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Literature summary for 1.1.1.1 extracted from

  • Lai, Y.K.; Chandlee, J.M.; Scandalios, J.G.
    Purification and characterization of three non-allelic alcohol dehydrogenase isoenzymes in maize (1982), Biochim. Biophys. Acta, 706, 9-18.
No PubMed abstract available
Search for more literature for 1.1.1.1

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline
-
 Zea mays
Cu2+ no effect Zea mays
Hg2+ no effect Zea mays
iodoacetamide
-
 Zea mays
NEM
-
 Zea mays
p-hydroxymercuribenzoate
-
 Zea mays
PCMB
-
 Zea mays
PMSF
-
 Zea mays

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information
-
 Zea mays

Metals/Ions

Metals/Ions Comment Organism Structure
Zinc required for activity, tightly bound within the enzyme Zea mays

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35000
-
 2 * 35000, SDS-PAGE Zea mays
70000
-
 gel filtration Zea mays

Organism

Organism UniProt Comment Textmining
Zea mays
-
 3 non-allelic alcohol dehydrogenase isoenzymes
-

Purification (Commentary)

Purification (Comment) Organism
-
 Zea mays

Source Tissue

Source Tissue Comment Organism Textmining
scutellum
-
 Zea mays
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Zea mays

Storage Stability

Storage Stability Organism
-20°C, isoenzyme 2 is stable in 50% ethylene glycol for at least 3 months, isoenzyme 1 and 3 maintain less than 50% of their original activity after 2 weeks Zea mays

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-butene-1-ol + NAD+
-
 Zea mays ? + NADH
-
 ?
2-methylpropan-1-ol + NAD+ weak activity Zea mays 2-methyl-propan-1-one + NADH
-
 ?
3-butene-1-ol + NAD+
-
 Zea mays ? + NADH
-
 ?
3-phenyl-2-propen-1-ol + NAD+
-
 Zea mays ? + NADH
-
 ?
allyl alcohol + NAD+
-
 Zea mays prop-2-en-1-al + NADH
-
 ?
benzyl alcohol + NAD+ no activity Zea mays benzaldehyde + NADH
-
 ?
butan-2-ol + NAD+ weak activity Zea mays butan-2-one + NADH
-
 ?
butanol + NAD+
-
 Zea mays butyraldehyde + NADH
-
 ?
cyclohexanol + NAD+ no activity Zea mays cyclohexanone + NADH
-
 ?
ethanol + NAD+
-
 Zea mays acetaldehyde + NADH
-
 ?
hexan-1-ol + NAD+
-
 Zea mays n-hexanal + NADH
-
 ?
methanol + NAD+ no activity Zea mays formaldehyde + NADH + H+
-
 ?
octan-1-ol + NAD+
-
 Zea mays n-octanal + NADH
-
 ?
pentanol + NAD+
-
 Zea mays n-pentanal + NADH
-
 ?
propanol + NAD+
-
 Zea mays propionaldehyde + NADH + H+
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 35000, SDS-PAGE Zea mays

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
55
-
 60 min, 73% loss of activity of enzyme 1, 10% loss of activity of enzyme 2, 21% loss of activity of enzyme 3 Zea mays

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
10
-
-
 Zea mays

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 9 isoenzyme 2 and isoenzyme 3 Zea mays

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Zea mays
NADH
-
 Zea mays