Information on EC 6.3.2.13 - UDP-N-acetylmuramoyl-L-alanyl-D-glutamate-2,6-diaminopimelate ligase

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The expected taxonomic range for this enzyme is: Bacteria

EC NUMBER
COMMENTARY
6.3.2.13
-
RECOMMENDED NAME
GeneOntology No.
UDP-N-acetylmuramoyl-L-alanyl-D-glutamate-2,6-diaminopimelate ligase
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
ATP + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate + meso-2,6-diaminoheptanedioate = ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-meso-2,6-diaminoheptanedioate
show the reaction diagram
-
-
-
-
ATP + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate + meso-2,6-diaminoheptanedioate = ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-meso-2,6-diaminoheptanedioate
show the reaction diagram
acyl phosphate intermediate
-
REACTION TYPE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
carboxamide formation
-
-
-
-
carboxylic acid amide formation
-
-
-
-
PATHWAY
KEGG Link
MetaCyc Link
Lysine biosynthesis
-
Peptidoglycan biosynthesis
-
UDP-N-acetylmuramoyl-pentapeptide biosynthesis I (meso-DAP-containing)
-
SYSTEMATIC NAME
IUBMB Comments
UDP-N-acetylmuramoyl-L-alanyl-D-glutamate:(L)-meso-2,6-diaminoheptanedioate gamma-ligase (ADP-forming)
Involved with EC 6.3.2.4 (D-alanine---D-alanine ligase), EC 6.3.2.8 (UDP-N-acetylmuramate---L-alanine ligase), EC 6.3.2.9 (UDP-N-acetylmuramoyl-L-alanine---D-glutamate ligase) and EC 6.3.2.10 (UDP-N-acetylmuramoyl-tripeptide---D-alanyl-D-alanine ligase) in the synthesis of a cell-wall peptide ({polysacc/pepgly1} for diagram). This enzyme adds diaminopimelate in Gram-negative organisms and in some Gram-positive organisms; in others EC 6.3.2.7 (UDP-N-acetylmuramoyl-L-alanyl-D-glutamate---L-lysine ligase) adds lysine instead. It is the amino group of the L-centre of the diaminopimelate that is acylated.
SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
amide ligase
-
-
Diaminopimelic-adding enzyme
-
-
-
-
mDAP ligase
-
-
-
-
Meso-diaminopimelate-adding enzyme
-
-
-
-
MurE synthetase
-
-
-
-
MurE synthetase
-
-
Synthetase, uridine diphospho-N-acetylmuramoylalanyl-D-glutamyl-meso-2,6-diaminopimelate
-
-
-
-
UDP-MurNAc-tripeptide synthetase
-
-
-
-
UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-meso-2,6-diaminopimelate synthetase
-
-
-
-
UDP-N-acetylmuramyl tripeptide synthetase
-
-
UDP-N-acetylmuramyl-tripeptide synthetase
-
-
-
-
UDPMurNAc-L-alanyl-D-glutamate:mDAP ligase (ADP-forming)
-
-
-
-
Uridine diphosphate N-acetylmuramyl-L-alanyl-D-glutamate:meso-2,6-diaminopimelate ligase
-
-
-
-
Uridine-diphosphate-N-acetylmuramyl-L-alanyl-D-glutamate:meso-2,6-diaminopimelate synthetase
-
-
-
-
CAS REGISTRY NUMBER
COMMENTARY
9075-09-6
-
ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
overproduced from strain JM83(pHE5) and the very strong overproducing strain KM83(pMLD25)
-
-
Manually annotated by BRENDA team
Escherichia coli K12
K12
-
-
Manually annotated by BRENDA team
Lysinibacillus sphaericus 9602
9602
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ATP + dihydrouridine-5'-diphosphate-MurNAc-L-Ala-D-Glu + meso-2,6-diaminoheptanedioate
ADP + phosphate + dihydrouridine-5'-diphosphate-MurNAc-L-Ala-D-Glu-meso-2,6-diaminoheptanedioate
show the reaction diagram
-
-
-
-
ATP + MurNAc-L-Ala-D-Glu + meso-2,6-diaminoheptanedioate
?
show the reaction diagram
-
relative activity wild-type: 14%
-
-
?
ATP + UDP-MurNAc-L-Ala-D-Glu + D-Ala-D-Ala
?
show the reaction diagram
-
relative activity wild-type: 5%
-
-
?
ATP + UDP-MurNAc-L-Ala-D-Glu + D-allo-cystathionine
ADP + phosphate + UDP-MurNAc-L-Ala-D-Glu-D-allo-cystathionine
show the reaction diagram
-
-
-
-
ATP + UDP-MurNAc-L-Ala-D-Glu + D-Glu
?
show the reaction diagram
-
relative activity wild-type: 13%
-
-
?
ATP + UDP-MurNAc-L-Ala-D-Glu + D-Lys
?
show the reaction diagram
-
relative activity wild-type: 9%
-
-
?
ATP + UDP-MurNAc-L-Ala-D-Glu + D-ornithine
?
show the reaction diagram
-
relative activity wild-type: 8%
-
-
?
ATP + UDP-MurNAc-L-Ala-D-Glu + DL-lanthionine
?
show the reaction diagram
-
relative activity wild-type: 82%
-
-
?
ATP + UDP-MurNAc-L-Ala-D-Glu + L-Ala
?
show the reaction diagram
-
relative activity wild-type: 12%
-
-
?
ATP + UDP-MurNAc-L-Ala-D-Glu + L-allo-cystathionine
ADP + phosphate + UDP-MurNAc-L-Ala-D-Glu-L-allo-cystathionine
show the reaction diagram
-
-
-
-
ATP + UDP-MurNAc-L-Ala-D-Glu + L-cystathionine
?
show the reaction diagram
-
relative activity wild-type: 12%
-
-
?
ATP + UDP-MurNAc-L-Ala-D-Glu + L-Lys
?
show the reaction diagram
-
relative activity wild-type: 7%
-
-
?
ATP + UDP-MurNAc-L-Ala-D-Glu + meso-2,6-diaminoheptanedioate
ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-meso-2,6-diaminoheptanedioate
show the reaction diagram
-
-
-
-
-
ATP + UDP-MurNAc-L-Ala-D-Glu + meso-2,6-diaminoheptanedioate
ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-meso-2,6-diaminoheptanedioate
show the reaction diagram
-
-
-
-
-
ATP + UDP-MurNAc-L-Ala-D-Glu + meso-2,6-diaminoheptanedioate
ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-meso-2,6-diaminoheptanedioate
show the reaction diagram
-
-
-
-
-
ATP + UDP-MurNAc-L-Ala-D-Glu + meso-2,6-diaminoheptanedioate
ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-meso-2,6-diaminoheptanedioate
show the reaction diagram
-
r
-
-
-
ATP + UDP-MurNAc-L-Ala-D-Glu + meso-2,6-diaminoheptanedioate
ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-meso-2,6-diaminoheptanedioate
show the reaction diagram
-
r
-
-
-
ATP + UDP-MurNAc-L-Ala-D-Glu + meso-2,6-diaminoheptanedioate
ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-meso-2,6-diaminoheptanedioate
show the reaction diagram
-
specific for UDP-N-MurNAc-L-Ala-D-Glu and meso-2,6-diaminoheptanedioate
-
-
-
ATP + UDP-MurNAc-L-Ala-D-Glu + meso-2,6-diaminoheptanedioate
ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-meso-2,6-diaminoheptanedioate
show the reaction diagram
-
relative activity wild-type: 100%
-
-
?
ATP + UDP-MurNAc-L-Ala-D-Glu + meso-2,6-diaminoheptanedioate
ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-meso-2,6-diaminoheptanedioate
show the reaction diagram
-, O69557
the threedimensional structure of MurE from Mycobacterium leprae is modeled using comparative modeling methods based on the X-ray crystal structure of MurE from Escherichia coli. The docked complexes reveal the amino acids responsible for binding the substrates. Superposition of these complex structures suggests that carboxylic acid group of UDP-Nacetyl muramoyl-glycyl-D-glutamate is positioned in proximity to gamma-phosphate of the ATP to facilitate the formation of acylphosphate intermediate. The orientation of an amino group of meso-diaminopimelic acid facilitates the nucleophilic attack to form the product
-
-
?
ATP + UDP-MurNAc-L-Ala-D-Glu + meso-2,6-diaminoheptanedioate
ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-meso-2,6-diaminoheptanedioate
show the reaction diagram
Bacillus subtilis 168
-
-
-
-
-
ATP + UDP-MurNAc-L-Ala-D-Glu + meso-2,6-diaminoheptanedioate
ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-meso-2,6-diaminoheptanedioate
show the reaction diagram
Lysinibacillus sphaericus 9602
-
-
-
-
-
ATP + UDP-MurNAc-L-Ala-D-Glu + meso-2,6-diaminoheptanedioate
ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-meso-2,6-diaminoheptanedioate
show the reaction diagram
Escherichia coli K12
-
-
-
-
-
ATP + UDP-MurNAc-L-Ala-D-Glu + meso-lanthionine
ADP + phosphate + UDP-MurNAc-L-Ala-D-Glu-meso-lanthionine
show the reaction diagram
-
-
-
-
ATP + UDP-N-acetylmuramoyl-L-Ala-D-Glu + meso-diaminopimelic acid
ADP + phosphate + UDP-N-acetylmuramoyl-L-Ala-D-Glu-meso-diaminopimelic acid
show the reaction diagram
P65477
-
-
-
?
ATP + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate + D-lysine
ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-D-lysine
show the reaction diagram
-
-
-
-
?
ATP + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate + L-lysine
ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-L-lysine
show the reaction diagram
-
-
-
-
?
ATP + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate + L-lysine
ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-L-lysine
show the reaction diagram
-
-
-
-
?
ATP + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate + L-ornithine
ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-L-ornithine
show the reaction diagram
-
-
-
-
?
ATP + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate + meso-2,6-diaminoheptanedioate
ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-meso-2,6-diamino-heptanedioate
show the reaction diagram
-
-
-
?
ATP + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate + meso-2,6-diaminoheptanedioate
ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-meso-2,6-diamino-heptanedioate
show the reaction diagram
-
-
-
?
ATP + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate + meso-2,6-diaminoheptanedioate
ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-meso-2,6-diamino-heptanedioate
show the reaction diagram
-
-
-
-
?
ATP + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate + meso-2,6-diaminoheptanedioate
ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-meso-2,6-diamino-heptanedioate
show the reaction diagram
P22188
-
-
r
ATP + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate + meso-2,6-diaminoheptanedioate
ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-meso-2,6-diamino-heptanedioate
show the reaction diagram
-
-
-
?
ATP + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate + meso-2,6-diaminoheptanedioate
ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-meso-2,6-diamino-heptanedioate
show the reaction diagram
-
essential for optimal expression of methicillin resistance
-
?
ATP + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate + meso-2,6-diaminoheptanedioate
ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-meso-2,6-diamino-heptanedioate
show the reaction diagram
P22188
part of bacterial cell-wall peptidoglycan biosynthesis
-
r
ATP + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate + meso-diaminopimelic acid
ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-meso-diaminopimelic acid
show the reaction diagram
-
-
-
-
?
ATP + UDP-N-MurNAc-L-Ala-D-Glu + meso-2,6-diaminoheptanedioate
?
show the reaction diagram
-
enzyme activity is required exclusively for cortical peptidoglycan synthesis, it is absent during vegetative growth, and is synthesized during forespore maturation
-
-
-
ATP + UDP-N-MurNAc-L-Ala-D-Glu + meso-2,6-diaminoheptanedioate
?
show the reaction diagram
-
catalyzes an early reaction in the biosynthesis of bacterial peptidoglycan
-
-
-
ATP + UDP-N-MurNAc-L-Ala-D-Glu + meso-2,6-diaminoheptanedioate
?
show the reaction diagram
-
the adding activity is not in excess in the cell under normal growth conditions, but their amounts appear adjusted to the requirements of peptidoglycan synthesis
-
-
-
ATP + UDP-N-MurNAc-L-Ala-D-Glu + meso-2,6-diaminoheptanedioate
?
show the reaction diagram
-
enzyme is not essential for growth
-
-
-
ATP + UDP-N-MurNAc-L-Ala-D-Glu + meso-2,6-diaminoheptanedioate
?
show the reaction diagram
Lysinibacillus sphaericus 9602
-
enzyme activity is required exclusively for cortical peptidoglycan synthesis, it is absent during vegetative growth, and is synthesized during forespore maturation
-
-
-
ATP + UDP-N-MurNAc-L-Ala-D-Glu + meso-2,6-diaminoheptanedioate
?
show the reaction diagram
Escherichia coli K12
-
the adding activity is not in excess in the cell under normal growth conditions, but their amounts appear adjusted to the requirements of peptidoglycan synthesis
-
-
-
UDP-N-acetylmuramoyl-L-alanyl-D-glutamate + ATP + meso-diaminopimelic acid
ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-meso-diaminopimelic acid
show the reaction diagram
-
-
-
-
?
UDP-N-acetylmuramoyl-L-alanyl-D-glutamate + ATP + meso-diaminopimelic acid
ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-meso-diaminopimelic acid
show the reaction diagram
-
assay at pH 8.0
-
-
?
UTP + UDP-MurNAc-L-Ala-D-Glu + meso-2,6-diaminoheptanedioate
UDP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-meso-2,6-diaminoheptanedioate
show the reaction diagram
-
-
-
-
-
UTP + UDP-MurNAc-L-Ala-D-Glu + meso-2,6-diaminoheptanedioate
UDP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-meso-2,6-diaminoheptanedioate
show the reaction diagram
-
16% of the activity relative to ATP
-
-
UTP + UDP-MurNAc-L-Ala-D-Glu + meso-2,6-diaminoheptanedioate
UDP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-meso-2,6-diaminoheptanedioate
show the reaction diagram
-
relative activity wild-type: 17%
-
-
?
GTP + UDP-MurNAc-L-Ala-D-Glu + meso-2,6-diaminoheptanedioate
GDP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-meso-2,6-diaminoheptanedioate
show the reaction diagram
-
relative activity wild-type: 30%
-
-
?
additional information
?
-
P65477
highly specific in adding meso-diaminopimelic acid to UDP-MurNAc-dipeptide. No substrate: L-Lys, D-Lys, m-Lys, L-Ala, D-Ala, L-Glu, D-Glu, Gly, Gly-Gly, DL-ornithine, N-acetylmuramic acid, UDP-N-acetylmuramoyl pentapeptide. ATP-hydrolysis is an absolute requirement for activity
-
-
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ATP + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate + meso-2,6-diaminoheptanedioate
ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-meso-2,6-diamino-heptanedioate
show the reaction diagram
-
-
-
?
ATP + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate + meso-2,6-diaminoheptanedioate
ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-meso-2,6-diamino-heptanedioate
show the reaction diagram
-
-
-
?
ATP + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate + meso-2,6-diaminoheptanedioate
ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-meso-2,6-diamino-heptanedioate
show the reaction diagram
-
essential for optimal expression of methicillin resistance
-
?
ATP + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate + meso-2,6-diaminoheptanedioate
ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-meso-2,6-diamino-heptanedioate
show the reaction diagram
P22188
part of bacterial cell-wall peptidoglycan biosynthesis
-
r
ATP + UDP-N-MurNAc-L-Ala-D-Glu + meso-2,6-diaminoheptanedioate
?
show the reaction diagram
-
enzyme activity is required exclusively for cortical peptidoglycan synthesis, it is absent during vegetative growth, and is synthesized during forespore maturation
-
-
-
ATP + UDP-N-MurNAc-L-Ala-D-Glu + meso-2,6-diaminoheptanedioate
?
show the reaction diagram
-
catalyzes an early reaction in the biosynthesis of bacterial peptidoglycan
-
-
-
ATP + UDP-N-MurNAc-L-Ala-D-Glu + meso-2,6-diaminoheptanedioate
?
show the reaction diagram
-
the adding activity is not in excess in the cell under normal growth conditions, but their amounts appear adjusted to the requirements of peptidoglycan synthesis
-
-
-
ATP + UDP-N-MurNAc-L-Ala-D-Glu + meso-2,6-diaminoheptanedioate
?
show the reaction diagram
-
enzyme is not essential for growth
-
-
-
ATP + UDP-N-MurNAc-L-Ala-D-Glu + meso-2,6-diaminoheptanedioate
?
show the reaction diagram
Lysinibacillus sphaericus 9602
-
enzyme activity is required exclusively for cortical peptidoglycan synthesis, it is absent during vegetative growth, and is synthesized during forespore maturation
-
-
-
ATP + UDP-N-MurNAc-L-Ala-D-Glu + meso-2,6-diaminoheptanedioate
?
show the reaction diagram
Escherichia coli K12
-
the adding activity is not in excess in the cell under normal growth conditions, but their amounts appear adjusted to the requirements of peptidoglycan synthesis
-
-
-
METALS and IONS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
Cd2+
-
can partially substitute for Mg2+
Co2+
-
can partially substitute for Mg2+
K+
-
K+ or NH4+ required
K+
-
potassium phosphate increases activity
Mg2+
-
Mg2+ or Mn2+ required; optimal concentration of Mg2+ is 10 mM
Mg2+
-
optimal concentration: 1-13 mM in endospores, 3-10 mM in vegetative cells
Mg2+
-
15 mM for optimal activity
Mn2+
-
Mg2+ or Mn2+ required. With Mn2+ maximal activity is found at concentrations to half that of ATP
NH4+
-
K+ or NH4+ required
Zn2+
-
can partially substitute for Mg2+
INHIBITORS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
1-methyl-2-[(4Z)-tetradec-4-en-1-yl]quinolin-4(1H)-one
-
inhibitory to enzyme and growth inhibitor of Mycobacterium tuberculosis and rapid-growing mycobacteria
1-methyl-2-[(5Z)-tetradec-5-en-1-yl]quinolin-4(1H)-one
-
inhibitory to enzyme and growth inhibitor of Mycobacterium tuberculosis and rapid-growing mycobacteria
2-[(1E)-dec-1-en-1-yl]-1-methylquinolin-4(1H)-one
-
inhibitory to enzyme and growth inhibitor of Mycobacterium tuberculosis and rapid-growing mycobacteria
2-[(1E)-dodec-1-en-1-yl]-1-methylquinolin-4(1H)-one
-
inhibitory to enzyme and growth inhibitor of Mycobacterium tuberculosis and rapid-growing mycobacteria
2-[(1E)-tridec-1-en-1-yl]-1-methylquinolin-4(1H)-one
-
inhibitory to enzyme and growth inhibitor of Mycobacterium tuberculosis and rapid-growing mycobacteria
Butylamine
-
500 mM, 88% inhibition
Butyrate
-
500 mM, 44% inhibition
ethylamine
-
500 mM, 73% inhibition
MurEP1
-
time-dependent inhibition
-
MurNAc(alpha-methyl)-L-Ala-D-Glu
-
-
MurNAc(beta-methyl)-L-Ala-D-Glu
-
-
N-([(2S)-2-[(2-naphthylsulfonyl)amino]propyl]-sulfonyl)-D-glutamic acid
-
60% residual activity at 1 mM
N-([(2S)-2-[([1,1'-biphenyl]-4-yl-sulfonyl)amino]-propyl]sulfonyl)-D-glutamic acid
-
12% residual activity at 1 mM
N-[((2S)-2-[[(E)-3-(1,3-benzodioxol-5-yl)-2-propenoyl]amino]propyl)sulfonyl]-D-glutamic acid
-
64% residual activity at 1 mM
N-[[(2S)-2-(1,3-dioxo-1,3-dihydro-2H-isoindol-2-yl)propyl]sulfonyl]-D-glutamic acid
-
41% residual activity at 1 mM
N-[[(2S)-2-([2-[2-(acetylamino)phenoxy]acetyl]-amino)propyl]sulfonyl]-D-glutamic acid
-
56% residual activity at 1 mM
N-[[2-(1,3-dioxo-1,3-dihydro-2H-isoindol-2-yl)-ethyl]sulfonyl]-D-glutamic acid
-
1 mM
Nalpha-Propionyl-L-Ala-2-amino-6-phosphonohexanoate
-
-
Nalpha-Propionyl-L-Ala-3-phosphonoacetamido-Ala
-
-
Nalpha-Propionyl-L-Ala-buthionine sulfoximine
-
-
P1-MurNAc-L-Ala-D-Glu
-
-
Propionyl-L-Ala-D-Glu(CH2Cl)-OH
-
-
Propionyl-L-Ala-xi-2-amino-4-phosphonobutanoate
-
-
Propylamine
-
500 mM, 87% inhibition
UDP-MurNAc-L-Ala-D-Glu
-
above 0.4 mM
Methylamine
-
500 mM, 80% inhibition
additional information
-
-
-
KM VALUE [mM]
KM VALUE [mM] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.008
-
ATP
-
mutant K157A, pH 8.5, 37C
0.012
-
ATP
P65477
pH 7.4, 37C
0.015
-
ATP
-
mutant R451A, pH 8.5, 37C
0.017
-
ATP
-
wild-type, pH 8.5, 37C
0.021
-
ATP
-
DELTA1-24 (N-terminal deletion mutant), pH 8.5, 37C
0.06
-
ATP
-
mutant N449D, pH 8.5, 37C
0.1
-
ATP
-
mutant D392A, pH 8.5, 37C
0.123
-
ATP
-
mutant E220A, pH 8.5, 37C
0.62
-
ATP
-
-
3.6
-
ATP
-
at 68C
10
-
D-allo-cystathionine
-
-
1.7
-
D-Lysine
-
at 68C
0.097
-
dihydrouridine-5'-diphosphate-MurNAc-L-Ala-D-Glu
-
-
3.9
-
L-allo-cystathionine
-
-
2.8
-
L-lysine
-
at 68C
27
-
L-ornithine
-
at 68C
0.012
-
meso-2,6-diaminoheptanedioate
-
mutant K157A, pH 8.5, 37C
0.036
-
meso-2,6-diaminoheptanedioate
-
-
0.04
-
meso-2,6-diaminoheptanedioate
-
-
0.052
-
meso-2,6-diaminoheptanedioate
-
mutant E220A, pH 8.5, 37C
0.065
-
meso-2,6-diaminoheptanedioate
-
DELTA1-24 (N-terminal deletion mutant), pH 8.5, 37C
0.078
-
meso-2,6-diaminoheptanedioate
-
wild-type, pH 8.5, 37C
0.113
-
meso-2,6-diaminoheptanedioate
-
mutant D392A, pH 8.5, 37C
0.13
-
meso-2,6-diaminoheptanedioate
-
-
0.324
-
meso-2,6-diaminoheptanedioate
-
mutant N449D, pH 8.5, 37C
0.473
-
meso-2,6-diaminoheptanedioate
-
mutant R451A, pH 8.5, 37C
0.069
-
meso-diaminopimelic acid
P65477
pH 7.4, 37C
0.14
-
meso-diaminopimelic acid
-
-
4.8
-
meso-diaminopimelic acid
-
at 68C
1.5
-
meso-lanthionine
-
-
0.005
-
UDP-MurNAc-L-Ala-D-Glu
-
mutant K157A, pH 8.5, 37C, value below; mutant R451A, pH 8.5, 37C
0.007
-
UDP-MurNAc-L-Ala-D-Glu
-
mutant N449D, pH 8.5, 37C
0.016
-
UDP-MurNAc-L-Ala-D-Glu
-
mutant D392A, pH 8.5, 37C
0.019
-
UDP-MurNAc-L-Ala-D-Glu
-
DELTA1-24 (N-terminal deletion mutant), pH 8.5, 37C
0.024
-
UDP-MurNAc-L-Ala-D-Glu
-
wild-type, pH 8.5, 37C
0.148
-
UDP-MurNAc-L-Ala-D-Glu
-
mutant E220A, pH 8.5, 37C
0.04
-
UDP-N-acetylmuramoyl-L-Ala-D-Glu
P65477
pH 7.4, 37C
0.03
-
UDP-N-acetylmuramoyl-L-alanyl-D-glutamate
-
-
0.45
-
UDP-N-acetylmuramoyl-L-alanyl-D-glutamate
-
at 68C
0.035
-
UDP-N-MurNAc-L-Ala-D-Glu
-
-
-
0.076
-
UDP-N-MurNAc-L-Ala-D-Glu
-
-
-
0.32
-
UDP-N-MurNAc-L-Ala-D-Glu
-
-
-
TURNOVER NUMBER [1/s]
TURNOVER NUMBER MAXIMUM[1/s]
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.05
-
ATP
-
mutant N449D, pH 8.5, 37C, co-substrate: UDP-MurNAc-L-Ala-D-Glu, meso-2,6-diaminoheptanedioate
0.06
-
ATP
-
mutant R451A, pH 8.5, 37C, co-substrate: UDP-MurNAc-L-Ala-D-Glu, meso-2,6-diaminoheptanedioate
0.08
-
ATP
-
mutant K157A, pH 8.5, 37C, co-substrate: UDP-MurNAc-L-Ala-D-Glu, meso-2,6-diaminoheptanedioate
0.1
-
ATP
-
mutant D392A, pH 8.5, 37C, co-substrate: UDP-MurNAc-L-Ala-D-Glu, meso-2,6-diaminoheptanedioate
0.56
-
ATP
-
mutant E220A, pH 8.5, 37C, co-substrate: UDP-MurNAc-L-Ala-D-Glu, meso-2,6-diaminoheptanedioate
1.11
-
ATP
-
N-terminal deletion mutant, pH 8.5, 37C, co-substrate: UDP-MurNAc-L-Ala-D-Glu, meso-2,6-diaminoheptanedioate
1.3
-
ATP
-
wild-type, pH 8.5, 37C, co-substrate: UDP-MurNAc-L-Ala-D-Glu, meso-2,6-diaminoheptanedioate
IC50 VALUE [mM]
IC50 VALUE [mM] Maximum
INHIBITOR
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.052
-
1-methyl-2-[(4Z)-tetradec-4-en-1-yl]quinolin-4(1H)-one
-
phosphate-based method, pH 8.5, 37C
0.159
-
1-methyl-2-[(4Z)-tetradec-4-en-1-yl]quinolin-4(1H)-one
-
HPLC method, pH 8.5, 37C
0.036
-
1-methyl-2-[(5Z)-tetradec-5-en-1-yl]quinolin-4(1H)-one
-
phosphate-based method, pH 8.5, 37C
0.095
-
1-methyl-2-[(5Z)-tetradec-5-en-1-yl]quinolin-4(1H)-one
-
HPLC method, pH 8.5, 37C
0.07
-
2-[(1E)-dec-1-en-1-yl]-1-methylquinolin-4(1H)-one
-
phosphate-based method, pH 8.5, 37C
0.207
-
2-[(1E)-dec-1-en-1-yl]-1-methylquinolin-4(1H)-one
-
HPLC method, pH 8.5, 37C
0.072
-
2-[(1E)-dodec-1-en-1-yl]-1-methylquinolin-4(1H)-one
-
phosphate-based method, pH 8.5, 37C
0.187
-
2-[(1E)-dodec-1-en-1-yl]-1-methylquinolin-4(1H)-one
-
HPLC method, pH 8.5, 37C
0.052
-
2-[(1E)-tridec-1-en-1-yl]-1-methylquinolin-4(1H)-one
-
phosphate-based method, pH 8.5, 37C
0.14
-
2-[(1E)-tridec-1-en-1-yl]-1-methylquinolin-4(1H)-one
-
HPLC method, pH 8.5, 37C
0.5
-
MurEP1
-
30 min pre-incupation
-
0.8
-
MurEP1
-
10 min pre-incubation
-
SPECIFIC ACTIVITY [µmol/min/mg]
SPECIFIC ACTIVITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
0.00259
-
-
-
0.0047
-
-
K224A mutant
0.0393
-
-
-
0.078
-
-
K224A mutant, in the presence of 500 mM propionate
0.625
-
P22188
recombinant His-tagged MurE
1.24
-
-
recombinant wild-type MurE
3.31
-
-
recombinant MurE
pH OPTIMUM
pH MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
8.3
9
-
-
8.3
-
-
-
8.5
9.2
-
vegetative cells
8.8
9.9
-
endospores
pH RANGE
pH RANGE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
7.2
9.5
-
about 40% of maximal activity at pH 7.2 and 9.5
TEMPERATURE OPTIMUM
TEMPERATURE OPTIMUM MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
TEMPERATURE RANGE
TEMPERATURE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
37
-
-
the enzyme has 25% of its maximum activity at 37C
SOURCE TISSUE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
SOURCE
-
,enzyme activity is required exclusively for cortical peptidoglycan synthesis, it is absent during vegetative growth, and is synthesized during forespore maturation
Manually annotated by BRENDA team
Lysinibacillus sphaericus 9602
-
,enzyme activity is required exclusively for cortical peptidoglycan synthesis, it is absent during vegetative growth, and is synthesized during forespore maturation
-
Manually annotated by BRENDA team
-
the enzyme is absent from cells harvested before 4 h of sporulation
Manually annotated by BRENDA team
Lysinibacillus sphaericus 9602
-
the enzyme is absent from cells harvested before 4 h of sporulation
-
Manually annotated by BRENDA team
Bacillus subtilis 168
-
-
-
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
Lysinibacillus sphaericus 9602
-
-
-
Manually annotated by BRENDA team
Bacillus subtilis 168
-
endospore
-
-
Manually annotated by BRENDA team
-
vegetative cells
-
Manually annotated by BRENDA team
Bacillus subtilis 168
-
vegetative cells
-
-
Manually annotated by BRENDA team
PDB
SCOP
CATH
ORGANISM
Escherichia coli (strain K12)
MOLECULAR WEIGHT
MOLECULAR WEIGHT MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
55000
-
-
SDS-PAGE
56000
-
-
SDS-PAGE
62000
-
-
gel filtration
SUBUNITS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
monomer
-
1 * 56000, SDS-PAGE
Crystallization/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
crystals of native and selenomethionine MurE are grown in the presence of UDP-N-acetylmuramoyl-L-alanyl-D-glutamate, 0.002 ml of protein solution containing 10 mg/ml enzyme, 20 mM HEPES, pH 7.5 200 mM NaCl, 5 mM dithiothreitol and 1 mM UDP-N-acetylmuramoyl-L-alanyl-D-glutamate, are equilibrated together with 0.002 ml reservoir buffer consisting of 100 mM HEPES, pH 7.5, 13% polyethylene glycol monomethyl ether 5000, 500 mM LiCl, 10% isopropanol and 5 mM dithiothreitol, against 1 ml reservoir buffer, crystals diffract to 2.0 A
P22188
modeling of the threedimensional structure using comparative modeling methods based on the X-ray crystal structure of MurE from Escherichia coli. The 3D-structure was docked with its substrates meso-diaminopimelic acid and UDP-N-acetylmuramoyl-glycyl-D-glutamate and its product UDP-N-acetyl muramoyl-glycyl-D-Glu-meso-diaminopimelate and also with ATP. The carboxylic acid group of UDP-N-acetylmuramoyl-glycyl-D-glutamate is positioned in proximity to gamma-phosphate of the ATP to facilitate the formation of acylphosphate intermediate. The orientation of an amino group of diaminopimelate facilitates the nucleophilic attack to form the product
-, O69557
computational docking of inhibitors to crystal strucuture. Uracil recognition site is a probable binding site for quinolone inhibitors
-
to 3.0 A resolution in the presence of the substrate UDP-MurNAc-L-Ala-D-Glu. Protein consists of 3 domains consisting of residues 25-139,140-378 and 379-535, respectively. The UAG ligand binds to both domains 1 and 2 with the UDP portion binding to domain 1 and the peptide to domain 2. The substrate molecule UAG is bound between domains 1 and 2
P65477
STORAGE STABILITY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
-18C, retains more than 80% of the activity after 3 months
-
-20C, complete loss of activity after 2 d
-
Purification/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
recombinant His-tagged MurE, Ni2+-nitrilotriacetate agarose
P22188
recombinant MurE
-
using Ni-NTA chromatography
-
recombinant MurE, Talon resin
-
Ni2+-nitrilotriacetate-agarose chromatography
-
Cloned/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
expresision of His-tagged MurE in Escherichia coli
-
expression of His-tagged MurE and selenomethionine-MurE in Escherichia coli
P22188
expressed in Escherichia coli as a His-tagged fusion protein
-
expression in Escherichia coli
P65477
expression in Escherichia coli
-
expressed in Escherichia coli strains BL21(DE3)/pLysS and DH5alpha
-
ENGINEERING
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
D392A
-
kcat lower than wild-type, Km (meso-2,6-diaminoheptanedioate) and Km (ATP) higher than wild-type, Km (UDP-MurNAc-L-Ala-D-Glu) lower than wild-type, mutant shows hydrolysis of ATP uncoupled from catalysis. The ATP hydrolysis rate is enhanced by at least partial occupation of the uridine nucleotide dipeptide binding site, mutant shows very high activity with all the amino acids, uridine sugar precursors and nucleotides tested compared to wild type and moreover, reaching up to 100%
DELTA1-24
-
N-terminal deletion mutant, kcat comparable to wild-type, Km values comparable to wild-type, mutant shows similar results to the wild-type with all the nucleotides, UDP-MurNAc peptides and amino acids
E220A
-
kcat lower than wild-type, Km (ATP) and Km (UDP-MurNAc-L-Ala-D-Glu), Km (meso-2,6-diaminoheptanedioate) lower than wild-type, mutant shows hydrolysis of ATP uncoupled from catalysis. The ATP hydrolysis rate is enhanced by atleast partial occupation of the uridine nucleotide dipeptide binding site, mutant shows very high activity with all the amino acids, uridine sugar precursors and nucleotides tested compared to wild type and moreover, reaching up to 100%
K157A
-
kcat lower than wild-type, Km (meso-2,6-diaminoheptanedioate), Km (UDP-MurNAc-L-Ala-D-Glu) and Km (ATP) lower than wild-type, mutant shows hydrolysis of ATP uncoupled from catalysis. The ATP hydrolysis rate is enhanced by at least partial occupation of the uridine nucleotide dipeptide binding site, mutant shows very high activity with all the amino acids, uridine sugar precursors and nucleotides tested compared to wild type and moreover, reaching up to 100%
N449D
-
kcat lower than wild-type, Km (ATP) and Km (meso-2,6-diaminoheptanedioate) higher than wild-type, Km (UDP-MurNAc-L-Ala-D-Glu) lower than wild-type, mutant shows similar results to the wild-type with all the nucleotides, UDP-MurNAc peptides and amino acids, except D-Lys, which shows a slightly higher activity for mutant N449D
R451A
-
kcat lower than wild-type, Km (ATP) comparable to wild-type, Km (meso-2,6-diaminoheptanedioate) higher than wild-type, Km (UDP-MurNAc-L-Ala-D-Glu) lower than wild-type, significant activity in the presence of L-Lys, D-Lys, DL-ornithine and D-Glu for mutant R451A with similar activities as wild type for all the nucleotides and UDP-MurNAc peptides tested