Information on EC 4.1.2.43 - 3-hexulose-6-phosphate synthase

D-ribulose 5-phosphate + acetaldehyde

?

D-ribulose 5-phosphate + benzaldehyde

?

D-ribulose 5-phosphate + bromoacetaldehyde

?

D-ribulose 5-phosphate + butyraldehyde

?

-

-

?

D-ribulose 5-phosphate + chloral

?

-

-

?

D-ribulose 5-phosphate + chloroacetaldehyde

?

-

-

?

D-ribulose 5-phosphate + chloropropionaldehyde

?

-

-

?

D-ribulose 5-phosphate + dichloroacetaldehyde

?

-

-

?

D-ribulose 5-phosphate + DL-glyceraldehyde

?

-

-

?

D-ribulose 5-phosphate + formaldehyde

D-arabino-3-hexulose 6-phosphate

21 entries

D-ribulose 5-phosphate + formaldehyde

D-arabino-hex-3-ulose 6-phosphate

40 entries

D-ribulose 5-phosphate + glutaraldehyde

?

-

-

?

D-ribulose 5-phosphate + glycolaldehyde

4-heptulose 7-phosphate

D-ribulose 5-phosphate + glycolaldehyde

?

D-ribulose 5-phosphate + glyoxal

?

-

-

?

D-ribulose 5-phosphate + isobutyraldehyde

?

-

-

?

D-ribulose 5-phosphate + isovaleraldehyde

?

-

-

?

D-ribulose 5-phosphate + methylglyoxal

?

D-ribulose 5-phosphate + methylmercaptopropionaldehyde

?

-

-

?

D-ribulose 5-phosphate + propionaldehyde

?

-

-

?

D-ribulose 5-phosphate + pyridine-2-carboxaldehyde

?

-

-

?

D-ribulose 5-phosphate + pyridine-3-carboxaldehyde

?

-

-

?

D-ribulose 5-phosphate + pyridine-4-carboxaldehyde

?

-

-

?

D-ribulose 5-phosphate + valeraldehyde

?

-

-

?

additional information

?

-

10 entries

D-arabino-hex-3-ulose 6-phosphate

D-ribulose 5-phosphate + formaldehyde

D-ribulose 5-phosphate + formaldehyde

D-arabino-3-hexulose 6-phosphate

21 entries

D-ribulose 5-phosphate + formaldehyde

D-arabino-hex-3-ulose 6-phosphate

6 entries

additional information

?

-

Ca2+

Cd2+

-

the enzyme activity is also promoted with 1 mM Cd2+

Co2+

Cu2+

-

33% stimulation of activity at 1 mM

Fe2+

Mg2+

Mn2+

Ni2+

-

97% stimulation of activity at 1 mM

sulfate

the active site of each HPS monomer contains a sulfate ion

Zn2+

ADP

-

in the presence of 1 mM ADP, the enzyme retains 37% activity

AMP

-

complete inhibition at 1 mM AMP

ATP

-

in the presence of 1 mM ATP, the enzyme retains 86% activity

Ca2+

-

49% inhibition at 1 mM

Cu2+

D-ribulose 5-phosphate

-

commercial D-ribulose 5-phosphate inhibits the enzyme

EDTA

Glutaraldehyde

-

22% inhibition at 4 mM

glyceraldehyde

-

13% inhibition at 4 mM

glycolaldehyde

-

49% inhibition at 4 mM

glycolic acid

-

glycolic acid inhibits the enzyme competitively with respect to formaldehyde

690311

glyoxylate

-

12% inhibition at 4 mM

Hg2+

-

81% inhibition with 1 mM Hg2+

methylglyoxal

-

26% inhibition at 4 mM

Ni2+

-

64% inhibition at 1 mM

o-phenanthroline

-

the enzyme is completely inhibited by the presence of 2 mM o-phenanthroline

Pb2+

-

48% inhibition with 1 mM Pb2+

phosphate

-

in the presence of 1 mM phosphate, the enzyme retains 95% activity

Tiron

-

the enzyme is completely inhibited by the presence of 2 mM tiron

additional information

-

formaldehyde

Bacillus subtilis

-

expression of the HPS coding hxlAB operon is induced by the presence of formaldehyde

694212

HxlR

Bacillus subtilis

-

necessary for formaldehyde-induced expression of the HPS coding hxlAB operon

694212

-

0.075

D-arabino-hex-3-ulose 6-phosphate

-

in 50 mM sodium potassium phosphate buffer, 5 mM MgCl2, at pH 7.0, at 30°C

0.0045 - 1.6

D-ribulose 5-phosphate

16 entries

0.00147 - 2.96

formaldehyde

20 entries

4.3

glycolaldehyde

5.7

methylglyoxal

58.8

D-ribulose 5-phosphate

-

at pH 8.5 and 30°C

45.82 - 159

formaldehyde

452.3

D-ribulose 5-phosphate

-

at pH 8.5 and 30°C

46.8

formaldehyde

-

at pH 8.5 and 30°C

1.4

-

cell free extract, at 30°C

1.71

-

cell free extract, at 30°C

148

-

after 15.1fold purification

687371

20

purified enzyme

235

-

after 4.14fold purification

26

-

crude extract

3.2

-

cell free extract, at 30°C

690311, 694004

3.3

-

cell free extract

3.5

-

cell free extract, at 50°C

4.4

-

hexulose-6-phosphate synthase activity of the gene product Fae-Hps, at 30°C, pH 7.0

690639

4.5

-

crude extract, at 30°C

694012

41.1

-

cell free extract, recombinant HPS

4100

-

after 161fold purification

52

-

after 16fold purification

56.7

-

crude cell extract

64

66.5

-

after 14.8fold purification, at 30°C

694012

69

-

after 41fold purification, at 30°C

70.9

-

after 1.73fold purification, recombinant HPS

74.2

-

after 23fold purification, at 30°C

690311, 694004

9.8

-

cell free extract

687371

7

7 - 8

-

7.5 - 8

8

-

9.5

-

30

-

40

-

purified recombinant enzyme

45

-

50

-

60

-

30 - 50

-

the enzyme activity is 70% higher at 50°C than at 30°C

5.1

-

isoelectric focusing

Acetobacter sp.

-

690225, 690226

-

Acetobacter sp. MB 58

-

690225, 690226

-

-

Acidomonas methanolica MB58

-

-

-

-

UniProt

Aminomonas aminovorus C2A1

-

Bacillus methanolicus MGA3

UniProt

Arthrobacter globiformis

-

695279

-

Bacillus methanolicus

-

701850, 743689

-

-

701850, 743689

-

-

691361, 694003, 701850

-

Bacillus sp. (in: Bacteria) C1

-

691361, 694003

-

Bacillus subtilis

-

694212, 701850, 746827

-

Brevibacillus brevis

-

-

Brevibacillus brevis S1

-

Methanocaldococcus jannaschii

-

-

-

Methanococcus

-

-

Methylobacillus flagellatus

-

690639, 691361

-

Methanosarcina barkeri Fusaro / DSM 804

-

-

-

-

-

-

obligate methylotroph

-

Methylomonas aminofaciens 77a

Methylophilus methylotrophus

-

690565, 691361, 694012, 695279

-

Methylomonas sp. GBS

-

695279

-

Methylomonas sp. GI33

-

695279

-

Methylomonas sp. M15 / DSM 580

-

690565, 691361, 694012, 695279

-

-

691361

-

-

Methylotuvimicrobium alcaliphilum 20Z

-

-

-

Pseudomonas oleovorans

-

-

-

-

-

-

-

-

Pyrococcus horikoshii OT-3

strain OT3

-

-

-

-

-

-

-

-

687371, 701850

-

evolution

21 entries

physiological function

-

bifunctional enzyme

3ajx, download, 3D-view

162000

-

EDTA-solubilized HPS-PHI fusion enzyme, gel filtration

20400

-

4 * 20400, SDS-PAGE

22000

Methylophilus methylotrophus

22500

-

2 * 22500

691361

23000

24000

25000

-

recombinant HPS, gel filtration

27000

310000

32000

40000

42000

-

SDS-PAGE

43000

44000

45000

46000

holoenzyme

47000

48000

-

2 * 48000, SDS-PAGE

49000

80000

-

gel filtration

dimer

homodimer

homohexamer

homotetramer

monomer

additional information

Pseudomonas oleovorans

-

multiple forms of the enzyme may be responsible for the generation of the complex shape, and thus, the kinetic characteristic should he the sum of differing characteristics

695278

in complex with a sulfate ion, sitting drop vapor diffusion method, using 18% (w/v) polyethylene glycol (PEG) 3350 and 0.2 M MgCl2

H134A

the mutation abolishes HPS activity

H134D

the mutation abolishes HPS activity

H134K

the mutation abolishes HPS activity

H134N

the mutation abolishes HPS activity

H134S

the mutation abolishes HPS activity

K61A

the mutant exhibits 50-90% of the activity of the wild type enzyme

K61D

the mutant exhibits 50-90% of the activity of the wild type enzyme

K61H

the mutant exhibits 50-90% of the activity of the wild type enzyme

K61S

the mutant exhibits 50-90% of the activity of the wild type enzyme

H134A

-

the mutation abolishes HPS activity

-

H134D

-

the mutation abolishes HPS activity

-

H134K

-

the mutation abolishes HPS activity

-

H134N

-

the mutation abolishes HPS activity

-

K61A

-

the mutant exhibits 50-90% of the activity of the wild type enzyme

-

additional information

6 entries

6 - 8

-

remains stable at pH 6.0-8.0 at 30°C for 1 h, enzyme stability decreases at pH values below 6.0

694012

7 - 8

-

stable in neutral to slightly alkaline solutions

691118

40 - 60

-

no decrease in activity is observed within 6 h at 40°C in the presence of 5 mM MgCl2, at 50°C and 60°C the enzyme is stable for 2.5 and 0.5 h, respectively, one-half the initial activities are found after 6 and 1.5 h respectively

40 - 80

-

almost full activity is retained after incubation at 40°C for 30 min, whereas at 80°C for 10 min the enzyme is completely inactivated

55 - 65

-

the enzyme retains more than 50% of its activity at 55°C after 1 h, the enzyme retains more than 50% of its activity at 65°C after 30 min

6.5 - 7.5

-

remains stable in the pH range of 6.5-7.5 at 30°C for 6 h

60

-

3-hexulose phosphate synthase is rapidly inactivated at elevated temperatures, activity is totally lost within 5 min at 60°C

90

9 entries

-15°C, in the presence of 2.5 mM MgCl2, 6 months, remains stable unless repeatedly frozen and thawed

-

-20°C, purified enzyme, at least 1 year, no loss of activity

-

-80°C, purified enzyme, in the presence of 5 mM MgSO4, 5 mM D-ribose 5-phosphate, and 1.75 units/ml phosphoriboisomerase, at least 5 months, no loss of activity

-

0°C to -4°C, purified enzyme in the presence of 3.2 M ammonium sulfate, at least 1 year, no loss of activity

-

-

ammonium sulfate fractionation, DEAE-cellulose column chromatography, and Sephadex G-100 gel filtration

-

ammonium sulfate precipitation, DEAE-cellulose column chromatography

-

DEAE-cellulose column chromatography, DEAE-Sephadex A-50 gel filtration, and Sephadex G-75 gel filtration

-

694012

DEAE-cellulose column chromatography, hydroxylapatite column chromatography, Sephadex G-150 gel filtration, Sephadex G-100 gel filtration, and DEAE-Sephadex A-50 gel filtration

-

DEAE-Sephacel column chromatography, Q-Sepharose column chromatography, and hydroxyapatite column chromatography

-

690639

DEAE-Toyopearl column chromatography butyl-Toyopearl column chromatography

-

DEAE-Toyopearl column chromatography, butyl-Toyopearl column chromatography, and ammonium sulfate precipitation

-

Ni-chelating Sepharose column chromatography

Ni-NTA column chromatography

-

phenyl-Sepharose column chromatography and DEAE-Sephacel column chromatography

-

690311

phenyl-Sepharose column chromatography, and DEAE-Sephacel column chromatography

-

polyethylene imine precipitation, S-Sepharose column chromatography, Q-Sepharose column chromatography, and Sephadex G-75 gel filtration

-

Q-Sepharose column chromatography, phenyl-Superose column chromatography, Superose 12 chromatography, and Sephadex G-25 gel filtration

-

ultracentrifugation

-

687371

3-hexulose-6-phosphate synthase/6-phosphate-3-hexuloisomerase fusion enzyme from Mycobacterium gastri is expressed in chloroplasts of Pelargonium sp.

-

714569

expressed as fusion protein with 6-phospho-3-hexulose isomerase in Escherichia coli (DE3) Rosetta cells

-

expressed in Corynebacterium glutamicum

expressed in Escherichia coli

expressed in Escherichia coli BL21 (DE3) cells

expressed in Escherichia coli BL21 (DE3) pLysS cells

-

690639

expressed in Escherichia coli Rosetta (DE3) cells

expressed in Escherichia coli Rosetta (DE3) cells, HPS only, or as fusion enzyme with 6-phospho-3-hexuloisomerase

-

expressed in Escherichia coli Rosetta(DE3) cells

expression in Escherichia coli

environmental protection

synthesis