Activating Compound | Comment | Organism | Structure |
---|---|---|---|
phosphatidylcholine | micelles, activation, in presence of Triton X-100, slight stimulation at low concentrations | Cavia porcellus | |
phosphatidylglycerol | micelles, activation, in presence of Triton X-100, slight stimulation at low concentrations | Cavia porcellus | |
Triton X-100 | destabilizes during solubilization | Cavia porcellus |
General Stability | Organism |
---|---|
DTT stabilizes during storage | Cavia porcellus |
freeze-thawing leads to rapid loss of activity | Cavia porcellus |
high ionic strength facilitates solubilization with neutral or anionic detergents | Cavia porcellus |
NADPH stabilizes during storage and solubilization | Cavia porcellus |
Triton X-100, enzyme becomes unstable during solubilization unless NADPH and 1 M KCl are present | Cavia porcellus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | stable to N-ethylmaleimide | Cavia porcellus | |
N-ethylmaleimide | not inhibitory | Cavia porcellus | |
NADP+ | - |
Cavia porcellus | |
palmitoyl-CoA | competitive to NADPH, not to alkyldihydroxyacetone phosphate | Cavia porcellus | |
Palmitoyldihydroxyacetone phosphate | competitive to hexadecyldihydroxyacetone phosphate | Cavia porcellus | |
PCMB | - |
Cavia porcellus | |
phosphatidylcholine | at high concentrations | Cavia porcellus | |
phosphatidylethanolamine | - |
Cavia porcellus | |
phosphatidylglycerol | at high concentrations | Cavia porcellus | |
phosphatidylinositol | - |
Cavia porcellus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0154 | - |
Palmitoyldihydroxyacetone phosphate | - |
Cavia porcellus | |
0.02 | - |
NADPH | - |
Cavia porcellus | |
0.021 | - |
Hexadecyldihydroxyacetone phosphate | - |
Cavia porcellus | |
1.7 | - |
NADH | - |
Cavia porcellus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
60000 | - |
1 * 60000, SDS-PAGE | Cavia porcellus |
70000 | 75000 | gel filtration | Cavia porcellus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acyldihydroxyacetone phosphate + NADPH | Cavia porcellus | enzyme links acyldihydroxyacetone phosphate pathway intermediates to cellular glycerolipid biosynthesis | 1-acyl-sn-glycerol 3-phosphate + NADP+ | - |
? | |
alkyldihydroxyacetone phosphate + NADPH + H+ | Cavia porcellus | enzyme links acyldihydroxyacetone phosphate pathway intermediates to cellular glycerolipid biosynthesis | 1-alkyl-sn-glycerol 3-phosphate + NADP+ | - |
? |
Organic Solvent | Comment | Organism |
---|---|---|
Glycerol | stabilizes | Cavia porcellus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cavia porcellus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
affinity chromatography on NADPH-agarose gel matrix | Cavia porcellus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Cavia porcellus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
62.5 | - |
- |
Cavia porcellus |
Storage Stability | Organism |
---|---|
4°C, stable in the presence of DTT and NADPH, 1 month | Cavia porcellus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acyldihydroxyacetone phosphate + NADPH | - |
Cavia porcellus | 1-acyl-sn-glycerol 3-phosphate + NADP+ | - |
? | |
acyldihydroxyacetone phosphate + NADPH | enzyme links acyldihydroxyacetone phosphate pathway intermediates to cellular glycerolipid biosynthesis | Cavia porcellus | 1-acyl-sn-glycerol 3-phosphate + NADP+ | - |
? | |
alkyldihydroxyacetone phosphate + NADPH + H+ | - |
Cavia porcellus | 1-alkyl-sn-glycerol 3-phosphate + NADP+ | - |
? | |
alkyldihydroxyacetone phosphate + NADPH + H+ | enzyme links acyldihydroxyacetone phosphate pathway intermediates to cellular glycerolipid biosynthesis | Cavia porcellus | 1-alkyl-sn-glycerol 3-phosphate + NADP+ | - |
? | |
hexadecyl dihydroxyacetone phosphate + NADH + H+ | - |
Cavia porcellus | 1-hexadecyl-sn-glycerol 3-phosphate + NAD+ | - |
r | |
hexadecyldihydroxyacetone phosphate + NADPH | - |
Cavia porcellus | 1-hexadecyl-sn-glycerol 3-phosphate + NADP+ | - |
? | |
palmitoylglycerone phosphate + NADPH | i.e. palmitoyldihydroxyacetone phosphate | Cavia porcellus | 1-palmitoylglycerol 3-phosphate + NADP+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 60000, SDS-PAGE | Cavia porcellus |
More | 27% hydrophobic amino acids by amino acid analysis | Cavia porcellus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
100 | - |
NADPH | - |
Cavia porcellus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | can substitute for NADPH | Cavia porcellus | |
NADPH | hydrogen from B-side of nicotinamide ring is transferred to reduce keto substrates | Cavia porcellus |