Information on EC 6.3.1.6 - Glutamate-ethylamine ligase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Mark a special word or phrase in this record:
Select one or more organisms in this record:
Show additional data
Do not include text mining results
Include (text mining) results (more...)
Include results (AMENDA + additional results, but less precise; more...)


The expected taxonomic range for this enzyme is: Camellia sinensis

EC NUMBER
COMMENTARY
6.3.1.6
-
RECOMMENDED NAME
GeneOntology No.
Glutamate-ethylamine ligase
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
ATP + L-glutamate + ethylamine = ADP + phosphate + N5-ethyl-L-glutamine
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
Acid amide hydrolysis
-
-
carboxylic
-
SYSTEMATIC NAME
IUBMB Comments
L-Glutamate:ethylamine ligase (ADP-forming)
-
SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
N5-Ethyl-L-glutamine synthetase
-
-
-
-
Synthetase, N5-ethylglutamine
-
-
-
-
Theanine synthetase
-
-
-
-
CAS REGISTRY NUMBER
COMMENTARY
62213-31-4
-
ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ATP + L-glutamate + ethylamine
ADP + phosphate + N5-ethyl-L-glutamine
show the reaction diagram
-
-
i.e. theanine
-
ATP + L-glutamate + ethylamine
ADP + phosphate + N5-ethyl-L-glutamine
show the reaction diagram
-
-
i.e. theanine
-
METALS and IONS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
Mg2+
-
required
Mn2+
-
less effective than Mg2+ in activation
INHIBITORS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
beta-Alanine
-
weak
beta-phenylethylamine
-
-
Diaminobutane
-
weak
Diaminoethane
-
weak
ethanolamine
-
weak
Isobutylamine
-
-
Isopropylamine
-
-
L-alpha-alanine
-
weak
n-butylamine
-
-
n-Propylamine
-
-
NH4+
-
0.5 mM, complete inhibition
KM VALUE [mM]
KM VALUE [mM] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.67
-
ATP
-
-
0.31
-
ethylamine
-
-
4.6
-
L-Glu
-
-
pH OPTIMUM
pH MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
pH RANGE
pH RANGE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
6.5
8.5
-
6.5: about 25% of maximal activity, 8.5: about 45% of maximal activity
SOURCE TISSUE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
SOURCE
-
main and lateral root, highest enzymic activity among all organs tested
Manually annotated by BRENDA team
-
substrates ethylamine and glutamic acid are distributed almost uniformly in all parts of the seedling
Manually annotated by BRENDA team