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Literature summary for 1.1.1.10 extracted from

  • Son, l.e..T.; Ko, K.M.; Cho, J.H.; Singaravelu, G.; Chatterjee, I.; Choi, T.W.; Song, H.O.; Yu, J.R.; Park, B.J.; Lee, S.K.; Ahnn, J.
    DHS-21, a dicarbonyl/L-xylulose reductase (DCXR) ortholog, regulates longevity and reproduction in Caenorhabditis elegans (2011), FEBS Lett., 585, 1310-1316.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene dhs-21, DNA and amino acid sequence determination and analysis, the dhs-21 gene is physically mapped to the cosmid R11D1 between the sma-1 and mes-4genes on chromosome V, expression of His6-tagged enzyme. Spatial and temporal expression pattern of the DHS-21-GFP construct in transgenic worms, overview Caenorhabditis elegans

Protein Variants

Protein Variants Comment Organism
additional information library screening and isolation of a dhs-21 deletion mutant Caenorhabditis elegans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.17
-
L-erythrulose pH 7.0, temperature not specified in the publication Caenorhabditis elegans
0.18
-
L-ribulose pH 7.0, temperature not specified in the publication Caenorhabditis elegans
0.27
-
D-erythrose pH 7.0, temperature not specified in the publication Caenorhabditis elegans
0.66
-
L-xylulose pH 7.0, temperature not specified in the publication Caenorhabditis elegans
0.68
-
D-threose pH 7.0, temperature not specified in the publication Caenorhabditis elegans
2.58
-
D-xylulose pH 7.0, temperature not specified in the publication Caenorhabditis elegans

Localization

Localization Comment Organism GeneOntology No. Textmining
plasma membrane of spermatids Caenorhabditis elegans 5886
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-xylulose + NADPH + H+ Caenorhabditis elegans
-
xylitol + NADP+
-
ir
additional information Caenorhabditis elegans dicarbonyl/L-xylulose reductase is bifunctional ?
-
?

Organism

Organism UniProt Comment Textmining
Caenorhabditis elegans Q21929 gene dhs-21
-

Source Tissue

Source Tissue Comment Organism Textmining
gonadal sheath cell
-
Caenorhabditis elegans
-
intestine
-
Caenorhabditis elegans
-
spermathecal-uterine valve
-
Caenorhabditis elegans
-
spermatid
-
Caenorhabditis elegans
-
uterine seam cell
-
Caenorhabditis elegans
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-erythrose + NADPH + H+
-
Caenorhabditis elegans ? + NADP+
-
ir
D-threose + NADPH + H+
-
Caenorhabditis elegans ? + NADP+
-
ir
D-xylulose + NADPH + H+
-
Caenorhabditis elegans xylitol + NADP+
-
?
L-erythrulose + NADPH + H+
-
Caenorhabditis elegans ? + NADP+
-
ir
L-ribulose + NADPH + H+ low activity Caenorhabditis elegans ? + NADP+
-
?
L-xylulose + NADPH + H+
-
Caenorhabditis elegans xylitol + NADP+
-
ir
additional information substrate specificity, overview Caenorhabditis elegans ?
-
?
additional information dicarbonyl/L-xylulose reductase is bifunctional Caenorhabditis elegans ?
-
?

Synonyms

Synonyms Comment Organism
DCXR
-
Caenorhabditis elegans
dicarbonyl/L-xylulose reductase
-
Caenorhabditis elegans

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.49
-
L-ribulose pH 7.0, temperature not specified in the publication Caenorhabditis elegans
1.22
-
D-xylulose pH 7.0, temperature not specified in the publication Caenorhabditis elegans
1.8
-
D-erythrose pH 7.0, temperature not specified in the publication Caenorhabditis elegans
2.32
-
D-threose pH 7.0, temperature not specified in the publication Caenorhabditis elegans
3.32
-
L-erythrulose pH 7.0, temperature not specified in the publication Caenorhabditis elegans
4.98
-
L-xylulose pH 7.0, temperature not specified in the publication Caenorhabditis elegans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
assay at Caenorhabditis elegans

Cofactor

Cofactor Comment Organism Structure
additional information no activity with NADH Caenorhabditis elegans
NADPH
-
Caenorhabditis elegans

Expression

Organism Comment Expression
Caenorhabditis elegans knock-down of daf-16 and elt-2 transcription factors affects dhs-21 expression down

General Information

General Information Comment Organism
malfunction dhs-21 null mutants show defects in longevity, reproduction and egg-laying Caenorhabditis elegans
metabolism dicarbonyl/L-xylulose reductase converts L-xylulose into xylitol, and reduces various alpha-dicarbonyl compounds, performing a dual role in carbohydrate metabolism and detoxification Caenorhabditis elegans
physiological function the enzyme is essential for normal life span and reproduction Caenorhabditis elegans

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.36
-
L-ribulose pH 7.0, temperature not specified in the publication Caenorhabditis elegans
0.47
-
D-xylulose pH 7.0, temperature not specified in the publication Caenorhabditis elegans
3.42
-
D-threose pH 7.0, temperature not specified in the publication Caenorhabditis elegans
6.75
-
D-erythrose pH 7.0, temperature not specified in the publication Caenorhabditis elegans
7.55
-
L-xylulose pH 7.0, temperature not specified in the publication Caenorhabditis elegans
19.03
-
L-erythrulose pH 7.0, temperature not specified in the publication Caenorhabditis elegans