KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0142 | - |
NADH | - |
Clostridium sporogenes | |
0.075 | - |
p-hydroxyphenylpyruvate | - |
Clostridium sporogenes | |
0.106 | - |
phenylpyruvate | - |
Clostridium sporogenes | |
0.195 | - |
NAD+ | - |
Clostridium sporogenes | |
0.691 | - |
indoleglycolate | - |
Clostridium sporogenes | |
1.03 | - |
indolelactate | - |
Clostridium sporogenes | |
1.77 | - |
p-hydroxyphenyllactate | - |
Clostridium sporogenes | |
2.21 | - |
Phenyllactate | - |
Clostridium sporogenes |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
indolelactate + NAD+ | Clostridium sporogenes | - |
indolepyruvate + NADH | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Clostridium sporogenes | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate, DEAE-cellulose | Clostridium sporogenes |
Storage Stability | Organism |
---|---|
-20°C, 100 mM potassium phosphate, pH 7.5, 1 mM mercaptoethanol, 6 months, no loss of activity, 0°C, 100 mM potassium phosphate, pH 7.5, 1 mM mercaptoethanol, 15 days, no loss of activity | Clostridium sporogenes |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
indoleglycolate + NAD+ | - |
Clostridium sporogenes | indoleglyoxylate + NADH | - |
r | |
indolelactate + NAD+ | - |
Clostridium sporogenes | indolepyruvate + NADH | - |
r | |
p-hydroxyphenyllactate + NAD+ | - |
Clostridium sporogenes | p-hydroxyphenylpyruvate + NADH | - |
r | |
phenyllactate + NAD+ | - |
Clostridium sporogenes | phenylpyruvate + NADH | - |
r |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | 8 | indolepyruvate reduction | Clostridium sporogenes |
8 | 9 | indolelactate oxidation | Clostridium sporogenes |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Clostridium sporogenes |