Cloned (Comment) | Organism |
---|---|
overexpression of His-tagged enzyme in Escherichia coli strain BL21(DE3) in inclusion bodies | Plasmodium falciparum |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Plasmodium falciparum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) solubilized from inclusion bodies and refolded is further purified by nickel affinity chromatography | Plasmodium falciparum |
Renatured (Comment) | Organism |
---|---|
solubilization of overexpressed recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) inclusion bodies by 8 M urea, and refolding by dialysis, cation exchange chromatography, and dilution in presence of 0.001 mM NADPH in 20 mM sodium acetate, pH 5.3, 10% glycerol, and 0.05% Tween 20, the refolded enzyme shows 90-95% of soluble enzyme activity | Plasmodium falciparum |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0017 | 0.0018 | recombinant refolded enzyme | Plasmodium falciparum |
0.0019 | - |
recombinant soluble enzyme | Plasmodium falciparum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetoacetyl-CoA + NADPH | - |
Plasmodium falciparum | 3-hydroxybutyryl-CoA + NADP+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
beta-ketoacyl-ACP reductase | - |
Plasmodium falciparum |
FabG | - |
Plasmodium falciparum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Plasmodium falciparum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Plasmodium falciparum |