Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.1.1.1 extracted from

  • Zhao, Q.; Hou, Y.; Gong, G.H.; Yu, M.A.; Jiang, L.; Liao, F.
    Characterization of alcohol dehydrogenase from permeabilized brewers yeast cells immobilized on the derived attapulgite nanofibers (2009), Appl. Biochem. Biotechnol., 160, 2287-2299.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
Glutaraldehyde treating with 0.5% glutaraldehyde solution, the activity of the immobilized enzyme is at maximum Saccharomyces cerevisiae

General Stability

General Stability Organism
ADH immobilized on derived attapulgite nanofibers via glutaraldehyde covalent binding retains higher activity over wider ranges of pH and temperature than those of the free enzyme. After shaking at 125 rpm at 35°C for 32 h, a rapid loss in activity is observed, and almost complete activity of immobilized enzyme is lost in 52 h. The activity of immobilized ADH decreases to 80% of its initial value after four cycles of operation and afterwards gradually decreases with every reuse, but it retains 42% activity after eight cycles for bioreduction of ethyl 3-oxobutyrate. Saccharomyces cerevisiae

Inhibitors

Inhibitors Comment Organism Structure
Glutaraldehyde 71% relative activity in the presence of 10 mM glutaraldehyde Saccharomyces cerevisiae

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ethanol + NAD+
-
Saccharomyces cerevisiae acetaldehyde + NADH + H+
-
?
ethyl 3-oxobutyrate + NADH + H+
-
Saccharomyces cerevisiae ethyl (S)-3-hydroxybutyrate + NAD+
-
?

Synonyms

Synonyms Comment Organism
ADH
-
Saccharomyces cerevisiae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
free enzyme, at 25°C Saccharomyces cerevisiae
35
-
immobilized enzyme Saccharomyces cerevisiae

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
15 50
-
Saccharomyces cerevisiae

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
35 60 58% of the original activity is retained after incubation of the immobilized enzyme at 35°C for 32 h, free enzyme loses 68% activity over a 60 min incubation at 60°C, whereas immobilized ADH retains 44% over a 60 min incubation at 60°C Saccharomyces cerevisiae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
free enzyme, at 25°C Saccharomyces cerevisiae
7.5
-
immobilized enzyme, at 25°C Saccharomyces cerevisiae

pH Range

pH Minimum pH Maximum Comment Organism
5 9
-
Saccharomyces cerevisiae

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 8 at pH 6.0 and 8.0, the activity of free ADH decreases dramatically during the incubation, and 90 min later most of the activity is lost, the immobilized form retains 81% of activity at pH 8.0 Saccharomyces cerevisiae

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Saccharomyces cerevisiae