Information on EC 6.3.1.6 - glutamate-ethylamine ligase

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The expected taxonomic range for this enzyme is: Camellia sinensis

EC NUMBER
COMMENTARY
6.3.1.6
-
RECOMMENDED NAME
GeneOntology No.
glutamate-ethylamine ligase
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
ATP + L-glutamate + ethylamine = ADP + phosphate + N5-ethyl-L-glutamine
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Acid amide hydrolysis
-
-
carboxylic
-
SYSTEMATIC NAME
IUBMB Comments
L-glutamate:ethylamine ligase (ADP-forming)
-
SYNONYMS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
N5-Ethyl-L-glutamine synthetase
-
-
-
-
Synthetase, N5-ethylglutamine
-
-
-
-
Theanine synthetase
-
-
-
-
Theanine synthetase
-
-
CAS REGISTRY NUMBER
COMMENTARY
62213-31-4
-
ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
DD410895
GenBank
Manually annotated by BRENDA team
Anhui Agricultural University, Hefei city, Anhui Province, China
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
metabolism
-
the enzyme is involved in theanine biosynthesis in tea plants. Theanine is the most abundant amino acid in tea seedlings
metabolism
DD410895
theanine synthetase is a key enzyme involved in theanine biosynthesis, theanine biosynthesis is derived from nitrogen metabolism in Camellia sinensis
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ATP + L-glutamate + ethylamine
ADP + phosphate + N5-ethyl-L-glutamine
show the reaction diagram
-
-
-
-
?
ATP + L-glutamate + ethylamine
ADP + phosphate + N5-ethyl-L-glutamine
show the reaction diagram
DD410895
-
-
-
?
ATP + L-glutamate + ethylamine
ADP + phosphate + N5-ethyl-L-glutamine
show the reaction diagram
-
-
i.e. theanine
-
ATP + L-glutamate + ethylamine
ADP + phosphate + N5-ethyl-L-glutamine
show the reaction diagram
-
-
i.e. theanine
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ATP + L-glutamate + ethylamine
ADP + phosphate + N5-ethyl-L-glutamine
show the reaction diagram
-
-
-
-
?
ATP + L-glutamate + ethylamine
ADP + phosphate + N5-ethyl-L-glutamine
show the reaction diagram
DD410895
-
-
-
?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Mg2+
-
required
Mn2+
-
less effective than Mg2+ in activation
NaCl
-
theanine biosynthesis, derived from nitrogen metabolism in Camellia sinensis plants, can be influenced by salt treatment
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
beta-Alanine
-
weak
beta-phenylethylamine
-
-
Diaminobutane
-
weak
Diaminoethane
-
weak
ethanolamine
-
weak
Isobutylamine
-
-
Isopropylamine
-
-
L-alpha-alanine
-
weak
n-butylamine
-
-
n-Propylamine
-
-
NH4+
-
0.5 mM, complete inhibition
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.67
ATP
-
-
0.31
ethylamine
-
-
4.6
L-Glu
-
-
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
6.5 - 8.5
-
6.5: about 25% of maximal activity, 8.5: about 45% of maximal activity
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
-
main and lateral root, highest enzymic activity among all organs tested
Manually annotated by BRENDA team
-
substrates ethylamine and glutamic acid are distributed almost uniformly in all parts of the seedling
Manually annotated by BRENDA team
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
NaCl induces the enzyme expression in planta, levels of theanine and the total free amino acids are gradually increased in shoots, and reach the maximum on the 8th day after treatment
-
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
additional information
DD410895
long-term shade treatment induces the enzyme expression, whhich may be due to increased nitrogen assimilation and reduced theanine catabolism under shade conditions. The expression remains at a high level, overview