Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Glutaraldehyde | treating with 0.5% glutaraldehyde solution, the activity of the immobilized enzyme is at maximum | Saccharomyces cerevisiae |
General Stability | Organism |
---|---|
ADH immobilized on derived attapulgite nanofibers via glutaraldehyde covalent binding retains higher activity over wider ranges of pH and temperature than those of the free enzyme. After shaking at 125 rpm at 35°C for 32 h, a rapid loss in activity is observed, and almost complete activity of immobilized enzyme is lost in 52 h. The activity of immobilized ADH decreases to 80% of its initial value after four cycles of operation and afterwards gradually decreases with every reuse, but it retains 42% activity after eight cycles for bioreduction of ethyl 3-oxobutyrate. | Saccharomyces cerevisiae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Glutaraldehyde | 71% relative activity in the presence of 10 mM glutaraldehyde | Saccharomyces cerevisiae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ethanol + NAD+ | - |
Saccharomyces cerevisiae | acetaldehyde + NADH + H+ | - |
? | |
ethyl 3-oxobutyrate + NADH + H+ | - |
Saccharomyces cerevisiae | ethyl (S)-3-hydroxybutyrate + NAD+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ADH | - |
Saccharomyces cerevisiae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
free enzyme, at 25°C | Saccharomyces cerevisiae |
35 | - |
immobilized enzyme | Saccharomyces cerevisiae |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
15 | 50 | - |
Saccharomyces cerevisiae |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | 60 | 58% of the original activity is retained after incubation of the immobilized enzyme at 35°C for 32 h, free enzyme loses 68% activity over a 60 min incubation at 60°C, whereas immobilized ADH retains 44% over a 60 min incubation at 60°C | Saccharomyces cerevisiae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
free enzyme, at 25°C | Saccharomyces cerevisiae |
7.5 | - |
immobilized enzyme, at 25°C | Saccharomyces cerevisiae |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 9 | - |
Saccharomyces cerevisiae |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | 8 | at pH 6.0 and 8.0, the activity of free ADH decreases dramatically during the incubation, and 90 min later most of the activity is lost, the immobilized form retains 81% of activity at pH 8.0 | Saccharomyces cerevisiae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Saccharomyces cerevisiae |