Cloned (Comment) | Organism |
---|---|
orf PH0655, expression in Escherichia coli | Pyrococcus horikoshii |
Crystallization (Comment) | Organism |
---|---|
purified recombinant enzyme, hanging drop vapour diffusion method, 4°C, 10 mg/ml protein in 50 mM Tris-HCl, pH 7.5, 0.0015 ml of protein and reservoir solution are mixed, the reservoir solution contains 0.2 M NaCl, 0.1 M HEPES, pH 7.5, and 40% v/v PEG 400, 5 days, X-ray diffraction structure determination and preliminary analysis at 2.2 A resolution | Pyrococcus horikoshii |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | required | Pyrococcus horikoshii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
37700 | - |
x * 37700, recombinant enzyme, SDS-PAGE | Pyrococcus horikoshii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrococcus horikoshii | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme from Escherichia coli | Pyrococcus horikoshii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the enzyme is an NAD+-dependent zinc-containing medium-chain enzyme | Pyrococcus horikoshii | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 37700, recombinant enzyme, SDS-PAGE | Pyrococcus horikoshii |
Synonyms | Comment | Organism |
---|---|---|
L-threonine dehydrogenase | - |
Pyrococcus horikoshii |
TDH | - |
Pyrococcus horikoshii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
hyperthermophilic enzyme | Pyrococcus horikoshii |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | dependent on | Pyrococcus horikoshii |