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Information on EC 3.6.4.6 - vesicle-fusing ATPase and Organism(s) Mus musculus

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IUBMB Comments
A large family of ATP-hydrolysing enzymes involved in the heterotypic fusion of membrane vesicles with target membranes and the homotypic fusion of various membrane compartments. They belong to the AAA-type (_A_TPase _a_ssociated with a variety of cell _a_ctivities) ATPase superfamily. They include peroxin, which apparently is involved in Zellweger's syndrome.
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Mus musculus
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The taxonomic range for the selected organisms is: Mus musculus
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria, Archaea
Reaction Schemes
hide
ATP
+
H2O
=
ADP
+
phosphate
+
=
+
Synonyms
n-ethylmaleimide-sensitive factor, cdc48, aaa-atpase, n-ethylmaleimide-sensitive fusion protein, atp6v0d2, n-ethylmaleimide sensitive factor, sec18p, sec18, cdc48p, pex1p, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ATP phosphohydrolase
-
-
ATP6V0D1
-
subunit
ATP6V0D2
-
subunit
ATPase N-ethylmaleimide sensitive factor
-
-
N-ethylmaleimide sensitive fusion protein
-
-
-
-
N-ethylmaleimide sensitive-factor
-
-
N-ethylmaleimide-sensitive factor
-
-
N-ethylmaleimide-sensitive fusion protein
-
-
NEM-sensitive fusion protein
-
-
-
-
SKD1/VPS4B
-
SKD2 protein
-
-
-
-
suppresor of potassium transport growth defect
-
-
V-ATPase
-
-
vacular protein sorting 4 B
-
-
vesicle-fusing ATPase
-
-
Vesicular-fusion protein NSF
-
-
-
-
Vps 4B
-
-
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
ATP + H2O = ADP + phosphate
show the reaction diagram
large family of ATP-hydrolysing enzymes involved in the heterotypic fusion of membrane vesicles with target membranes and the homotypic fusion of various membrane compartments. They belong to the AAA-type (ATPase associated with a variety of cell activities) ATPase superfamily. They include peroxin, which apparently is involved in Zellweger 's syndrome.
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of phosphoric ester
SYSTEMATIC NAME
IUBMB Comments
ATP phosphohydrolase (vesicle-fusing)
A large family of ATP-hydrolysing enzymes involved in the heterotypic fusion of membrane vesicles with target membranes and the homotypic fusion of various membrane compartments. They belong to the AAA-type (_A_TPase _a_ssociated with a variety of cell _a_ctivities) ATPase superfamily. They include peroxin, which apparently is involved in Zellweger's syndrome.
CAS REGISTRY NUMBER
COMMENTARY hide
9000-83-3
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ATP + H2O
ADP + phosphate
show the reaction diagram
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ATP + H2O
ADP + phosphate
show the reaction diagram
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
bafilomycin A
-
-
TAT-NSF700
-
-
-
additional information
-
phosphorylation by Pctaire1 attenuates the hexamerization of NSF
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
-
SKD1 E Vps proteins SBP1 and mVps2 interact with SKD1
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
modest expression
Manually annotated by BRENDA team
-
MIN-6 beta cell
Manually annotated by BRENDA team
additional information
-
not expressed in lymphoid cells
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
-
localizes mainly in acrosome. ATPase N-ethylmaleimide sensitive factor may be a common feature in the triggering/regulation of membrane merging during mammalian acrosome reaction
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
-
subunit Atp6v0d2-deficient macrophages have augmented mitochondrial damage, enhanced inflammasome activation and reduced clearance of Salmonella typhimurium
physiological function
-
subunit ATP6V0D2 is a key component of macrophage-specific autophagosome-lysosome fusion machinery maintaining macrophage organelle homeostasis and, in turn, limiting both inflammation and bacterial infection. The enzyme subunit Atp6v0d2 inhibits inflammasome-dependent colitis. Subunit ATP6V0D1 but not ATP6V0D2 is required for lysosome acidification and maturation in macrophages. Subunit ATP6V0D2, but not ATP6V0D1, forms a complex with VAMP8 and STX17, and is required for the optimal stabilization of this complex in activated macrophages
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
VPS4A_MOUSE
437
0
48907
Swiss-Prot
other Location (Reliability: 1)
NSF_MOUSE
744
0
82613
Swiss-Prot
other Location (Reliability: 3)
TERA_MOUSE
806
0
89322
Swiss-Prot
other Location (Reliability: 1)
VPS4B_MOUSE
444
0
49419
Swiss-Prot
other Location (Reliability: 2)
G3UX98_MOUSE
76
0
8446
TrEMBL
other Location (Reliability: 2)
A0A0A0MQ80_MOUSE
892
0
97157
TrEMBL
Mitochondrion (Reliability: 5)
Q3TDX2_MOUSE
437
0
48835
TrEMBL
other Location (Reliability: 1)
Q3U8P5_MOUSE
444
0
49464
TrEMBL
other Location (Reliability: 2)
G3UX86_MOUSE
210
0
23541
TrEMBL
Mitochondrion (Reliability: 4)
Q3TN07_MOUSE
444
0
49419
TrEMBL
other Location (Reliability: 2)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
148000
ATP-bound form, Guinier analysis
39000
apo-form, Guinier analysis
50000
ADP-bound form, Guinier analysis
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
homohexamer
-
-
additional information
addition of ATP but not ADP increases the oligomerized population of SKD1
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
phosphoprotein
-
phosphorylated by Pctaire1 on serine 569
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
hanging drop vapour diffusion method, using 5% (w/v) polyethylene glycol 3350 and 100 mM MES (pH 6.0)
hanging-drop vapour-diffusion method. Preparation of crystals of the N-terminal domain of PEX1. The crystals belong to space group P3(1) or P3(2) with unit-cell parameters a = b = 63.5 A, c = 33.5 A, and contain one protein molecule per crystallographic asymmetric unit. 2.05 A resolution
-
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
E235Q
-
no ATPase activity, mutation leads to perturbation of various membrane transports via endosomes
E305Q
-
mutant assembles into oligomer like wild-type, similar ATPase activity as wild-type
E305Q/E578Q
-
no ATPase activity
E578Q
-
mutant assembles into oligomer like wild-type, severely impaired ATPase activity
S569A
-
mutation of serine 569 to alanine abolishs the phosphorylation of D2 domain by active Pctaire1
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
Ni-NTA column chromatography, DEAE Sepharose column chromatography, and Superdex 75 gel filtration
recombinant NSF is purified by nickel-nitrilotriacetic acid-agarose affinity chromatography
-
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli BL21(DE3) pLysE cells
expression in Escherichia coli
-
expression in rat 3Y1B fibroblast cells
-
full-length, partial cDNA fragments and point mutations of mouse NSF are amplified by PCR and subcloned into different expression vectors including pGEX-6P-2, pETH-32, pcDNA3-His6, and pcDNA3-3HA
-
N-terminal domain of PEX1
-
SKD1 cDNA amplified by reverse transcription-PCR
-
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
thr expression of subunit ATP6V0D2 is most inhibited by the addition of lipopolysaccharide
-
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Confalonieri, F.; Duguet, M.
A 200-amino acid ATPase module in search of a basic function
Bioessays
17
639-650
1995
Saccharomyces cerevisiae, Candida albicans, Cricetulus longicaudatus, Drosophila melanogaster, Homo sapiens, Mus musculus, Sulfolobus acidocaldarius, Xenopus laevis
Manually annotated by BRENDA team
Finken-Eigen, M.; Roehricht, R.A.; Koehrer, K.
The VPS4 gene is involved in protein transport out of a yeast pre-vacuolar endosome-like compartment
Curr. Genet.
31
469-480
1997
Saccharomyces cerevisiae, Homo sapiens, Mus musculus, Schizosaccharomyces pombe
Manually annotated by BRENDA team
Babst, M.; Sato, T.K.; Banta, L.M.; Emr, S.D.
Endosomal transport function in yeast requires a novel AAA-type ATPase, Vps4p
EMBO J.
16
1820-1831
1997
Saccharomyces cerevisiae, Homo sapiens, Mus musculus, Schizosaccharomyces pombe
Manually annotated by BRENDA team
Patel, S.; Latterich, M.
The AAA team: related ATPases with diverse functions
Trends Cell Biol.
8
65-71
1998
Saccharomyces cerevisiae, Mus musculus, Drosophila melanogaster (P54351)
Manually annotated by BRENDA team
Yoshimori, T.; Yamagata, F.; Yamamoto, A.; Mizushima, N.; Kabeya, Y.; Nara, A.; Miwako, I.; Ohashi, M.; Ohsumi, M.; Ohsumi, Y.
The mouse SKD1, a homologue of yeast Vps4p, is required for normal endosomal trafficking and morphology in mammalian cells
Mol. Biol.
11
747-763
2000
Saccharomyces cerevisiae, Homo sapiens, Mus musculus, Rattus norvegicus
Manually annotated by BRENDA team
Fujita, H.; Umezuki, Y.; Imamura, K.; Ishikawa, D.; Uchimura, S.; Nara, A.; Yoshimori, T.; Hayashizaki, Y.; Kawai, J.; Ishidoh, K.; Tanaka, Y.; Himeno, M.
Mammalian class E Vps proteins, SBP1 and mVps2/CHMP2A, interact with and regulate the function of an AAA-ATPase SKD1/Vps4B
J. Cell Sci.
117
2997-3009
2004
Mus musculus
Manually annotated by BRENDA team
Dalal, S.; Rosser, M.F.N.; Cyr, D.M.; Hanson, P.I.
Distinct roles for the AAA ATPases NSF and p97 in the secretory pathway
Mol. Biol. Cell
15
637-648
2004
Cricetulus griseus, Mus musculus
Manually annotated by BRENDA team
Shiozawa Kumik, S.K.; Maita Nobu, M.N.; Tomii Kentar, T.K.; Seto Azus, S.A.; Goda Natsuk, G.N.; Akiyama Yutak, A.Y.; Shimizu Toshiyuk, S.T.; Shirakawa Masahir, S.M.; Hiroaki Hidekaz, H.H.
Crystallographic characterization of the N-terminal domain of PEX1
Acta Crystallogr. Sect. D
60
2098-2099
2004
Mus musculus
Manually annotated by BRENDA team
Ramalho-Santos, J.; Schatten, G.
Presence of N-ethyl maleimide sensitive factor (NSF) on the acrosome of mammalian sperm
Arch. Androl.
50
163-168
2004
Bos taurus, Mus musculus
Manually annotated by BRENDA team
Shiozawa Kumik, S.K.; Goda Natsuk, G.N.; Shimizu Toshiyuk, S.T.; Mizuguchi Kenj, M.K.; Kondo Naom, K.N.; Shimozawa Nobuyuk, S.N.; Shirakawa Masahir, S.M.; Hiroaki Hidekaz, H.H.
The common phospholipid-binding activity of the N-terminal domains of PEX1 and VCP/p97
FEBS J.
273
4959-4971
2006
Mus musculus
Manually annotated by BRENDA team
Morrell, C.N.; Matsushita, K.; Lowenstein, C.J.
A novel inhibitor of N-ethylmaleimide-sensitive factor decreases leukocyte trafficking and peritonitis
J. Pharmacol. Exp. Ther.
314
155-161
2005
Mus musculus
Manually annotated by BRENDA team
Steinberg, J.P.; Huganir, R.L.; Linden, D.J.
N-ethylmaleimide-sensitive factor is required for the synaptic incorporation and removal of AMPA receptors during cerebellar long-term depression
Proc. Natl. Acad. Sci. USA
101
18212-18216
2004
Mus musculus
Manually annotated by BRENDA team
Liu, Y.; Cheng, K.; Gong, K.; Fu, A.K.; Ip, N.Y.
Pctaire1 phosphorylates N-ethylmaleimide-sensitive fusion protein: implications in the regulation of its hexamerization and exocytosis
J. Biol. Chem.
281
9852-9858
2006
Mus musculus, Rattus norvegicus
Manually annotated by BRENDA team
Oh, E.; Heise, C.J.; English, J.M.; Cobb, M.H.; Thurmond, D.C.
WNK1 is a novel regulator of Munc18c-syntaxin 4 complex formation in soluble NSF attachment protein receptor (SNARE)-mediated vesicle exocytosis
J. Biol. Chem.
282
32613-32622
2007
Mus musculus
Manually annotated by BRENDA team
Inoue, M.; Kamikubo, H.; Kataoka, M.; Kato, R.; Yoshimori, T.; Wakatsuki, S.; Kawasaki, M.
Nucleotide-dependent conformational changes and assembly of the AAA ATPase SKD1/VPS4B
Traffic
9
2180-2189
2008
Mus musculus (P46467)
Manually annotated by BRENDA team
Xia, Y.; Liu, N.; Xie, X.; Bi, G.; Ba, H.; Li, L.; Zhang, J.; Deng, X.; Yao, Y.; Tang, Z.; Yin, B.; Wang, J.; Jiang, K.; Li, Z.; Choi, Y.; Gong, F.; Cheng, X.; OShea, J.J.; Chae, J.J.; Laurence, A.; Yang, X.P.
The macrophage-specific V-ATPase subunit ATP6V0D2 restricts inflammasome activation and bacterial infection by facilitating autophagosome-lysosome fusion
Autophagy
15
960-975
2019
Mus musculus
Manually annotated by BRENDA team