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EC Tree
IUBMB Comments Also acts on N-glycolylneuraminate glycosides. cf. EC 3.2.1.18 (exo-alpha-sialidase) and EC 3.2.1.129 (endo-alpha-sialidase).
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria
Synonyms
sialidase l, neuraminidase b,
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alpha-(2,3)-specific trans-salidase
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anhydroneuraminidase
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EC 3.2.1.138
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formerly
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neuraminidase, anhydro-
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sialglycoconjugate N-acylneuraminylhydrolase (2,7-cyclizing)
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[N-acetylneuraminic acid]n = 2,7-anhydro-alpha-N-acetylneuraminic acid + [N-acetylneuraminic acid]n-1
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hydrolysis of O-glycosyl bond
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glycoconjugate sialyl-lyase (2,7-cyclizing)
Also acts on N-glycolylneuraminate glycosides. cf. EC 3.2.1.18 (exo-alpha-sialidase) and EC 3.2.1.129 (endo-alpha-sialidase).
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4-methylumbelliferyl-alpha-N-acetylneuraminic acid + H2O
4-methylumbelliferone + 2,7-anhydro-alpha-N-acetylneuraminic acid
4-methylumbelliferyl-alpha-N-acetylneuraminic acid glycoproteins or ganglioside + H2O
2,7-anhydro-alpha-N-acetylneuraminic acid
alpha-(2,3)-sialyl oligosacharides
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human alpha-1 acid glycoprotein + H2O
2,7-anhydro-alpha-N-acetylneuraminic acid + ?
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NanB preferentially cleaves alpha2-3 linked sialic acid from human alpha-1 glycoprotein
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4-methylumbelliferyl-alpha-N-acetylneuraminic acid + H2O
4-methylumbelliferone + 2,7-anhydro-alpha-N-acetylneuraminic acid
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only sialoglycoconjugates, not free alpha-N-acetylneuraminic acid
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4-methylumbelliferyl-alpha-N-acetylneuraminic acid + H2O
4-methylumbelliferone + 2,7-anhydro-alpha-N-acetylneuraminic acid
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strict specificity towards the hydrolysis of NeuAcalpha(2-3)Gal-linkages
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4-methylumbelliferyl-alpha-N-acetylneuraminic acid + H2O
4-methylumbelliferone + 2,7-anhydro-alpha-N-acetylneuraminic acid
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strict specificity towards the hydrolysis of NeuAcalpha(2-3)Gal-linkages
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4-methylumbelliferyl-alpha-N-acetylneuraminic acid glycoproteins or ganglioside + H2O
2,7-anhydro-alpha-N-acetylneuraminic acid
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4-methylumbelliferyl-alpha-N-acetylneuraminic acid glycoproteins or ganglioside + H2O
2,7-anhydro-alpha-N-acetylneuraminic acid
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4-methylumbelliferyl-alpha-N-acetylneuraminic acid glycoproteins or ganglioside + H2O
2,7-anhydro-alpha-N-acetylneuraminic acid
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4-methylumbelliferyl-alpha-N-acetylneuraminic acid glycoproteins or ganglioside + H2O
2,7-anhydro-alpha-N-acetylneuraminic acid
4-methylumbelliferyl-alpha-N-acetylneuraminic acid glycoproteins or ganglioside + H2O
2,7-anhydro-alpha-N-acetylneuraminic acid
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4-methylumbelliferyl-alpha-N-acetylneuraminic acid glycoproteins or ganglioside + H2O
2,7-anhydro-alpha-N-acetylneuraminic acid
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4-methylumbelliferyl-alpha-N-acetylneuraminic acid glycoproteins or ganglioside + H2O
2,7-anhydro-alpha-N-acetylneuraminic acid
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additional information
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not: 2-deoxy-2,3-dehydroacetylneuraminic acid, EDTA, Ca2+, Mg2+, Mn2+
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Infections
Pneumococcal neuraminidases A and B both have essential roles during infection of the respiratory tract and sepsis.
Respiratory Tract Infections
Pneumococcal neuraminidases A and B both have essential roles during infection of the respiratory tract and sepsis.
Sepsis
Pneumococcal neuraminidases A and B both have essential roles during infection of the respiratory tract and sepsis.
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0.68
4-methylumbelliferyl-alpha-N-acetylneuraminic acid
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pH 5.5, 37°C
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additional information
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brenda
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brenda
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NANL_MACDE
762
0
82982
Swiss-Prot
Secretory Pathway (Reliability: 1 )
C5WHM3_STRDG
Streptococcus dysgalactiae subsp. equisimilis (strain GGS_124)
131
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14964
TrEMBL
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A0A380KDS1_STREQ
131
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14974
TrEMBL
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NANB_STRPN
Streptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4)
697
1
77695
Swiss-Prot
Secretory Pathway (Reliability: 1 )
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84000
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x * 84000, SDS-PAGE
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crystal structure of NanB in complex with its reaction product 2,7-anhydro-Neu5Ac is reported. NanB crystal structure consists of an N-terminal lectin-like domain (L-domain, residues 30-227), a catalytic six-bladed beta-propeller domain (N-domain, residues 228-345 and 457-697), and an irregular beta-stranded domain inserted into the catalytic domain (I-domain, residues 346-456)
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50% loss of activity after one freeze and thawing cycle, in presence of 10% glycerol stable to several freeze and thawing cycles
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-20°C, 10% glycerol, stable for more than 4 months
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expression in Escherichia coli
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NanB sequence lacking the first 29 residues is PCR-amplified from Streptococcus pneumoniae DNA, cloned into the pOPINF vector and expressed in Escherichia coli
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synthesis
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synthesis of sialyl oligosaccharides using enzyme, since it is difficult to obtain them from natural sources
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Chou, M.Y.; Li, S.C.; Kiso, M.; Hasegawa, A.; Li, Y.T.
Purification and characterization of sialidase L, a NeuAc alpha(2-3)Gal-specific sialidase
J. Biol. Chem.
269
18821-18826
1994
Macrobdella decora
brenda
Li, Y.T.; Nakagawa, H.; Ross, S.A.; Hansson, G.C.; Li, S.C.
A novel sialidase which releases 2,7-anhydro-alpha-N-acetylneuraminic acid from sialoglycoconjugates
J. Biol. Chem.
265
21629-21633
1990
Macrobdella decora
brenda
Luo, Y.; Chou, M.; Li, S.; Li, Y.; Luo, M.
Crystallization and preliminary X-ray studies of sialidase L from the leech Macrobdella decora
Acta Crystallogr. Sect. D
54
111-113
1998
Macrobdella decora
brenda
Takahashi, N.; Lee, K.B.; Nakagawa, H.; Tsukamoto, Y.; Kawamura, Y.; Li, Y.T.; Lee, Y.C.
Enzymatic sialylation of N-linked oligosaccharides using an alpha-(2,3)-specific trans-sialidase from Trypanosoma cruzi: structural identification using a three-dimensional elution mapping technique
Anal. Biochem.
230
333-342
1995
Trypanosoma cruzi
brenda
Gut, H.; King, S.J.; Walsh, M.A.
Structural and functional studies of Streptococcus pneumoniae neuraminidase B: An intramolecular trans-sialidase
FEBS Lett.
582
3348-3352
2008
Streptococcus pneumoniae
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