Information on EC 5.4.4.2 - Isochorismate synthase

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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria

EC NUMBER
COMMENTARY
5.4.4.2
-
RECOMMENDED NAME
GeneOntology No.
Isochorismate synthase
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
Chorismate = isochorismate
show the reaction diagram
-
-
-
-
Chorismate = isochorismate
show the reaction diagram
K90 is the base that activates water for nucleophilic attack at the chorismate C2 carbon
P38051
REACTION TYPE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
group transfer
-
-
intramolecular
-
isomerization
-
-
-
-
PATHWAY
KEGG Link
MetaCyc Link
1,4-dihydroxy-2-naphthoate biosynthesis I
-
1,4-dihydroxy-2-naphthoate biosynthesis II (plants)
-
2,3-dihydroxybenzoate biosynthesis
-
Biosynthesis of secondary metabolites
-
Biosynthesis of siderophore group nonribosomal peptides
-
Metabolic pathways
-
salicylate biosynthesis I
-
Ubiquinone and other terpenoid-quinone biosynthesis
-
SYSTEMATIC NAME
IUBMB Comments
Isochorismate hydroxymutase
Requires Mg2+. The reaction is reversible.
SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
Amonabactin
-
-
-
-
EC 5.4.99.6
-
-
formerly
-
Eds16
-
-
-
-
Enhanced disease susceptibility 16
-
-
-
-
EntC
P0AEJ2
-
ICS
P0AEJ2
-
ICS1
-
isoform
ICS1
Q9M9V6
isozyme
ICS1 gene product
Q9S7H8
-
ICS1 gene product
-
-
ICS2
-
isoform
ICS2
Q9M9V6
isozyme
IcsI
-
-
-
-
isochorismate mutase
-
-
-
-
isochorismate synthase
Q9S7H8
-
isochorismate synthase
-
-
isochorismate synthase 1
-
-
Isochorismic synthase
-
-
-
-
menaquinone-specific isochorismate synthase
P38051
-
MenF
P38051
-
Sid2
-
-
-
-
Synthase, isochorismate
-
-
-
-
CAS REGISTRY NUMBER
COMMENTARY
37318-53-9
-
ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
activity is not regulated by light-dependent changes in stromal pH value, Mg2+ or redox potential
SwissProt
Manually annotated by BRENDA team
ecotype Col-0
-
-
Manually annotated by BRENDA team
duplicate isochorismate genes, menF and dhbC. Wild-type strain and mutants carrying deletions of menF or dhbC
-
-
Manually annotated by BRENDA team
isochorismate synthase involved in enterobactin biosynthesis, MenF. The isochorismate synthase involved in menaquinone biosynthesis is different from the isochorismate synthase involved in enterobactin biosynthesis
-
-
Manually annotated by BRENDA team
isochorismate synthase involved in menaquinone biosynthesis, EntC
-
-
Manually annotated by BRENDA team
menaquinone-specific enzyme
SwissProt
Manually annotated by BRENDA team
strain AN191 and strain AN92
-
-
Manually annotated by BRENDA team
strain K-12
UniProt
Manually annotated by BRENDA team
K3-15; strain 238-7
-
-
Manually annotated by BRENDA team
Flavobacterium sp. 238-7
strain 238-7
-
-
Manually annotated by BRENDA team
Flavobacterium sp. K3-15
K3-15
-
-
Manually annotated by BRENDA team
enzyme is found in anthraquinone-producing cell line, but not in a non-producing cell line
-
-
Manually annotated by BRENDA team
kcat/KM VALUE [1/mMs-1]
kcat/KM VALUE [1/mMs-1] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.11
-
chorismate
P0AEJ2
mutant enzyme F327Y/I346L, in 100 mM phosphate pH 7.0, 10 mM MgCl2
8588
0.65
-
chorismate
P0AEJ2
mutant enzyme A303T, in 100 mM phosphate pH 7.0, 10 mM MgCl2
8588
0.69
-
chorismate
P0AEJ2
mutant enzyme F359Q/I346L, in 100 mM phosphate pH 7.0, 10 mM MgCl2
8588
1.71
-
chorismate
P0AEJ2
mutant enzyme F327Y, in 100 mM phosphate pH 7.0, 10 mM MgCl2
8588
7.24
-
chorismate
P0AEJ2
mutant enzyme I346L, in 100 mM phosphate pH 7.0, 10 mM MgCl2
8588
9.1
-
chorismate
P0AEJ2
mutant enzyme F359Q, in 100 mM phosphate pH 7.0, 10 mM MgCl2
8588
10.75
-
chorismate
P0AEJ2
mutant enzyme F327Y/F359Q, in 100 mM phosphate pH 7.0, 10 mM MgCl2
8588
95.7
-
chorismate
P0AEJ2
wild type enzyme, in 100 mM phosphate pH 7.0, 10 mM MgCl2
8588
Ki VALUE [mM]
Ki VALUE [mM] Maximum
INHIBITOR
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.03
-
(4R,5R)-4-hydroxy-5-(1-carboxyvinyloxy)-cyclohex-1-ene carboxylate
-
-
2
-
(4R,5R)-4-hydroxy-5-carboxymethoxy-cyclohex-1-enecarboxylate
-
IC50 above 2.0 mM
2
-
(4R,5R)-5-(2-carboxy-allyloxy)-4-hydroxy-cyclohex-1-enecarboxylate
-
IC50 above 2.0 mM
0.00005
-
(4R,5R,6S)-6-ammonio-5-[(1-carboxylatoethenyl)oxy]-4-hydroxycyclohex-1-ene-1-carboxylate
-
-
2
-
(4R,5R,7R)-5-(1-carboxy-ethoxy)-4-hydroxy-cyclohex-1-enecarboxylate
-
IC50 above 2.0 mM
2
-
(4R,5R,7S)-5-(1-carboxy-ethoxy)-4-hydroxy-cyclohex-1-enecarboxylate
-
IC50 above 2.0 mM
0.00045
-
(4R,5S,6S)-4-ammonio-5-[(1-carboxylatoethenyl)oxy]-6-hydroxycyclohex-1-ene-1-carboxylate
-
-
0.00036
-
(4R,5S,6S)-5-[(1-carboxylatoethenyl)oxy]-4,6-dihydroxycyclohex-1-ene-1-carboxylate
-
-
pH RANGE
pH RANGE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
5.5
8.5
-
pH 5.5: about 20% of maximal activity, pH 8.5: about 90% of maximal activity
5.5
8.8
-
about 50% of maximal activity at pH 5.5 and at pH 8.8
PDB
SCOP
CATH
ORGANISM
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
TEMPERATURE STABILITY
TEMPERATURE STABILITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
4
-
-
half-life of the enzyme immobilized on alkylamine glass: 210 days. Half-life of enzyme immobilized on CNBr Sepharose: 110 days
37
-
-
40 h, immobilized enzyme, stable
GENERAL STABILITY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
stability of the enzyme is greatly increased by immobilization
-
no activity in presence of 20 mM ascorbate or 5 mM thiourea. 43% decrease in activity in presence of 10 mM Cys
-
glycerol, EDTA and dithiothreitol stabilize
-
STORAGE STABILITY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
-20C, 40% loss of activity after 5 days. 1% bovine serum albumin, 10% glycerol, or 20% dimethyl sulfoxide stabilize for up to 10 days
-
4C or -20C, 50% loss of activity after 4 days
-
-80C, stable for at least 2 months
-