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Literature summary for 1.1.1.100 extracted from

  • Lai, C.Y.; Cronan, J.E.
    Isolation and characterization of beta-ketoacyl-acyl carrier protein reductase (fabG) mutants of Escherichia coli and Salmonella enterica serovar typhimurium (2004), J. Bacteriol., 186, 1869-1878.
    View publication on PubMedView publication on EuropePMC

Protein Variants

Protein Variants Comment Organism
A154T similar thermal stability as wild-type enzyme Escherichia coli
A154T/E233K temperature-sensitive phenoptype, reductase activity is much more thermolabile than the activity of the wild-type strain Escherichia coli
E233K temperature-sensitive mutant enzyme does not allow growth of Escherichia coli at 42°C in complementation assay Salmonella enterica subsp. enterica serovar Typhimurium
E233K temperature-sensitive phenoptype, reductase activity is much more thermolabile than the activity of the wild-type strain Escherichia coli
M125I/S233T temperature-sensitive mutant enzyme does not allow growth of Escherichia coli at 42°C in complementation assay Salmonella enterica subsp. enterica serovar Typhimurium

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
beta-ketoacyl-[acyl-carrier protein] + NADPH Salmonella enterica subsp. enterica serovar Typhimurium first reductive step in the elongation cycle of fatty acid biosynthesis beta-hydroxyacyl-[acyl-carrier protein] + NADP+
-
?
beta-ketoacyl-[acyl-carrier protein] + NADPH Escherichia coli first reductive step in the elongation cycle of fatty acid biosynthesis beta-hydroxyacyl-[acyl-carrier protein] + NADP+
-
?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-
Salmonella enterica subsp. enterica serovar Typhimurium
-
serovar typhimurium
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetoacetyl-CoA + NADPH + H+
-
Salmonella enterica subsp. enterica serovar Typhimurium D-beta-hydroxybutyryl-CoA + NADP+
-
?
acetoacetyl-CoA + NADPH + H+
-
Escherichia coli D-beta-hydroxybutyryl-CoA + NADP+
-
?
beta-ketoacyl-[acyl-carrier protein] + NADPH first reductive step in the elongation cycle of fatty acid biosynthesis Salmonella enterica subsp. enterica serovar Typhimurium beta-hydroxyacyl-[acyl-carrier protein] + NADP+
-
?
beta-ketoacyl-[acyl-carrier protein] + NADPH first reductive step in the elongation cycle of fatty acid biosynthesis Escherichia coli beta-hydroxyacyl-[acyl-carrier protein] + NADP+
-
?

Synonyms

Synonyms Comment Organism
beta-ketoacyl-acyl carrier protein reductase
-
Salmonella enterica subsp. enterica serovar Typhimurium
beta-ketoacyl-acyl carrier protein reductase
-
Escherichia coli
FabG
-
Salmonella enterica subsp. enterica serovar Typhimurium
FabG
-
Escherichia coli

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30
-
wild-type enzyme: about 15% loss of activity after 15 min, mutant enzyme E233K: about 60% loss of activity after 10 min,mutant enzyme A164T: about 20% loss of activity after 15 min, mutant enzyme A154T/E233K: about 80% loss of activity after 15 min Escherichia coli
37
-
wild-type enzyme: about 40% loss of activity after 15 min, mutant enzyme E233K: about 90% loss of activity after 10 min, mutant enzyme A164T: about 25% loss of activity after 15 min, mutant enzyme A154T/E233K: about 80% loss of activity after 5 min Escherichia coli
45
-
wild-type enzyme: about 70% loss of activity after 15 min, mutant enzyme E233K: complete loss of activity after 10 min, mutant enzyme A164T: about 60% loss of activity after 15 min, mutant enzyme A154T/E233K: complete loss of activity after 5 min Escherichia coli

Cofactor

Cofactor Comment Organism Structure
NADPH
-
Salmonella enterica subsp. enterica serovar Typhimurium
NADPH
-
Escherichia coli