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Literature summary for 1.1.1.103 extracted from

  • Kazuoka, T.; Takigawa, S.; Arakawa, N.; Hizukuri, Y.; Muraoka, I.; Oikawa, T.; Soda, K.
    Novel psychrophilic and thermolabile L-threonine dehydrogenase from psychrophilic Cytophaga sp. strain KUC-1 (2003), J. Bacteriol., 185, 4483-4489.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
-
Cytophaga sp.

Inhibitors

Inhibitors Comment Organism Structure
adenosine-5'-diphosphoribose
-
Cytophaga sp.
CuCl2 1 mM, 100% inhibition Cytophaga sp.
HgCl2 10 mM, 100% inhibition Cytophaga sp.
monoiodoacetate 10 mM, 100% inhibition Cytophaga sp.
N-ethylmaleimide 10 mM, 48% inhibition Cytophaga sp.
NADH competitive to NAD+, noncompetitive to L-threonine Cytophaga sp.
p-chloromercuribenzoic acid 10 mM, 44% inhibition Cytophaga sp.
pyruvate competitive to L-threonine Cytophaga sp.
ZnCl2 1 mM, 72% inhibition Cytophaga sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.56
-
L-threonine pH 9.0, 20°C Cytophaga sp.
3.81
-
L-threonine pH 9.0, 10°C Cytophaga sp.
5.96
-
L-threonine pH 9.0, 30°C Cytophaga sp.
11.2
-
L-threonine pH 9.0, 40°C Cytophaga sp.
19.5
-
L-threonine pH 9.0, 50°C Cytophaga sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35000
-
4 * 35000, SDS-PAGE Cytophaga sp.
139000
-
gel filtration Cytophaga sp.

Organism

Organism UniProt Comment Textmining
Cytophaga sp. Q8KZM4
-
-
Cytophaga sp. KUC-1 Q8KZM4
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Cytophaga sp.

Reaction

Reaction Comment Organism Reaction ID
L-threonine + NAD+ = L-2-amino-3-oxobutanoate + NADH + H+ ordered bi-bi mechanism, NAD+ binds prior to L-threonine Cytophaga sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
91.4
-
pH 9.0, 10°C Cytophaga sp.
156
-
pH 9.0, 20°C Cytophaga sp.
284
-
pH 9.0, 30°C Cytophaga sp.
611
-
pH 9.0, 50°C Cytophaga sp.
722
-
pH 9.0, 40°C Cytophaga sp.

Storage Stability

Storage Stability Organism
-20°C, 10 mM potassium phosphate, pH 7.0, 30% sucrose, 0.1 mM NAD+, 5 mM L-threonine, several months, no loss of activity Cytophaga sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
DL-threo-3-hydroxynorvaline + NAD+ 31% the rate of L-threonine Cytophaga sp. ? + NADH
-
?
DL-threo-3-hydroxynorvaline + NAD+ 31% the rate of L-threonine Cytophaga sp. KUC-1 ? + NADH
-
?
L-threonine + 3-acetyl-pyridine adenine dinucleotide 60.6% the rate of NAD+ Cytophaga sp. L-2-amino-3-oxobutanoate + ?
-
?
L-threonine + 3-acetyl-pyridine adenine dinucleotide 60.6% the rate of NAD+ Cytophaga sp. KUC-1 L-2-amino-3-oxobutanoate + ?
-
?
L-threonine + 3-pyridinealdehyde adenine dinucleotide 7.2% the rate of NAD+ Cytophaga sp. L-2-amino-3-oxobutanoate + ?
-
?
L-threonine + 3-pyridinealdehyde adenine dinucleotide 7.2% the rate of NAD+ Cytophaga sp. KUC-1 L-2-amino-3-oxobutanoate + ?
-
?
L-threonine + NAD+
-
Cytophaga sp. (2S)-2-amino-3-oxobutanoate + NADH + H+
-
?
L-threonine + NAD+
-
Cytophaga sp. KUC-1 (2S)-2-amino-3-oxobutanoate + NADH + H+
-
?
L-threonine + nicotinamide guanine dinucleotide 5.1% the rate of NAD+ Cytophaga sp. L-2-amino-3-oxobutanoate + ?
-
?
L-threonine + thionicotinamide-NAD+ 5.1% the rate of NAD+ Cytophaga sp. L-2-amino-3-oxobutanoate + ?
-
?
additional information specific for L-form of threonine, no substrate: NADP, nicotinic acid adenine dinucleotide, alpha-NAD, nicotinamide hypoxanthine dinucleotide Cytophaga sp. ?
-
?
additional information specific for L-form of threonine, no substrate: NADP, nicotinic acid adenine dinucleotide, alpha-NAD, nicotinamide hypoxanthine dinucleotide Cytophaga sp. KUC-1 ?
-
?

Subunits

Subunits Comment Organism
homotetramer 4 * 35000, SDS-PAGE Cytophaga sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
Cytophaga sp.

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
10 30 60 min, more than 90% residual activity Cytophaga sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
half-life 160 min Cytophaga sp.
45
-
half-life 14 min Cytophaga sp.
50
-
rapid inactivation Cytophaga sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9.5
-
-
Cytophaga sp.

pH Range

pH Minimum pH Maximum Comment Organism
8.5
-
74% of maximum activity Cytophaga sp.
10.5
-
31% of maximum activity Cytophaga sp.

Cofactor

Cofactor Comment Organism Structure
3-acetyl-pyridine adenine dinucleotide
-
Cytophaga sp.
nicotinamide guanine dinucleotide
-
Cytophaga sp.
thionicotinamide-NAD+
-
Cytophaga sp.

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.37
-
adenosine-5'-diphosphoribose substrate NAD+, pH 9.0, 30°C Cytophaga sp.
1.42
-
adenosine-5'-diphosphoribose substrate L-threonine, pH 9.0, 30°C Cytophaga sp.
23.2
-
pyruvate substrate L-threonine, pH 9.0, 30°C Cytophaga sp.
50.7
-
pyruvate substrate NAD+, pH 9.0, 30°C Cytophaga sp.