Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Thermoanaerobacter ethanolicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
100000 | - |
SDS-PAGE | Thermoanaerobacter ethanolicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermoanaerobacter ethanolicus | Q0PH30 | strain JW200 | - |
Thermoanaerobacter ethanolicus JW 200 | Q0PH30 | strain JW200 | - |
Purification (Comment) | Organism |
---|---|
DEAE-Sepharose column chromatography | Thermoanaerobacter ethanolicus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2.6 | - |
in Tris-HCl buffer (pH 7.6), 5 mM dithiothreitol, 0.2 mM NADH, and 10 mM acetaldehyde at 65°C | Thermoanaerobacter ethanolicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ethanol + NAD+ | - |
Thermoanaerobacter ethanolicus | acetaldehyde + NADH + H+ | - |
? | |
ethanol + NAD+ | - |
Thermoanaerobacter ethanolicus JW 200 | acetaldehyde + NADH + H+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
AdhE | the purified AdhE exhibits high enzymatic activity attributed to aldehyde dehydrogenase and low alcohol dehydrogenase activity | Thermoanaerobacter ethanolicus |
aldehyde/alcohol dehydrogenase | - |
Thermoanaerobacter ethanolicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Thermoanaerobacter ethanolicus |