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Information on EC 5.3.1.1 - triose-phosphate isomerase and Organism(s) Arabidopsis thaliana

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Arabidopsis thaliana
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The taxonomic range for the selected organisms is: Arabidopsis thaliana
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea
Synonyms
triosephosphate isomerase, triose phosphate isomerase, triose-phosphate isomerase, tctim, pftim, gltim, monotim, pfutim, cp 25, cytoplasmic tpi, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
CP 25
-
-
-
-
D-glyceraldehyde-3-phosphate ketol-isomerase
-
-
-
-
Isomerase, triose phosphate
-
-
-
-
Lactacin B inducer protein
-
-
-
-
monoTIM
-
-
-
-
PfTIM
-
-
-
-
Phosphotriose isomerase
-
-
-
-
TIM
-
-
-
-
Triose phosphate isomerase
Triose phosphate mutase
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-
-
-
Triose phosphoisomerase
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-
-
-
Triosephosphate isomerase
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-
-
-
Triosephosphate mutase
-
-
-
-
vTIM
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
intramolecular oxidoreduction
-
-
-
-
isomerization
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
D-glyceraldehyde-3-phosphate aldose-ketose-isomerase
-
CAS REGISTRY NUMBER
COMMENTARY hide
9023-78-3
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
D-glyceraldehyde 3-phosphate
dihydroxyacetone 3-phosphate
show the reaction diagram
-
-
-
?
D-Glyceraldehyde 3-phosphate
Glycerone phosphate
show the reaction diagram
-
-
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
D-Glyceraldehyde 3-phosphate
Glycerone phosphate
show the reaction diagram
-
-
-
?
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Diamide
1 mM, presence of glutathione, complete inhibition by S-glutathionylation
glutathione disulfide
modification of residues Cys127 and Cys218
H2O2
0.5 mM, 120 min, 30% loss of activity in presence and absence of glutathione
N-ethylmaleimide
0.5 mM, 60 min, more than 90% loss of activity. Addition of dithiothreitol does not restore activity. In the presence of 1 mM glutathione, the addition of 0.5 mM N-ethylmaleimide does not significantly affect activity
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.48 - 1.68
D-glyceraldehyde 3-phosphate
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
14.8 - 3406
D-glyceraldehyde 3-phosphate
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
29.6 - 2580
D-glyceraldehyde 3-phosphate
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
with plastid TPI isozyme
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
the plastid isoform of triose phosphate isomerase plays a crucial role in this transition from heterotrophic to autotrophic growth
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
TPIS_ARATH
254
0
27169
Swiss-Prot
other Location (Reliability: 2)
TPIC_ARATH
315
0
33346
Swiss-Prot
Chloroplast (Reliability: 2)
Q43279_ARATH
98
0
10577
TrEMBL
Secretory Pathway (Reliability: 4)
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
C127S
loss of more than 97% of specific activity
C127S/C218S
loss of more than 97% of specific activity
C218S
loss of about 21% of specific activity. In presence of glutathione disulfide, mutant behaves similarly to the wild-type, but retains more activity than the wild-type after 240 min of incubation
additional information
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Chen, M.; Thelen, J.J.
The plastid isoform of triose phosphate isomerase is required for the postgerminative transition from heterotrophic to autotrophic growth in Arabidopsis
Plant Cell
22
77-90
2010
Arabidopsis thaliana (P48491), Arabidopsis thaliana
Manually annotated by BRENDA team
Dumont, S.; Bykova, N.; Pelletier, G.; Dorion, S.; Rivoal, J.
Cytosolic triosephosphate isomerase from Arabidopsis thaliana is reversibly modified by glutathione on cysteines 127 and 218
Front. Plant Sci.
7
1942
2016
Arabidopsis thaliana (P48491)
Manually annotated by BRENDA team