Information on EC 6.5.1.2 - DNA ligase (NAD+)

New: Word Map on EC 6.5.1.2
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Mark a special word or phrase in this record:
Search Reference ID:
Select one or more organisms in this record:
Show additional data
Do not include text mining results
Include (text mining) results (more...)
Include results (AMENDA + additional results, but less precise; more...)

The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
6.5.1.2
-
RECOMMENDED NAME
GeneOntology No.
DNA ligase (NAD+)
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m = AMP + beta-nicotinamide D-ribonucleotide + (deoxyribonucleotide)n+m
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
esterification
-
-
phosphodiester
-
SYSTEMATIC NAME
IUBMB Comments
Poly(deoxyribonucleotide):poly(deoxyribonucleotide) ligase (AMP-forming, NMN-forming)
Catalyses the formation of a phosphodiester at the site of a single-strand break in duplex DNA. RNA can also act as substrate, to some extent. cf. EC 6.5.1.1, DNA ligase (ATP), EC 6.5.1.6, DNA ligase (ATP or NAD+), and EC 6.5.1.7, DNA ligase (ATP, ADP or GTP).
CAS REGISTRY NUMBER
COMMENTARY hide
37259-52-2
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
strain GR501
-
-
Manually annotated by BRENDA team
strain K12
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
Uniprot
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
strain A3(2)
-
-
Manually annotated by BRENDA team
strain A3(2)
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
strain TAK16D
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
Thermus sp. TAK16D
strain TAK16D
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m
AMP + diphosphate + (deoxyribonucleotide)n+m
show the reaction diagram
-
NAD+-dependent DNA ligase LigN is non-essential for cell viability. Haloferax volcanii also encodes the ATP-dependent DNA ligase LigA. As with LigN, LigA is also non-essential for cell viability. Simultaneous inactivation of both proteins is lethal, however, indicating that they share an essential function
-
-
?
NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m
?
show the reaction diagram
NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m
AMP + beta-nicotinamide D-ribonucleotide + (deoxyribonucleotide)n+m
show the reaction diagram
NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m
AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m
show the reaction diagram
NAD+ + 5'-d(ACCATTCCTGATTCTAAGTG)-3' + 5'-d(CTCAGGTCGACAGTCTGCGG)-3'
?
show the reaction diagram
NAD+ + 5'-d(GCCATTCCTGATTCTAAGTG)-3' + 5'-d(CTCAGGTCGACAGTCTGCGG)-3'
?
show the reaction diagram
NAD+ + deoxyribonucleotide
AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m
show the reaction diagram
NADH + (deoxyribonucleotide)n + (deoxyribonucleotide)m
?
show the reaction diagram
-
NADH has a significantly higher Km as NAD+
-
-
-
Thionicotinamide derivative of NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m
?
show the reaction diagram
-
significantly higher Km as NAD+
-
-
-
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m
AMP + diphosphate + (deoxyribonucleotide)n+m
show the reaction diagram
-
NAD+-dependent DNA ligase LigN is non-essential for cell viability. Haloferax volcanii also encodes the ATP-dependent DNA ligase LigA. As with LigN, LigA is also non-essential for cell viability. Simultaneous inactivation of both proteins is lethal, however, indicating that they share an essential function
-
-
?
NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m
?
show the reaction diagram
NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m
AMP + beta-nicotinamide D-ribonucleotide + (deoxyribonucleotide)n+m
show the reaction diagram
NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m
AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m
show the reaction diagram
NAD+ + deoxyribonucleotide
AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m
show the reaction diagram
-
-
-
-
?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Cu2+
30% of activity obtained with Mg2+
MgCl2
-
highest activity: 10 mM MgCl2
Na+
-
inhibitory
NH4Cl
-
enzymatic activity increased in the presence of monovalent cations, maximal activity at 10 mM NaCl
Rb+
-
stimulates
additional information
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
(29S,34S,36R,37R)-1,1'-(1,3-phenylene)bis(3-((S)-1-((3aR,6S,6aR)-6-(benzyloxy)-2,2-dimethyl-dihydro-5H-furo[3,2-d][1,3]dioxol-5-yl)-3-hydroxypropyl)urea)
-
-
(29S,34S,36R,37R)-1,1'-(1,4-phenylene)bis(3-((S)-1-((3aR,6S,6aR)-6-(benzyloxy)-2,2-dimethyl-dihydro-5H-furo[3,2-d][1,3]dioxol-5-yl)-3-ethoxy-3-oxopropyl)urea)
-
-
(2R,3R,4S,5R)-2-[6-amino-2-(butylsulfanyl)-9H-purin-9-yl]-5-(hydroxymethyl)tetrahydrofuran-3,4-diol
-
-
-
(2R,3R,4S,5R)-2-[6-amino-2-(cyclopentyloxy)-9H-purin-9-yl]-5-(hydroxymethyl)tetrahydrofuran-3,4-diol
(3S)-3-[4-amino-2-[(4-methylcyclohexyl)oxy]-5-oxopyrido[2,3-d]pyrimidin-8(5H)-yl]-4-hydroxycyclopentanecarboxamide
-
-
(3S)-3-[4-amino-2-[(4-methylcyclohexyl)oxy]-5-oxopyrido[2,3-d]pyrimidin-8(5H)-yl]-4-hydroxycyclopentanecarboxylic acid
-
-
(3S,3'R)-3,3'-(propane-1,3-diyldiimino)bis[3-[(3aR,6S,6aR)-6-(benzyloxy)-2,2-dimethyltetrahydrofuro[2,3-d][1,3]dioxol-5-yl]propan-1-ol]
-
-
(4-amino-2-[(2-methylcyclohexyl)oxy]-5-oxopyrido[2,3-d]pyrimidin-8(5H)-yl)acetonitrile
-
-
-
(4R)-N4-(6-chloro-2-methoxyacridin-9-yl)-N1,N1-diethylpentane-1,4-diamine
-
-
(8R,10R)-10-((4S,5S,6S)-4-Amino-5-hydroxy-6-methyl-tetrahydro-pyran-2-yloxy)-6,8,11-trihydroxy-8-hydroxymethoxymethyl-1-methoxy-7,8,9,10-tetrahydro-naphthacene-5,12-dione
-
IC50: 0.005 mM
(NH4)2SO4
-
(NH4)2SO4 concentrations higher than 30 mM are inhibitory. At 100 mM KCl, concentrations of (NH4)2SO4 above 12 mM are inhibitory
1,N6-etheno-NAD+
-
-
12-(6-piperidin-1-ylhexyl)-7,12-dihydro-6H-[1]benzothiepino[5,4-b]indole
-
-
2,2'-([4-[(6-chloro-2-methoxyacridin-9-yl)amino]pentyl]imino)diethanol
-
-
2,4-diamino-5-oxo-5H-chromeno[2,3-b]pyridine-3-carbonitrile
-
powerful inhibitor, exhibits good inhibition at nanomolar concentrations
2,4-diamino-7-dimethylamino-pyrimido[4,5-d]pyrimidine
-
IC50 of 0.0001 mg/ml, specific inhibitor of NAD+-dependent DNA ligase, inhibits competitively with respect to NAD+ and specifically inhibits enzyme adenylation, but not DNA adenylation or ligation
2-(butylsulfanyl)-5'-deoxyadenosine
2-(butylsulfanyl)adenosine
2-(cyclobutylmethoxy)-5'-deoxy-5'-fluoroadenosine
2-(cyclopentyloxy)-5'-deoxy-3'-O-(3,4-dichlorobenzyl)adenosine
2-(cyclopentyloxy)-5'-deoxyadenosine
2-(cyclopentyloxy)-9-[3,5-dideoxy-5-fluoro-3-(2l5-triaz-1-en-2-yn-1-yl)-b-D-xylofuranosyl]-9H-purin-6-amine
2-(pentyloxy)adenosine
2-(pentylsulfanyl)adenosine
2-amino-5-(2-hydroxyethyl)-7-(trifluoromethyl)-1,8-naphthyridine-3-carboxamide
-
-
2-amino-5-(2-methoxyethyl)-7-(trifluoromethyl)-1,8-naphthyridine-3-carboxamide
-
-
2-amino-5-(aminomethyl)-7-(trifluoromethyl)-1,8-naphthyridine-3-carboxamide
-
-
2-amino-5-methyl-7-(trifluoromethyl)-1,8-naphthyridine-3-carboxamide
-
-
2-amino-5-oxo-5H-chromeno[2,3-b]pyridine-3-carboxamide
-
powerful inhibitor, exhibits good inhibition at nanomolar concentrations
2-amino-5-phenyl-7-(trifluoromethyl)-1,8-naphthyridine-3-carboxamide
-
-
2-amino-5-[(3R)-3-aminopyrrolidin-1-yl]-6-bromo-7-(trifluoromethyl)-1,8-naphthyridine-3-carboxamide
-
-
2-amino-5H-chromeno[2,3-b]pyridine-3-carboxamide
-
powerful inhibitor, exhibits good inhibition at nanomolar concentrations
2-amino-6-(2-phenylethyl)-7-(trifluoromethyl)-1,8-naphthyridine-3-carboxamide
-
-
2-amino-6-(hydroxymethyl)-7-(trifluoromethyl)-1,8-naphthyridine-3-carboxamide
-
-
2-amino-6-(propan-2-yl)-7-(trifluoromethyl)-1,8-naphthyridine-3-carboxamide
-
-
2-amino-6-benzyl-7-(trifluoromethyl)-1,8-naphthyridine-3-carboxamide
-
-
2-amino-6-benzyl-7-chloro-5-methyl-1,8-naphthyridine-3-carboxamide
-
-
2-amino-6-bromo-5-(3-hydroxyazetidin-1-yl)-7-(trifluoromethyl)-1,8-naphthyridine-3-carboxamide
-
-
2-amino-6-bromo-5-(4-hydroxypiperidin-1-yl)-7-(trifluoromethyl)-1,8-naphthyridine-3-carboxamide
-
-
2-amino-6-bromo-5-[(2-hydroxyethyl)(methyl)amino]-7-(trifluoromethyl)-1,8-naphthyridine-3-carboxamide
-
-
2-amino-6-bromo-5-[(3R)-3-hydroxypyrrolidin-1-yl]-7-(trifluoromethyl)-1,8-naphthyridine-3-carboxamide
-
-
2-amino-6-bromo-7-(trifluoromethyl)-1,8-naphthyridine-3-carboxamide
-
-
2-amino-6-bromo-7-cyclopentyl-1,8-naphthyridine-3-carboxamide
-
-
2-amino-6-bromo-7-tert-butyl-1,8-naphthyridine-3-carboxamide
-
-
2-amino-6-chloro-5-ethyl-1,8-naphthyridine-3-carboxamide
-
-
2-amino-6-cyclopentyl-7-(trifluoromethyl)-1,8-naphthyridine-3-carboxamide
-
-
2-amino-6-cyclopropyl-7-(trifluoromethyl)-1,8-naphthyridine-3-carboxamide
-
-
2-amino-6-ethenyl-7-(trifluoromethyl)-1,8-naphthyridine-3-carboxamide
-
-
2-amino-6-ethyl-7-(trifluoromethyl)-1,8-naphthyridine-3-carboxamide
-
-
2-amino-6-methyl-7-(trifluoromethyl)-1,8-naphthyridine-3-carboxamide
-
-
2-amino-7,8-difluoro-5-oxo-5H-chromeno[2,3-b]pyridine-3-carboxamide
-
powerful inhibitor, exhibits good inhibition at nanomolar concentrations, worse inhibitor than 2-amino-7-fluoro-5-oxo-4a,10a-dihydro-5H-chromeno[2,3-b]pyridine-3-carboxamide
2-amino-7-(trifluoromethyl)-1,8-naphthyridine-3-carboxamide
-
-
2-amino-7-chloro-5-methyl-6-(prop-2-en-1-yl)-1,8-naphthyridine-3-carboxamide
-
-
2-amino-7-chloro-5-methyl-6-(pyridin-4-ylmethyl)-1,8-naphthyridine-3-carboxamide
-
-
2-amino-7-fluoro-5-oxo-5H-chromeno[2,3-b]pyridine-3-carboxamide
-
powerful inhibitor, exhibits good inhibition at nanomolar concentrations, better inhibitor than 2-amino-7,8-difluoro-5-oxo-4a,10a-dihydro-5H-chromeno[2,3-b]pyridine-3-carboxamide
2-amino-7-[2-(benzyloxy)propan-2-yl]-6-bromo-1,8-naphthyridine-3-carboxamide
-
-
2-bromo-4-(phenylamino)thieno[3,2-c]pyridine-7-carboxamide
2-bromo-4-(propylamino)thieno[3,2-c]pyridine-7-carboxamide
2-bromo-4-[(2-hydroxyethyl)amino]thieno[3,2-c]pyridine-7-carboxamide
2-methylthio ADP
-
-
2-methylthio-ATP
3,3'-(decane-1,10-diylbis(azanediyl))bis(3-(6-(benzyloxy)-2,2-dimethyl-dihydro-5H-furo[3,2-d][1,3]dioxol-5-yl)propan-1-ol)
-
-
3,3'-(propane-1,3-diylbis(azanediyl))bis(3-(6-(benzyloxy)-2,2-dimethyl-dihydro-5H-furo[2,3-d][1,3]dioxol-5-yl)propan-1-ol)
-
-
3-((R)-6-benzyloxy-2,2-dimethyl-tetrahydro-furo[2,3-d][1,3]dioxol-5-yl)-3-(3-phenoxymethyl-thioureido)-propionic acid ethyl ester
-
IC50: 0.004 mM
3-((R)-6-benzyloxy-2,2-dimethyl-tetrahydro-furo[2,3-d][1,3]dioxol-5-yl)-3-[3-(3-{3-[1-((S)-6-benzyloxy-2,2-dimethyl-tetrahydro-furo[2,3-d][1,3]dioxol-5-yl)-2-ethoxycarbonyl-ethyl]-ureido}-phenyl)-ureido]-propionic acid ethyl ester
-
IC50: 0.0277 mM
3-(4-amino-2-[(2-methylcyclohexyl)oxy]-5-oxopyrido[2,3-d]pyrimidin-8(5H)-yl)propanenitrile
-
-
-
3-amino-6-[(6-chloro-2-methoxyacridin-9-yl)amino]heptan-1-ol
-
-
3-chloro-9-[[5-(diethylammonio)pentan-2-yl]amino]-7-methoxyacridin-2-aminium
-
-
3-chloro-N9-[5-(diethylamino)pentan-2-yl]-7-methoxyacridine-2,9-diamine
-
-
4-([4-amino-2-[(2-methylcyclohexyl)oxy]-5-oxopyrido[2,3-d]pyrimidin-8(5H)-yl]methyl)benzoic acid
-
-
4-amino-2-(1,4-dioxaspiro[4.5]dec-8-yloxy)-8-[(1R)-2-hydroxycyclopentyl]pyrido[2,3-d]pyrimidin-5(8H)-one
-
-
4-amino-2-(1H-pyrazol-5-yl)thieno[3,2-c]pyridine-7-carboxamide
4-amino-2-(2-fluoroethoxy)-8-(2-hydroxyethyl)pyrido[2,3-d]pyrimidin-5(8H)-one
-
-
4-amino-2-(cyclopentylamino)-8-(2-hydroxyethyl)pyrido[2,3-d]pyrimidin-5(8H)-one
-
-
4-amino-2-(cyclopentyloxy)-8-(2-hydroxyethyl)pyrido[2,3-d]pyrimidin-5(8H)-one
-
-
4-amino-2-(cyclopentylsulfanyl)-8-(2-hydroxyethyl)pyrido[2,3-d]pyrimidin-5(8H)-one
-
-
4-amino-2-(cyclopentylsulfanyl)-8-[(1R)-2-hydroxycyclopentyl]pyrido[2,3-d]pyrimidin-5(8H)-one
-
-
4-amino-2-bromo-3-methylthieno[3,2-c]pyridine-7-carboxamide
4-amino-2-bromothieno[3,2-c]pyridine-7-carboxamide
4-amino-2-methylthieno[3,2-c]pyridine-7-carboxamide
4-amino-2-[(1,3-difluoropropan-2-yl)oxy]-8-(2-hydroxyethyl)pyrido[2,3-d]pyrimidin-5(8H)-one
-
-
4-amino-2-[(2,4-dimethylcyclohexyl)oxy]-8-[(1R)-2-hydroxycyclopentyl]pyrido[2,3-d]pyrimidin-5(8H)-one
-
-
4-amino-2-[(2-methylcyclohexyl)oxy]-8-(1H-pyrazol-4-ylmethyl)pyrido[2,3-d]pyrimidin-5(8H)-one
-
-
4-amino-2-[(2-methylcyclohexyl)oxy]-8-[(1R,5R)-7-oxo-6-oxabicyclo[3.2.1]oct-4-yl]pyrido[2,3-d]pyrimidin-5(8H)-one
-
-
4-amino-2-[(4-chlorocyclohexyl)oxy]-8-[(1R)-2-hydroxycyclopentyl]pyrido[2,3-d]pyrimidin-5(8H)-one
-
-
4-amino-3-(1H-pyrazol-5-yl)thieno[3,2-c]pyridine-7-carboxamide
4-amino-3-bromothieno[3,2-c]pyridine-7-carboxamide
4-amino-3-methylthieno[3,2-c]pyridine-7-carboxamide
4-amino-6-(1H-benzimidazol-2-yl)pyridazin-3(2H)-one
-
-
4-amino-8-(2,3-dihydroxypropyl)-2-[(2-methylcyclohexyl)oxy]pyrido[2,3-d]pyrimidin-5(8H)-one
-
-
4-amino-8-(2-hydroxyethyl)-2-(2-methylcyclohexyl)pyrido[2,3-d]pyrimidin-5(8H)-one
-
-
4-amino-8-(2-hydroxyethyl)-2-(4-methylcyclohexyl)pyrido[2,3-d]pyrimidin-5(8H)-one
-
-
4-amino-8-(2-hydroxyethyl)-2-(propan-2-yloxy)pyrido[2,3-d]pyrimidin-5(8H)-one
-
-
4-amino-8-(3-fluoro-2-hydroxypropyl)-2-[(2-methylcyclohexyl)oxy]pyrido[2,3-d]pyrimidin-5(8H)-one
-
-
4-amino-8-(cyclopent-3-en-1-ylmethyl)-2-[(2-methylcyclohexyl)oxy]pyrido[2,3-d]pyrimidin-5(8H)-one
-
-
4-amino-8-[(1R)-2-hydroxycyclopentyl]-2-[[4-(trifluoromethyl)cyclohexyl]oxy]pyrido[2,3-d]pyrimidin-5(8H)-one
-
-
4-amino-8-[(1S)-2,3-dihydroxycyclopentyl]-2-[(4-methylcyclohexyl)oxy]pyrido[2,3-d]pyrimidin-5(8H)-one
-
-
4-amino-8-[(1S,2S)-2-hydroxycyclopentyl]-2-[(4-methylcyclohexyl)oxy]pyrido[2,3-d]pyrimidin-5(8H)-one
-
-
4-amino-8-[(2R)-2-hydroxycyclohexyl]-2-[(2-methylcyclohexyl)oxy]pyrido[2,3-d]pyrimidin-5(8H)-one
-
-
4-amino-8-[(2R)-2-hydroxycyclopentyl]-2-[(4-methylcyclohexyl)oxy]pyrido[2,3-d]pyrimidin-5(8H)-one
-
-
4-amino-8-[(2S,5S)-2,5-dihydroxycyclopentyl]-2-[(4-methylcyclohexyl)oxy]pyrido[2,3-d]pyrimidin-5(8H)-one
-
-
4-amino-8-[(3,4-dihydroxycyclopentyl)methyl]-2-[(2-methylcyclohexyl)oxy]pyrido[2,3-d]pyrimidin-5(8H)-one
-
-
4-amino-8-[[(2R)-2-hydroxycyclopentyl]methyl]-2-[(2-methylcyclohexyl)oxy]pyrido[2,3-d]pyrimidin-5(8H)-one
-
-
4-amino-8-[[(4S)-4-hydroxytetrahydrofuran-3-yl]methyl]-2-[(2-methylcyclohexyl)oxy]pyrido[2,3-d]pyrimidin-5(8H)-one
-
-
4-amino-N2-ethylthieno[3,2-c]pyridine-2,7-dicarboxamide
4-aminothieno[3,2-c]pyridine-2,7-dicarboxamide
4-aminothieno[3,2-c]pyridine-3,7-dicarboxamide
4-aminothieno[3,2-c]pyridine-7-carboxamide
5'-deoxy-2-(pentyloxy)adenosine
5'-deoxy-2-(spiro[2.2]pent-1-ylmethoxy)adenosine
5'-deoxy-2-phenoxyadenosine
5'-deoxy-2-[(trans-4-methylcyclohexyl)oxy]adenosine
5'-deoxy-5'-fluoro-2-(pentyloxy)adenosine
5'-deoxy-5'-fluoro-2-[(trans-4-methylcyclohexyl)oxy]adenosine
5'-deoxy-5'-fluoro-2-[[1-(hydroxymethyl)cyclopropyl]methoxy]adenosine
5-([4-amino-2-[(4-methylcyclohexyl)oxy]-5-oxopyrido[2,3-d]pyrimidin-8(5H)-yl]methyl)thiophene-2-carboxylic acid
-
-
5-[[[(4-chlorophenyl)amino]carbonyl](phenylmethyl)amino]-5,6-dideoxy-1,2-O-(1-methylethylidene)-3-O-(phenylmethyl)-alpha-L-gluco-heptofuranuronic acid ethyl ester
-
IC50: 0.00965 mM
6-chloro-2-methoxy-N-[4-(piperidin-1-yl)pentyl]acridin-9-amine
-
-
6-chloro-9-[[5-(diethylammonio)pentan-2-yl]amino]-2-methoxyacridinium
-
-
7-methoxy-6-methylpteridine-2,4-diamine
-
-
9-(3-chloro-3,5-dideoxy-beta-D-xylofuranosyl)-2-(cyclopentyloxy)-9H-purin-6-amine
9-(3-chloro-3,5-dideoxy-beta-D-xylofuranosyl)-2-(spiro[2.2]pent-1-ylmethoxy)-9H-purin-6-amine
9-(3-chloro-3-deoxy-beta-D-xylofuranosyl)-2-(cyclopentyloxy)-9H-purin-6-amine
ADP
-
inhibits adenylation reaction
AMP
-
inhibits adenylation reaction
cAMP
-
inhibits adenylation reaction
Chloroquine
Chloroquine diphosphate
-
IC50: 0.0014 mM
deoxyribonucleotide
-
-
-
doxorubicin
ethyl 3-(1-benzyl-3-(4-chlorophenyl)ureido)-3-(6-(benzyloxy)-2,2-dimethyl-dihydro-5H-furo[3,2-d][1,3]dioxol-5-yl)propanoate
-
-
ethyl 3-(3-benzoylthioureido)-3-(6-(benzyloxy)-2,2-dimethyl-dihydro-5H-furo[3,2-d][1,3]dioxol-5-yl)propanoate
-
-
ethyl 4-amino-7-carbamoylthieno[3,2-c]pyridine-2-carboxylate
ethyl-3-O-benzyl-5,6-dideoxy-1,2-O-(1-methylethylidene)-5-[[(phenylcarbonyl)carbamoyl]amino]-alpha-L-xylo-heptofuranuronate
-
-
ethyl-3-O-benzyl-5-[benzyl[(4-chlorophenyl)carbamoyl]amino]-5,6-dideoxy-1,2-O-(1-methylethylidene)-alpha-L-xylo-heptofuranuronate
-
-
hydroxychloroquine
KCl
-
inhibition by KCl occurs at greater than 30 mM (NH4)2SO
methotrexate
-
IC50 of 0.008 mg/ml
Mn2+
-
activation at 0.2-1.0 mM, inhibition at higher concentration
N'-(6-chloro-2-methoxyacridin-9-yl)-N,N-diethylbutane-1,4-diamine
-
-
N'-(6-chloro-2-methoxyacridin-9-yl)-N,N-dimethylbutane-1,4-diamine
-
-
N,N'-bis[4-((4-(diethylamino)-1-methylbutyl)amino)-quinoline-6-yl]sebacamide
-
powerful inhibitor, exhibits good inhibition at nanomolar concentrations
N,N'-bis[4-((4-(diethylamino)-1-methylbutyl)amino)-quinoline-8-yl]adipamide
-
powerful inhibitor, exhibits good inhibition at nanomolar concentrations
N,N'-bis[[(3aR,6S,6aR)-6-(benzyloxy)-2,2-dimethyltetrahydrofuro[2,3-d][1,3]dioxol-5-yl]methyl]dodecane-1,12-diamine
-
-
N,N-bis(4-aminobutyl)-N'-(6-chloro-2-methoxyacridin-9-yl)butane-1,4-diamine
-
-
N,N-bis(4-aminobutyl)-N'-(6-chloro-2-methoxyacridin-9-yl)pentane-1,5-diamine
-
-
N-(4-aminobutyl)-N-(3-aminopropyl)-N'-(6-chloro-2-methoxyacridin-9-yl)butane-1,4-diamine
-
-
N-(4-aminobutyl)-N-(3-aminopropyl)-N'-(6-chloro-2-methoxyacridin-9-yl)pentane-1,5-diamine
-
-
N-(4-aminobutyl)-N-[3-[(6-chloro-2-methoxyacridin-9-yl)amino]propyl]butane-1,4-diamine
-
-
N-(6-chloro-2-methoxyacridin-9-yl)butane-1,4-diamine
-
-
N-[6-piperidin-1-ylhexyl]-6,7-dihydrobenzo[b]indolo[2,3-d]thiepine
-
LX competes with NAD+ andf inhibits the enzyme with IC50 in the low micromolar range, LX exhibits around 15fold better inhibition of the Mycobacterium tuberculosis enzyme compared to the human DNA ligase
N1,N1-bis(2-chloroethyl)-N4-(6-chloro-2-methoxyacridin-9-yl)pentane-1,4-diamine
-
-
N1-(7-chloro-[4]quinolyl)-N4,N4-bis-(2-hydroxy-ethyl)-1-methyl-butanediyldiamine
-
IC50: 0.046 mM
N4-(7-chloro-7,8-dihydroquinolin-4-yl)-N1,N1-diethylpentane-1,4-diamine
-
-
N4-(7-chloroquinolin-4-yl)-N1,N1-diethylpentame-1,4-diamine
-
-
Na+
-
above 20-150 mM
NAD+
-
inhibitory above 0.1 mM, activity is abolished at 0.5 mM
pyridochromanone
-
IC50: 0.0006 mM
pyridochromanones
-
differently substituted compounds tested and all found to be inhibitory, competitive with NAD+, inhibitory in nano-molar rage without affecting human DNA-ligase (ATP-depentend) activity
-
Quinacrine
Rp-cAMPS
-
-
Sp-cAMPS
-
-
tert-butyl 3-(7-amino-3-bromo-6-carbamoyl-1,8-naphthyridin-2-yl)azetidine-1-carboxylate
-
-
additional information
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
(NH4)2SO4
-
with 30 mM (NH4)2SO4, the activity is maximal
bovine serum albumin
0.025 mg/ml, stimulating
-
dithiothreitol
10 mM, stimulating
Macromolecular solutes
-
blunt-end ligation in presence of high concentrations of macromolecular solutes, inactive in absence, e.g. polyethylene glycol 6000, bovine plasma albumin, or Ficoll 70
-
NaCl
highest activity at 200 mM
NH4Cl
highest activity at 200 mM
Polyamines
-
blunt-end ligation are linear oligomers. Optimal concentration: caldopentamine, 0.05 mM, thermine, 0.1-0.2 mM, spermine, 0.2 mM, thermospermine, 0.4 mM, sperminediol, 0.75 mM
-
Polyethylene glycol
-
in presence of high molecular weight polyethylene glycol 20000, 6000 or 1000, 8-28%, the enzyme catalyzes blunt-end intermolecular joining to yield linear oligomers, but no circular DNA forms. In presence of low molecular polyethylene glycols 400, 200, 8-80%, or the monomer ethylene glycol, 16-80%, the circular forms formed by intramolecular ligation are also detected
potassium glutamate
highest activity at 350 mM
additional information
-
no requirement for sulfhydryl reagent
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.14
(dT)10
-
-
0.000025 - 0.00056
5'-phosphate terminus of deoxyribonucleotides
0.000025
5'-phosphoryl termini of poly(dA-dT)
-
-
-
0.0000025
acridinium-ester labelled DNA
-
pH 7.4, 21C
-
0.000028 - 0.000193
deoxyribonucleotide
0.000136
DNA
-
pH 7.6, 21C, C/G matched nick DNA substrate
0.0000185 - 0.076
NAD+
0.00008 - 0.105
nicked DNA
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.27
deoxyribonucleotide
Streptococcus pneumoniae
-
co-substrate: NAD+, mutant L75F, 25C, pH 7.5; co-substrate: NAD+, wild-type, 25C, pH 7.5
-
0.4
DNA
Melanoplus sanguinipes
-
pH 7.6, 21C, C/G matched nick DNA substrate
0.072 - 0.1
NAD+
0.0008 - 0.132
nicked DNA
0.033
nicked substrate
Aquifex aeolicus
O66880
pH 7.6, 65C
-
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0014
2,4-diamino-7-dimethylamino-pyrimido[4,5-d]pyrimidine
-
-
0.000051 - 0.000065
deoxyribonucleotide
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.085
(29S,34S,36R,37R)-1,1'-(1,3-phenylene)bis(3-((S)-1-((3aR,6S,6aR)-6-(benzyloxy)-2,2-dimethyl-dihydro-5H-furo[3,2-d][1,3]dioxol-5-yl)-3-hydroxypropyl)urea)
Mycobacterium tuberculosis
-
-
0.225
(29S,34S,36R,37R)-1,1'-(1,4-phenylene)bis(3-((S)-1-((3aR,6S,6aR)-6-(benzyloxy)-2,2-dimethyl-dihydro-5H-furo[3,2-d][1,3]dioxol-5-yl)-3-ethoxy-3-oxopropyl)urea)
Mycobacterium tuberculosis
-
-
0.0001 - 0.01
(2R,3R,4S,5R)-2-[6-amino-2-(butylsulfanyl)-9H-purin-9-yl]-5-(hydroxymethyl)tetrahydrofuran-3,4-diol
0.00021 - 0.03
(2R,3R,4S,5R)-2-[6-amino-2-(cyclopentyloxy)-9H-purin-9-yl]-5-(hydroxymethyl)tetrahydrofuran-3,4-diol
0.0000488
(3S)-3-[4-amino-2-[(4-methylcyclohexyl)oxy]-5-oxopyrido[2,3-d]pyrimidin-8(5H)-yl]-4-hydroxycyclopentanecarboxamide
Escherichia coli
-
pH and temperature not specified in the publication
0.000051
(3S)-3-[4-amino-2-[(4-methylcyclohexyl)oxy]-5-oxopyrido[2,3-d]pyrimidin-8(5H)-yl]-4-hydroxycyclopentanecarboxylic acid
Escherichia coli
-
pH and temperature not specified in the publication
0.0462
(3S,3'R)-3,3'-(propane-1,3-diyldiimino)bis[3-[(3aR,6S,6aR)-6-(benzyloxy)-2,2-dimethyltetrahydrofuro[2,3-d][1,3]dioxol-5-yl]propan-1-ol]
Mycobacterium tuberculosis
-
-
0.00002
(4-amino-2-[(2-methylcyclohexyl)oxy]-5-oxopyrido[2,3-d]pyrimidin-8(5H)-yl)acetonitrile
Escherichia coli
-
pH and temperature not specified in the publication
-
0.005
(8R,10R)-10-((4S,5S,6S)-4-Amino-5-hydroxy-6-methyl-tetrahydro-pyran-2-yloxy)-6,8,11-trihydroxy-8-hydroxymethoxymethyl-1-methoxy-7,8,9,10-tetrahydro-naphthacene-5,12-dione
Mycobacterium tuberculosis
-
IC50: 0.005 mM
0.12
(Rp)-3',5'-cyclic adenosine monophosphothiolate
Escherichia coli
-
37C, pH 7.4, DNA ligation
0.054
1,N6-etheno-NAD+
Haemophilus influenzae
-
pH and temperature not specified in the publication
0.0135
12-(6-piperidin-1-ylhexyl)-7,12-dihydro-6H-[1]benzothiepino[5,4-b]indole
Mycobacterium tuberculosis
-
-
0.0009
2,4-diamino-5-oxo-5H-chromeno[2,3-b]pyridine-3-carbonitrile
Escherichia coli
-
-
0.00013 - 0.00017
2-(butylsulfanyl)-5'-deoxyadenosine
0.000075 - 0.00127
2-(butylsulfanyl)adenosine
0.000021 - 0.000158
2-(cyclobutylmethoxy)-5'-deoxy-5'-fluoroadenosine
0.00001 - 0.00013
2-(cyclopentyloxy)-5'-deoxy-3'-O-(3,4-dichlorobenzyl)adenosine
0.000019 - 0.00037
2-(cyclopentyloxy)-5'-deoxyadenosine
0.00007 - 0.00009
2-(cyclopentyloxy)-9-[3,5-dideoxy-5-fluoro-3-(2l5-triaz-1-en-2-yn-1-yl)-b-D-xylofuranosyl]-9H-purin-6-amine
0.00015 - 0.00043
2-(pentyloxy)adenosine
0.00038 - 0.00044
2-(pentylsulfanyl)adenosine
0.01
2-amino-5-(2-hydroxyethyl)-7-(trifluoromethyl)-1,8-naphthyridine-3-carboxamide
0.0025
2-amino-5-(aminomethyl)-7-(trifluoromethyl)-1,8-naphthyridine-3-carboxamide
Escherichia coli
-
pH and temperature not specified in the publication
0.0005
2-amino-5-methyl-7-(trifluoromethyl)-1,8-naphthyridine-3-carboxamide
Escherichia coli
-
pH and temperature not specified in the publication
0.0001
2-amino-5-oxo-5H-chromeno[2,3-b]pyridine-3-carboxamide
Escherichia coli
-
-
0.0025
2-amino-5-phenyl-7-(trifluoromethyl)-1,8-naphthyridine-3-carboxamide
Escherichia coli
-
pH and temperature not specified in the publication
0.001
2-amino-5-[(3R)-3-aminopyrrolidin-1-yl]-6-bromo-7-(trifluoromethyl)-1,8-naphthyridine-3-carboxamide
0.0005
2-amino-6-(hydroxymethyl)-7-(trifluoromethyl)-1,8-naphthyridine-3-carboxamide
Escherichia coli
-
pH and temperature not specified in the publication
0.0008
2-amino-6-(propan-2-yl)-7-(trifluoromethyl)-1,8-naphthyridine-3-carboxamide
0.0005
2-amino-6-benzyl-7-chloro-5-methyl-1,8-naphthyridine-3-carboxamide
0.0007
2-amino-6-bromo-5-(4-hydroxypiperidin-1-yl)-7-(trifluoromethyl)-1,8-naphthyridine-3-carboxamide
0.0003
2-amino-6-bromo-7-(trifluoromethyl)-1,8-naphthyridine-3-carboxamide
Escherichia coli
-
pH and temperature not specified in the publication
0.0005
2-amino-6-bromo-7-cyclopentyl-1,8-naphthyridine-3-carboxamide
Escherichia coli
-
pH and temperature not specified in the publication
0.0003
2-amino-6-bromo-7-tert-butyl-1,8-naphthyridine-3-carboxamide
Escherichia coli
-
pH and temperature not specified in the publication
0.008
2-amino-6-chloro-5-ethyl-1,8-naphthyridine-3-carboxamide
Escherichia coli
-
pH and temperature not specified in the publication
0.0025
2-amino-6-cyclopentyl-7-(trifluoromethyl)-1,8-naphthyridine-3-carboxamide
Escherichia coli
-
pH and temperature not specified in the publication
0.0004
2-amino-6-cyclopropyl-7-(trifluoromethyl)-1,8-naphthyridine-3-carboxamide
0.0006
2-amino-6-ethyl-7-(trifluoromethyl)-1,8-naphthyridine-3-carboxamide
Escherichia coli
-
pH and temperature not specified in the publication
0.0005
2-amino-6-methyl-7-(trifluoromethyl)-1,8-naphthyridine-3-carboxamide
Escherichia coli
-
pH and temperature not specified in the publication
0.00008
2-amino-7,8-difluoro-5-oxo-5H-chromeno[2,3-b]pyridine-3-carboxamide
Escherichia coli
-
-
0.0005
2-amino-7-(trifluoromethyl)-1,8-naphthyridine-3-carboxamide
Escherichia coli
-
pH and temperature not specified in the publication
0.0008
2-amino-7-chloro-5-methyl-6-(prop-2-en-1-yl)-1,8-naphthyridine-3-carboxamide
Escherichia coli
-
pH and temperature not specified in the publication
0.001
2-amino-7-chloro-5-methyl-6-(pyridin-4-ylmethyl)-1,8-naphthyridine-3-carboxamide
Escherichia coli
-
pH and temperature not specified in the publication
0.00004
2-amino-7-fluoro-5-oxo-5H-chromeno[2,3-b]pyridine-3-carboxamide
Escherichia coli
-
-
0.005
2-amino-7-[2-(benzyloxy)propan-2-yl]-6-bromo-1,8-naphthyridine-3-carboxamide
Escherichia coli
-
value above, pH and temperature not specified in the publication
0.15 - 0.2
2-bromo-4-(phenylamino)thieno[3,2-c]pyridine-7-carboxamide
0.13 - 0.2
2-bromo-4-(propylamino)thieno[3,2-c]pyridine-7-carboxamide
0.2
2-bromo-4-[(2-hydroxyethyl)amino]thieno[3,2-c]pyridine-7-carboxamide
0.0041
2-methylthio ADP
Escherichia coli
-
37C, pH 7.4, DNA ligation
0.0011
2-methylthio ATP
Escherichia coli
-
37C, pH 7.4, DNA ligation
0.0005
2-methylthio-ATP
Enterococcus faecalis
-
-
0.26
3,3'-(decane-1,10-diylbis(azanediyl))bis(3-(6-(benzyloxy)-2,2-dimethyl-dihydro-5H-furo[3,2-d][1,3]dioxol-5-yl)propan-1-ol)
Mycobacterium tuberculosis
-
-
0.0462
3,3'-(propane-1,3-diylbis(azanediyl))bis(3-(6-(benzyloxy)-2,2-dimethyl-dihydro-5H-furo[2,3-d][1,3]dioxol-5-yl)propan-1-ol)
Mycobacterium tuberculosis
-
-
0.004
3-((R)-6-benzyloxy-2,2-dimethyl-tetrahydro-furo[2,3-d][1,3]dioxol-5-yl)-3-(3-phenoxymethyl-thioureido)-propionic acid ethyl ester
Mycobacterium tuberculosis
-
IC50: 0.004 mM
0.0277
3-((R)-6-benzyloxy-2,2-dimethyl-tetrahydro-furo[2,3-d][1,3]dioxol-5-yl)-3-[3-(3-{3-[1-((S)-6-benzyloxy-2,2-dimethyl-tetrahydro-furo[2,3-d][1,3]dioxol-5-yl)-2-ethoxycarbonyl-ethyl]-ureido}-phenyl)-ureido]-propionic acid ethyl ester
Mycobacterium tuberculosis
-
IC50: 0.0277 mM
0.000036
3-(4-amino-2-[(2-methylcyclohexyl)oxy]-5-oxopyrido[2,3-d]pyrimidin-8(5H)-yl)propanenitrile
Escherichia coli
-
pH and temperature not specified in the publication
-
0.000019
4-([4-amino-2-[(2-methylcyclohexyl)oxy]-5-oxopyrido[2,3-d]pyrimidin-8(5H)-yl]methyl)benzoic acid
Escherichia coli
-
pH and temperature not specified in the publication
0.00009
4-amino-2-(1,4-dioxaspiro[4.5]dec-8-yloxy)-8-[(1R)-2-hydroxycyclopentyl]pyrido[2,3-d]pyrimidin-5(8H)-one
Escherichia coli
-
pH and temperature not specified in the publication
0.029 - 0.2
4-amino-2-(1H-pyrazol-5-yl)thieno[3,2-c]pyridine-7-carboxamide
0.00195
4-amino-2-(2-fluoroethoxy)-8-(2-hydroxyethyl)pyrido[2,3-d]pyrimidin-5(8H)-one
Escherichia coli
-
pH and temperature not specified in the publication
0.01
4-amino-2-(cyclopentylamino)-8-(2-hydroxyethyl)pyrido[2,3-d]pyrimidin-5(8H)-one
Escherichia coli
-
pH and temperature not specified in the publication
0.0051
4-amino-2-(cyclopentyloxy)-8-(2-hydroxyethyl)pyrido[2,3-d]pyrimidin-5(8H)-one
Escherichia coli
-
pH and temperature not specified in the publication
0.0035
4-amino-2-(cyclopentylsulfanyl)-8-(2-hydroxyethyl)pyrido[2,3-d]pyrimidin-5(8H)-one
Escherichia coli
-
pH and temperature not specified in the publication
0.000063
4-amino-2-(cyclopentylsulfanyl)-8-[(1R)-2-hydroxycyclopentyl]pyrido[2,3-d]pyrimidin-5(8H)-one
Escherichia coli
-
pH and temperature not specified in the publication
0.003 - 0.2
4-amino-2-bromo-3-methylthieno[3,2-c]pyridine-7-carboxamide
0.021 - 0.16
4-amino-2-bromothieno[3,2-c]pyridine-7-carboxamide
0.2
4-amino-2-methylthieno[3,2-c]pyridine-7-carboxamide
0.00031
4-amino-2-[(1,3-difluoropropan-2-yl)oxy]-8-(2-hydroxyethyl)pyrido[2,3-d]pyrimidin-5(8H)-one
Escherichia coli
-
pH and temperature not specified in the publication
0.00017
4-amino-2-[(2,4-dimethylcyclohexyl)oxy]-8-[(1R)-2-hydroxycyclopentyl]pyrido[2,3-d]pyrimidin-5(8H)-one
Escherichia coli
-
pH and temperature not specified in the publication
0.000036
4-amino-2-[(2-methylcyclohexyl)oxy]-8-(1H-pyrazol-4-ylmethyl)pyrido[2,3-d]pyrimidin-5(8H)-one
Escherichia coli
-
pH and temperature not specified in the publication
0.000028
4-amino-2-[(2-methylcyclohexyl)oxy]-8-[(1R,5R)-7-oxo-6-oxabicyclo[3.2.1]oct-4-yl]pyrido[2,3-d]pyrimidin-5(8H)-one
Escherichia coli
-
pH and temperature not specified in the publication
0.000041
4-amino-2-[(4-chlorocyclohexyl)oxy]-8-[(1R)-2-hydroxycyclopentyl]pyrido[2,3-d]pyrimidin-5(8H)-one
Escherichia coli
-
pH and temperature not specified in the publication
0.1 - 0.2
4-amino-3-(1H-pyrazol-5-yl)thieno[3,2-c]pyridine-7-carboxamide
0.036 - 0.2
4-amino-3-bromothieno[3,2-c]pyridine-7-carboxamide
0.14 - 0.2
4-amino-3-methylthieno[3,2-c]pyridine-7-carboxamide
0.025
4-amino-6-(1H-benzimidazol-2-yl)pyridazin-3(2H)-one
Escherichia coli
-
value above, pH and temperature not specified in the publication
0.000079
4-amino-8-(2,3-dihydroxypropyl)-2-[(2-methylcyclohexyl)oxy]pyrido[2,3-d]pyrimidin-5(8H)-one
Escherichia coli
-
pH and temperature not specified in the publication
0.000057
4-amino-8-(2-hydroxyethyl)-2-(2-methylcyclohexyl)pyrido[2,3-d]pyrimidin-5(8H)-one
Escherichia coli
-
pH and temperature not specified in the publication
0.00042
4-amino-8-(2-hydroxyethyl)-2-(4-methylcyclohexyl)pyrido[2,3-d]pyrimidin-5(8H)-one
Escherichia coli
-
pH and temperature not specified in the publication
0.00106
4-amino-8-(2-hydroxyethyl)-2-(propan-2-yloxy)pyrido[2,3-d]pyrimidin-5(8H)-one
Escherichia coli
-
pH and temperature not specified in the publication
0.000028
4-amino-8-(3-fluoro-2-hydroxypropyl)-2-[(2-methylcyclohexyl)oxy]pyrido[2,3-d]pyrimidin-5(8H)-one
Escherichia coli
-
pH and temperature not specified in the publication
0.00002
4-amino-8-(cyclopent-3-en-1-ylmethyl)-2-[(2-methylcyclohexyl)oxy]pyrido[2,3-d]pyrimidin-5(8H)-one
Escherichia coli
-
pH and temperature not specified in the publication
0.00035
4-amino-8-[(1R)-2-hydroxycyclopentyl]-2-[[4-(trifluoromethyl)cyclohexyl]oxy]pyrido[2,3-d]pyrimidin-5(8H)-one
Escherichia coli
-
pH and temperature not specified in the publication
0.000225
4-amino-8-[(1S)-2,3-dihydroxycyclopentyl]-2-[(4-methylcyclohexyl)oxy]pyrido[2,3-d]pyrimidin-5(8H)-one
Escherichia coli
-
pH and temperature not specified in the publication
0.000039
4-amino-8-[(1S,2S)-2-hydroxycyclopentyl]-2-[(4-methylcyclohexyl)oxy]pyrido[2,3-d]pyrimidin-5(8H)-one
Escherichia coli
-
pH and temperature not specified in the publication
0.00011
4-amino-8-[(2R)-2-hydroxycyclohexyl]-2-[(2-methylcyclohexyl)oxy]pyrido[2,3-d]pyrimidin-5(8H)-one
Escherichia coli
-
pH and temperature not specified in the publication
0.00019
4-amino-8-[(2R)-2-hydroxycyclopentyl]-2-[(4-methylcyclohexyl)oxy]pyrido[2,3-d]pyrimidin-5(8H)-one
Escherichia coli
-
pH and temperature not specified in the publication
0.000076
4-amino-8-[(2S,5S)-2,5-dihydroxycyclopentyl]-2-[(4-methylcyclohexyl)oxy]pyrido[2,3-d]pyrimidin-5(8H)-one
Escherichia coli
-
pH and temperature not specified in the publication
0.000068
4-amino-8-[(3,4-dihydroxycyclopentyl)methyl]-2-[(2-methylcyclohexyl)oxy]pyrido[2,3-d]pyrimidin-5(8H)-one
Escherichia coli
-
pH and temperature not specified in the publication
0.00004
4-amino-8-[[(2R)-2-hydroxycyclopentyl]methyl]-2-[(2-methylcyclohexyl)oxy]pyrido[2,3-d]pyrimidin-5(8H)-one
Escherichia coli
-
pH and temperature not specified in the publication
0.000038
4-amino-8-[[(4S)-4-hydroxytetrahydrofuran-3-yl]methyl]-2-[(2-methylcyclohexyl)oxy]pyrido[2,3-d]pyrimidin-5(8H)-one
Escherichia coli
-
pH and temperature not specified in the publication
0.075 - 0.2
4-amino-N2-ethylthieno[3,2-c]pyridine-2,7-dicarboxamide
0.0094 - 0.2
4-aminothieno[3,2-c]pyridine-2,7-dicarboxamide
0.052 - 0.2
4-aminothieno[3,2-c]pyridine-3,7-dicarboxamide
0.025 - 0.2
4-aminothieno[3,2-c]pyridine-7-carboxamide
0.00008 - 0.00012
5'-deoxy-2-(pentyloxy)adenosine
0.000038 - 0.00018
5'-deoxy-2-(spiro[2.2]pent-1-ylmethoxy)adenosine
0.00014 - 0.00267
5'-deoxy-2-phenoxyadenosine
0.00005 - 0.00016
5'-deoxy-2-[(trans-4-methylcyclohexyl)oxy]adenosine
0.00012
5'-deoxy-5'-fluoro-2-(pentyloxy)adenosine
0.00001 - 0.012
5'-deoxy-5'-fluoro-2-[(trans-4-methylcyclohexyl)oxy]adenosine
0.00037 - 0.0004
5'-deoxy-5'-fluoro-2-[[1-(hydroxymethyl)cyclopropyl]methoxy]adenosine
0.00049
5-([4-amino-2-[(4-methylcyclohexyl)oxy]-5-oxopyrido[2,3-d]pyrimidin-8(5H)-yl]methyl)thiophene-2-carboxylic acid
Escherichia coli
-
pH and temperature not specified in the publication
0.00965
5-[[[(4-chlorophenyl)amino]carbonyl](phenylmethyl)amino]-5,6-dideoxy-1,2-O-(1-methylethylidene)-3-O-(phenylmethyl)-alpha-L-gluco-heptofuranuronic acid ethyl ester
Mycobacterium tuberculosis
-
IC50: 0.00965 mM
0.025
7-methoxy-6-methylpteridine-2,4-diamine
Escherichia coli
-
pH and temperature not specified in the publication
0.00004
9-(3-chloro-3,5-dideoxy-beta-D-xylofuranosyl)-2-(cyclopentyloxy)-9H-purin-6-amine
0.00002 - 0.00003
9-(3-chloro-3,5-dideoxy-beta-D-xylofuranosyl)-2-(spiro[2.2]pent-1-ylmethoxy)-9H-purin-6-amine
0.00008 - 0.00011
9-(3-chloro-3-deoxy-beta-D-xylofuranosyl)-2-(cyclopentyloxy)-9H-purin-6-amine
0.046 - 0.053
Chloroquine
0.0014
Chloroquine diphosphate
Staphylococcus aureus
-
IC50: 0.0014 mM
0.0033
doxorubicin
Staphylococcus aureus
-
IC50: 0.0033 mM
0.00965
ethyl 3-(1-benzyl-3-(4-chlorophenyl)ureido)-3-(6-(benzyloxy)-2,2-dimethyl-dihydro-5H-furo[3,2-d][1,3]dioxol-5-yl)propanoate
Mycobacterium tuberculosis
-
-
0.004
ethyl 3-(3-benzoylthioureido)-3-(6-(benzyloxy)-2,2-dimethyl-dihydro-5H-furo[3,2-d][1,3]dioxol-5-yl)propanoate
Mycobacterium tuberculosis
-
-
0.0089 - 0.2
ethyl 4-amino-7-carbamoylthieno[3,2-c]pyridine-2-carboxylate
0.004
ethyl-3-O-benzyl-5,6-dideoxy-1,2-O-(1-methylethylidene)-5-[[(phenylcarbonyl)carbamoyl]amino]-alpha-L-xylo-heptofuranuronate
Mycobacterium tuberculosis
-
-
0.00965
ethyl-3-O-benzyl-5-[benzyl[(4-chlorophenyl)carbamoyl]amino]-5,6-dideoxy-1,2-O-(1-methylethylidene)-alpha-L-xylo-heptofuranuronate
Mycobacterium tuberculosis
-
-
0.063
hydroxychloroquine
Escherichia coli
-
-
0.0018
N,N'-bis[4-((4-(diethylamino)-1-methylbutyl)amino)-quinoline-6-yl]sebacamide
Escherichia coli
-
-
0.009
N,N'-bis[4-((4-(diethylamino)-1-methylbutyl)amino)-quinoline-8-yl]adipamide
Escherichia coli
-
-
0.0114
N,N'-bis[[(3aR,6S,6aR)-6-(benzyloxy)-2,2-dimethyltetrahydrofuro[2,3-d][1,3]dioxol-5-yl]methyl]dodecane-1,12-diamine
Mycobacterium tuberculosis
-
-
0.046
N1-(7-chloro-[4]quinolyl)-N4,N4-bis-(2-hydroxy-ethyl)-1-methyl-butanediyldiamine
0.0006
pyridochromanone
Mycobacterium tuberculosis
-
IC50: 0.0006 mM
0.0015
Quinacrine
0.005
tert-butyl 3-(7-amino-3-bromo-6-carbamoyl-1,8-naphthyridin-2-yl)azetidine-1-carboxylate
Escherichia coli
-
value above, pH and temperature not specified in the publication