Application | Comment | Organism |
---|---|---|
synthesis | production of L-ascorbic acid and secretion into culture medium by overexpression of enzyme and arabinone-1,4-lactone oxidase in Saccharomyces cerevisiae and Zygosaccharomyces bailii | Saccharomyces cerevisiae |
Cloned (Comment) | Organism |
---|---|
gene ARA1, co-overexpression of the enzyme and the D-arabinono-1,4-lactone oxidase and/or L-galactono-1,4-lactone dehydrogenase in Saccharomyces cerevisiae strains result in overproduction and accumulation of vitamin C in the culture medium, while overexpression of the D-arabinose dehydrogenase alone does not alter the L-ascorbate content, overview | Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-arabinose + NAD+ | Saccharomyces cerevisiae | - |
D-arabinono-1,4-lactone + NADH | - |
? | |
D-arabinose + NAD+ | Saccharomyces cerevisiae GRF18U | - |
D-arabinono-1,4-lactone + NADH | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
recombinant enzyme overexpressed in Saccharomyces cerevisiae and Zygosaccharomyces bailii | - |
Saccharomyces cerevisiae | - |
gene ARA1 | - |
Saccharomyces cerevisiae GRF18U | - |
gene ARA1 | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-arabinose + NAD+ | - |
Saccharomyces cerevisiae | D-arabinono-1,4-lactone + NADH | - |
? | |
D-arabinose + NAD+ | - |
Saccharomyces cerevisiae GRF18U | D-arabinono-1,4-lactone + NADH | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ARA | - |
Saccharomyces cerevisiae |
D-arabinose dehydrogenase | - |
Saccharomyces cerevisiae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Saccharomyces cerevisiae |