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Literature summary for 1.1.1.103 extracted from
- Identification and characterization of protein encoded by orf382 as L-threonine dehydrogenase (2014), J. Microbiol. Biotechnol., 24, 748-755.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) cells | Escherichia coli |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00802 | - |
n-butanol | at pH 8.8 and 37°C | Escherichia coli |  |
| 0.01129 | - |
L-threonine | at pH 8.8 and 37°C | Escherichia coli | |
| 0.2225 | - |
ethanol | at pH 8.8 and 37°C | Escherichia coli | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Iron | the protein contains 0.424 mol of Fe per mol | Escherichia coli | |
| Zn2+ | the protein contains 0.914 mol of Zn2+ per mol | Escherichia coli | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 40000 | - |
x * 40000, SDS-PAGE | Escherichia coli |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | P07913 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni-NTA agarose column chromatography | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| citric acid + NAD+ | low activity | Escherichia coli | ? | - |
? | |
| D-glucose + NAD+ | - |
Escherichia coli | ? | - |
? | |
| D-threonine + NAD+ | lowest activity | Escherichia coli | (2R)-2-amino-3-oxobutanoate + NADH + H+ | - |
? | |
| ethanol + NAD+ | high activity | Escherichia coli | acetaldehyde + NADH + H+ | - |
? | |
| glycerol + NAD+ | - |
Escherichia coli | ? | - |
? | |
| isopropanol + NAD+ | - |
Escherichia coli | isopropylaldehyde + NADH + H+ | - |
? | |
| L-serine + NAD+ | - |
Escherichia coli | 3-oxo-L-alanine + NADH + H+ | - |
? | |
| L-threonine + NAD+ | high activity | Escherichia coli | (2S)-2-amino-3-oxobutanoate + NADH + H+ | - |
? | |
| lactic acid + NAD+ | low activity | Escherichia coli | ? | - |
? | |
| methanol + NAD+ | low activity | Escherichia coli | formaldehyde + NADH + H+ | - |
? | |
| n-butanol + NAD+ | highest activity | Escherichia coli | butanal + NADH + H+ | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 40000, SDS-PAGE | Escherichia coli |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| L-threonine dehydrogenase | - |
Escherichia coli |
| orf382 | - |
Escherichia coli |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 39 | - |
- |
Escherichia coli |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9.8 | - |
- |
Escherichia coli |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | the enzyme exhibits an apparent preference for NAD+ over NADP+ | Escherichia coli | |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Escherichia coli | isoelectric focusing | - |
5.4 |
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