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Literature summary for 1.1.1.1 extracted from

  • Kazuoka, T.; Oikawa, T.; Muraoka, I.; Kuroda, S.; Soda, K.
    A cold-active and thermostable alcohol dehydrogenase of a psychrotorelant from Antarctic seawater, Flavobacterium frigidimaris KUC-1 (2007), Extremophiles, 11, 257-267.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
-
Flavobacterium frigidimaris

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline 1 mM, 38% inhibition Flavobacterium frigidimaris
CuCl2 1 mM, 68% inhibition Flavobacterium frigidimaris
FeCl2 1 mM, 57% inhibition Flavobacterium frigidimaris
hexadecyltrimethyl-ammonium bromide 1 mM, 79% inhibition Flavobacterium frigidimaris
HgCl2 1 mM, complete inhibition Flavobacterium frigidimaris
Hydroxylamine hydrochloride 1 mM, 34% inhibition Flavobacterium frigidimaris
iodoacetic acid 1 mM, 96% inhibition Flavobacterium frigidimaris
NEM 1 mM, complete inhibition Flavobacterium frigidimaris
NiCl2 1 mM, 79% inhibition Flavobacterium frigidimaris
PCMB 1 mM, complete inhibition Flavobacterium frigidimaris
polyoxyethylene octylphenyl ether 1 mM, 43% inhibition Flavobacterium frigidimaris
SDS 1 mM, 91% inhibition Flavobacterium frigidimaris

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.147
-
ethanol 20°C, pH 9.0 Flavobacterium frigidimaris
0.168
-
ethanol 30°C, pH 9.0 Flavobacterium frigidimaris
0.19
-
ethanol 10°C, pH 9.0 Flavobacterium frigidimaris
0.248
-
ethanol 40°C, pH 9.0 Flavobacterium frigidimaris
0.43
-
ethanol 50°C, pH 9.0 Flavobacterium frigidimaris
1.04
-
ethanol 60°C, pH 9.0 Flavobacterium frigidimaris
5.7
-
ethanol 20°C, pH 9.0 Saccharomyces cerevisiae
8.56
-
ethanol 30°C, pH 9.0 Saccharomyces cerevisiae
9.03
-
ethanol 10°C, pH 9.0 Saccharomyces cerevisiae
12.1
-
ethanol 40°C, pH 9.0 Saccharomyces cerevisiae

Metals/Ions

Metals/Ions Comment Organism Structure
Zn the enzyme contains 2 gram-atoms Zn per subunit Flavobacterium frigidimaris

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
4 * 40000, SDS-PAGE Flavobacterium frigidimaris
160000
-
gel filtration Flavobacterium frigidimaris

Organism

Organism UniProt Comment Textmining
Flavobacterium frigidimaris Q8L3C9
-
-
Flavobacterium frigidimaris KUC-1 Q8L3C9
-
-
Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Flavobacterium frigidimaris

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
43
-
-
Flavobacterium frigidimaris

Storage Stability

Storage Stability Organism
-20°C, 10 mM potassium phosphate buffer, pH 7.0, containing 0.5 mM NAD+ without loss of activity for several months Flavobacterium frigidimaris

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-butanol + NAD+
-
Flavobacterium frigidimaris butanal + NADH + H+
-
r
1-butanol + NAD+
-
Flavobacterium frigidimaris KUC-1 butanal + NADH + H+
-
r
1-decanol + NAD+
-
Flavobacterium frigidimaris decanal + NADH + H+
-
r
1-decanol + NAD+
-
Flavobacterium frigidimaris KUC-1 decanal + NADH + H+
-
r
1-heptanol + NAD+
-
Flavobacterium frigidimaris heptanal + NADH + H+
-
r
1-heptanol + NAD+
-
Flavobacterium frigidimaris KUC-1 heptanal + NADH + H+
-
r
1-hexanol + NAD+
-
Flavobacterium frigidimaris hexanal + NADH + H+
-
r
1-nonanol + NAD+
-
Flavobacterium frigidimaris nonanal + NADH + H+
-
r
1-octanol + NAD+
-
Flavobacterium frigidimaris octanal + NADH + H+
-
r
1-pentanol + NAD+
-
Flavobacterium frigidimaris pentanal + NADH + H+
-
r
1-propanol + NAD+
-
Flavobacterium frigidimaris propanal + NADH + H+
-
r
2-butanol + NAD+ weak activity Flavobacterium frigidimaris 2-butanone + NADH + H+
-
r
2-decanol + NAD+ weak activity Flavobacterium frigidimaris 2-decanone + NADH + H+
-
r
2-heptanol + NAD+ weak activity Flavobacterium frigidimaris 2-heptanone + NADH + H+
-
r
2-hexanol + NAD+ weak activity Flavobacterium frigidimaris 2-hexanone + NADH + H+
-
r
2-nonanol + NAD+ weak activity Flavobacterium frigidimaris 2-nonanone + NADH + H+
-
r
2-octanol + NAD+ weak activity Flavobacterium frigidimaris 2-octanone + NADH + H+
-
r
2-pentanol + NAD+ weak activity Flavobacterium frigidimaris 2-pentanone + NADH + H+
-
r
2-propanol + NAD+
-
Flavobacterium frigidimaris acetone + NADH + H+
-
r
2-propanol + NAD+
-
Flavobacterium frigidimaris KUC-1 acetone + NADH + H+
-
r
allylalcohol + NAD+
-
Flavobacterium frigidimaris prop-2-enal + NADH + H+
-
?
benzylalcohol + NAD+ weak activity Flavobacterium frigidimaris benzaldehyde + NADH + H+
-
r
ethanol + NAD+
-
Saccharomyces cerevisiae acetaldehyde + NADH + H+
-
r
ethanol + NAD+
-
Flavobacterium frigidimaris acetaldehyde + NADH + H+
-
r
additional information no activity with methanol Flavobacterium frigidimaris ?
-
?
additional information no activity with methanol Flavobacterium frigidimaris KUC-1 ?
-
?

Subunits

Subunits Comment Organism
tetramer 4 * 40000, SDS-PAGE Flavobacterium frigidimaris

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
Saccharomyces cerevisiae
70
-
-
Flavobacterium frigidimaris

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
-
60 0°C: about 70% of maximal activity, about 45% of maximal activity Flavobacterium frigidimaris
20 85 20°C: about 65% of maximal activity, 85°C: about 90% of maximal activity Saccharomyces cerevisiae

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
half-life: 304 min in absence of NAD+ Flavobacterium frigidimaris
60
-
half-life: 2 min in absence of NAD+ Saccharomyces cerevisiae
60
-
half-life: 50 min in absence of NAD+, 143 min in presence of 0.01 mM NAD+ Flavobacterium frigidimaris
65
-
half-life: 20 min in absence of NAD+ Flavobacterium frigidimaris
70
-
half-life: 6 min in absence of NAD+ Flavobacterium frigidimaris

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
39.9
-
ethanol 10°C, pH 9.0 Flavobacterium frigidimaris
62.48
-
ethanol 20°C, pH 9.0 Flavobacterium frigidimaris
109.5
-
ethanol 30°C, pH 9.0 Flavobacterium frigidimaris
160.8
-
ethanol 40°C, pH 9.0 Flavobacterium frigidimaris
251.3
-
ethanol 10°C, pH 9.0 Saccharomyces cerevisiae
262.3
-
ethanol 50°C, pH 9.0 Flavobacterium frigidimaris
432.3
-
ethanol 20°C, pH 9.0 Saccharomyces cerevisiae
474.9
-
ethanol 60°C, pH 9.0 Flavobacterium frigidimaris
786.1
-
ethanol 30°C, pH 9.0 Saccharomyces cerevisiae
895.4
-
ethanol 40°C, pH 9.0 Saccharomyces cerevisiae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
reduction of acetaldehyde Flavobacterium frigidimaris
6.7 7 oxidation of ethanol Flavobacterium frigidimaris

Cofactor

Cofactor Comment Organism Structure
NAD+ specific for NAD+, no activity with NADP+, pro-R stereospecificity for hydrogen transfer Flavobacterium frigidimaris