General Stability | Organism |
---|---|
unstable in the absence of NADPH | Cavia porcellus |
unstable on freezing and thawing | Cavia porcellus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.015 | - |
NADPH | cosubstrate palmitoydihydroxyacetone phosphate | Cavia porcellus | |
0.02 | - |
NADPH | cosubstrate hexadecyldihydroxyacetone phosphate | Cavia porcellus | |
0.021 | - |
Hexadecyldihydroxyacetone phosphate | cosubstrate NADPH | Cavia porcellus | |
1.7 | - |
NADH | - |
Cavia porcellus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
60000 | - |
1 * 60000, SDS-PAGE | Cavia porcellus |
75000 | - |
liver peroxisomes, gel filtration in the presence of octylglucoside | Cavia porcellus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cavia porcellus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
affinity chromatography on NADPH-agarose gel matrix | Cavia porcellus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Cavia porcellus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
62.5 | - |
- |
Cavia porcellus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acyldihydroxyacetone phosphate + NADPH | - |
Cavia porcellus | 1-acyl-sn-glycerol 3-phosphate + NADP+ | - |
? | |
alkyldihydroxyacetone phosphate + NADPH + H+ | - |
Cavia porcellus | 1-alkyl-sn-glycerol 3-phosphate + NADP+ | - |
? | |
hexadecyl dihydroxyacetone phosphate + NADH + H+ | - |
Cavia porcellus | 1-hexadecyl-sn-glycerol 3-phosphate + NAD+ | - |
r | |
hexadecyldihydroxyacetone phosphate + NADPH | - |
Cavia porcellus | 1-hexadecyl-sn-glycerol 3-phosphate + NADP+ | - |
? | |
palmitoylglycerone phosphate + NADPH | i.e. palmitoyldihydroxyacetone phosphate | Cavia porcellus | 1-palmitoylglycerol 3-phosphate + NADP+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 60000, SDS-PAGE | Cavia porcellus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
4 | - |
stable for 4-6 weeks in the presence of octylglucoside and NADPH | Cavia porcellus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | 7.5 | broad optimum | Cavia porcellus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | can substitute for NADPH with very low activity and affinity | Cavia porcellus | |
NADPH | hydrogen from B-side of nicotinamide ring is transferred to reduce keto substrates | Cavia porcellus |