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Literature summary for 1.1.1.101 extracted from
- Purification and properties of acyl/alkyl dihydroxyacetone-phosphate reductase from guinea pig liver peroxisomes (1990), J. Biol. Chem., 265, 8268-8274.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| phosphatidylcholine | micelles, activation, in presence of Triton X-100, slight stimulation at low concentrations | Cavia porcellus |  |
| phosphatidylglycerol | micelles, activation, in presence of Triton X-100, slight stimulation at low concentrations | Cavia porcellus | |
| Triton X-100 | destabilizes during solubilization | Cavia porcellus | |
General Stability
| General Stability | Organism |
|---|---|
| DTT stabilizes during storage | Cavia porcellus |
| freeze-thawing leads to rapid loss of activity | Cavia porcellus |
| high ionic strength facilitates solubilization with neutral or anionic detergents | Cavia porcellus |
| NADPH stabilizes during storage and solubilization | Cavia porcellus |
| Triton X-100, enzyme becomes unstable during solubilization unless NADPH and 1 M KCl are present | Cavia porcellus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| additional information | stable to N-ethylmaleimide | Cavia porcellus | |
| N-ethylmaleimide | not inhibitory | Cavia porcellus | |
| NADP+ | - |
Cavia porcellus | |
| palmitoyl-CoA | competitive to NADPH, not to alkyldihydroxyacetone phosphate | Cavia porcellus | |
| Palmitoyldihydroxyacetone phosphate | competitive to hexadecyldihydroxyacetone phosphate | Cavia porcellus | |
| PCMB | - |
Cavia porcellus | |
| phosphatidylcholine | at high concentrations | Cavia porcellus | |
| phosphatidylethanolamine | - |
Cavia porcellus | |
| phosphatidylglycerol | at high concentrations | Cavia porcellus | |
| phosphatidylinositol | - |
Cavia porcellus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0154 | - |
Palmitoyldihydroxyacetone phosphate | - |
Cavia porcellus | |
| 0.02 | - |
NADPH | - |
Cavia porcellus | |
| 0.021 | - |
Hexadecyldihydroxyacetone phosphate | - |
Cavia porcellus | |
| 1.7 | - |
NADH | - |
Cavia porcellus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 60000 | - |
1 * 60000, SDS-PAGE | Cavia porcellus |
| 70000 | 75000 | gel filtration | Cavia porcellus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acyldihydroxyacetone phosphate + NADPH | Cavia porcellus | enzyme links acyldihydroxyacetone phosphate pathway intermediates to cellular glycerolipid biosynthesis | 1-acyl-sn-glycerol 3-phosphate + NADP+ | - |
? | |
| alkyldihydroxyacetone phosphate + NADPH + H+ | Cavia porcellus | enzyme links acyldihydroxyacetone phosphate pathway intermediates to cellular glycerolipid biosynthesis | 1-alkyl-sn-glycerol 3-phosphate + NADP+ | - |
? | |
Organic Solvent Stability
| Organic Solvent | Comment | Organism |
|---|---|---|
| Glycerol | stabilizes | Cavia porcellus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Cavia porcellus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| affinity chromatography on NADPH-agarose gel matrix | Cavia porcellus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Cavia porcellus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 62.5 | - |
- |
Cavia porcellus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, stable in the presence of DTT and NADPH, 1 month | Cavia porcellus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acyldihydroxyacetone phosphate + NADPH | - |
Cavia porcellus | 1-acyl-sn-glycerol 3-phosphate + NADP+ | - |
? | |
| acyldihydroxyacetone phosphate + NADPH | enzyme links acyldihydroxyacetone phosphate pathway intermediates to cellular glycerolipid biosynthesis | Cavia porcellus | 1-acyl-sn-glycerol 3-phosphate + NADP+ | - |
? | |
| alkyldihydroxyacetone phosphate + NADPH + H+ | - |
Cavia porcellus | 1-alkyl-sn-glycerol 3-phosphate + NADP+ | - |
? | |
| alkyldihydroxyacetone phosphate + NADPH + H+ | enzyme links acyldihydroxyacetone phosphate pathway intermediates to cellular glycerolipid biosynthesis | Cavia porcellus | 1-alkyl-sn-glycerol 3-phosphate + NADP+ | - |
? | |
| hexadecyl dihydroxyacetone phosphate + NADH + H+ | - |
Cavia porcellus | 1-hexadecyl-sn-glycerol 3-phosphate + NAD+ | - |
r | |
| hexadecyldihydroxyacetone phosphate + NADPH | - |
Cavia porcellus | 1-hexadecyl-sn-glycerol 3-phosphate + NADP+ | - |
? | |
| palmitoylglycerone phosphate + NADPH | i.e. palmitoyldihydroxyacetone phosphate | Cavia porcellus | 1-palmitoylglycerol 3-phosphate + NADP+ | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 60000, SDS-PAGE | Cavia porcellus |
| More | 27% hydrophobic amino acids by amino acid analysis | Cavia porcellus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 100 | - |
NADPH | - |
Cavia porcellus | |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADH | can substitute for NADPH | Cavia porcellus | |
| NADPH | hydrogen from B-side of nicotinamide ring is transferred to reduce keto substrates | Cavia porcellus | |
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