Literature summary for 1.1.1.103 extracted from

Bao, Y.; Xie, H.; Shan, J.; Jiang, R.; Zhang, Y.; Guo, L.; Zhang, R.; Li, Y.
Biochemical characteristics and function of a threonine dehydrogenase encoded by ste11 in Ebosin biosynthesis of Streptomyces sp. 139 (2009), J. Appl. Microbiol., 106, 1140-1146.

Search for more literature for 1.1.1.103

Cloned(Commentary)

Cloned (Comment)	Organism
into pET-30a digested with KpnIEcoRI to construct pET-ste11 and expressed in Escherichia coli BL21	Streptomyces sp. 139

Protein Variants

Protein Variants	Comment	Organism
additional information	ste11 gene knocked out with a double crossover via homologous recombination. Monosaccharide composition of exopolysaccharide produced by the mutant strain is altered from that of ebosin. Bioactivity of the mutant is lower than ebosin	Streptomyces sp. 139

Organism

Organism	UniProt	Comment	Textmining
Streptomyces sp. 139	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
by sonication, centrifugation and on a Ni-NTA resin column	Streptomyces sp. 139

Storage Stability

Storage Stability	Organism
-20°C	Streptomyces sp. 139

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
L-threonine + NAD+	-	Streptomyces sp. 139	(2S)-2-amino-3-oxobutanoate + NADH + H+	-	?
additional information	ste11 gene encoding a TDH may function as a modifier gene of ebosin during its biosynthesis	Streptomyces sp. 139	?	-	?

Synonyms

Synonyms	Comment	Organism
TDH	-	Streptomyces sp. 139
threonine dehydrogenase	-	Streptomyces sp. 139

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
45	-	-	Streptomyces sp. 139

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
25	45	enzyme shows 50% activity at 25°C compared with that at 35°C	Streptomyces sp. 139

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.5	-	-	Streptomyces sp. 139

pH Stability

pH Stability	pH Stability Maximum	Comment	Organism
5.5	7.5	activity at pH 5.5 is 50% of that at pH 7.5	Streptomyces sp. 139

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Release 2023.1 (January 2023)