Literature summary for 1.1.1.1 extracted from

Oh, B.R.; Heo, S.Y.; Lee, S.M.; Hong, W.K.; Park, J.M.; Jung, Y.R.; Kim, D.H.; Sohn, J.H.; Seo, J.W.; Kim, C.H.
Production of 2-butanol from crude glycerol by a genetically-engineered Klebsiella pneumoniae strain (2014), Biotechnol. Lett., 36, 57-62.

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Application

Application	Comment	Organism
synthesis	engineering of Klebsiella pneumoniae to produce 2-butanol from crude glycerol as a sole carbon source by expressing acetolactate synthase (IlvH), keto-acid reducto-isomerase (IlvC) and dihydroxyacid dehydratase (IlvD) from Klebsiella pneumoniae, and alpha-oxoisovalerate decarboxylase (Kivd) and alcohol dehydrogenase (AdhA) from Lactococcus lactis. The engineered strain produce 2-butanol (160 mg/l) from crude glycerol. Elimination of the 2,3-butanediol pathway by inactivating alpha-acetolactate decarboxylase (Adc) further improves the yield of 2-butanol from 160 to 320 mg/l	Lactococcus lactis subsp. lactis

Organism

Organism	UniProt	Comment	Textmining
Lactococcus lactis subsp. lactis	Q9CEN0	-	-
Lactococcus lactis subsp. lactis IL1403	Q9CEN0	-	-

Synonyms

Synonyms	Comment	Organism
AdhA	-	Lactococcus lactis subsp. lactis

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Release 2023.1 (January 2023)