol + NAD+
-
Neurospora crassa ?
-
?

Subunits

Subunits Comment Organism
tetramer 4 * 39000, SDS-PAGE, 4 * 39600, calculated Neurospora crassa

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45 55
-
Neurospora crassa

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
4 h, more than 60% residual activity Neurospora crassa
50
-
half-life 45 min Neurospora crassa

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
15
-
xylitol pH 8.0, 25°C Neurospora crassa
18
-
adonitol pH 8.0, 25°C Neurospora crassa
20.1
-
NAD+ pH 8.0, 25°C Neurospora crassa
20.2
-
L-arabinitol pH 8.0, 25°C Neurospora crassa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9.5
-
-
Neurospora crassa

pH Range

pH Minimum pH Maximum Comment Organism
8 10.5 more than 60% of maximal activity within Neurospora crassa

Cofactor

Cofactor Comment Organism Structure
NAD+ strong preference for NAD+ Neurospora crassa
NADH
-
Neurospora crassa
NADP+ very low yet detectable activity with NADP+, strong preference for NAD+ Neurospora crassa
NADPH
-
Neurospora crassa