Information on EC 4.5.1.1 - DDT-dehydrochlorinase

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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria

EC NUMBER
COMMENTARY hide
4.5.1.1
-
RECOMMENDED NAME
GeneOntology No.
DDT-dehydrochlorinase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
1,1,1-trichloro-2,2-bis(4-chlorophenyl)ethane = 1,1-dichloro-2,2-bis(4-chlorophenyl)ethylene + chloride
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
elimination
-
-
of HCl, C-Cl bond cleavage
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
DDT degradation
-
-
Metabolic pathways
-
-
Microbial metabolism in diverse environments
-
-
SYSTEMATIC NAME
IUBMB Comments
1,1,1-trichloro-2,2-bis(4-chlorophenyl)ethane chloride-lyase [1,1-dichloro-2,2-bis(4-chlorophenyl)ethylene-forming]
-
CAS REGISTRY NUMBER
COMMENTARY hide
9031-20-3
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
strains PMD-R
-
-
Manually annotated by BRENDA team
Thai mosquito, glutathione S-transferase isozyme 4c has enzymic activity
-
-
Manually annotated by BRENDA team
glutathion S-transferases with enzymic activity
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
strain P2/sel
-
-
Manually annotated by BRENDA team
strain RD4
-
-
Manually annotated by BRENDA team
Fischer 344 rat
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
additional information
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
1,1,1-trichloro-2,2-bis(4-bromophenyl)ethane
? + HCl
show the reaction diagram
-
-
-
?
1,1,1-trichloro-2,2-bis(4-chlorophenyl)ethane
1,1-dichloro-2,2-bis(4-chlorophenyl)ethylene + chloride
show the reaction diagram
1,1,1-trichloro-2,2-bis(4-chlorophenyl)ethane
1,1-dichloro-2,2-bis(4-chlorophenyl)ethylene + HCl
show the reaction diagram
1,1,1-trichloro-2,2-bis(4-chlorophenyl)ethane
?
show the reaction diagram
1,1,1-trichloro-2,2-bis(4-fluorophenyl)ethane
? + HCl
show the reaction diagram
-
-
-
?
1,1,1-trichloro-2,2-bis(4-iodophenyl)ethane
? + HCl
show the reaction diagram
-
-
-
?
1,1,1-trichloro-2,2-bis(4-methoxyphenyl)ethane
? + HCl
show the reaction diagram
-
-
-
?
1,1,1-trichloro-2,2-bis(4-tolyl)ethane
? + HCl
show the reaction diagram
-
-
-
?
1,1-dichloro-2,2-bis(4-chlorophenyl)ethane
1-chloro-2,2-bis(4-chlorophenyl)ethylene + HCl
show the reaction diagram
-
i.e. TDE, reaction velocity is 4 times the rate of 1,1,1-trichloro-2,2-bis(4-chlorophenyl)ethane, i.e. DDT
-
?
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
1,1,1-trichloro-2,2-bis(4-chlorophenyl)ethane
1,1-dichloro-2,2-bis(4-chlorophenyl)ethylene + chloride
show the reaction diagram
1,1,1-trichloro-2,2-bis(4-chlorophenyl)ethane
?
show the reaction diagram
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
glutathione
-
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Al3+
-
stimulates at a concentration of 0.1 mM
Cu2+
-
stimulates at a concentration of 0.1 mM
Fe2+
-
stimulates at a concentration of 0.1 mM
Mn2+
-
stimulates at a concentration of 0.1 mM
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
bromosulfophthalein
-
-
Ethacrynic acid
-
-
guanidine hydrochloride
-
complete inhibition at 1 M
iodoacetate
-
inhibition to a minor degree, completely reversible
p-chloromercuribenzoate
-
i.e. PCMB. 1,1,1-trichloro-2,2-bis(4-chlorophenyl)ethane completely protects the fully aggregated enzyme against PCMB inhibition
permethrin
-
uncompetitive inhibitor of isoform GSTE2-2
SDS
-
78% loss of activity at 5% (w/v)
Urea
-
98% loss of activity at 10 mM
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
cysteinylglycine
-
activates
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.037
1,1,1-trichloro-2,2-bis(4-chlorophenyl)ethane
-
pH 7.0, 37C
0.21
1-chloro-2,4-dinitrobenzene
-
pH 6.5, 22C
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0383 - 0.113
1,1,1-trichloro-2,2-bis(4-chlorophenyl)ethane
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.149
-
pH 6.5, 22C
additional information
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
37000
-
about, native PAGE
120000
-
PAGE
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer
tetramer
trimer
additional information
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
glycoprotein
side-chain modification
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4 - 6
-
stable between for 6 h
34739
6 - 12
-
stable between for 48 h at 2C
34739
7
-
purified native enzyme, 37C, stable for 4-5 h
730796
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
37
-
purified native enzyme, pH 7.0, stable for 4-5 h
ORGANIC SOLVENT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
organic solvents
-
instability of the enzyme to organic solvents at temperatures about -5C
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
instability of the enzyme to freezing and thawing
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
native enzyme from strain T5 to homogeneity
-
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
biotechnology