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Information on EC 5.4.99.30 - UDP-arabinopyranose mutase
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5.4.99.30 UDP-arabinopyranose mutaseIUBMB Comments
The reaction is reversible and at thermodynamic equilibrium the pyranose form is favored over the furanose form (90:10) .
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Word Map
- 5.4.99.30
- mutases
- polysaccharides
-
araf
- tabacum
- sativa
-
interconverts
-
plasmodesmata
- udp-sugars
-
furanose
- callose
- udp-galactose
- udp-glc
- arabinan
- xylan
- glucuronoarabinoxylans
- rhamnogalacturonan
-
monocot
-
pectic
- p-coumarates
-
ferulic
- l-arabinose
-
dicot
- arabinoxylans
-
stunted
-
photoassimilate
The expected taxonomic range for this enzyme is: Eukaryota, Archaea
Synonyms
udp-arabinopyranose mutase, osuam3, cruam, atrgp2, atrgp1, osuam1, bdrgp1, 
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
Bradi5g24850
LOC100822188
OsUAM1
OsUAM3
RGP1
RGP2
RGP3
RGP4
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
UDP-beta-L-arabinofuranose = UDP-beta-L-arabinopyranose
in functional UDP-arabinopyranose mutases a specific arginyl residue is reversibly glycosylated. This arginyl residue together with a DXD motif is required for catalytic activity
UDP-beta-L-arabinofuranose = UDP-beta-L-arabinopyranose
the reaction is reversible and at thermodynamic equilibrium the pyranose form is favored over the furanose form (90:10)
UDP-beta-L-arabinofuranose = UDP-beta-L-arabinopyranose
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-
-
-
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PATHWAY SOURCE
PATHWAYS
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SYSTEMATIC NAME
IUBMB Comments
UDP-arabinopyranose pyranomutase
The reaction is reversible and at thermodynamic equilibrium the pyranose form is favored over the furanose form (90:10) [1].
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
UDP-beta-L-arabinofuranose
UDP-beta-L-arabinopyranose
-
-
-
?
UDP-beta-L-arabinofuranose
UDP-beta-L-arabinopyranose
-
-
-
?
UDP-beta-L-arabinofuranose
UDP-beta-L-arabinopyranose
-
-
-
?
UDP-beta-L-arabinofuranose
UDP-beta-L-arabinopyranose
-
-
-
?
UDP-beta-L-arabinofuranose
UDP-beta-L-arabinopyranose
in functional UDP-arabinopyranose mutases a specific arginyl residue is reversibly glycosylated. This arginyl residue together with a DXD motif is required for catalytic activity
-
-
r
UDP-beta-L-arabinofuranose
UDP-beta-L-arabinopyranose
the ratio of UDP-beta-L-arabinopyranose to UDP-beta-L-arabinofuranose reaches a plateau at 93:7 from both pyranose- and furanose-forming directions
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-
r
UDP-beta-L-arabinofuranose
UDP-beta-L-arabinopyranose
the reaction is reversible and at thermodynamic equilibrium the pyranose form is favored over the furanose form (90:10)
-
-
r
UDP-beta-L-arabinofuranose
UDP-beta-L-arabinopyranose
the reaction is reversible and at thermodynamic equilibrium the pyranose form is favored over the furanose form (90:10)
-
-
?
UDP-beta-L-arabinofuranose
UDP-beta-L-arabinopyranose
-
-
-
r
UDP-beta-L-arabinofuranose
UDP-beta-L-arabinopyranose
-
-
-
?
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NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
UDP-beta-L-arabinofuranose
UDP-beta-L-arabinopyranose
-
-
-
?
UDP-beta-L-arabinofuranose
UDP-beta-L-arabinopyranose
-
-
-
?
UDP-beta-L-arabinofuranose
UDP-beta-L-arabinopyranose
-
-
-
?
UDP-beta-L-arabinofuranose
UDP-beta-L-arabinopyranose
-
-
-
r
UDP-beta-L-arabinofuranose
UDP-beta-L-arabinopyranose
-
-
-
?
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Ca2+
Co2+
Cu2+
Mg2+
Mn2+
Zn2+
additional information
Ca2+
displays less than 10% of the activity with Mn2+ at 0.08 mM, the activity does not increase at higher concentrations of Ca2+
Ca2+
displays less than 5% of the activity with Mn2+ at 0.08 mM, the activity does not increase at higher concentrations of Ca2+
Ca2+
displays less than 10% of the activity with Mn2+ at 0.08 mM, the activity does not increase at higher concentrations of Ca2+
Co2+
displays about 60% of the activity with Mn2+ at 0.08 mM
Co2+
displays about 65% of the activity with Mn2+ at 0.08 mM
Co2+
displays about 60% of the activity with Mn2+ at 0.08 mM
Cu2+
displays less than 10% of the activity with Mn2+ at 0.08 mM, the activity does not increase at higher concentrations of Cu2+
Cu2+
displays less than 5% of the activity with Mn2+ at 0.08 mM, the activity does not increase at higher concentrations of Cu2+
Cu2+
displays less than 10% of the activity with Mn2+ at 0.08 mM, the activity does not increase at higher concentrations of Cu2+
Mg2+
displays less than 10% of the activity with Mn2+ at 0.08 mM, the activity does not increase at higher concentrations of Mg2+
Mg2+
displays less than 5% of the activity with Mn2+ at 0.08 mM, the activity does not increase at higher concentrations of Mg2+
Mg2+
displays less than 10% of the activity with Mn2+ at 0.08 mM, the activity does not increase at higher concentrations of Mg2+
Mn2+
activates, best divalent metal ion, optimal at 0.08 mM, about 20% of maximal activity at 1 mM, complete inhibition at 5 mM
Mn2+
activates, best divalent metal ion, optimal at 0.08 mM, about 25% of maximal activity at 1 mM, complete inhibition at 5 mM
Mn2+
activates, best divalent metal ion, optimal at 0.08 mM, 45% of maximal activity at 5 mM
Zn2+
displays about 60% of the activity with Mn2+ at 0.08 mM
Zn2+
displays about 70% of the activity with Mn2+ at 0.08 mM
Zn2+
displays about 70% of the activity with Mn2+ at 0.08 mM
additional information
the enzyme requires divalent metal ions for catalytic activity, divalent metal ion dependency of UAM from Arabidopsis thaliana, compared to Oryza sativa
additional information
the enzyme requires divalent metal ions for catalytic activity, divalent metal ion dependency of UAM from Arabidopsis thaliana, compared to Oryza sativa
additional information
the enzyme requires divalent metal ions for catalytic activity, divalent metal ion dependency of UAM from Arabidopsis thaliana, compared to Oryza sativa
additional information
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the enzyme requires divalent metal ions for catalytic activity, divalent metal ion dependency of UAM from Arabidopsis thaliana, compared to Oryza sativa
additional information
no affect: CaCl2, MgCl2, CuSO4, ZnCl2, and CoCl2 at 5 mM
additional information
no affect: CaCl2, MgCl2, CuSO4, ZnCl2, and CoCl2 at 5 mM
additional information
-
no affect: CaCl2, MgCl2, CuSO4, ZnCl2, and CoCl2 at 5 mM
additional information
the enzyme requires divalent metal ions for catalytic activity, divalent metal ion dependency of UAM from Oryza sativa, compared to Arabidopsis thaliana
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INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
Mn2+
activates, best divalent metal ion, optimal at 0.08 mM, about 20% of maximal activity at 1 mM, complete inhibition at 5 mM; activates, best divalent metal ion, optimal at 0.08 mM, about 20% of maximal activity at 1 mM, complete inhibition at 5 mM; activates, best divalent metal ion, optimal at 0.08 mM, about 25% of maximal activity at 1 mM, complete inhibition at 5 mM
UDP
competitive inhibition, lower affinity for enzyme than substrate
UDP-Gal
decrease in mutase activity, when the nucleotide sugar reacts with the enzyme prior to the addition of beta-L-arabinofuranose; decrease in mutase activity, when the nucleotide sugar reacts with the enzyme prior to the addition of beta-L-arabinofuranose
UDP-Glc
decrease in mutase activity, when the nucleotide sugar reacts with the enzyme prior to the addition of beta-L-arabinofuranose; decrease in mutase activity, when the nucleotide sugar reacts with the enzyme prior to the addition of beta-L-arabinofuranose
UDP-Xyl
decrease in mutase activity, when the nucleotide sugar reacts with the enzyme prior to the addition of beta-L-arabinofuranose; decrease in mutase activity, when the nucleotide sugar reacts with the enzyme prior to the addition of beta-L-arabinofuranose
additional information
no affect: CaCl2, MgCl2, CuSO4, ZnCl2, and CoCl2 at 5 mM; no affect: CaCl2, MgCl2, CuSO4, ZnCl2, and CoCl2 at 5 mM
-
additional information
no affect: CaCl2, MgCl2, CuSO4, ZnCl2, and CoCl2 at 5 mM; no affect: CaCl2, MgCl2, CuSO4, ZnCl2, and CoCl2 at 5 mM
-
additional information
-
no affect: CaCl2, MgCl2, CuSO4, ZnCl2, and CoCl2 at 5 mM; no affect: CaCl2, MgCl2, CuSO4, ZnCl2, and CoCl2 at 5 mM
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
additional information
the combination of rUAM1, rUAM2, and rUAM3 increases activity 1.7fold as compared with that of the sum total of rUAM1, rUAM2, and rUAM3. This suggests that UAM isoenzymes work synergistically to increase activity
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additional information
the combination of rUAM1, rUAM2, and rUAM3 increases activity 1.7fold as compared with that of the sum total of rUAM1, rUAM2, and rUAM3. This suggests that UAM isoenzymes work synergistically to increase activity
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additional information
-
the combination of rUAM1, rUAM2, and rUAM3 increases activity 1.7fold as compared with that of the sum total of rUAM1, rUAM2, and rUAM3. This suggests that UAM isoenzymes work synergistically to increase activity
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DISEASE
TITLE OF PUBLICATION
LINK TO PUBMED
Anemia, Hypochromic
The constitutive expression of Arabidopsis plasmodesmal-associated class 1 reversibly glycosylated polypeptide impairs plant development and virus spread.
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
additional information
additional information
RGP1 shows higher enzymatic activity than isoenzyme RGP2. RGP3 shows a very low enzymatic activity
additional information
RGP1 shows higher enzymatic activity than isoenzyme RGP2. RGP3 shows a very low enzymatic activity
additional information
RGP1 shows higher enzymatic activity than isoenzyme RGP2. RGP3 shows a very low enzymatic activity
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
6.8
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pH RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
4 - 8
pH 4.0: about 40% of maximal activity, pH 8.0: about 55% of maximal activity, pyranose forming activity
5 - 8
pH 5.0: about 50% of maximal activity, pH 8.0: about 50% of maximal activity, furanose-forming activity
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TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
55
60
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TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
20 - 100
over 90% of maximal activity at 40-90°C, 60% at 20°C and 100°C, profile overview
20 - 90
over 50% of maximal activity within this range, profile overview
30 - 60
30°C: about 50% of maximal activity, 60°C: about 85% of maximal activity
30 - 95
over 80% of maximal activity within this range, 40% at 15°C, over 90% at 35-85°C, profile overview
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ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
OsUAM1 and OsUAM2 are expressed ubiquitously throughout plant development, but OsUAM3 is expressed primarily in reproductive tissue, particularly at the pollen cell wall formation developmental stage
brenda
additional information
almost exclusively detected in siliques, with the highest levels 6 to 8 d post anthesis
brenda
additional information
some of the AtRGPs such as the more temperature stable AtRGP2 and tRGP3 may be expressed by the plant as a response to heat stress, or during different stages of development
brenda
additional information
some of the AtRGPs such as the more temperature stable AtRGP2 and tRGP3 may be expressed by the plant as a response to heat stress, or during different stages of development
brenda
additional information
some of the AtRGPs such as the more temperature stable AtRGP2 and tRGP3 may be expressed by the plant as a response to heat stress, or during different stages of development
brenda
additional information
-
some of the AtRGPs such as the more temperature stable AtRGP2 and tRGP3 may be expressed by the plant as a response to heat stress, or during different stages of development
brenda
additional information
isozyme RGP3 expression is present in sheath and stem samples but not leaf blade samples regardless of developmental stage, RGP isozymes expression patterns in plant tissues, quantitative real-time PCR enzyme expression analysis, overview. RGP3 shows a low expression compared to the other isozymes
brenda
additional information
isozyme RGP3 expression is present in sheath and stem samples but not leaf blade samples regardless of developmental stage, RGP isozymes expression patterns in plant tissues, quantitative real-time PCR enzyme expression analysis, overview. RGP3 shows a low expression compared to the other isozymes
brenda
additional information
isozyme RGP3 expression is present in sheath and stem samples but not leaf blade samples regardless of developmental stage, RGP isozymes expression patterns in plant tissues, quantitative real-time PCR enzyme expression analysis, overview. RGP3 shows a low expression compared to the other isozymes
brenda
additional information
isozyme RGP3 expression is present in sheath and stem samples but not leaf blade samples regardless of developmental stage, RGP isozymes expression patterns in plant tissues, quantitative real-time PCR enzyme expression analysis, overview. RGP3 shows a low expression compared to the other isozymes
brenda
additional information
-
isozyme RGP3 expression is present in sheath and stem samples but not leaf blade samples regardless of developmental stage, RGP isozymes expression patterns in plant tissues, quantitative real-time PCR enzyme expression analysis, overview. RGP3 shows a low expression compared to the other isozymes
brenda
additional information
RGP isozymes expression patterns in plant tissues, quantitative real-time PCR enzyme expression analysis, overview
brenda
additional information
RGP isozymes expression patterns in plant tissues, quantitative real-time PCR enzyme expression analysis, overview
brenda
additional information
RGP isozymes expression patterns in plant tissues, quantitative real-time PCR enzyme expression analysis, overview
brenda
additional information
RGP isozymes expression patterns in plant tissues, quantitative real-time PCR enzyme expression analysis, overview
brenda
additional information
-
RGP isozymes expression patterns in plant tissues, quantitative real-time PCR enzyme expression analysis, overview
brenda
additional information
-
RGP isozymes expression patterns in plant tissues, quantitative real-time PCR enzyme expression analysis, overview
-
brenda
additional information
OsUAM3 coexpression networks include pectin catabolic enzymes. Co-expression network in silico analysis, quantitative RT-PCR analysis of OsUAM isozymes gene expression in various organs at different developmental stages, overview
brenda
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LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
plant cell wall and leaf cell wall
brenda
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GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
evolution
malfunction
physiological function
additional information
three-dimensional structure modeling of isozyme UAM1
evolution
the UAM gene family has three members in Oryza sativa
malfunction
Arabidopsis lines with reduced RGP1 and/or RGP2 expression are deficient in cell wall L-arabinose and exhibit severe developmental defects
malfunction
-
RNAi is used to downregulate OsUAM gene expression. This leads to a reduction of between 6 and 44% in the amounts of arabinofuranose in the cell wall, a decrease in the extent of substitution of the xylan backbone, and a reduction of between 25% and 80% in the ferulic acid or p-coumaric acid contents of the wall. Transgenic rice plants with a >25% reduction in arabinose content are dwarfed and infertile
malfunction
OsUAM3-knockdown plants grow normally, but show abnormal phenotypes in reproductive tissues, especially in terms of the pollen cell wall and exine. Pollen formation is abnormal in OsUAM3-KD plants, and KI-I2 staining for starch content shows that OsUAM3-KD pollen is abnormal and accumulates little starch. Low levels of arabinan in OsUAM3-KD pollen grains
malfunction
RNAi mutants of isozyme BdRGP1 result in large alterations in cell wall carbohydrate composition with significant decreases in cell wall arabinose content but with minimal change in plant stature. Real-time PCR analysis indicates that gene expression levels for BdRGP1, BdRGP2, and BdRGP3 are reduced in RNAi-RGP1 plants to 15-20%of controls. Cell wall arabinose content is reduced by 23-51%of control levels. No alterations in cell wall xylose and glucose content are observed. Corresponding decreases in cell wall ferulic acid and ferulic acid-dimers are observed. Additionally, cell wall 4-coumarates are decreased. The cell wall decrease in 4-coumarates corresponds to arabinose-coupled 4-coumarates. Xylanase-mediated digestibility of RNAi-RGP1 Brachypodium cell walls results in a near 2fold increase of released total carbohydrate. Cellulolytic hydrolysis of cell wall material is inhibited in leaves of RNAi-RGP1 mutants
malfunction
-
the NtUAMKD mutant, with all four isozymes knocked out, shows abnormal leaf development in the form of a callus-like structure and many holes in the leaf epidermis. A clear reduction in the pectic arabinan content is observed in the tissue of the NtUAM-KD leaf. The arabinose/xylose ratio in the xyloglucan-rich cell wall fraction is drastically reduced in NtUAM-KD
physiological function
isozyme OsUAM3 has a different function from that of isozymes OsUAM1 and OsUAM2i, sozyme UDP-arabinopyranose mutase 3 is required for pollen wall morphogenesis in Oryza sativa. L-Arabinose is one of the main constituents of cell wall polysaccharides such as pectic rhamnogalacturonan I, RG-I, glucuronoarabinoxylans and other glycoproteins. It is found predominantly in the furanose form rather than in the thermodynamically more stable pyranose form. UDP-L-arabinofuranose (UDP-Araf), rather than UDP-L-arabinopyranose (UDP-Arap), is a sugar donor for the biosynthesis of arabinofuranosyl (Araf) residues. UDP-arabinopyranose mutases (UAMs) interconvert UDP-Araf and UDP-Arap and are involved in the biosynthesis of polysaccharides including Araf
physiological function
the enzyme is involved in cell wall carbohydrate biosynthesis, the cell wall composition has a complex effect on digestibility of grass plants
physiological function
-
UDP-arabinopyranose mutase gene expressions are required for the biosynthesis of the arabinose side chain of both pectin and arabinoxyloglucan, and normal leaf expansion in Nicotiana tabacum
Show AA Sequence and Transmembrane Helices (1161 entries)
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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
460000
mutase is likely to exist as a complex composed of numerous proteins, gel filtration
additional information
additional information
native mutase is likely to exist as a complex composed of several different UDP-arabinopyranose mutases
additional information
native mutase is likely to exist as a complex composed of several different UDP-arabinopyranose mutases
additional information
-
native mutase is likely to exist as a complex composed of several different UDP-arabinopyranose mutases
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SUBUNIT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
glycoprotein
glycoprotein
the enzyme is a reversibly glycosylated protein
glycoprotein
autoglycosylation occurs at a specific arginyl residue in recombinant UDP-arabinopyranose mutase that is essential for mutase activity
glycoprotein
rUAM1 is autoglycosylated when each protein reacts separately with UDP-Glc. Reversibly glycosylated in the presence of UDP-Glc
glycoprotein
rUAM3 is autoglycosylated when each protein reacts separately with UDP-Glc. Reversibly glycosylated in the presence of UDP-Glc
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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
additional information
additional information
specific knockout of the isozyme by RNAi-mediated gene-suppression, overexpression by constitutive expression approach, phenotypes, phenotypes, detailed overview. Analysis of phenotypes for generation of UDP-sugar interconversion pathway transgenic Brachypodium distachyon lines resulting in cell wall carbohydrate composition changes with improved digestibility and normal plant stature
additional information
specific knockout of the isozyme by RNAi-mediated gene-suppression, overexpression by constitutive expression approach, phenotypes, phenotypes, detailed overview. Analysis of phenotypes for generation of UDP-sugar interconversion pathway transgenic Brachypodium distachyon lines resulting in cell wall carbohydrate composition changes with improved digestibility and normal plant stature
additional information
specific knockout of the isozyme by RNAi-mediated gene-suppression, overexpression by constitutive expression approach, phenotypes, phenotypes, detailed overview. Analysis of phenotypes for generation of UDP-sugar interconversion pathway transgenic Brachypodium distachyon lines resulting in cell wall carbohydrate composition changes with improved digestibility and normal plant stature
additional information
specific knockout of the isozyme by RNAi-mediated gene-suppression, overexpression by constitutive expression approach, phenotypes, phenotypes, detailed overview. Analysis of phenotypes for generation of UDP-sugar interconversion pathway transgenic Brachypodium distachyon lines resulting in cell wall carbohydrate composition changes with improved digestibility and normal plant stature
additional information
-
specific knockout of the isozyme by RNAi-mediated gene-suppression, overexpression by constitutive expression approach, phenotypes, phenotypes, detailed overview. Analysis of phenotypes for generation of UDP-sugar interconversion pathway transgenic Brachypodium distachyon lines resulting in cell wall carbohydrate composition changes with improved digestibility and normal plant stature
additional information
specific knockout of the isozyme by RNAi-mediated gene-suppression, overexpression by constitutive expression approach, phenotypes, phenotypes, detailed overview. Analysis of phenotypes for generation of UDP-sugar interconversion pathway transgenic Brachypodium distachyon lines resulting in cell wall carbohydrate composition changes with improved digestibility and normal plant stature. Targeted manipulation of UDP-sugar biosynthesis can result in biomass with substantially altered compositions
additional information
specific knockout of the isozyme by RNAi-mediated gene-suppression, overexpression by constitutive expression approach, phenotypes, phenotypes, detailed overview. Analysis of phenotypes for generation of UDP-sugar interconversion pathway transgenic Brachypodium distachyon lines resulting in cell wall carbohydrate composition changes with improved digestibility and normal plant stature. Targeted manipulation of UDP-sugar biosynthesis can result in biomass with substantially altered compositions
additional information
specific knockout of the isozyme by RNAi-mediated gene-suppression, overexpression by constitutive expression approach, phenotypes, phenotypes, detailed overview. Analysis of phenotypes for generation of UDP-sugar interconversion pathway transgenic Brachypodium distachyon lines resulting in cell wall carbohydrate composition changes with improved digestibility and normal plant stature. Targeted manipulation of UDP-sugar biosynthesis can result in biomass with substantially altered compositions
additional information
specific knockout of the isozyme by RNAi-mediated gene-suppression, overexpression by constitutive expression approach, phenotypes, phenotypes, detailed overview. Analysis of phenotypes for generation of UDP-sugar interconversion pathway transgenic Brachypodium distachyon lines resulting in cell wall carbohydrate composition changes with improved digestibility and normal plant stature. Targeted manipulation of UDP-sugar biosynthesis can result in biomass with substantially altered compositions
additional information
-
specific knockout of the isozyme by RNAi-mediated gene-suppression, overexpression by constitutive expression approach, phenotypes, phenotypes, detailed overview. Analysis of phenotypes for generation of UDP-sugar interconversion pathway transgenic Brachypodium distachyon lines resulting in cell wall carbohydrate composition changes with improved digestibility and normal plant stature. Targeted manipulation of UDP-sugar biosynthesis can result in biomass with substantially altered compositions
additional information
-
specific knockout of the isozyme by RNAi-mediated gene-suppression, overexpression by constitutive expression approach, phenotypes, phenotypes, detailed overview. Analysis of phenotypes for generation of UDP-sugar interconversion pathway transgenic Brachypodium distachyon lines resulting in cell wall carbohydrate composition changes with improved digestibility and normal plant stature
-
additional information
-
generation of RNAi transformants (NtUAM-KD) to downregulate all four of the UAM members, phenotype, genetic RNAi constructs, overview. Downregulation of NtUAMs expression effects on the localization of arabinan in leaf, and NtUAM knockdown causes the abnormal callus and hole in epidermal tissues
additional information
RNA interference (RNAi)-knockdown transformants (OsUAM3-KD) specific for OsUAM3. Modifications of cell wall polysaccharides at the cellular level are determined using antibodies against polysaccharides including Araf, low levels of arabinan in OsUAM3-KD pollen grains
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TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
repeated cycles of freezing and thawing did not significantly affect mutase activity
rUMA1 expressed in insect cells retains activity after repeated cycles of freezing and thawing
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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
purified 94fold using hydrophobic chromatography and gel filtration
-
recombinant His-MBP-tagged isozyme RGP1 from Escherichia coli strain BL21 Gold by nickel affinity chromatography, tag cleavage with TEV protease, followed by another step of nickel affinity chromatography, and gel filtration
recombinant His-MBP-tagged isozyme RGP2 from Escherichia coli strain BL21 Gold by nickel affinity chromatography, tag cleavage with TEV protease, followed by another step of nickel affinity chromatography, and gel filtration
recombinant His-MBP-tagged isozyme RGP3 from Escherichia coli strain BL21 Gold by nickel affinity chromatography, tag cleavage with TEV protease, followed by another step of nickel affinity chromatography, and gel filtration
recombinant His-tagged wild-type and mutant isozymes UAM1 from Escherichia coli strain BL21 by nickel affinity chromatography
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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli
expressed in Escherichia coli as a His-tagged fusion protein
gene RGP1, recombinant overexpression of His-MBP-tagged isozyme in Escherichia coli strain BL21 Gold
gene RGP2, recombinant overexpression of His-MBP-tagged isozyme in Escherichia coli strain BL21 Gold
gene RGP3, recombinant overexpression of His-MBP-tagged isozyme in Escherichia coli strain BL21 Gold
gene UAM3 or RGP3, OsUAM3 expression is independent from OsUAM1 and OsUAM2 co-expression networks
genes UAM1-4, sequence comparisons and phylogenetic tree, subcloning in Escherichia coli strain DH5alpha, recombinant expression in of RNAi constructs for all 4 isozymes UAM1-4 in Nicotiana tabacum via Agrobacterium tumefaciens strain GV2260-mediated transformation, RT-PCR expression analysis
-
isozyme RGP1, recombinant overexpression in Brachypodium distachyon strain Bd21-3 using the Agrobacterum tumefaciens strain AGL1 transformation method, quantitative real-time PCR enzyme expression analysis
isozyme RGP2, recombinant overexpression in Brachypodium distachyon strain Bd21-3 using the Agrobacterum tumefaciens strain AGL1 method, quantitative real-time PCR enzyme expression analysis
isozyme RGP3, recombinant overexpression in Brachypodium distachyon strain Bd21-3 using the Agrobacterum tumefaciens strain AGL1 transformation method, quantitative real-time PCR enzyme expression analysis
isozyme RGP4, recombinant overexpression in Brachypodium distachyon strain Bd21-3 using the Agrobacterum tumefaciens strain AGL1 transformation method, quantitative real-time PCR enzyme expression analysis
isozyme UAM1, recombinant expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21
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EXPRESSION
ORGANISM
UNIPROT
LITERATURE
some of the AtRGPs such as the more temperature stable AtRGP2 and tRGP3 may be expressed by the plant as a response to heat stress, or during different stages of development
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REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Konishi, T.; Miyazaki, Y.; Yamakawa, S.; Iwai, H.; Satoh, S.; Ishii, T.
Purification and biochemical characterization of recombinant rice UDP-arabinopyranose mutase generated in insect cells
Biosci. Biotechnol. Biochem.
74
191-194
2010
Oryza sativa (Q6Z4G3), Oryza sativa (Q8H8T0), Oryza sativa
brenda
Konishi, T.; Ohnishi-Kameyama, M.; Funane, K.; Miyazaki, Y.; Konishi, T.; Ishii, T.
An arginyl residue in rice UDP-arabinopyranose mutase is required for catalytic activity and autoglycosylation
Carbohydr. Res.
345
787-791
2010
Oryza sativa (Q6Z4G3), Oryza sativa (Q8H8T0), Oryza sativa
brenda
Konishi, T.; Takeda, T.; Miyazaki, Y.; Ohnishi-Kameyama, M.; Hayashi, T.; O'Neill, M.A.; Ishii, T.
A plant mutase that interconverts UDP-arabinofuranose and UDP-arabinopyranose
Glycobiology
17
345-354
2006
Oryza sativa (O82706), Oryza sativa (Q8H8T0), Oryza sativa
brenda
Kotani, A.; Tsuji, M.; Azama, Y.; Ishii, T.; Takeda, T.; Yamashita, T.; Shimojima, M.; Konishi, T.
Purification and characterization of UDP-arabinopyranose mutase from Chlamydomonas reinhardtii
Biosci. Biotechnol. Biochem.
77
1874-1878
2013
Chlamydomonas reinhardtii
brenda
Konishi, T.; Aohara, T.; Igasaki, T.; Hayashi, N.; Miyazaki, Y.; Takahashi, A.; Hirochika, H.; Iwai, H.; Satoh, S.; Ishii, T.
Down-regulation of UDP-arabinopyranose mutase reduces the proportion of arabinofuranose present in rice cell walls
Phytochemistry
72
1962-1968
2011
Oryza sativa
brenda
Rautengarten, C.; Ebert, B.; Herter, T.; Petzold, C.J.; Ishii, T.; Mukhopadhyay, A.; Usadel, B.; Scheller, H.V.
The interconversion of UDP-arabinopyranose and UDP-arabinofuranose is indispensable for plant development in Arabidopsis
Plant Cell
23
1373-1390
2011
Arabidopsis thaliana (O22666), Arabidopsis thaliana (Q9LFW1), Arabidopsis thaliana (Q9SRT9)
brenda
Kuttiyatveetil, J.R.A.; Sanders, D.A.R.
Analysis of plant UDP-arabinopyranose mutase (UAM) Role of divalent metals and structure prediction
Biochim. Biophys. Acta
1865
510-519
2017
Arabidopsis thaliana (O22666), Arabidopsis thaliana (Q9LFW1), Arabidopsis thaliana (Q9SRT9), Arabidopsis thaliana, Oryza sativa Japonica Group (Q8H8T0)
brenda
Rancour, D.M.; Hatfield, R.D.; Marita, J.M.; Rohr, N.A.; Schmitz, R.J.
Cell wall composition and digestibility alterations in Brachypodium distachyon achieved through reduced expression of the UDP-arabinopyranose mutase
Front. Plant Sci.
6
446
2015
Brachypodium distachyon (I1GQE9), Brachypodium distachyon (I1GSJ4), Brachypodium distachyon (I1HRV4), Brachypodium distachyon (I1J2W9), Brachypodium distachyon, Brachypodium distachyon Bd21 (I1J2W9)
brenda
Honta, H.; Inamura, T.; Konishi, T.; Satoh, S.; Iwai, H.
UDP-arabinopyranose mutase gene expressions are required for the biosynthesis of the arabinose side chain of both pectin and arabinoxyloglucan, and normal leaf expansion in Nicotiana tabacum
J. Plant Res.
131
307-317
2018
Nicotiana tabacum
brenda
Sumiyoshi, M.; Inamura, T.; Nakamura, A.; Aohara, T.; Ishii, T.; Satoh, S.; Iwai, H.
UDP-arabinopyranose mutase 3 is required for pollen wall morphogenesis in rice (Oryza sativa)
Plant Cell Physiol.
56
232-241
2015
Oryza sativa Japonica Group (Q6Z4G3)
brenda
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