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Information on EC 4.2.1.17 - enoyl-CoA hydratase and Organism(s) Sus scrofa

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EC Tree
     4 Lyases
         4.2 Carbon-oxygen lyases
             4.2.1 Hydro-lyases
                4.2.1.17 enoyl-CoA hydratase
IUBMB Comments
Acts in the reverse direction. With cis-compounds, yields (3R)-3-hydroxyacyl-CoA. cf. EC 4.2.1.74 long-chain-enoyl-CoA hydratase.
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This record set is specific for:
Sus scrofa
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Word Map
The taxonomic range for the selected organisms is: Sus scrofa
The enzyme appears in selected viruses and cellular organisms
Synonyms
enoyl-coa hydratase, echs1, peroxisomal bifunctional enzyme, 2-enoyl-coa hydratase, amech, short-chain enoyl-coa hydratase, enoyl-coa hydratase/isomerase, beta-hydroxyacyl-coa dehydrase, enoyl-coenzyme a hydratase, fadb', more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
2-enoyl-CoA hydratase
-
-
-
-
2-octenoyl coenzyme A hydrase
-
-
-
-
acyl coenzyme A hydrase
-
-
-
-
beta-hydroxyacid dehydrase
-
-
-
-
beta-hydroxyacyl-CoA dehydrase
-
-
-
-
crotonase
crotonyl hydrase
-
-
-
-
D-3-hydroxyacyl-CoA dehydratase
-
-
-
-
ECH
-
-
-
-
enol-CoA hydratase
-
-
-
-
Enoyl coenzyme A hydrase (D)
-
-
-
-
enoyl coenzyme A hydrase (L)
-
-
-
-
enoyl coenzyme A hydratase
-
-
-
-
enoyl hydrase
-
-
-
-
hydratase, enoyl coenzyme A
-
-
-
-
SCEH
-
-
-
-
short chain enoyl coenzyme A hydratase
-
-
-
-
short-chain enoyl-CoA hydratase
-
-
-
-
trans-2-enoyl-CoA hydratase
-
-
-
-
unsaturated acyl-CoA hydratase
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
elimination
-
-
-
-
hydration
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
(3S)-3-hydroxyacyl-CoA hydro-lyase
Acts in the reverse direction. With cis-compounds, yields (3R)-3-hydroxyacyl-CoA. cf. EC 4.2.1.74 long-chain-enoyl-CoA hydratase.
CAS REGISTRY NUMBER
COMMENTARY hide
9027-13-8
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
crotonyl-CoA + H2O
?
show the reaction diagram
dec-2-enoyl-CoA + H2O
?
show the reaction diagram
-
32% of the activity with crotonyl-CoA. The classification is ambiguous because the stereochemistry is not exactly determined
-
-
?
dodec-2-enoyl-CoA + H2O
?
show the reaction diagram
-
9.6% of the activity with crotonyl-CoA. The classification is ambiguous because the stereochemistry is not exactly determined
-
-
?
hex-2-enoyl-CoA + H2O
?
show the reaction diagram
-
77% of the activity with crotonyl-CoA. The classification is ambiguous because the stereochemistry is not exactly determined
-
-
?
hexadec-2-enoyl-CoA + H2O
?
show the reaction diagram
-
2.4% of the activity with crotonyl-CoA. The classification is ambiguous because the stereochemistry is not exactly determined
-
-
?
oct-2-enoyl-CoA + H2O
?
show the reaction diagram
-
54% of the activity with crotonyl-CoA. The classification is ambiguous because the stereochemistry is not exactly determined
-
-
?
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
acetoacetyl-CoA
-
-
iodoacetamide
-
10 mM, 20 min, 20% inhibition
NEM
-
10 mM, 19% inhibition. 5 mM, 13% inhibition
p-chloromercuribenzoate
-
1 mM, complete inhibition. 0.1 mM, 11% inhibition
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.013
crotonyl-CoA
-
pH 9.0
0.029
dec-2-enoyl-CoA
-
pH 9.0
0.03
dodec-2-enoyl-CoA
-
pH 9.0
0.029
hex-2-enoyl-CoA
-
pH 9.0
0.03
hexadec-2-enoyl-CoA
-
pH 9.0
0.029
oct-2-enoyl-CoA
-
pH 9.0
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.014
acetoacetyl-CoA
-
pH 8.0, due to the binding of the enolate form of acetoacetyl-CoA the Ki-value should be highly pH-dependent
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
1334
-
-
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
8.5
-
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
The classification is ambiguous because the stereochemistry of the reaction product is not exactly determined
-
-
Manually annotated by BRENDA team
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
-
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
ECHA_PIG
763
0
83107
Swiss-Prot
Mitochondrion (Reliability: 1)
A0A8D0LZ91_PIG
933
0
101235
TrEMBL
other Location (Reliability: 1)
A0A8D1D1G7_PIG
723
0
78944
TrEMBL
Mitochondrion (Reliability: 1)
A0A4X1UHY1_PIG
731
0
80317
TrEMBL
other Location (Reliability: 5)
A0A8D1AYA3_PIG
722
0
78643
TrEMBL
Mitochondrion (Reliability: 1)
A0A8D1VRA8_PIG
731
0
80333
TrEMBL
other Location (Reliability: 5)
A0A480JQP2_PIG
723
0
79679
TrEMBL
Mitochondrion (Reliability: 4)
B2ZF49_PIG
763
0
83250
TrEMBL
Mitochondrion (Reliability: 1)
A0A8D1FVD8_PIG
933
0
101152
TrEMBL
other Location (Reliability: 1)
A0A8D1CYW7_PIG
666
0
73368
TrEMBL
Mitochondrion (Reliability: 4)
A0A8D1MHE7_PIG
933
0
101201
TrEMBL
other Location (Reliability: 1)
A0A8D1AN84_PIG
745
0
80804
TrEMBL
Mitochondrion (Reliability: 1)
A0A8D1VVX9_PIG
933
0
101205
TrEMBL
other Location (Reliability: 1)
A0A8D1GPT9_PIG
722
0
78615
TrEMBL
Mitochondrion (Reliability: 1)
A0A5G2QS72_PIG
688
0
75794
TrEMBL
Mitochondrion (Reliability: 4)
A0A8D1XT72_PIG
763
0
83135
TrEMBL
Mitochondrion (Reliability: 1)
A0A4X1UJ95_PIG
723
0
79679
TrEMBL
Mitochondrion (Reliability: 4)
A0A287ACP9_PIG
668
0
74092
TrEMBL
Mitochondrion (Reliability: 4)
A0A8D0I458_PIG
706
0
77846
TrEMBL
Mitochondrion (Reliability: 4)
I3LJ48_PIG
731
0
80317
TrEMBL
other Location (Reliability: 5)
A0A8D0X4B2_PIG
933
0
101219
TrEMBL
other Location (Reliability: 1)
A0A8D0WGV7_PIG
668
0
74092
TrEMBL
Mitochondrion (Reliability: 4)
A0A8D1KJX2_PIG
745
0
80776
TrEMBL
Mitochondrion (Reliability: 1)
A0A5G2RBW4_PIG
734
0
80044
TrEMBL
Mitochondrion (Reliability: 1)
A0A5G2R3M6_PIG
722
0
78615
TrEMBL
Mitochondrion (Reliability: 1)
A0A8D1AN93_PIG
728
0
79467
TrEMBL
Mitochondrion (Reliability: 1)
A0A8D1GJ78_PIG
763
0
83107
TrEMBL
Mitochondrion (Reliability: 1)
A0A8D1D1G3_PIG
867
0
94772
TrEMBL
Mitochondrion (Reliability: 1)
A0A4X1TSV0_PIG
723
0
78916
TrEMBL
Mitochondrion (Reliability: 1)
A0A480VWY5_PIG
763
0
83121
TrEMBL
Mitochondrion (Reliability: 1)
A0A8D1D1T6_PIG
1122
0
125200
TrEMBL
Mitochondrion (Reliability: 1)
A0A8D1CAQ6_PIG
745
0
80790
TrEMBL
Mitochondrion (Reliability: 1)
A0A480PE19_PIG
696
0
75656
TrEMBL
Mitochondrion (Reliability: 4)
A0A8D0SNN1_PIG
1122
0
125143
TrEMBL
Mitochondrion (Reliability: 1)
A0A8D1Q715_PIG
722
0
78615
TrEMBL
Mitochondrion (Reliability: 1)
A0A8D1ADI9_PIG
718
0
79128
TrEMBL
Mitochondrion (Reliability: 4)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
155000
-
non-denaturing PAGE
27300
-
6 * 27300, SDS-PAGE
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hexamer
-
6 * 27300, SDS-PAGE
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Fong, J.C.; Schulz, H.
Purification and properties of pig heart crotonase and the presence of short chain and long chain enoyl coenzyme A hydratases in pig and guinea pig tissues
J. Biol. Chem.
252
542-547
1977
Sus scrofa
Manually annotated by BRENDA team
Sumegi, B.; Srere, P.A.
Binding of the enzymes of fatty acid beta-oxidation and some related enzymes to pig heart inner mitochondrial membrane
J. Biol. Chem.
259
8748-8752
1984
Sus scrofa
Manually annotated by BRENDA team