Any feedback?
Please rate this page
(enzyme.php)
(0/150)

BRENDA support

BRENDA Home
show all | hide all No of entries

Information on EC 6.3.2.13 - UDP-N-acetylmuramoyl-L-alanyl-D-glutamate-2,6-diaminopimelate ligase and Organism(s) Pseudomonas aeruginosa

for references in articles please use BRENDA:EC6.3.2.13
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
EC Tree
IUBMB Comments
Involved in the synthesis of a cell-wall peptide in bacteria. This enzyme adds diaminopimelate in Gram-negative organisms and in some Gram-positive organisms; in others EC 6.3.2.7 (UDP-N-acetylmuramoyl-L-alanyl-D-glutamate---L-lysine ligase) adds lysine instead. It is the amino group of the L-centre of the diaminopimelate that is acylated.
Specify your search results
Select one or more organisms in this record: ?
This record set is specific for:
Pseudomonas aeruginosa
Show additional data
Do not include text mining results
Include (text mining) results
Include results (AMENDA + additional results, but less precise)
Word Map
The taxonomic range for the selected organisms is: Pseudomonas aeruginosa
The expected taxonomic range for this enzyme is: Bacteria, Archaea, Eukaryota
Synonyms
mur ligase, amide ligase, meso-diaminopimelate-adding enzyme, udp-n-acetylmuramyl tripeptide synthetase, udp-n-acetylmuramoyl-l-alanyl-d-glutamate-2,6-diaminopimelate ligase, mure synthetase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
amide ligase
-
-
Diaminopimelic-adding enzyme
-
-
-
-
mDAP ligase
-
-
-
-
Meso-diaminopimelate-adding enzyme
-
-
-
-
MurE synthetase
-
-
-
-
Synthetase, uridine diphospho-N-acetylmuramoylalanyl-D-glutamyl-meso-2,6-diaminopimelate
-
-
-
-
UDP-MurNAc-tripeptide synthetase
-
-
-
-
UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-meso-2,6-diaminopimelate synthetase
-
-
-
-
UDP-N-acetylmuramyl-tripeptide synthetase
-
-
-
-
UDPMurNAc-L-alanyl-D-glutamate:mDAP ligase (ADP-forming)
-
-
-
-
Uridine diphosphate N-acetylmuramyl-L-alanyl-D-glutamate:meso-2,6-diaminopimelate ligase
-
-
-
-
Uridine-diphosphate-N-acetylmuramyl-L-alanyl-D-glutamate:meso-2,6-diaminopimelate synthetase
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
carboxamide formation
-
-
-
-
carboxylic acid amide formation
-
-
-
-
PATHWAY SOURCE
PATHWAYS
-
-, -
SYSTEMATIC NAME
IUBMB Comments
UDP-N-acetylmuramoyl-L-alanyl-D-glutamate:(L)-meso-2,6-diaminoheptanedioate gamma-ligase (ADP-forming)
Involved in the synthesis of a cell-wall peptide in bacteria. This enzyme adds diaminopimelate in Gram-negative organisms and in some Gram-positive organisms; in others EC 6.3.2.7 (UDP-N-acetylmuramoyl-L-alanyl-D-glutamate---L-lysine ligase) adds lysine instead. It is the amino group of the L-centre of the diaminopimelate that is acylated.
CAS REGISTRY NUMBER
COMMENTARY hide
9075-09-6
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ATP + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate + meso-2,6-diaminoheptanedioate
ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-meso-2,6-diamino-heptanedioate
show the reaction diagram
-
-
-
?
UDP-N-acetylmuramoyl-L-alanyl-D-glutamate + ATP + meso-diaminopimelic acid
ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-meso-diaminopimelic acid
show the reaction diagram
-
assay at pH 8.0
-
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ATP + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate + meso-2,6-diaminoheptanedioate
ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-meso-2,6-diamino-heptanedioate
show the reaction diagram
-
-
-
?
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
MurEP1
-
time-dependent inhibition
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.14
meso-diaminopimelic acid
-
-
0.03
UDP-N-acetylmuramoyl-L-alanyl-D-glutamate
-
-
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.5 - 0.8
MurEP1
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
3.31
-
recombinant MurE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
A0A8F8R8V6_PSEAI
487
0
51194
TrEMBL
-
A0A8G5PIK6_PSEAI
487
0
51219
TrEMBL
-
A0A8G5D999_PSEAI
487
0
51205
TrEMBL
-
A0A6B1Y7G2_PSEAI
487
0
51219
TrEMBL
-
A0A8G3SIP9_PSEAI
487
0
51263
TrEMBL
-
A0A8G2PSD8_PSEAI
487
0
51221
TrEMBL
-
A0A3S0S5P4_PSEAI
487
0
51165
TrEMBL
-
A0A8G2KT54_PSEAI
487
0
51263
TrEMBL
-
A0A8G5ZYY2_PSEAI
487
0
51195
TrEMBL
-
A0A8G8FLK1_PSEAI
487
0
51165
TrEMBL
-
A0A8G3YCU6_PSEAI
487
0
51237
TrEMBL
-
A0A8G4MKP5_PSEAI
487
0
51138
TrEMBL
-
A0A0A8RHH3_PSEAI
487
0
51144
TrEMBL
-
A0A485H517_PSEAI
487
0
51192
TrEMBL
-
A0A8G5R9J6_PSEAI
487
0
51219
TrEMBL
-
A0A2R3IZ79_PSEAI
487
0
51206
TrEMBL
-
A0A8G6HMA5_PSEAI
487
0
51193
TrEMBL
-
A0A8G3ZFP4_PSEAI
487
0
51138
TrEMBL
-
A0A8G7B200_PSEAI
487
0
51231
TrEMBL
-
A0A8G2RYH2_PSEAI
487
0
51189
TrEMBL
-
Q9EY49_PSEAI
487
0
51319
TrEMBL
-
A0A6A9JU74_PSEAI
487
0
51229
TrEMBL
-
A0A8G6WRQ6_PSEAI
487
0
51223
TrEMBL
-
A0A8G4TSR2_PSEAI
487
0
51219
TrEMBL
-
A0A8G4DQ30_PSEAI
487
0
51138
TrEMBL
-
A0A8B5BII7_PSEAI
487
0
51221
TrEMBL
-
A0A241XMM9_PSEAI
487
0
51221
TrEMBL
-
A0A7M3AUI3_PSEAI
487
0
51219
TrEMBL
-
A0A072ZPH5_PSEAI
487
0
51191
TrEMBL
-
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
55000
-
SDS-PAGE
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
recombinant MurE, Talon resin
-
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
-
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Azzolina, B.A.; Yuan, X.; Anderson, M.S.; El-Sherbeini, M.
The cell wall and cell division gene cluster in the Mra operon of Pseudomonas aeruginosa: Cloning, production, and purification of active enzymes
Protein Expr. Purif.
21
393-400
2001
Pseudomonas aeruginosa
Manually annotated by BRENDA team
Paradis-Bleau, C.; Lloyd, A.; Sanschagrin, F.; Maaroufi, H.; Clarke, T.; Blewett, A.; Dowson, C.; Roper, D.I.; Bugg, T.D.; Levesque, R.C.
Pseudomonas aeruginosa MurE amide ligase: enzyme kinetics and peptide inhibitor
Biochem. J.
421
263-272
2009
Pseudomonas aeruginosa
Manually annotated by BRENDA team