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Literature summary for 1.1.1.119 extracted from

  • Sovic, D.; Lester, L.; Murray, E.; Cohenford, M.
    The utilization of bathocuproinedisulfonic acid as a reagent for determining D-glucose and D-galactose levels in glycoconjugates (2008), Bioorg. Chem., 36, 91-95.
    View publication on PubMed

Application

Application Comment Organism
analysis method for determination of D-glucose- and D-galactose levels in glycoconjugates. The NAD(P)H produced from the enzymatic oxidation of the monosaccharides reacts with a CuSO4-bathocuproinedisulfonic acid reagent to produce a color complex absorbing maximally at 486 nm. With galactose dehydrogenase and glucose dehydrogenase serving as the model enzymes, reaction analysis gives a linear plot from 2.5 to 250 nmol of sugar. Method has been applicated to sugar released by acid hydrolysis from lactose, porcine submaxillary mucin and raffinose was quantified Pseudomonas fluorescens

Organism

Organism UniProt Comment Textmining
Pseudomonas fluorescens
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Source Tissue

Source Tissue Comment Organism Textmining
commercial preparation
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Pseudomonas fluorescens
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