Information on EC 6.3.2.33 - tetrahydrosarcinapterin synthase

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The expected taxonomic range for this enzyme is: Methanocaldococcus jannaschii

EC NUMBER
COMMENTARY
6.3.2.33
-
RECOMMENDED NAME
GeneOntology No.
tetrahydrosarcinapterin synthase
-
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
ATP + tetrahydromethanopterin + L-glutamate = ADP + phosphate + 5,6,7,8-tetrahydrosarcinapterin
show the reaction diagram
-
-
-
-
GTP + tetrahydromethanopterin + L-glutamate = GDP + phosphate + 5,6,7,8-tetrahydrosarcinapterin
show the reaction diagram
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Folate biosynthesis
-
-
methanogenesis from CO2
-
-
SYSTEMATIC NAME
IUBMB Comments
tetrahydromethanopterin:alpha-L-glutamate ligase (ADP-forming)
This enzyme catalyses the biosynthesis of 5,6,7,8-tetrahydrosarcinapterin, a modified form of tetrahydromethanopterin found in the Methanosarcinales. It does not require K+, and does not discriminate between ATP and GTP [1].
ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ATP + tetrahydromethanopterin + L-glutamate
ADP + phosphate + 5,6,7,8-tetrahydrosarcinapterin
show the reaction diagram
Q58037
biosynthesis of the cofactor 5,6,7,8-tetrahydrosarcinapterin, the enzyme does not discriminate between ATP and GTP
-
-
?
ATP + tetrahydromethanopterin + L-glutamate
ADP + phosphate + 5,6,7,8-tetrahydrosarcinapterin
show the reaction diagram
Q58037
the enzyme does not discriminate between ATP and GTP
-
-
?
GTP + tetrahydromethanopterin + L-glutamate
GDP + phosphate + 5,6,7,8-tetrahydrosarcinapterin
show the reaction diagram
Q58037
biosynthesis of the cofactor 5,6,7,8-tetrahydrosarcinapterin, the enzyme does not discriminate between ATP and GTP
-
-
?
GTP + tetrahydromethanopterin + L-glutamate
GDP + phosphate + 5,6,7,8-tetrahydrosarcinapterin
show the reaction diagram
Q58037
the enzyme does not discriminate between ATP and GTP
-
-
?
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ATP + tetrahydromethanopterin + L-glutamate
ADP + phosphate + 5,6,7,8-tetrahydrosarcinapterin
show the reaction diagram
Q58037
biosynthesis of the cofactor 5,6,7,8-tetrahydrosarcinapterin, the enzyme does not discriminate between ATP and GTP
-
-
?
GTP + tetrahydromethanopterin + L-glutamate
GDP + phosphate + 5,6,7,8-tetrahydrosarcinapterin
show the reaction diagram
Q58037
biosynthesis of the cofactor 5,6,7,8-tetrahydrosarcinapterin, the enzyme does not discriminate between ATP and GTP
-
-
?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Mg2+
Q58037
conserved Mg-ATP-binding domain
additional information
Q58037
does not require K+ for activity
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
12
Q58037
under rate-limiting assay conditions, MptN produces 12.7 nmol of sarcinapterin per min per mg of protein in the presence of ATP and K+
12.7
Q58037
under rate-limiting assay conditions, MptN produces 12.7 nmol of sarcinapterin per min per mg of protein in the presence of GTP and K+
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
72000
Q58037
gel filtration
700925
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
homodimer
Q58037
2 * 35000, SDS-PAGE
homodimer
Q58037
2 * 33278, calculated from sequence
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
Q58037