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The expected taxonomic range for this enzyme is: Arachis hypogaea
Synonyms
4-methyleneglutamine synthetase,
more
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4-Methyleneglutamine synthetase
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4-Methylenenglutamate:ammonia ligase (AMP-forming)
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Synthetase, methyleneglutamine
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ATP + 4-methylene-L-glutamate + NH3 = AMP + diphosphate + 4-methylene-L-glutamine
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Acid amide hydrolysis
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carboxylic
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4-methylene-L-glutamate:ammonia ligase (AMP-forming)
Glutamine can act instead of NH3, but more slowly.
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ATP + 4-methylene-L-glutamate + glutamine
AMP + diphosphate + glutamate + 4-methylene-L-glutamine
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about 20% as effective as NH4+
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ATP + 4-methylene-L-glutamate + hydroxylamine
AMP + diphosphate + 4-methylene-L-glutamylhydroxamate
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70% as effective as NH4+
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ATP + 4-methylene-L-glutamate + NH3
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enzyme is responsible for the rapid synthesis of 4-methyleneglutamine which occurs in germinating peanuts
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ATP + 4-methylene-L-glutamate + NH4+
AMP + diphosphate + 4-methylene-L-glutamine
additional information
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no amide/hydroxylamine exchange
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ATP + 4-methylene-L-glutamate + NH4+
AMP + diphosphate + 4-methylene-L-glutamine
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ATP + 4-methylene-L-glutamate + NH4+
AMP + diphosphate + 4-methylene-L-glutamine
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ATP + 4-methylene-L-glutamate + NH3
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enzyme is responsible for the rapid synthesis of 4-methyleneglutamine which occurs in germinating peanuts
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Asp
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with NH4+ is nitrogen donor
diphosphate
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in presence of F-
Glu
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with NH4+ is nitrogen donor
methionine sulfoximine
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partial
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2.7 - 8
4-methylene-L-glutamate
2.7
4-methylene-L-glutamate
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8
4-methylene-L-glutamate
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about
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6.6 - 8.1
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more than 70% of maximal activity at pH 6.6 and 8.1
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brenda
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brenda
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226000
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sedimentation velocity centrifugation
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Winter, H.C.; Ti-Zhi Su; Dekker, E.E.
4-Methyleneglutamine synthetase: a new amide synthetase present in germinating peanuts
Biochem. Biophys. Res. Commun.
111
484-489
1983
Arachis hypogaea
brenda
Winter, H.C.; Dekker, E.E.
Purification and characterization of a novel 4-methyleneglutamine synthetase from germinating peanut cotyledons (Arachis hypogaea)
J. Biol. Chem.
261
11189-11193
1986
Arachis hypogaea
brenda
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