Information on EC 3.6.1.29 - bis(5'-adenosyl)-triphosphatase

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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria

EC NUMBER
COMMENTARY hide
3.6.1.29
-
RECOMMENDED NAME
GeneOntology No.
bis(5'-adenosyl)-triphosphatase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
P1,P3-bis(5'-adenosyl) triphosphate + H2O = ADP + AMP
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Purine metabolism
-
-
SYSTEMATIC NAME
IUBMB Comments
P1,P3-bis(5'-adenosyl)-triphosphate adenylohydrolase
-
CAS REGISTRY NUMBER
COMMENTARY hide
63951-94-0
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
shrimp
-
-
Manually annotated by BRENDA team
gene FHIT fragment; gene FHIT
SwissProt
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
-
a loss of FHIT expression associated with poor prognosis and beta-catenin expression
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2'-deoxyadenosine 5'-phosphoimidazolide + UDP
imidazole + dA(5')p3(5')U
show the reaction diagram
-
H96G mutant
-
-
?
5'-O-[amino(hydroxy)phosphoryl]adenosine + H2O
?
show the reaction diagram
5'-O-[fluoro(hydroxy)phosphoryl]adenosine + H2O
?
show the reaction diagram
5'-O-[hydroxy(sulfooxy)phosphoryl]adenosine + H2O
?
show the reaction diagram
7-benzylguanosine(5')triphospho(5')adenosine + H2O
7-benzylguanosine(5')monophosphate + 7-benzylguanosine(5')diphosphate
show the reaction diagram
-
-
-
-
?
7-ethylguanosine(5')triphospho(5')adenosine + H2O
7-ethylguanosine(5')monophosphate + 7-ethylguanosine(5')diphosphate
show the reaction diagram
-
-
-
-
?
7-methylguanosine(5')triphospho(5')7-methylguanosine + H2O
7-methylguanosine(5')monophosphate + 7-methylguanosine(5')diphosphate
show the reaction diagram
-
-
-
-
?
7-methylguanosine(5')triphospho(5')adenosine + H2O
7-methylguanosine(5')monophosphate + ADP + AMP + 7-methylguanosine(5')diphosphate
show the reaction diagram
7-methylguanosine(5')triphospho(5')cytosine + H2O
7-methylguanosine(5')monophosphate + CDP + CMP + 7-methylguanosine(5')diphosphate
show the reaction diagram
-
-
-
-
?
7-methylguanosine(5')triphospho(5')guanosine + H2O
7-methylguanosine(5')monophosphate + GDP + 7-methylguanosine(5')diphosphate + GMP
show the reaction diagram
7-methylguanosine(5')triphospho(5')uracil + H2O
7-methylguanosine(5')monophosphate + UDP + UMP + 7-methylguanosine(5')diphosphate
show the reaction diagram
-
-
-
-
?
adenosine 5'-phosphoimidazolide + ADP
imidazole + A(5')p3(5')A
show the reaction diagram
-
H96G mutant
-
-
?
adenosine 5'-phosphoimidazolide + ATP
imidazole + A(5')p4(5')A
show the reaction diagram
-
H96G mutant
-
-
?
adenosine 5'-phosphoimidazolide + CDP
imidazole + A(5')p3(5')C
show the reaction diagram
-
H96G mutant
-
-
?
adenosine 5'-phosphoimidazolide + GDP
imidazole + A(5')p3(5')G
show the reaction diagram
-
H96G mutant
-
-
?
adenosine 5'-phosphoimidazolide + TDP
imidazole + A(5')p3(5')T
show the reaction diagram
-
H96G mutant
-
-
?
adenosine 5'-phosphoimidazolide + UDP
imidazole + A(5')p3(5')U
show the reaction diagram
-
H96G mutant
-
-
?
adenosine 5'-phosphoimidazolide + UTP
imidazole + A(5')p4(5')U
show the reaction diagram
-
H96G mutant
-
-
?
adenosine 5'-phosphoramidate + H2O
AMP + NH3
show the reaction diagram
adenosine 5'-phosphosulfate + H2O
AMP + sulfate
show the reaction diagram
adenosine-5'-phospho-N-methylimidazolide + H2O
?
show the reaction diagram
-
-
-
-
?
adenosine-5'-phosphoimidazolide + H2O
?
show the reaction diagram
-
-
-
-
?
adenosyl-cytosinyl triphosphate + H2O
CDP + AMP
show the reaction diagram
adenosyl-guanosine triphosphate + H2O
GDP + AMP
show the reaction diagram
adenosyl-ribose triphosphate + H2O
AMP + ribose diphosphate
show the reaction diagram
-
-
-
-
?
adenylylfluoride + H2O
AMP + fluoride
show the reaction diagram
-
-
-
?
Ap3A + H2O
ADP + AMP
show the reaction diagram
-
-
-
-
?
Ap4C + H2O
AMP + CTP
show the reaction diagram
-
-
-
-
?
Ap4G + H2O
AMP + GTP + ATP + GMP
show the reaction diagram
-
-
-
-
?
Ap4U + H2O
AMP + UTP
show the reaction diagram
-
-
-
-
?
Ap6A + H2O
?
show the reaction diagram
-
-
-
-
?
ApCF2ppA
?
show the reaction diagram
-
-
-
-
?
ApCH2ppA
?
show the reaction diagram
-
-
-
-
?
ApCHFppA
?
show the reaction diagram
-
-
-
-
?
Appp-S-(4,4-difluoro-5,7-dimethyl-4-bora-3a,4a-diaza-s-indacine-3-yl) methylaminoacetyl + H2O
AMP + diphospho-S-(4,4-difluoro-5,7-dimethyl-4-bora-3a,4a-diaza-s-indacine-3-yl) methylaminoacetyl
show the reaction diagram
-
-
-
-
?
Appp-S-(7-diethylamino-4-methyl-3-(4-succinimidylphenyl)) coumarin + H2O
AMP + diphospho-S-(7-diethylamino-4-methyl-3-(4-succinimidylphenyl)) coumarin
show the reaction diagram
-
-
-
-
?
ATP + 2 H2O
AMP + 2 phosphate
show the reaction diagram
-
-
-
-
?
cytosine 5'-phosphoimidazolide + UDP
imidazole + C(5')p3(5')U
show the reaction diagram
-
H96G mutant
-
-
?
di(7-methylguanosine) 50,50 0 0-P1,P3-triphosphate + H2O
?
show the reaction diagram
diadenosine 5',5''-P1,P4-tetraphosphate + H2O
AMP + ATP
show the reaction diagram
diadenosine 5',5''-P1,P5-pentaphosphate + H2O
AMP + adenosine 5'-tetraphosphate
show the reaction diagram
Dixanthosine triphosphate + H2O
?
show the reaction diagram
-
-
-
-
?
gamma-fluoro-ATP + H2O
AMP + fluorodiphosphoric acid
show the reaction diagram
-
-
-
?
Gp3C + H2O
GMP + CDP
show the reaction diagram
-
-
-
-
?
Gp4A + H2O
?
show the reaction diagram
-
-
-
-
?
Gp4G + H2O
GMP + GTP
show the reaction diagram
-
-
-
-
?
Gp5A + H2O
?
show the reaction diagram
-
-
-
-
?
Gppp-S-(4,4-difluoro-5,7-dimethyl-4-bora-3a,4a-diaza-s-indacine-3-yl) methylaminoacetyl + H2O
GMP + diphospho-S-(4,4-difluoro-5,7-dimethyl-4-bora-3a,4a-diaza-s-indacine-3-yl) methylaminoacetyl
show the reaction diagram
-
-
-
-
?
GpppA + H2O
GMP + ADP + GDP + AMP
show the reaction diagram
inosine 5'-phosphoramidate + H2O
IMP + NH3
show the reaction diagram
m27,2'OGpppG + H2O
ppm27,2'OG + guanosine monophosphate
show the reaction diagram
m2N2,7GpppG + H2O
pm2N2,7G + guanosine diphosphate
show the reaction diagram
-
-
-
-
?
m3N2,N2,7GpppA + H2O
ppm3N2,N2,7G + adenosine monophosphate
show the reaction diagram
-
-
-
-
?
m3N2,N2,7GpppG + H2O
ppm3N2,N2,7G + guanosine monophosphate
show the reaction diagram
-
-
-
-
?
m7GpppA + H2O
N7-methyl-GMP + ADP + N7-methyl-GDP + AMP
show the reaction diagram
-
-
-
-
?
m7GpppG + H2O
pm7G + guanosine diphosphate
show the reaction diagram
-
-
-
-
?
m7Gpppm3N6,N6,2'OA + H2O
pm7G + ppm3N6,N6,2'OA
show the reaction diagram
-
-
-
-
?
m7Gpppm7G + H2O
N7-methyl-GMP + N7-methyl-GDP
show the reaction diagram
-
-
-
-
?
m7GpppmN6A + H2O
pm7G + ppmN6A
show the reaction diagram
-
-
-
-
?
NAD+ + H2O
?
show the reaction diagram
-
-
-
-
?
P1,P3-bis(5'-(7-methylguanosyl))triphosphate + H2O
?
show the reaction diagram
P1,P3-bis(5'-adenosyl) triphosphate + H2O
ADP + AMP
show the reaction diagram
P1,P3-bis(5'-adenosyl)triphosphate + H2O
ADP + AMP
show the reaction diagram
P1,P3-bis(5'-adenosyl)triphosphate + H2O
AMP + ADP
show the reaction diagram
-
major substrate
-
-
?
P1,P4-bis(5'-adenosyl)tetraphosphate + H2O
AMP + ATP
show the reaction diagram
-
major substrate
-
-
?
P1,P4-bis(5-adenosyl)tetraphosphate + H2O
ADP + AMP
show the reaction diagram
P1-5'-O-adenosine-P3-5'-O-adenosine triphosphate + H2O
AMP + ADP
show the reaction diagram
-
-
-
-
r
P1-bis(5'-adenosyl) monophosphate + H2O
?
show the reaction diagram
P1-P3-bis(5'-adenosyl) triphosphate + H2O
ADP + AMP
show the reaction diagram
P1-P3-bis(5'-guanosyl) triphosphate + H2O
GDP + GMP
show the reaction diagram
P1-P3-bis(5'-uracyl) triphosphate + H2O
UDP + UMP
show the reaction diagram
P1-P5-bis(5'-adenosyl) pentaphosphate + H2O
adenosine monophosphate + adenosine tetraphosphate
show the reaction diagram
-
-
-
-
?
thymidine 5'-monophosphate p-nitrophenylester + H2O
?
show the reaction diagram
-
-
-
-
?
thymidine 5'-phosphoimidazolide + UDP
imidazole + T(5')p3(5')U
show the reaction diagram
-
H96G mutant
-
-
?
Up3G + H2O
GMP + UDP
show the reaction diagram
-
-
-
-
?
UTP + H2O
UMP + phosphate
show the reaction diagram
-
-
-
-
?
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
adenosine 5'-phosphoramidate + H2O
AMP + NH3
show the reaction diagram
adenosine 5'-phosphosulfate + H2O
AMP + sulfate
show the reaction diagram
adenylylfluoride + H2O
AMP + fluoride
show the reaction diagram
Q9LVM4
-
-
-
?
gamma-fluoro-ATP + H2O
AMP + fluorodiphosphoric acid
show the reaction diagram
Q9LVM4
-
-
-
?
P1,P3-bis(5'-adenosyl) triphosphate + H2O
ADP + AMP
show the reaction diagram
P1,P3-bis(5'-adenosyl)triphosphate + H2O
ADP + AMP
show the reaction diagram
P1-5'-O-adenosine-P3-5'-O-adenosine triphosphate + H2O
AMP + ADP
show the reaction diagram
-
-
-
-
r
P1-bis(5'-adenosyl) monophosphate + H2O
?
show the reaction diagram
Q9LVM4
requirement for Mg2+
-
-
?
P1-P3-bis(5'-adenosyl) triphosphate + H2O
ADP + AMP
show the reaction diagram
additional information
?
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Zn2+
-
-
additional information
-
no requirement for Mg2+
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
adenosine 5'-O-(gamma-fluoromonophosphate)
-
adenosine 5'-O-(gamma-fluorotriphosphate)
-
adenosine 5'-phosphosulfate
diethyl dicarbonate
-
inactivation
O,O',O''-tris[[(2R,3S,4R,5R)-5-(6-amino-9H-purin-9-yl)-3,4-dihydroxytetrahydrofuran-2-yl]methyl] O,O',O''-propane-1,2,3-triyl tris[hydrogen (phosphorothioate)]
-
-
O,O'-bis[[(2R,3S,4R,5R)-5-(6-amino-9H-purin-9-yl)-3,4-dihydroxytetrahydrofuran-2-yl]methyl] O,O'-(2-hydroxypropane-1,3-diyl) bis[hydrogen (phosphorothioate)]
-
-
O-[1,11-bis[(2R,3S,4R,5R)-5-(6-amino-9H-purin-9-yl)-3,4-dihydroxytetrahydrofuran-2-yl]-3,9-dioxido-3,9-disulfanyl-2,4,8,10-tetraoxa-3l5,9l5-diphosphaundecan-6-yl] dihydrogen phosphorothioate
-
-
O-[3-[[[[(2R,3S,4R,5R)-5-(6-amino-9H-purin-9-yl)-3,4-dihydroxytetrahydrofuran-2-yl]methoxy](sulfanyl)phosphoryl]oxy]-2,2-bis([[[[(2R,3S,4R,5R)-5-(6-amino-9H-purin-9-yl)-3,4-dihydroxytetrahydrofuran-2-yl]methoxy](sulfanyl)phosphoryl]oxy]methyl)propyl] dihydrogen phosphorothioate
-
-
O-[3-[[[[(2R,3S,4R,5R)-5-(6-amino-9H-purin-9-yl)-3,4-dihydroxytetrahydrofuran-2-yl]methoxy](sulfanyl)phosphoryl]oxy]-2,2-bis([[[[(2R,3S,4R,5R)-5-(6-amino-9H-purin-9-yl)-3,4-dihydroxytetrahydrofuran-2-yl]methoxy](sulfanyl)phosphoryl]oxy]methyl)propyl] O-[[(2R,3S,4R,5R)-5-(6-amino-9H-purin-9-yl)-3,4-dihydroxytetrahydrofuran-2-yl]methyl] hydrogen phosphorothioate
-
-
P1,P3-bis(5'-adenosyl) triphosphate
spermine
-
-
suramin
additional information
-
poor inhibition by fluoroadenylates, ATP, and ADP
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.003 - 0.015
5'-O-[amino(hydroxy)phosphoryl]adenosine
0.0053 - 0.18
adenosine-5'-phospho-N-methylimidazolide
0.0062 - 0.026
adenosine-5'-phosphoimidazolide
0.0006 - 0.0046
Ap4A
0.013
Ap5A
-
-
0.212
ApCF2ppA
-
-
0.117
ApCH2ppA
-
-
0.09
ApCHFppA
-
-
0.00066 - 0.0016
P1,P3-bis(5'-adenosyl)triphosphate
0.0029 - 0.026
P1-5'-O-adenosine-P3-5'-O-adenosine triphosphate
0.001 - 0.015
P1-P3-bis(5'-adenosyl) triphosphate
0.002 - 0.04
P1-P3-bis(5'-guanosyl) triphosphate
0.025
P1-P3-bis(5'-uracyl) triphosphate
-
-
20
UDP
-
pH 7, synthesis of Ap3U by H96G mutant
additional information
additional information
-
steady state kinetics of the phosphorylated enzyme form
-
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1.26
5'-O-[amino(hydroxy)phosphoryl]adenosine
0.62 - 4.2
adenosine-5'-phospho-N-methylimidazolide
0.91 - 4.4
adenosine-5'-phosphoimidazolide
0.02 - 6
P1,P3-bis(5'-adenosyl)triphosphate
0.0014 - 3.5
P1-5'-O-adenosine-P3-5'-O-adenosine triphosphate
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.018 - 0.235
adenosine 5'-O-(gamma-fluoromonophosphate)
0.0071 - 0.0136
adenosine 5'-phosphosulfate
0.14
ADP
pH 6.8, 30C
0.11 - 0.113
AMP
0.145
ATP
pH 6.8, 30C
0.0021 - 0.018
P1,P3-bis(5'-adenosyl) triphosphate
0.000022
suramin
-
pH 7.0, 37C
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.002
suramin
Homo sapiens
-
strong, competitive inhibition, IC50: 0.002 mM
0.02
Zn2+
Homo sapiens
-
IC50: 0.02 mM
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.0075
-
-
0.02
-
-
0.4
-
skeletal muscle, 27000 x g supernatant
1.34
-
purified native enzyme from leukocytes
1.8
-
brain, 27000 x g supernatant
4.8
-
liver, 27000 x g precipitate
13
-
kidney, 27000 x g precipitate
290
-
Ap3Aase-alpha
300
-
Ap3Aase-beta
additional information
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.8
-
optimal pH for hydrolysis of adenosine-5'-phosphoimidazolide
6.6
-
optimal pH for hydrolysis of adenosine-5'-phospho-N-methylimidazolide
7 - 7.2
-
-
7.3
-
optimal pH for hydrolysis of P1-5'-O-adenosine-P3-5'-O-adenosine triphosphate
8.2 - 8.5
-
-
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4.6 - 10
-
-
6.6 - 9.5
-
-
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
37
-
assay at
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
25 - 45
-
-
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
a human SV40-immortalized normal bronchial epithelial cell line
Manually annotated by BRENDA team
-
colonic cancer cell line
Manually annotated by BRENDA team
-
colonic cancer cell line
Manually annotated by BRENDA team
-
colonic cancer cell line
Manually annotated by BRENDA team
-
FHIT expression level and distribution in neoplastic, hyperplastic, and healthy endometrium, overview
Manually annotated by BRENDA team
-
of secretory endometrium
Manually annotated by BRENDA team
-
of secretory endometrium
Manually annotated by BRENDA team
-
peripheral
Manually annotated by BRENDA team
-
colonic cancer cell line
Manually annotated by BRENDA team
additional information
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
29800
-
gel filtration
34000
-
gel filtration
55000
-
gel filtration
115000
-
gel filtration
230000
-
gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
homodimer
monomer
polymer
-
-
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
glycoprotein
-
mannoside
phosphoprotein
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20C, 50 mM HEPES-NaOH buffer, pH 7.0, 100 mM NaCl, 25% glycerol, at least 18 months
-
4C, 50 mM HEPES-NaOH buffer, pH 7.0, 100 mM NaCl, 3 weeks
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
co-expression of Fhit with the elk tyrosine kinase in Escherichia coli strain TBK1 by ion exchange chromatography, gel filtration, and another step of ion exchange chromatography
-
native enzyme from blood platelets and leukocytes, 19000fold, by anion exchange chromatography and N6-5'-AMP affinity chromatography to homogeneity
-
native enzyme from brain cytosolic fraction by ammonium sulfate fractionation, anion exchange chromatography, gel filtration, and N6-5'-AMP affinity chromatography to homogeneity
-
Ni-NTA agarose column chromatography
-
partially
-
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
co-expression of Fhit with the elk tyrosine kinase in Escherichia coli strain TBK1, expression of nonphosphorylized enzyme in Escherichia coli strain SG100
-
constitutive expression of FHIT in Calu-1 cells, endogenously lacking FHIT protein, FHIT re-expression does not affect cell survival, but a Calu-1 cell clone in which the expression of FHIT is under the control of a CMV promoter, is more resistant to TRAIL-induced, i.e. tumor necrosis factor-related apoptosis inducing ligand-induced, apoptosis than the mock transfected Calu-1 clones
-
expressed in Escherichia coli BL21(DE3) cells
-
expression in COS1 cells; expression in Escherichia coli
-
expression in Escherichia coli
-
FHIT expression analysis of wild-type and mutant enzyme in colon cancer cell lines, overview
-
gene At5g58240, DNA and amino acid sequence determination and analysis
gene fhit maps to chromosome 3q14.2, expression analysis in human tissues, overview
-
gene FHIT, DNA sequence determination and analysis, comparative mapping study, the gene locates to BTA22q24prox
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
FHIT is absent or reduced in many types of human tumors including lung, esophagus, stomach, kidney and cervical carcinomas
-
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
FHIT-specific small interference RNA transfection of SV40-immortalized normal bronchial BEAS cells that show levels of FHIT protein comparable to those of normal bronchial cells, results in a significant increase of TRAIL-induced apoptosis