KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.013 | - |
NADH | potassium phosphate buffer, pH 7 | Pseudomonas putida | |
0.067 | - |
NADH | Tris-HCl buffer, pH 9 | Pseudomonas putida | |
0.2 | - |
NAD+ | - |
Pseudomonas putida | |
1.1 | - |
3-dehydrocarnitine | potassium phosphate buffer, pH 7 | Pseudomonas putida | |
1.54 | - |
3-dehydrocarnitine | Tris-HCl buffer, pH 9 | Pseudomonas putida | |
6.25 | - |
L-carnitine | - |
Pseudomonas putida |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
32000 | - |
2 * 32000, SDS-PAGE | Pseudomonas putida |
60000 | - |
gel filtration | Pseudomonas putida |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R)-3-hydroxy-4-trimethylaminobutyrate + NAD+ | Pseudomonas putida | - |
3-dehydrocarnitine + NADH + H+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate, Sephadex G-200, 5'-AMP-Sepharose 4B, gel filtration | Pseudomonas putida |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
55.3 | - |
- |
Pseudomonas putida |
240 | - |
reduction of 3-dehydrocarnitine at pH 7-7.5 | Pseudomonas putida |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R)-3-hydroxy-4-trimethylaminobutyrate + NAD+ | - |
Pseudomonas putida | 3-dehydrocarnitine + NADH + H+ | - |
r | |
carnitine + NAD+ | - |
Pseudomonas putida | 3-dehydrocarnitine + NADH + H+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 32000, SDS-PAGE | Pseudomonas putida |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
activity declines rapidly above 35°C | Pseudomonas putida |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
10 | 30 | - |
Pseudomonas putida |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 7.5 | reduction of 3-dehydrocarnitine | Pseudomonas putida |
9 | - |
oxidation of L-carnitine | Pseudomonas putida |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Pseudomonas putida |