Information on EC 4.5.1.1 - DDT-dehydrochlorinase

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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria

EC NUMBER
COMMENTARY
4.5.1.1
-
RECOMMENDED NAME
GeneOntology No.
DDT-dehydrochlorinase
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
1,1,1-trichloro-2,2-bis(4-chlorophenyl)ethane = 1,1-dichloro-2,2-bis(4-chlorophenyl)ethylene + chloride
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
elimination
-
-
of HCl, C-Cl bond cleavage
-
PATHWAY
KEGG Link
MetaCyc Link
DDT degradation
-
Metabolic pathways
-
Microbial metabolism in diverse environments
-
SYSTEMATIC NAME
IUBMB Comments
1,1,1-trichloro-2,2-bis(4-chlorophenyl)ethane chloride-lyase [1,1-dichloro-2,2-bis(4-chlorophenyl)ethylene-forming]
-
SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
DDT dehydrochlorinase
-
-
DDT-ase
-
-
-
-
dehydrochlorinase
-
-
-
-
GSTE2-2
-
isoform
GSTE5-5
-
isoform
CAS REGISTRY NUMBER
COMMENTARY
9031-20-3
-
ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
strains PMD-R
-
-
Manually annotated by BRENDA team
Thai mosquito, glutathione S-transferase isozyme 4c has enzymic activity
-
-
Manually annotated by BRENDA team
glutathion S-transferases with enzymic activity
-
-
Manually annotated by BRENDA team
DDT-restistant housedly
-
-
Manually annotated by BRENDA team
DDT-restistant housedly; strain P2/sel
-
-
Manually annotated by BRENDA team
DDT-restistant housedly; strain RD4
-
-
Manually annotated by BRENDA team
Musca domestica P2/sel
strain P2/sel
-
-
Manually annotated by BRENDA team
Musca domestica RD4
strain RD4
-
-
Manually annotated by BRENDA team
Pseudomonas putida T5
-
-
-
Manually annotated by BRENDA team
Fischer 344 rat
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
1,1,1-trichloro-2,2-bis(4-bromophenyl)ethane
? + HCl
show the reaction diagram
-
-
-
?
1,1,1-trichloro-2,2-bis(4-chlorophenyl)ethane
1,1-dichloro-2,2-bis(4-chlorophenyl)ethylene + HCl
show the reaction diagram
-
-
-
-
1,1,1-trichloro-2,2-bis(4-chlorophenyl)ethane
1,1-dichloro-2,2-bis(4-chlorophenyl)ethylene + HCl
show the reaction diagram
-
-
-
-
1,1,1-trichloro-2,2-bis(4-chlorophenyl)ethane
1,1-dichloro-2,2-bis(4-chlorophenyl)ethylene + HCl
show the reaction diagram
-
-
-
?
1,1,1-trichloro-2,2-bis(4-chlorophenyl)ethane
1,1-dichloro-2,2-bis(4-chlorophenyl)ethylene + HCl
show the reaction diagram
-
-
i.e. 1,1-dichloro-2,2-di-(p-chlorophenyl)ethylene, i.e. p,p'-DDE
?
1,1,1-trichloro-2,2-bis(4-chlorophenyl)ethane
1,1-dichloro-2,2-bis(4-chlorophenyl)ethylene + HCl
show the reaction diagram
-
-
i.e. 1,1-dichloro-2,2-di-(p-chlorophenyl)ethylene, i.e. p,p'-DDE
?
1,1,1-trichloro-2,2-bis(4-chlorophenyl)ethane
1,1-dichloro-2,2-bis(4-chlorophenyl)ethylene + HCl
show the reaction diagram
-
1,1,1-trichloro-2,2-di-(p-chlorophenyl)ethane, i.e. p,p'-DDT
i.e. 1,1-dichloro-2,2-di-(p-chlorophenyl)ethylene, i.e. p,p'-DDE
-
1,1,1-trichloro-2,2-bis(4-chlorophenyl)ethane
1,1-dichloro-2,2-bis(4-chlorophenyl)ethylene + HCl
show the reaction diagram
-
i.e. 1,1,1-trichloro-2,2-bis(p-chlorophenyl)ethane, i.e. DDT in the presence of GSH
i.e. 1,1-dichloro-2,2-bis(p-chlorophenyl)ethylene i.e. DDE
ir
1,1,1-trichloro-2,2-bis(4-chlorophenyl)ethane
1,1-dichloro-2,2-bis(4-chlorophenyl)ethylene + HCl
show the reaction diagram
-
the reaction requires the presence of GSH
-
-
?
1,1,1-trichloro-2,2-bis(4-chlorophenyl)ethane
1,1-dichloro-2,2-bis(4-chlorophenyl)ethylene + HCl
show the reaction diagram
-
the reaction requires the presence of GSH
-
?
1,1,1-trichloro-2,2-bis(4-chlorophenyl)ethane
1,1-dichloro-2,2-bis(4-chlorophenyl)ethylene + HCl
show the reaction diagram
-
the reaction requires the presence of GSH
-
?
1,1,1-trichloro-2,2-bis(4-chlorophenyl)ethane
1,1-dichloro-2,2-bis(4-chlorophenyl)ethylene + HCl
show the reaction diagram
Musca domestica P2/sel
-
-
-
-
1,1,1-trichloro-2,2-bis(4-chlorophenyl)ethane
1,1-dichloro-2,2-bis(4-chlorophenyl)ethylene + HCl
show the reaction diagram
Musca domestica RD4
-
1,1,1-trichloro-2,2-di-(p-chlorophenyl)ethane, i.e. p,p'-DDT
i.e. 1,1-dichloro-2,2-di-(p-chlorophenyl)ethylene, i.e. p,p'-DDE
-
1,1,1-trichloro-2,2-bis(4-chlorophenyl)ethane
?
show the reaction diagram
-
-
-
-
-
1,1,1-trichloro-2,2-bis(4-chlorophenyl)ethane
?
show the reaction diagram
-
-
-
-
-
1,1,1-trichloro-2,2-bis(4-chlorophenyl)ethane
?
show the reaction diagram
Musca domestica P2/sel
-
-
-
-
-
1,1,1-trichloro-2,2-bis(4-chlorophenyl)ethane
?
show the reaction diagram
Musca domestica RD4
-
-
-
-
-
1,1,1-trichloro-2,2-bis(4-fluorophenyl)ethane
? + HCl
show the reaction diagram
-
-
-
?
1,1,1-trichloro-2,2-bis(4-iodophenyl)ethane
? + HCl
show the reaction diagram
-
-
-
?
1,1,1-trichloro-2,2-bis(4-methoxyphenyl)ethane
? + HCl
show the reaction diagram
-
-
-
?
1,1,1-trichloro-2,2-bis(4-tolyl)ethane
? + HCl
show the reaction diagram
-
-
-
?
1,1-dichloro-2,2-bis(4-chlorophenyl)ethane
1-chloro-2,2-bis(4-chlorophenyl)ethylene + HCl
show the reaction diagram
-
i.e. TDE, reaction velocity is 4 times the rate of 1,1,1-trichloro-2,2-bis(4-chlorophenyl)ethane, i.e. DDT
-
?
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
1,1,1-trichloro-2,2-bis(4-chlorophenyl)ethane
?
show the reaction diagram
-
-
-
-
-
1,1,1-trichloro-2,2-bis(4-chlorophenyl)ethane
?
show the reaction diagram
-
-
-
-
-
1,1,1-trichloro-2,2-bis(4-chlorophenyl)ethane
?
show the reaction diagram
Musca domestica P2/sel
-
-
-
-
-
1,1,1-trichloro-2,2-bis(4-chlorophenyl)ethane
?
show the reaction diagram
Musca domestica RD4
-
-
-
-
-
COFACTOR
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
glutathione
-
-
METALS and IONS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
Al3+
-
stimulates at a concentration of 0.1 mM
Ca2+
-
stimulates at a concentration of 0.1 mM
Cu2+
-
stimulates at a concentration of 0.1 mM
Fe2+
-
stimulates at a concentration of 0.1 mM
Mg2+
-
stimulates at a concentration of 0.1 mM
Mn2+
-
stimulates at a concentration of 0.1 mM
Zn2+
-
stimulates at a concentration of 0.1 mM
INHIBITORS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
bromosulfophthalein
-
-
Ethacrynic acid
-
-
guanidine hydrochloride
-
complete inhibition at 1 M
iodoacetate
-
inhibition to a minor degree, completely reversible
p-chloromercuribenzoate
-
i.e. PCMB. 1,1,1-trichloro-2,2-bis(4-chlorophenyl)ethane completely protects the fully aggregated enzyme against PCMB inhibition
permethrin
-
uncompetitive inhibitor of isoform GSTE2-2
SDS
-
78% loss of activity at 5% (w/v)
Urea
-
98% loss of activity at 10 mM
ACTIVATING COMPOUND
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
cysteinylglycine
-
activates
GSH
-
Km: 0.25 mM; required
GSH
-
required
GSH
-
required; stabilizes tetrameric enzyme form
KM VALUE [mM]
KM VALUE [mM] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.21
-
1-chloro-2,4-dinitrobenzene
-
pH 6.5, 22C
TURNOVER NUMBER [1/s]
TURNOVER NUMBER MAXIMUM[1/s]
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.0383
-
1,1,1-trichloro-2,2-bis(4-chlorophenyl)ethane
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
SPECIFIC ACTIVITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
0.149
-
-
pH 6.5, 22C
additional information
-
-
-
additional information
-
-
-
additional information
-
-
anaerobic incubation
pH OPTIMUM
pH MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
MOLECULAR WEIGHT
MOLECULAR WEIGHT MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
120000
-
-
PAGE
SUBUNITS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
tetramer
-
1,1,1-trichloro-2,2-bis(4-chlorophenyl)ethane induces aggregation of the enzyme into the active tetrameric form; 4 * 30000, disc electrophoresis; dissociation of the tetrameric enzyme by mercaptoethanol or dithiothreitol
tetramer
Musca domestica P2/sel
-
1,1,1-trichloro-2,2-bis(4-chlorophenyl)ethane induces aggregation of the enzyme into the active tetrameric form; 4 * 30000, disc electrophoresis; dissociation of the tetrameric enzyme by mercaptoethanol or dithiothreitol
-
trimer
-
the trimeric enzyme is 10% as active as the tetrameric form
trimer
Musca domestica P2/sel
-
the trimeric enzyme is 10% as active as the tetrameric form
-
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
side-chain modification
-
phospholipoprotein
side-chain modification
Musca domestica P2/sel
-
phospholipoprotein
-
pH STABILITY
pH STABILITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
4
6
-
stable between for 6 h
6
12
-
stable between for 48 h at 2C
ORGANIC SOLVENT
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
organic solvents
-
instability of the enzyme to organic solvents at temperatures about -5C
STORAGE STABILITY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
instability of the enzyme to freezing and thawing
-
Purification/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
Cloned/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
APPLICATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
biotechnology
-
positive correlation of enzyme activity and resistance against DDT, non-correlation between enzyme activity and GST activity
biotechnology
-
positive correlation of enzyme activity and resistance against DDT
biotechnology
-
positive correlation of enzyme activity and resistance against DDT, and also between enzyme activity and GST activity