Cloned (Comment) | Organism |
---|---|
expression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) | Mus musculus |
Crystallization (Comment) | Organism |
---|---|
crystal structure analysis and modelling | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
D238E | site-directed mutagenesis, mutant exists in dimeric form at low temperature like the wild-type enzyme resulting in cold inactivation | Mus musculus |
D238E/L242W | site-directed mutagenesis, mutation leads to complete prevention of cold inactivation, mutant exists in tetrameric form at low temperature | Mus musculus |
D238E/L242W/T244C | site-directed mutagenesis, double mutation leads to partial prevention of cold inactivation, mutant exists in dimeric and tetrameric form at low temperature | Mus musculus |
L242W | site-directed mutagenesis, mutation leads to partial prevention of cold inactivation, mutant exists in dimeric and tetrameric form at low temperature | Mus musculus |
L242W/T244C | site-directed mutagenesis, double mutation leads to partial prevention of cold inactivation, mutant exists in dimeric and tetrameric form at low temperature | Mus musculus |
T244C | site-directed mutagenesis, mutant exists in dimeric form at low temperature like the wild-type enzyme resulting in cold inactivation | Mus musculus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Mus musculus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant wild-type and mutant enzymes from Escherichia coli | Mus musculus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
xylitol + NADP+ = L-xylulose + NADPH + H+ | enzyme with dual function showing L-xylulose reductase and dicarbonyl reductase activities, it is probably identical with sperm 34 kDa protein P34H and diacetyl reductase, EC 1.1.1.5 | Mus musculus | |
xylitol + NADP+ = L-xylulose + NADPH + H+ | enzyme with dual function showing L-xylulose reductase and dicarbonyl reductase activities, it is probably identical with sperm 34 kDa protein P34H and diacetyl reductase, EC 1.1.1.5 | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
diacetyl + NAD(P)H | dicarbonyl reductase activity | Mus musculus | acetoin + NAD(P)+ | - |
r | |
diacetyl + NAD(P)H | dicarbonyl reductase activity | Homo sapiens | acetoin + NAD(P)+ | - |
r | |
L-xylulose + NADPH + H+ | - |
Mus musculus | L-xylitol + NADP+ | - |
r | |
L-xylulose + NADPH + H+ | - |
Homo sapiens | L-xylitol + NADP+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
More | the dimeric form is inactive | Mus musculus |
tetramer | amino acid residues Trp242, Glu238, Arg203, and Cys244 are important for tetramer formation | Mus musculus |
tetramer | amino acid residues Trp242, Glu238, Arg203, and Cys244 are important for tetramer formation | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
More | enzyme belongs to the short-chain dehydrogenase/reductase family | Mus musculus |
More | enzyme belongs to the short-chain dehydrogenase/reductase family | Homo sapiens |
XR | - |
Mus musculus |
XR | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Mus musculus |
25 | - |
assay at | Homo sapiens |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
dissociation into inactive dimers at low temperatures | Mus musculus |
additional information | - |
stable at low temperatures | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Mus musculus |
7 | - |
assay at | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Mus musculus | |
NADP+ | - |
Homo sapiens | |
NADPH | - |
Mus musculus | |
NADPH | - |
Homo sapiens |