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Literature summary for 1.1.1.10 extracted from

  • Metz, B.; Mojzita, D.; Herold, S.; Kubicek, C.P.; Richard, P.; Seiboth, B.
    A novel L-xylulose reductase essential for L-arabinose catabolism in Trichoderma reesei (2013), Biochemistry, 52, 2453-2460.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.1.1.10

Cloned(Commentary)

Cloned (Comment) Organism
gene lxr3, phylogenetic analysis, cloning in Escherichia coli strain JM109, expression of N-terminal His-tagged in Saccharomyces cerevisiae strain CEN.PK2-1D Trichoderma reesei

Protein Variants

Protein Variants Comment Organism
additional information deletion of gene lxr3 leads to a significant reduction in NADPH specific LXR activity after replacement to both media containing L-arabinose Trichoderma reesei

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.13
-
 NADPH pH 7.0, 30°C, recombinant enzyme, with L-xylulose Trichoderma reesei
16
-
 L-xylulose pH 7.0, 30°C, recombinant enzyme Trichoderma reesei
100
-
 xylitol pH 7.0, 30°C, recombinant enzyme Trichoderma reesei
105
-
 D-ribulose pH 7.0, 30°C, recombinant enzyme Trichoderma reesei
250
-
 D-sorbitol pH 7.0, 30°C, recombinant enzyme Trichoderma reesei

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
31000
-
 x * 31000 Trichoderma reesei

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
xylitol + NADP+ Trichoderma reesei
-
 L-xylulose + NADPH + H+
-
 r
xylitol + NADP+ Trichoderma reesei QM9414
-
 L-xylulose + NADPH + H+
-
 r

Organism

Organism UniProt Comment Textmining
Trichoderma reesei Q8NK50 gene lxr3 or tre60033
-
Trichoderma reesei QM9414 Q8NK50 gene lxr3 or tre60033
-

Purification (Commentary)

Purification (Comment) Organism
recombinant N-terminal His-tagged enzyme from Saccharomyces cerevisiae strain CEN.PK2-1D by nickel affinity chromatography Trichoderma reesei

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-fructose + NADPH + H+ low activity Trichoderma reesei ? + NADP+
-
 ?
D-ribulose + NADPH + H+
-
 Trichoderma reesei ? + NADP+
-
 ?
D-sorbitol + NADP+
-
 Trichoderma reesei D-sorbose + NADPH + H+
-
 ?
D-sorbitol + NADP+
-
 Trichoderma reesei QM9414 D-sorbose + NADPH + H+
-
 ?
D-xylulose + NADPH + H+ low activity Trichoderma reesei xylitol + NADP+
-
 ?
L-sorbose + NADPH + H+ low activity Trichoderma reesei L-sorbitol + NADP+
-
 ?
L-sorbose + NADPH + H+ low activity Trichoderma reesei QM9414 L-sorbitol + NADP+
-
 ?
L-xylulose + NADPH + H+ preferred substrate Trichoderma reesei xylitol + NADP+
-
 r
L-xylulose + NADPH + H+ preferred substrate Trichoderma reesei QM9414 xylitol + NADP+
-
 r
additional information substrate specificity, overview. No activity with L-xylo-3-hexulose, D-sorbose, D-ribitol, D-arabitol, or L-arabitol Trichoderma reesei ?
-
 ?
additional information substrate specificity, overview. No activity with L-xylo-3-hexulose, D-sorbose, D-ribitol, D-arabitol, or L-arabitol Trichoderma reesei QM9414 ?
-
 ?
xylitol + NADP+
-
 Trichoderma reesei L-xylulose + NADPH + H+
-
 r
xylitol + NADP+
-
 Trichoderma reesei QM9414 L-xylulose + NADPH + H+
-
 r

Subunits

Subunits Comment Organism
? x * 31000 Trichoderma reesei

Synonyms

Synonyms Comment Organism
L-xylulose reductase
-
 Trichoderma reesei
LXR3
-
 Trichoderma reesei

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1
-
 xylitol pH 7.0, 30°C, recombinant enzyme Trichoderma reesei
1.8
-
 D-sorbitol pH 7.0, 30°C, recombinant enzyme Trichoderma reesei
7.75
-
 NADPH pH 7.0, 30°C, recombinant enzyme, with L-xylulose Trichoderma reesei
8.24
-
 D-ribulose pH 7.0, 30°C, recombinant enzyme Trichoderma reesei
11.4
-
 L-xylulose pH 7.0, 30°C, recombinant enzyme Trichoderma reesei

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 reduction reaction Trichoderma reesei
9
-
 oxidation reaction Trichoderma reesei

Cofactor

Cofactor Comment Organism Structure
NADP+
-
 Trichoderma reesei
NADPH
-
 Trichoderma reesei

Expression

Organism Comment Expression
Trichoderma reesei transcription of lxr3 is specifically induced by L-arabinose and L-arabitol up

General Information

General Information Comment Organism
evolution L-xylulose reductases belong to the superfamily of short chain dehydrogenases and reductases (SDRs) Trichoderma reesei
malfunction deletion of lxr3 affects growth on L-arabinose and L-arabitol and reduces total NADPH-dependent LXR activity in cell free extracts Trichoderma reesei
metabolism the enzyme catalyzes the NADPH-dependent reduction of L-xylulose to xylitol in L-arabinose and glucuronic acid catabolism Trichoderma reesei
physiological function the L-xylulose reductase is essential for L-arabinose catabolism in Trichoderma reesei Trichoderma reesei