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Literature summary for 1.1.1.1 extracted from

  • Pennacchio, A.; Rossi, M.; Raia, C.A.
    Synthesis of cinnamyl alcohol from cinnamaldehyde with Bacillus stearothermophilus alcohol dehydrogenase as the isolated enzyme and in recombinant E. coli cells (2013), Appl. Biochem. Biotechnol., 170, 1482-1490.
    View publication on PubMed
Search for more literature for 1.1.1.1

Application

Application Comment Organism
synthesis synthesis of the cinnamyl alcohol by means of enzymatic reduction of cinnamaldehyde using alcohol both as an isolated enzyme, and in recombinant Escherichia coli whole cells in an efficient and sustainable one-phase system. The reduction of cinnamaldehyde (0.5 g/l, 3.8 mmol/l) by the isolated enzyme occurrs in 3 h at 50°C with 97% conversion, and yields high purity cinnamyl alcohol (98%) with a yield of 88% and a productivity of 50 g/g enzyme. The reduction of 12.5 g/l (94 mmol/l) cinnamaldehyde by whole cells in 6 h, at 37°C and no requirement of external cofactor occurrs with 97% conversion, 82% yield of 98% pure alcohol and a productivity of 34 mg/g wet cell weight Geobacillus stearothermophilus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.016
-
 NADH pH 7.7, 60°C Geobacillus stearothermophilus
0.03
-
 cinnamaldehyde pH 7.7, 60°C Geobacillus stearothermophilus
0.11
-
 cinnamyl alcohol pH 7.7, 60°C Geobacillus stearothermophilus
0.45
-
 NAD+ pH 7.7, 60°C Geobacillus stearothermophilus
57.8
-
 2-propanol pH 7.7, 60°C Geobacillus stearothermophilus

Organism

Organism UniProt Comment Textmining
Geobacillus stearothermophilus
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-propanol + NAD+
-
 Geobacillus stearothermophilus aceton + NADH + H+ irreversible, no measurable activity with acetone ir
cinnamaldehyde + NADH + H+
-
 Geobacillus stearothermophilus cinnamyl alcohol + NAD+
-
 r
cinnamyl alcohol + NAD+
-
 Geobacillus stearothermophilus cinnamaldehyde + NADH + H+
-
 r

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
33
-
 NAD+ pH 7.7, 60°C Geobacillus stearothermophilus
43
-
 NADH pH 7.7, 60°C Geobacillus stearothermophilus
43
-
 cinnamyl alcohol pH 7.7, 60°C Geobacillus stearothermophilus
55
-
 cinnamaldehyde pH 7.7, 60°C Geobacillus stearothermophilus
287
-
 2-propanol pH 7.7, 60°C Geobacillus stearothermophilus

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Geobacillus stearothermophilus
NADH
-
 Geobacillus stearothermophilus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
5
-
 2-propanol pH 7.7, 60°C Geobacillus stearothermophilus
73
-
 NAD+ pH 7.7, 60°C Geobacillus stearothermophilus
391
-
 cinnamyl alcohol pH 7.7, 60°C Geobacillus stearothermophilus
1835
-
 cinnamaldehyde pH 7.7, 60°C Geobacillus stearothermophilus
2687
-
 NADH pH 7.7, 60°C Geobacillus stearothermophilus