Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Aeropyrum pernix |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0004 | - |
NADH | pH 8.0, 60°C | Aeropyrum pernix | |
0.001 | - |
NAD+ | pH 8.0, 60°C | Aeropyrum pernix | |
0.131 | - |
4-methoxyphenylacetone | pH 8.0, 60°C | Aeropyrum pernix | |
0.147 | - |
1-Hexanol | pH 8.0, 60°C | Aeropyrum pernix | |
0.147 | - |
2-decanone | pH 8.0, 60°C | Aeropyrum pernix | |
0.215 | - |
2-nonanone | pH 8.0, 60°C | Aeropyrum pernix | |
0.286 | - |
2-Octanone | pH 8.0, 60°C | Aeropyrum pernix | |
0.333 | - |
benzaldehyde | pH 8.0, 60°C | Aeropyrum pernix | |
0.396 | - |
1-Pentanol | pH 8.0, 60°C | Aeropyrum pernix | |
0.596 | - |
1-butanol | pH 8.0, 60°C | Aeropyrum pernix | |
0.694 | - |
tert-butyl acetoacetate | pH 8.0, 60°C | Aeropyrum pernix | |
0.703 | - |
Cyclohexanol | pH 8.0, 60°C | Aeropyrum pernix | |
0.752 | - |
2-Pentanol | pH 8.0, 60°C | Aeropyrum pernix | |
1.03 | - |
1-propanol | pH 8.0, 60°C | Aeropyrum pernix | |
1.05 | - |
2-butanol | pH 8.0, 60°C | Aeropyrum pernix | |
1.13 | - |
4-methoxybenzyl alcohol | pH 8.0, 60°C | Aeropyrum pernix | |
1.16 | - |
2-Heptanone | pH 8.0, 60°C | Aeropyrum pernix | |
1.39 | - |
cyclohexanone | pH 8.0, 60°C | Aeropyrum pernix | |
2.44 | - |
2-propanol | pH 8.0, 60°C | Aeropyrum pernix | |
5.01 | - |
2-Hexanone | pH 8.0, 60°C | Aeropyrum pernix | |
5.15 | - |
2-Pentanone | pH 8.0, 60°C | Aeropyrum pernix | |
5.43 | - |
benzyl alcohol | pH 8.0, 60°C | Aeropyrum pernix | |
13.7 | - |
ethanol | pH 8.0, 60°C | Aeropyrum pernix |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
39570 | - |
4 * 39570, calculated from sequence | Aeropyrum pernix |
40000 | - |
4 * 40000, SDS-PAGE | Aeropyrum pernix |
160000 | - |
gel filtration | Aeropyrum pernix |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aeropyrum pernix | Q9Y9P9 | - |
- |
Aeropyrum pernix ATCC 700893 / DSM 11879 / JCM 9820 / NBRC 100138 / K1 | Q9Y9P9 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Aeropyrum pernix |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-butanol + NAD+ | - |
Aeropyrum pernix | butanal + NADH + H+ | - |
? | |
1-butanol + NAD+ | - |
Aeropyrum pernix ATCC 700893 / DSM 11879 / JCM 9820 / NBRC 100138 / K1 | butanal + NADH + H+ | - |
? | |
1-hexanol + NAD+ | - |
Aeropyrum pernix | 1-hexanal + NADH + H+ | - |
? | |
1-pentanol + NAD+ | - |
Aeropyrum pernix | 1-pentanal + NADH + H+ | - |
? | |
1-propanol + NAD+ | - |
Aeropyrum pernix | propanal + NADH + H+ | - |
? | |
1-propanol + NAD+ | - |
Aeropyrum pernix ATCC 700893 / DSM 11879 / JCM 9820 / NBRC 100138 / K1 | propanal + NADH + H+ | - |
? | |
2-butanol + NAD+ | - |
Aeropyrum pernix | 2-butanone + NADH + H+ | - |
? | |
2-butanol + NAD+ | - |
Aeropyrum pernix ATCC 700893 / DSM 11879 / JCM 9820 / NBRC 100138 / K1 | 2-butanone + NADH + H+ | - |
? | |
2-decanone + NADH | - |
Aeropyrum pernix | (S)-2-decanol + NAD+ + H+ | 92% enantiomeric excess | ? | |
2-heptanone + NADH | - |
Aeropyrum pernix | (S)-2-heptanol + NAD+ + H+ | 79% enantiomeric excess | ? | |
2-hexanone + NADH | - |
Aeropyrum pernix | (S)-2-hexanol + NAD+ + H+ | 37% enantiomeric excess | ? | |
2-nonanone + NADH | - |
Aeropyrum pernix | (S)-2-nonanol + NAD+ + H+ | 95% enantiomeric excess | ? | |
2-octanone + NADH | - |
Aeropyrum pernix | (S)-2-octanol + NAD+ + H+ | 92% enantiomeric excess | ? | |
2-pentanol + NAD+ | - |
Aeropyrum pernix | 2-pentanone + NADH + H+ | - |
? | |
2-pentanone + NADH + H+ | - |
Aeropyrum pernix | (S)-2-pentanol + NAD+ | 60% enantiomeric excess | ? | |
2-propanol + NAD+ | - |
Aeropyrum pernix | acetone + NADH + H+ | - |
? | |
2-propanol + NAD+ | - |
Aeropyrum pernix ATCC 700893 / DSM 11879 / JCM 9820 / NBRC 100138 / K1 | acetone + NADH + H+ | - |
? | |
4-methoxybenzyl alcohol + NAD+ | - |
Aeropyrum pernix | 4-methoxybenzaldehyde + NADH + H+ | - |
? | |
4-methoxyphenylacetone + NADH | - |
Aeropyrum pernix | (2S)-1-(4-methoxyphenyl)propan-2-ol + NAD+ + H+ | - |
? | |
benzyl alcohol + NAD+ | i.e. phenylmethanol | Aeropyrum pernix | benzaldehyde + NADH + H+ | - |
? | |
cyclohexanol + NAD+ | - |
Aeropyrum pernix | cyclohexanone + NADH + H+ | - |
? | |
ethanol + NAD+ | - |
Aeropyrum pernix | acetaldehyde + NADH + H+ | - |
? | |
additional information | the enzyme shows broad substrate specificity and prefers aliphatic alcohols and ketones. There are no large differences in the reactivities between primary and secondary alcohols. The enzyme produces (S)-alcohols from the corresponding ketones. The values of the enantiomeric excess increase with the increase of chain length except for the reduction of 2-hexanone. The highest enantioselectivity is shown with the reduction of 2-nonanone | Aeropyrum pernix | ? | - |
? | |
additional information | the enzyme shows broad substrate specificity and prefers aliphatic alcohols and ketones. There are no large differences in the reactivities between primary and secondary alcohols. The enzyme produces (S)-alcohols from the corresponding ketones. The values of the enantiomeric excess increase with the increase of chain length except for the reduction of 2-hexanone. The highest enantioselectivity is shown with the reduction of 2-nonanone | Aeropyrum pernix ATCC 700893 / DSM 11879 / JCM 9820 / NBRC 100138 / K1 | ? | - |
? | |
tert-butyl acetoacetate + NADH + H+ | - |
Aeropyrum pernix | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotetramer | 4 * 40000, SDS-PAGE | Aeropyrum pernix |
homotetramer | 4 * 39570, calculated from sequence | Aeropyrum pernix |
Synonyms | Comment | Organism |
---|---|---|
APE2239 | - |
Aeropyrum pernix |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
95 | - |
catalytic activity increased up to 95°C | Aeropyrum pernix |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
30 min, no loss of activity | Aeropyrum pernix |
80 | - |
30 min, about 20% loss of activity | Aeropyrum pernix |
98 | - |
the enzyme maintains 24% of the original catalytic activity after incubation for 30 min | Aeropyrum pernix |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.071 | - |
4-methoxyphenylacetone | pH 8.0, 60°C | Aeropyrum pernix | |
0.072 | - |
tert-butyl acetoacetate | pH 8.0, 60°C | Aeropyrum pernix | |
0.23 | - |
ethanol | pH 8.0, 60°C | Aeropyrum pernix | |
0.24 | - |
2-propanol | pH 8.0, 60°C | Aeropyrum pernix | |
0.26 | - |
1-propanol | pH 8.0, 60°C | Aeropyrum pernix | |
0.37 | - |
1-Hexanol | pH 8.0, 60°C | Aeropyrum pernix | |
0.4 | - |
NAD+ | pH 8.0, 60°C | Aeropyrum pernix | |
0.4 | - |
2-decanone | pH 8.0, 60°C | Aeropyrum pernix | |
0.41 | - |
NADH | pH 8.0, 60°C | Aeropyrum pernix | |
0.41 | - |
1-butanol | pH 8.0, 60°C | Aeropyrum pernix | |
0.45 | - |
1-Pentanol | pH 8.0, 60°C | Aeropyrum pernix | |
0.48 | - |
2-butanol | pH 8.0, 60°C | Aeropyrum pernix | |
0.52 | - |
Cyclohexanol | pH 8.0, 60°C | Aeropyrum pernix | |
0.6 | - |
4-methoxybenzyl alcohol | pH 8.0, 60°C | Aeropyrum pernix | |
0.6 | - |
2-Pentanol | pH 8.0, 60°C | Aeropyrum pernix | |
0.7 | 1 | 2-nonanone | pH 8.0, 60°C | Aeropyrum pernix | |
0.73 | - |
2-Heptanone | pH 8.0, 60°C | Aeropyrum pernix | |
0.74 | - |
2-Octanone | pH 8.0, 60°C | Aeropyrum pernix | |
0.77 | - |
2-Pentanone | pH 8.0, 60°C | Aeropyrum pernix | |
1.02 | - |
benzyl alcohol | pH 8.0, 60°C | Aeropyrum pernix | |
1.08 | - |
2-Hexanone | pH 8.0, 60°C | Aeropyrum pernix | |
1.22 | - |
benzaldehyde | pH 8.0, 60°C | Aeropyrum pernix | |
1.27 | - |
cyclohexanone | pH 8.0, 60°C | Aeropyrum pernix |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
reduction of 2-pentanone | Aeropyrum pernix |
10.5 | - |
oxidation of 2-pentanol | Aeropyrum pernix |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 9.6 | pH 7.0: about 70% of maximal activity, pH 9.6: about 50% of maximal activity, reduction of 2-pentanone | Aeropyrum pernix |
9.6 | 11.5 | pH 9.5: about 40% of maximal activity, pH 11.5: about 30% of maximal activity, oxidation of 2-pentanol | Aeropyrum pernix |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.017 | - |
ethanol | pH 8.0, 60°C | Aeropyrum pernix | |
0.097 | - |
2-propanol | pH 8.0, 60°C | Aeropyrum pernix | |
0.1 | - |
tert-butyl acetoacetate | pH 8.0, 60°C | Aeropyrum pernix | |
0.15 | - |
2-Pentanone | pH 8.0, 60°C | Aeropyrum pernix | |
0.189 | - |
benzyl alcohol | pH 8.0, 60°C | Aeropyrum pernix | |
0.22 | - |
2-Hexanone | pH 8.0, 60°C | Aeropyrum pernix | |
0.25 | - |
1-propanol | pH 8.0, 60°C | Aeropyrum pernix | |
0.46 | - |
2-butanol | pH 8.0, 60°C | Aeropyrum pernix | |
0.53 | - |
4-methoxybenzyl alcohol | pH 8.0, 60°C | Aeropyrum pernix | |
0.54 | - |
4-methoxyphenylacetone | pH 8.0, 60°C | Aeropyrum pernix | |
0.62 | - |
2-Heptanone | pH 8.0, 60°C | Aeropyrum pernix | |
0.69 | - |
1-butanol | pH 8.0, 60°C | Aeropyrum pernix | |
0.73 | - |
Cyclohexanol | pH 8.0, 60°C | Aeropyrum pernix | |
0.79 | - |
2-Pentanol | pH 8.0, 60°C | Aeropyrum pernix | |
0.91 | - |
cyclohexanone | pH 8.0, 60°C | Aeropyrum pernix | |
1.1 | - |
1-Pentanol | pH 8.0, 60°C | Aeropyrum pernix | |
2.5 | - |
1-Hexanol | pH 8.0, 60°C | Aeropyrum pernix | |
2.6 | - |
2-Octanone | pH 8.0, 60°C | Aeropyrum pernix | |
2.7 | - |
2-decanone | pH 8.0, 60°C | Aeropyrum pernix | |
3.3 | - |
2-nonanone | pH 8.0, 60°C | Aeropyrum pernix | |
3.66 | - |
benzaldehyde | pH 8.0, 60°C | Aeropyrum pernix | |
380 | - |
NAD+ | pH 8.0, 60°C | Aeropyrum pernix | |
1000 | - |
NADH | pH 8.0, 60°C | Aeropyrum pernix |