Cloned (Comment) | Organism |
---|---|
plasmid pTZ-tdh transformed in Escherichia coli DH5alpha competent cells. NcoI-EcoRI restriction fragment inserted into the pET-8c expression vector at the corresponding sites. The resulting plasmid pET-tdh expressed in Escherichia coli strain BL21(DE3)CodonPlus-RIL | Thermococcus kodakarensis KOD1 |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | retains 65% of its original activity after dialysis at 48°C against a buffer containing 1 mM EDTA. Activity is lost when TDH is heated at 60°C for 40 min and in boiling water for 5 min in the presence of 1 mM EDTA | Thermococcus kodakarensis KOD1 |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.028 | - |
NAD+ | at 50°C | Thermococcus kodakarensis KOD1 | |
1.6 | - |
L-threonine | at 50°C | Thermococcus kodakarensis KOD1 |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 0.1 mM reactivates EDTA-100% inhibited enzyme by 40% | Thermococcus kodakarensis KOD1 | |
Co2+ | 0.1 mM reactivates EDTA-100% inhibited enzyme by 54% | Thermococcus kodakarensis KOD1 | |
Cu2+ | 0.1 mM reactivates EDTA-100% inhibited enzyme by 63% | Thermococcus kodakarensis KOD1 | |
Mg2+ | 0.1 mM reactivates EDTA-100% inhibited enzyme by 27% | Thermococcus kodakarensis KOD1 | |
Mn2+ | 0.1 mM reactivates EDTA-100% inhibited enzyme by 38% | Thermococcus kodakarensis KOD1 | |
Ni2+ | 0.1 mM reactivates EDTA-100% inhibited enzyme by 30% | Thermococcus kodakarensis KOD1 | |
Zn2+ | four zinc-binding cysteine residues, 0.1 mM reactivates EDTA-100% inhibited enzyme by 75%, 0.05 mM reactivates by 100% | Thermococcus kodakarensis KOD1 |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
38100 | - |
sequence analysis | Thermococcus kodakarensis KOD1 |
154000 | - |
by gel filtration | Thermococcus kodakarensis KOD1 |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermococcus kodakarensis KOD1 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
by heat treatment (about 80% pure recombinant TDH protein) and gel filtration | Thermococcus kodakarensis KOD1 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-serine + NAD+ | - |
Thermococcus kodakarensis KOD1 | L-2-amino-3-oxopropionate + NADH + H+ | - |
? | |
L-threonine + NAD+ | - |
Thermococcus kodakarensis KOD1 | (2S)-2-amino-3-oxobutanoate + NADH + H+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
L-threonine dehydrogenase | - |
Thermococcus kodakarensis KOD1 |
TDH | - |
Thermococcus kodakarensis KOD1 |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
90 | - |
- |
Thermococcus kodakarensis KOD1 |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
85 | - |
the enzyme shows a half life of 2 h at 85°C and 24 min in boiling water | Thermococcus kodakarensis KOD1 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
12 | - |
- |
Thermococcus kodakarensis KOD1 |