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1-(5-phospho-beta-D-ribosyl)-5-[(5-phospho-beta-D-ribosylamino)methylideneamino]imidazole-4-carboxamide = 5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide
1-(5-phospho-beta-D-ribosyl)-5-[(5-phospho-beta-D-ribosylamino)methylideneamino]imidazole-4-carboxamide = 5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide
-
-
-
-
1-(5-phospho-beta-D-ribosyl)-5-[(5-phospho-beta-D-ribosylamino)methylideneamino]imidazole-4-carboxamide = 5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide
Amadori rearrangement
-
1-(5-phospho-beta-D-ribosyl)-5-[(5-phospho-beta-D-ribosylamino)methylideneamino]imidazole-4-carboxamide = 5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide
enzyme catalyzes Amadori rearrangement of a thermolabile aminoaldolase into the corresponding aminoketose, reaction mechanism involving general acid-base catalysis and a Schiff base intermediate is proposed
-
1-(5-phospho-beta-D-ribosyl)-5-[(5-phospho-beta-D-ribosylamino)methylideneamino]imidazole-4-carboxamide = 5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide
enzyme catalyzes Amadori rearrangement of a thermolabile aminoaldolase into the corresponding aminoketose, reaction mechanism involving general acid-base catalysis and a Schiff base intermediate is proposed
-
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1-(5-phospho-beta-D-ribosyl)-5-[(5-phospho-beta-D-ribosylamino)methylideneamino]imidazole-4-carboxamide
5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide
1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino]imidazole-4-carboxamide
5-[(5-phospho-1-deoxyribulos-1-ylamino)methylideneamino]-1-(5-phosphoribosyl)imidazole-4-carboxamide
N'-[(5'-phosphoribosyl)formimino]-5-aminoimidazole-4-carboxamide ribonucleotide
?
N-(5'-Phospho-D-ribosylformimino)-5-amino-1-(5''-phospho-D-ribosyl)-4-imidazolecarboxamide
?
-
enzyme catalyzes the fourth step in histidine biosynthesis
-
-
?
N-(5'-Phospho-D-ribosylformimino)-5-amino-1-(5''-phospho-D-ribosyl)-4-imidazolecarboxamide
N-(5'-Phospho-D-1-ribulosylformimino)-5-amino-1-(5''-phospho-D-ribosyl)-4-imidazolecarboxamide
additional information
?
-
1-(5-phospho-beta-D-ribosyl)-5-[(5-phospho-beta-D-ribosylamino)methylideneamino]imidazole-4-carboxamide
5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide
-
-
-
-
?
1-(5-phospho-beta-D-ribosyl)-5-[(5-phospho-beta-D-ribosylamino)methylideneamino]imidazole-4-carboxamide
5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide
-
-
-
-
?
1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino]imidazole-4-carboxamide
5-[(5-phospho-1-deoxyribulos-1-ylamino)methylideneamino]-1-(5-phosphoribosyl)imidazole-4-carboxamide
the enzyme catalyzes the fourth step of the His biosynthetic pathway
-
?
1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino]imidazole-4-carboxamide
5-[(5-phospho-1-deoxyribulos-1-ylamino)methylideneamino]-1-(5-phosphoribosyl)imidazole-4-carboxamide
ProFAR, aminoaldose
PRFAR, aminoketose
-
r
N'-[(5'-phosphoribosyl)formimino]-5-aminoimidazole-4-carboxamide ribonucleotide
?
-
-
-
?
N'-[(5'-phosphoribosyl)formimino]-5-aminoimidazole-4-carboxamide ribonucleotide
?
-
-
-
?
N-(5'-Phospho-D-ribosylformimino)-5-amino-1-(5''-phospho-D-ribosyl)-4-imidazolecarboxamide
N-(5'-Phospho-D-1-ribulosylformimino)-5-amino-1-(5''-phospho-D-ribosyl)-4-imidazolecarboxamide
-
-
-
?
N-(5'-Phospho-D-ribosylformimino)-5-amino-1-(5''-phospho-D-ribosyl)-4-imidazolecarboxamide
N-(5'-Phospho-D-1-ribulosylformimino)-5-amino-1-(5''-phospho-D-ribosyl)-4-imidazolecarboxamide
-
-
-
?
additional information
?
-
APG10 knockout mutant exhibits embryo lethality, indicating the essential role of the Arabidopsis BBMII isomerase for plant growth
-
-
?
additional information
?
-
-
APG10 knockout mutant exhibits embryo lethality, indicating the essential role of the Arabidopsis BBMII isomerase for plant growth
-
-
?
additional information
?
-
-
enzyme catalyzes Amadori rearrangements of a thermolabile aminoaldolase into the corresponding aminoketose, reaction mechanism involving general acid-base catalysis and a Schiff base intermediate is poposed
-
?
additional information
?
-
-
the enzyme is involved in histidine biosynthesis
-
?
additional information
?
-
-
bifunctional enzyme with activity of EC 5.3.1.16 and EC 5.3.1.24
-
?
additional information
?
-
-
bifunctional enzyme with activity of EC 5.3.1.16 and EC 5.3.1.24 has a dual function in both histidine and aromatic amino acid biosynthesis
-
?
additional information
?
-
-
bifunctional enzyme with activity of EC 5.3.1.16 and EC 5.3.1.24
-
?
additional information
?
-
-
bifunctional enzyme with activity of EC 5.3.1.16 and EC 5.3.1.24 has a dual function in both histidine and aromatic amino acid biosynthesis
-
?
additional information
?
-
-
bifunctional enzyme with activity of EC 5.3.1.16 and EC 5.3.1.24
-
?
additional information
?
-
-
bifunctional enzyme with activity of EC 5.3.1.16 and EC 5.3.1.24 has a dual function in both histidine and aromatic amino acid biosynthesis
-
?
additional information
?
-
-
enzyme of histidine biosynthesis
-
?
additional information
?
-
-
the enzyme is involved in histidine biosynthesis
-
?
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1-(5-phospho-beta-D-ribosyl)-5-[(5-phospho-beta-D-ribosylamino)methylideneamino]imidazole-4-carboxamide
5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide
1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino]imidazole-4-carboxamide
5-[(5-phospho-1-deoxyribulos-1-ylamino)methylideneamino]-1-(5-phosphoribosyl)imidazole-4-carboxamide
the enzyme catalyzes the fourth step of the His biosynthetic pathway
-
?
N-(5'-Phospho-D-ribosylformimino)-5-amino-1-(5''-phospho-D-ribosyl)-4-imidazolecarboxamide
?
-
enzyme catalyzes the fourth step in histidine biosynthesis
-
-
?
additional information
?
-
1-(5-phospho-beta-D-ribosyl)-5-[(5-phospho-beta-D-ribosylamino)methylideneamino]imidazole-4-carboxamide
5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide
-
-
-
-
?
1-(5-phospho-beta-D-ribosyl)-5-[(5-phospho-beta-D-ribosylamino)methylideneamino]imidazole-4-carboxamide
5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide
-
-
-
-
?
additional information
?
-
APG10 knockout mutant exhibits embryo lethality, indicating the essential role of the Arabidopsis BBMII isomerase for plant growth
-
-
?
additional information
?
-
-
APG10 knockout mutant exhibits embryo lethality, indicating the essential role of the Arabidopsis BBMII isomerase for plant growth
-
-
?
additional information
?
-
-
the enzyme is involved in histidine biosynthesis
-
?
additional information
?
-
-
bifunctional enzyme with activity of EC 5.3.1.16 and EC 5.3.1.24 has a dual function in both histidine and aromatic amino acid biosynthesis
-
?
additional information
?
-
-
bifunctional enzyme with activity of EC 5.3.1.16 and EC 5.3.1.24 has a dual function in both histidine and aromatic amino acid biosynthesis
-
?
additional information
?
-
-
bifunctional enzyme with activity of EC 5.3.1.16 and EC 5.3.1.24 has a dual function in both histidine and aromatic amino acid biosynthesis
-
?
additional information
?
-
-
enzyme of histidine biosynthesis
-
?
additional information
?
-
-
the enzyme is involved in histidine biosynthesis
-
?
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0.6 - 37.9
N'-[(5'-phosphoribosyl)formimino]-5-aminoimidazole-4-carboxamide ribonucleotide
additional information
additional information
-
Km-values for phosphoribosylanthranilate of mutant enzymes D127V, D127K, D127T, D127G, D127F and D127V/T164H
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0.6
N'-[(5'-phosphoribosyl)formimino]-5-aminoimidazole-4-carboxamide ribonucleotide
-
25°C or 37°C, pH 7.5, wild-type enzyme
1.6
N'-[(5'-phosphoribosyl)formimino]-5-aminoimidazole-4-carboxamide ribonucleotide
-
25°C, pH 7.5
1.8
N'-[(5'-phosphoribosyl)formimino]-5-aminoimidazole-4-carboxamide ribonucleotide
-
37°C, pH 7.5
2
N'-[(5'-phosphoribosyl)formimino]-5-aminoimidazole-4-carboxamide ribonucleotide
-
25°C, pH 7.5, mutant enzyme D127N
2.9
N'-[(5'-phosphoribosyl)formimino]-5-aminoimidazole-4-carboxamide ribonucleotide
-
25°C, pH 7.5, mutant enzyme H48A
5.6
N'-[(5'-phosphoribosyl)formimino]-5-aminoimidazole-4-carboxamide ribonucleotide
-
25°C, pH 7.5, mutant enzyme T164A
6.4
N'-[(5'-phosphoribosyl)formimino]-5-aminoimidazole-4-carboxamide ribonucleotide
-
25°C, pH 7.5, mutant enzyme D51N
37.9
N'-[(5'-phosphoribosyl)formimino]-5-aminoimidazole-4-carboxamide ribonucleotide
-
25°C, pH 7.5, mutant enzyme R83N
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0.00027 - 32
N'-[(5'-phosphoribosyl)formimino]-5-aminoimidazole-4-carboxamide ribonucleotide
additional information
additional information
-
turnover-numbers for phosphoribosylanthranilate of mutant enzymes D127V, D127K, D127T, D127G, D127F and D127V/T164H
-
0.00027
N'-[(5'-phosphoribosyl)formimino]-5-aminoimidazole-4-carboxamide ribonucleotide
-
25°C, pH 7.5, mutant enzyme D127N
0.018
N'-[(5'-phosphoribosyl)formimino]-5-aminoimidazole-4-carboxamide ribonucleotide
-
25°C, pH 7.5, mutant enzyme T164A
0.23
N'-[(5'-phosphoribosyl)formimino]-5-aminoimidazole-4-carboxamide ribonucleotide
-
25°C, pH 7.5, mutant enzyme R83N
0.29
N'-[(5'-phosphoribosyl)formimino]-5-aminoimidazole-4-carboxamide ribonucleotide
-
25°C, pH 7.5, mutant enzyme D51N
0.38
N'-[(5'-phosphoribosyl)formimino]-5-aminoimidazole-4-carboxamide ribonucleotide
-
25°C, pH 7.5, mutant enzyme H48A
0.67
N'-[(5'-phosphoribosyl)formimino]-5-aminoimidazole-4-carboxamide ribonucleotide
-
25°C, pH 7.5, wild-type enzyme
1.54
N'-[(5'-phosphoribosyl)formimino]-5-aminoimidazole-4-carboxamide ribonucleotide
-
37°C, pH 7.5, wild-type enzyme
2.94
N'-[(5'-phosphoribosyl)formimino]-5-aminoimidazole-4-carboxamide ribonucleotide
-
37°C, pH 7.5, wild-type enzyme
4.9
N'-[(5'-phosphoribosyl)formimino]-5-aminoimidazole-4-carboxamide ribonucleotide
-
25°C, pH 7.5
6.08
N'-[(5'-phosphoribosyl)formimino]-5-aminoimidazole-4-carboxamide ribonucleotide
-
25°C, pH 7.5, wild-type enzyme
14.3
N'-[(5'-phosphoribosyl)formimino]-5-aminoimidazole-4-carboxamide ribonucleotide
-
37°C, pH 7.5
32
N'-[(5'-phosphoribosyl)formimino]-5-aminoimidazole-4-carboxamide ribonucleotide
-
80°C, pH 7.5, wild-type enzyme
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D7N
residue D7 acts as the catalytic base, crystallization data
D7N/D176A
residue D7 acts as the catalytic base, and D176 acts as the catalytic acid, crystallization data
D7N
-
residue D7 acts as the catalytic base, crystallization data
-
D7N/D176A
-
residue D7 acts as the catalytic base, and D176 acts as the catalytic acid, crystallization data
-
D127F
-
mutation generates phosphoribosylanthranilate isomerase activity
D127G
-
mutation generates phosphoribosylanthranilate isomerase activity
D127K
-
mutation generates phosphoribosylanthranilate isomerase activity
D127N
-
turnover number for N-[(5'-phosphoribosyl)formimino]-5-aminoimidazole-4-carboxamide ribonucleotide is 2481fold higher than that of the wild-type enzyme. The Km-value for N-[(5'-phosphoribosyl)formimino]-5-aminoimidazole-4-carboxamide ribonucleotide is 3.3fold higher than that of the wild-type enzyme
D127T
-
mutation generates phosphoribosylanthranilate isomerase activity
D127V
-
mutation generates phosphoribosylanthranilate isomerase activity
D127V/T164H
-
mutation generates phosphoribosylanthranilate isomerase activity
D51N
-
turnover number for N-[(5'-phosphoribosyl)formimino]-5-aminoimidazole-4-carboxamide ribonucleotide is 2.3fold higher than that of the wild-type enzyme. The Km-value for N-[(5'-phosphoribosyl)formimino]-5-aminoimidazole-4-carboxamide ribonucleotide is 9.4fold higher than that of the wild-type enzyme
D8N
-
activity with N-[(5'-phosphoribosyl)formimino]-5-aminoimidazole-4-carboxamide ribonucleotide is immeasurable low
H48A
-
turnover number for N-[(5'-phosphoribosyl)formimino]-5-aminoimidazole-4-carboxamide ribonucleotide is 1.8fold lower than that of the wild-type enzyme. The Km-value for N-[(5'-phosphoribosyl)formimino]-5-aminoimidazole-4-carboxamide ribonucleotide is 4.8fold lower than that of the wild-type enzyme
R83N
-
turnover number for N-[(5'-phosphoribosyl)formimino]-5-aminoimidazole-4-carboxamide ribonucleotide is 2.9fold higher than that of the wild-type enzyme. The Km-value for N-[(5'-phosphoribosyl)formimino]-5-aminoimidazole-4-carboxamide ribonucleotide is 63.2fold higher than that of the wild-type enzyme
T164A
-
turnover number for N-[(5'-phosphoribosyl)formimino]-5-aminoimidazole-4-carboxamide ribonucleotide is 37.2fold higher than that of the wild-type enzyme. The Km-value for N-[(5'-phosphoribosyl)formimino]-5-aminoimidazole-4-carboxamide ribonucleotide is 9.3fold higher than that of the wild-type enzyme
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Martin, R.G.; Berberich, M.A.; Ames, B.N.; Davis, W.W.; Goldberger, R.F.; Yourno, J.D.
Enzymes and intermediates of histidine biosynthesis in Salmonella typhimurium
Methods Enzymol.
17B
3-44
1971
Salmonella enterica subsp. enterica serovar Typhimurium
-
brenda
Margolies, M.N.; Goldberger, R.F.
Physical and chemical characterization of the isomerase of histidine biosynthesis in Salmonella typhimurium
J. Biol. Chem.
242
256-264
1967
Salmonella enterica subsp. enterica serovar Typhimurium
brenda
Margolies, M.N.; Goldberger, R.F.
Isolation of the fourth enzyme (isomerase) of histidine biosynthesis from Salmonella typhimurium
J. Biol. Chem.
241
3262-3269
1966
Salmonella enterica subsp. enterica serovar Typhimurium
brenda
Henn-Sax, M.; Thoma, R.; Schmidt, S.; Hennig, M.; Kirschner, K.; Sterner, R.
Two (betaalpha)8-barrel enzymes of histidine and tryptophan biosynthesis have similar reaction mechanisms and common strategies for protecting their labile substrates
Biochemistry
41
12032-12042
2002
Escherichia coli, Thermotoga maritima
brenda
Barona-Gomez, F.; Hodgson, D.A.
Occurrence of a putative ancient-like isomerase involved in histidine and tryptophan biosynthesis
EMBO Rep.
4
296-300
2003
Mycobacterium tuberculosis, Streptomyces coelicolor, Mycobacterium tuberculosis HR37Rv
brenda
Thoma, R.; Obmolova, G.; Lang, D.A.; Schwander, M.; Jeno, P.; Sterner, R.; Wilmanns, M.
Efficient expression, purification and crystallisation of two hyperthermostable enzymes of histidine biosynthesis
FEBS Lett.
454
1-6
1999
Thermotoga maritima
brenda
Fujimori, K.; Tada, S.; Kanai, S.; Ohta, D.
Molecular cloning and characterization of the gene encoding N'-[(5'-phosphoribosyl)-formimino]-5-aminoimidazole-4-carboxamide ribonucleotide (BBM II) isomerase from Arabidopsis thaliana
Mol. Gen. Genet.
259
216-223
1998
Arabidopsis thaliana (O82782), Arabidopsis thaliana
brenda
Leopoldseder, S.; Claren, J.; Jurgens, C.; Sterner, R.
Interconverting the catalytic activities of (betaalpha)(8)-barrel enzymes from different metabolic pathways: sequence requirements and molecular analysis
J. Mol. Biol.
337
871-879
2004
Thermotoga maritima
brenda
Noutoshi, Y.; Ito, T.; Shinozaki, K.
Albino and pale green 10 encodes BBMII isomerase involved in histidine biosynthesis in Arabidopsis thaliana
Plant Cell Physiol.
46
1165-1172
2005
Arabidopsis thaliana (Q5TKS8), Arabidopsis thaliana
brenda
Quevillon-Cheruel, S.; Leulliot, N.; Graille, M.; Blondeau, K.; Janin, J.; van Tilbeurgh, H.
Crystal structure of the yeast His6 enzyme suggests a reaction mechanism
Protein Sci.
15
1516-1521
2006
Saccharomyces cerevisiae (P40545), Saccharomyces cerevisiae
brenda
Koenigsknecht, M.J.; Lambrecht, J.A.; Fenlon, L.A.; Downs, D.M.
Perturbations in histidine biosynthesis uncover robustness in the metabolic network of Salmonella enterica
PLoS ONE
7
e48207
2012
Salmonella enterica, Salmonella enterica DM10374
brenda
Soederholm, A.; Guo, X.; Newton, M.S.; Evans, G.B.; Naesvall, J.; Patrick, W.M.; Selmer, M.
Two-step ligand binding in a (betaalpha)8 barrel enzyme Substrate-bound structures shed new light on the catalytic cycle of HisA
J. Biol. Chem.
290
24657-24668
2015
Salmonella enterica subsp. enterica serovar Typhimurium (P10372), Salmonella enterica subsp. enterica serovar Typhimurium ATCC 700720 (P10372)
brenda