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(+)-FTCMP + H2O
?
-
-
-
-
?
2',2'-difluoro-dCMP + H2O
2',2'-difluoro-dUMP + NH3
-
-
-
-
?
2',3'-dideoxy-5-fluoro-3'-thiacytidine + H2O
?
-
good substrate
-
-
?
5-aza-dCMP + H2O
5-aza-dUMP + NH3
-
-
-
-
?
5-bromo-dCMP + H2O
5-bromo-dUMP + NH3
5-chloro-dCMP + H2O
5-chloro-dUMP + NH3
5-fluoro-dCMP + H2O
5-fluoro-dUMP + NH3
5-Hg-dCMP + H2O
5-Hg-dUMP + NH3
5-hydroxymethyl-dCMP + H2O
5-hydroxymethyl-dUMP + NH3
5-iodo-dCMP + H2O
5-iodo-dUMP + NH3
5-methyl-dCMP + H2O
5-methyl-dUMP + NH3
5-methyl-dCMP + H2O
dTMP + NH3
ara-CMP + H2O
ara-UMP + NH3
beta-D-2',2'-difluorodeoxycytidine + H2O
beta-D-2',2'-difluorodeoxyuridine + NH3
-
-
-
-
?
dCMP-Hg-S-CH2-CH2-OH + H2O
dUMP-Hg-S-CH2-CH2-OH + NH3
-
better substrate of T form: in the presence of dTTP
-
-
?
dCTP + H2O
dUTP + NH3
-
-
-
?
deoxycytidine + H2O
deoxyuridine + NH3
PSI-6130-MP + H2O
?
-
weak substrate
-
-
?
additional information
?
-
5-bromo-dCMP + H2O
5-bromo-dUMP + NH3
-
-
-
-
?
5-bromo-dCMP + H2O
5-bromo-dUMP + NH3
-
-
-
-
?
5-bromo-dCMP + H2O
5-bromo-dUMP + NH3
-
very good substrate
-
-
?
5-bromo-dCMP + H2O
5-bromo-dUMP + NH3
-
-
-
-
?
5-bromo-dCMP + H2O
5-bromo-dUMP + NH3
-
very poor substrate
-
-
?
5-chloro-dCMP + H2O
5-chloro-dUMP + NH3
-
-
-
-
?
5-chloro-dCMP + H2O
5-chloro-dUMP + NH3
-
very poor substrate
-
-
?
5-fluoro-dCMP + H2O
5-fluoro-dUMP + NH3
-
-
-
-
?
5-fluoro-dCMP + H2O
5-fluoro-dUMP + NH3
-
-
-
-
?
5-fluoro-dCMP + H2O
5-fluoro-dUMP + NH3
-
very good substrate
-
-
?
5-fluoro-dCMP + H2O
5-fluoro-dUMP + NH3
-
very good substrate
-
-
?
5-fluoro-dCMP + H2O
5-fluoro-dUMP + NH3
-
-
-
-
?
5-fluoro-dCMP + H2O
5-fluoro-dUMP + NH3
-
very poor substrate
-
-
?
5-Hg-dCMP + H2O
5-Hg-dUMP + NH3
-
deamination activated by dTTP and inhibited by dCTP
-
-
?
5-Hg-dCMP + H2O
5-Hg-dUMP + NH3
-
5-mercury derivative of dCMP only in the presence of an excess of mercaptoethanol
-
-
?
5-Hg-dCMP + H2O
5-Hg-dUMP + NH3
-
very poor substrate in the absence of mercaptoethanol
-
-
?
5-Hg-dCMP + H2O
5-Hg-dUMP + NH3
-
deamination activated by dTTP and inhibited by dCTP
-
-
?
5-Hg-dCMP + H2O
5-Hg-dUMP + NH3
-
5-mercury derivative of dCMP only in the presence of an excess of mercaptoethanol
-
-
?
5-hydroxymethyl-dCMP + H2O
5-hydroxymethyl-dUMP + NH3
-
-
-
-
?
5-hydroxymethyl-dCMP + H2O
5-hydroxymethyl-dUMP + NH3
-
-
-
-
?
5-hydroxymethyl-dCMP + H2O
5-hydroxymethyl-dUMP + NH3
-
very poor substrate
-
-
?
5-hydroxymethyl-dCMP + H2O
5-hydroxymethyl-dUMP + NH3
-
-
-
-
?
5-hydroxymethyl-dCMP + H2O
5-hydroxymethyl-dUMP + NH3
-
HeLa cells, good substrate
-
-
?
5-hydroxymethyl-dCMP + H2O
5-hydroxymethyl-dUMP + NH3
Tequatrovirus T4
-
-
-
-
?
5-hydroxymethyl-dCMP + H2O
5-hydroxymethyl-dUMP + NH3
Tequatrovirus T4
-
very poor substrate
-
-
?
5-iodo-dCMP + H2O
5-iodo-dUMP + NH3
-
-
-
-
?
5-iodo-dCMP + H2O
5-iodo-dUMP + NH3
-
very poor substrate
-
-
?
5-iodo-dCMP + H2O
5-iodo-dUMP + NH3
-
-
-
-
?
5-iodo-dCMP + H2O
5-iodo-dUMP + NH3
-
very poor substrate
-
-
?
5-methyl-dCMP + H2O
5-methyl-dUMP + NH3
-
-
-
-
?
5-methyl-dCMP + H2O
5-methyl-dUMP + NH3
-
-
-
-
?
5-methyl-dCMP + H2O
5-methyl-dUMP + NH3
-
-
-
-
?
5-methyl-dCMP + H2O
5-methyl-dUMP + NH3
-
very good substrate
-
-
?
5-methyl-dCMP + H2O
5-methyl-dUMP + NH3
-
very good substrate
-
-
?
5-methyl-dCMP + H2O
5-methyl-dUMP + NH3
-
-
-
-
?
5-methyl-dCMP + H2O
5-methyl-dUMP + NH3
-
HeLa cells, good substrate
-
-
?
5-methyl-dCMP + H2O
5-methyl-dUMP + NH3
-
-
-
-
?
5-methyl-dCMP + H2O
5-methyl-dUMP + NH3
-
5-methyl-dCMP: very poor substrate
-
-
?
5-methyl-dCMP + H2O
dTMP + NH3
-
-
-
-
?
5-methyl-dCMP + H2O
dTMP + NH3
-
deamination pathway, overview
-
-
?
ara-CMP + H2O
ara-UMP + NH3
-
deaminated only in the presence of dCTP, strongly activated by dCTP
-
-
?
ara-CMP + H2O
ara-UMP + NH3
-
arabinosyl-CMP
-
-
?
ara-CMP + H2O
ara-UMP + NH3
-
at a very low rate
-
-
?
ara-CMP + H2O
ara-UMP + NH3
-
effective substrate in presence of dCTP
-
-
?
ara-CMP + H2O
ara-UMP + NH3
-
arabinosyl-CMP
-
-
?
ara-CMP + H2O
ara-UMP + NH3
-
1-beta-D-arabinofuranosyl-CMP
-
-
?
ara-CMP + H2O
ara-UMP + NH3
-
effective substrate in presence of dCTP
-
-
?
ara-CMP + H2O
ara-UMP + NH3
-
fair substrate
-
-
?
CMP + H2O
UMP + NH3
-
slight activity, about 5% compared with dCMP
-
-
?
CMP + H2O
UMP + NH3
-
slight activity, about 5% compared with dCMP
-
-
?
CMP + H2O
UMP + NH3
-
poor substrate
-
-
?
CMP + H2O
UMP + NH3
-
deaminated only in the presence of dCTP, strongly activated by dCTP
-
-
?
CMP + H2O
UMP + NH3
-
at a low rare, strongly activated by dCTP
-
-
?
CMP + H2O
UMP + NH3
-
-
-
-
?
CMP + H2O
UMP + NH3
-
poor substrate
-
-
?
CMP + H2O
UMP + NH3
-
HeLa cells, at 1 mM, in presence and absence of dCTP and Mg2+: not a substrate
-
-
?
CMP + H2O
UMP + NH3
Tequatrovirus T4
-
-
-
-
?
dCMP + H2O
dUMP + NH3
-
-
-
?
dCMP + H2O
dUMP + NH3
-
-
-
?
dCMP + H2O
dUMP + NH3
-
providing an adequate supply of dUMP for formation of dTMP, via dTMP synthase a crucial intermediate for synthesis of DNA
-
-
?
dCMP + H2O
dUMP + NH3
-
importance in the generation of dUMP, precursor for synthesis of thymidine nucleotides
-
?
dCMP + H2O
dUMP + NH3
-
importance in the generation of dUMP, precursor for synthesis of thymidine nucleotides
-
-
?
dCMP + H2O
dUMP + NH3
-
-
-
?
dCMP + H2O
dUMP + NH3
-
importance in the generation of dUMP, precursor for synthesis of thymidine nucleotides
-
?
dCMP + H2O
dUMP + NH3
-
-
-
?
dCMP + H2O
dUMP + NH3
bacteriophage SP8
-
-
-
?
dCMP + H2O
dUMP + NH3
Betapolyomavirus macacae
-
-
-
?
dCMP + H2O
dUMP + NH3
Betapolyomavirus macacae
-
providing an adequate supply of dUMP for formation of dTMP, via dTMP synthase a crucial intermediate for synthesis of DNA
-
-
?
dCMP + H2O
dUMP + NH3
Betapolyomavirus macacae
-
importance in the generation of dUMP, precursor for synthesis of thymidine nucleotides
-
-
?
dCMP + H2O
dUMP + NH3
-
-
-
?
dCMP + H2O
dUMP + NH3
-
the dCMP concentration at half-maximum velocity is lower by enzyme from Herpes simplex virus infected BHK-21/C13 cells from baby-hamster than by enzyme from non-infected cells
-
?
dCMP + H2O
dUMP + NH3
-
enzyme involved in main pathway leading to synthesis of dTTP, role in provision of precursors for DNA synthesis
-
-
?
dCMP + H2O
dUMP + NH3
-
enzyme involved in main pathway leading to synthesis of dTTP, role in provision of precursors for DNA synthesis
-
?
dCMP + H2O
dUMP + NH3
-
-
-
?
dCMP + H2O
dUMP + NH3
-
-
-
?
dCMP + H2O
dUMP + NH3
-
providing an adequate supply of dUMP for formation of dTMP, via dTMP synthase a crucial intermediate for synthesis of DNA
-
-
?
dCMP + H2O
dUMP + NH3
-
importance in the generation of dUMP, precursor for synthesis of thymidine nucleotides
-
-
?
dCMP + H2O
dUMP + NH3
-
-
-
?
dCMP + H2O
dUMP + NH3
-
-
-
?
dCMP + H2O
dUMP + NH3
-
providing an adequate supply of dUMP for formation of dTMP, via dTMP synthase a crucial intermediate for synthesis of DNA
-
-
?
dCMP + H2O
dUMP + NH3
-
importance in the generation of dUMP, precursor for synthesis of thymidine nucleotides
-
-
?
dCMP + H2O
dUMP + NH3
-
-
-
?
dCMP + H2O
dUMP + NH3
-
-
-
?
dCMP + H2O
dUMP + NH3
-
-
-
?
dCMP + H2O
dUMP + NH3
-
-
-
?
dCMP + H2O
dUMP + NH3
-
-
-
?
dCMP + H2O
dUMP + NH3
-
-
-
?
dCMP + H2O
dUMP + NH3
-
not a substrate of the T form, dTTP-induced form of the enzyme
-
?
dCMP + H2O
dUMP + NH3
-
catalyzed reaction is at a branching point of the pathway leading to dCTP and dTTP, immediate pyrimidine deoxynucleotide precursors in DNA biosynthesis
-
?
dCMP + H2O
dUMP + NH3
-
providing an adequate supply of dUMP for formation of dTMP, via dTMP synthase a crucial intermediate for synthesis of DNA
-
-
?
dCMP + H2O
dUMP + NH3
-
importance in the generation of dUMP, precursor for synthesis of thymidine nucleotides
-
-
?
dCMP + H2O
dUMP + NH3
-
-
-
?
dCMP + H2O
dUMP + NH3
-
-
-
?
dCMP + H2O
dUMP + NH3
-
-
-
?
dCMP + H2O
dUMP + NH3
-
-
-
?
dCMP + H2O
dUMP + NH3
-
-
-
?
dCMP + H2O
dUMP + NH3
-
true substrate probably dCMP-Mg complex
-
?
dCMP + H2O
dUMP + NH3
-
major function to provide supplementary route for dTMP synthesis, located at a branch point in pyrimidine metabolic pathway
-
-
?
dCMP + H2O
dUMP + NH3
-
enzyme involved in formation of dTMP
-
-
?
dCMP + H2O
dUMP + NH3
-
providing an adequate supply of dUMP for formation of dTMP, via dTMP synthase a crucial intermediate for synthesis of DNA
-
-
?
dCMP + H2O
dUMP + NH3
-
enzyme within pyrimidine deoxynucleotide pathway
-
-
?
dCMP + H2O
dUMP + NH3
-
enzyme plays an important if not rate controlling step in cell division
-
-
?
dCMP + H2O
dUMP + NH3
-
regulating dTMP for DNA synthesis
-
-
?
dCMP + H2O
dUMP + NH3
-
pathway for synthesis of dUMP, nucleotide required for synthesis of dTMP
-
-
?
dCMP + H2O
dUMP + NH3
-
importance in the generation of dUMP, precursor for synthesis of thymidine nucleotides
-
-
?
dCMP + H2O
dUMP + NH3
-
-
-
?
dCMP + H2O
dUMP + NH3
-
-
-
?
dCMP + H2O
dUMP + NH3
-
-
-
?
dCMP + H2O
dUMP + NH3
-
-
-
?
dCMP + H2O
dUMP + NH3
-
major function to provide supplementary route for dTMP synthesis, located at a branch point in pyrimidine metabolic pathway
-
-
?
dCMP + H2O
dUMP + NH3
-
enzyme involved in formation of dTMP
-
-
?
dCMP + H2O
dUMP + NH3
-
providing an adequate supply of dUMP for formation of dTMP, via dTMP synthase a crucial intermediate for synthesis of DNA
-
-
?
dCMP + H2O
dUMP + NH3
-
enzyme within pyrimidine deoxynucleotide pathway
-
-
?
dCMP + H2O
dUMP + NH3
-
enzyme plays an important if not rate controlling step in cell division
-
-
?
dCMP + H2O
dUMP + NH3
-
regulating dTMP for DNA synthesis
-
-
?
dCMP + H2O
dUMP + NH3
-
importance in the generation of dUMP, precursor for synthesis of thymidine nucleotides
-
-
?
dCMP + H2O
dUMP + NH3
Herpes simplex virus
-
-
-
?
dCMP + H2O
dUMP + NH3
Herpes simplex virus
-
the dCMP concentration at half-maximum velocity is lower by enzyme from Herpes simplex virus infected BHK-21/C13 cells from baby-hamster than by enzyme from non-infected cells
-
?
dCMP + H2O
dUMP + NH3
Herpes simplex virus
-
enzyme involved in main pathway leading to synthesis of dTTP, role in provision of precursors for DNA synthesis
-
-
?
dCMP + H2O
dUMP + NH3
Herpes simplex virus
-
providing an adequate supply of dUMP for formation of dTMP, via dTMP synthase a crucial intermediate for synthesis of DNA
-
-
?
dCMP + H2O
dUMP + NH3
Herpes simplex virus
-
importance in the generation of dUMP, precursor for synthesis of thymidine nucleotides
-
-
?
dCMP + H2O
dUMP + NH3
-
-
-
?
dCMP + H2O
dUMP + NH3
-
-
-
?
dCMP + H2O
dUMP + NH3
-
-
-
?
dCMP + H2O
dUMP + NH3
-
-
-
?
dCMP + H2O
dUMP + NH3
-
-
-
-
?
dCMP + H2O
dUMP + NH3
-
most dramatic allosteric response at substrate levels 0.1 mM dCMP or less
-
?
dCMP + H2O
dUMP + NH3
-
high degree of specificity of dCMP
-
ir
dCMP + H2O
dUMP + NH3
-
enzyme contributes formation of DNA by providing dUMP as substrate for thymidylate synthase. Potential role in cell division
-
?
dCMP + H2O
dUMP + NH3
-
providing an adequate supply of dUMP for formation of dTMP, via dTMP synthase a crucial intermediate for synthesis of DNA
-
-
?
dCMP + H2O
dUMP + NH3
-
key enzyme in pyrimidine deoxyribonucleotide metabolism, catalyzing the rate-limiting step, plays an important role in providing a balanced supply of dCTP and dTTP for DNA synthesis
-
?
dCMP + H2O
dUMP + NH3
-
important role in regulation of intracellular TTP levels
-
-
ir
dCMP + H2O
dUMP + NH3
-
importance in the generation of dUMP, precursor for synthesis of thymidine nucleotides
-
-
?
dCMP + H2O
dUMP + NH3
-
enzyme is major source of mammalian cell dUMP pool and regulates the synthesis of TMP
-
-
ir
dCMP + H2O
dUMP + NH3
-
the enzyme is important in the de novo synthesis of thymidine nucleotides, and may also be involved in the metabolism of anticancer agents
-
-
?
dCMP + H2O
dUMP + NH3
-
the enzyme plays a key role in the thymidylate synthase, pathway overview
-
-
?
dCMP + H2O
dUMP + NH3
-
-
-
?
dCMP + H2O
dUMP + NH3
-
-
-
?
dCMP + H2O
dUMP + NH3
-
one of the regulatory enzymes of DNA biosynthesis
-
?
dCMP + H2O
dUMP + NH3
-
-
-
?
dCMP + H2O
dUMP + NH3
-
enzyme of pyrimidine deoxyribonucleotide metabolism, important for the synthesis of thymidine nucleotides from thymine. Enzyme could provide the major means of dUMP formation
-
?
dCMP + H2O
dUMP + NH3
-
-
-
-
?
dCMP + H2O
dUMP + NH3
-
-
-
?
dCMP + H2O
dUMP + NH3
the enzyme is crucial to the production of dTTP needed for DNA replication and damage repair. It mediates dNTP pool balance required for normal chloroplast development and vegetative growth in plants
-
-
?
dCMP + H2O
dUMP + NH3
-
dUMP is a key intermediate in the synthesis of dTTP and subsequently of DNA synthesis
-
?
dCMP + H2O
dUMP + NH3
-
-
-
?
dCMP + H2O
dUMP + NH3
-
providing an adequate supply of dUMP for formation of dTMP, via dTMP synthase a crucial intermediate for synthesis of DNA
-
-
?
dCMP + H2O
dUMP + NH3
-
importance in the generation of dUMP, precursor for synthesis of thymidine nucleotides
-
-
?
dCMP + H2O
dUMP + NH3
-
-
-
?
dCMP + H2O
dUMP + NH3
-
providing an adequate supply of dUMP for formation of dTMP, via dTMP synthase a crucial intermediate for synthesis of DNA
-
-
?
dCMP + H2O
dUMP + NH3
-
importance in the generation of dUMP, precursor for synthesis of thymidine nucleotides
-
-
?
dCMP + H2O
dUMP + NH3
-
-
-
?
dCMP + H2O
dUMP + NH3
-
-
-
-
?
dCMP + H2O
dUMP + NH3
-
highly specific for dCMP
-
?
dCMP + H2O
dUMP + NH3
-
providing an adequate supply of dUMP for formation of dTMP, via dTMP synthase a crucial intermediate for synthesis of DNA
-
-
?
dCMP + H2O
dUMP + NH3
-
importance in the generation of dUMP, precursor for synthesis of thymidine nucleotides
-
-
?
dCMP + H2O
dUMP + NH3
-
deoxycytidine nucleotides are shunted into dTMP biosynthetic pathway via reaction catalyzed by dCMP deaminase
-
?
dCMP + H2O
dUMP + NH3
-
-
-
-
?
dCMP + H2O
dUMP + NH3
-
-
-
?
dCMP + H2O
dUMP + NH3
-
the regulation signal is transmitted by Arg4 from the allosteric site to the active site via modifications in the interactions at the interface where the substrate binding pocket is involved and the relocations of Arg26, His65, Tyr120, and Arg121 to envelope the active site in order to stabilize substrate binding in the complex, allosteric mechanism for enzyme regulation, regulator binding structure, overview
-
-
?
dCMP + H2O
dUMP + NH3
-
a conserved motif, G43YNG46, is involved in the binding of dCTP, N-terminal Arg4, a key residue located between two monomers, binds strongly to the gamma phosphate group of dCTP, active site structure and substrate binding, overview
-
-
?
dCMP + H2O
dUMP + NH3
Tequatrovirus T4
-
-
-
?
dCMP + H2O
dUMP + NH3
Tequatrovirus T4
-
-
-
?
dCMP + H2O
dUMP + NH3
Tequatrovirus T4
-
-
-
?
dCMP + H2O
dUMP + NH3
Tequatrovirus T4
-
-
-
?
dCMP + H2O
dUMP + NH3
Tequatrovirus T4
-
-
-
-
?
dCMP + H2O
dUMP + NH3
Tequatrovirus T4
-
major function to provide supplementary route for dTMP synthesis, located at a branch point in pyrimidine metabolic pathway
-
-
?
dCMP + H2O
dUMP + NH3
Tequatrovirus T4
-
providing an adequate supply of dUMP for formation of dTMP, via dTMP synthase a crucial intermediate for synthesis of DNA
-
-
?
dCMP + H2O
dUMP + NH3
Tequatrovirus T4
-
critical branchpoint in pyrimidine deoxynucleotide metabolism
-
-
?
dCMP + H2O
dUMP + NH3
Tequatrovirus T4
-
importance in the generation of dUMP, precursor for synthesis of thymidine nucleotides
-
-
?
dCMP + H2O
dUMP + NH3
Tequatrovirus T4
-
the enzyme is the major supplier of substrate for the thymidylate synthase important in DNA synthesis
-
-
?
deoxycytidine + H2O
deoxyuridine + NH3
-
-
-
-
?
deoxycytidine + H2O
deoxyuridine + NH3
-
the enzyme is a key enzyme in the inactivation of deoxycytidine and several chemotherapeutically important nucleoside analogues
-
-
?
additional information
?
-
-
substrate specificity
-
-
?
additional information
?
-
-
substrate specificity
-
-
?
additional information
?
-
-
no activity with dCDP, dCTP, CDP, CTP
-
-
?
additional information
?
-
-
substrate specificity
-
-
?
additional information
?
-
-
no activity with dCDP, dCTP, CDP, CTP
-
-
?
additional information
?
-
no deamination activity with dCTP or CMP
-
-
?
additional information
?
-
-
substrate specificity
-
-
?
additional information
?
-
-
substrate specificity
-
-
?
additional information
?
-
-
substrate specificity
-
-
?
additional information
?
-
-
substrate specificity
-
-
?
additional information
?
-
-
catalyzes deamination of halogenated derivatives of dCMP, but appears to be restricted in its ability to deaminate substrates with a large group in the 5-position
-
-
?
additional information
?
-
-
substrate specificity
-
-
?
additional information
?
-
-
-
-
-
?
additional information
?
-
-
substrate specificity
-
-
?
additional information
?
-
-
substrate specificity
-
-
?
additional information
?
-
-
substrate specificity
-
-
?
additional information
?
-
-
HeLa cells, no activity with CDP at 1 mM, in presence and absence of dCTP and Mg2+
-
-
?
additional information
?
-
-
enzyme is involved in elimination of some dCyd analogues, such as 2',2'-difluorodeoxycytidine, fluoro-dCyd, and ara-C, from tissues or cultured cells
-
-
?
additional information
?
-
-
the enzyme also performs 5-methylcytidyl-DNA deaminase activity but to a lesser extent, deamination pathway, overview
-
-
?
additional information
?
-
-
the human cytomegalovirus requires the cellular host dCMP deaminase for replication in quiescent cells, overview
-
-
?
additional information
?
-
-
substrate specificity with deoxycytidine analogue monophosphates, overview
-
-
?
additional information
?
-
-
the enzyme also performs 5-methylcytidyl-DNA deaminase activity but to a lesser extent, substrate specificity, overview
-
-
?
additional information
?
-
-
cytidine monophosphate is not a good substrate for dCMPD. Monophosphates of deoxycytidine analogs nucleosides in the L-conformation (e.g. apricitabine, lamivudine, and emtricitabine) and dideoxycytidine do not act as substrates of dCMPD
-
-
?
additional information
?
-
the bifunctional dCMP-dCTP deaminase shows dCMP deaminase and dCTP deaminase activities, dCTP serves as a positive heterotropic effector for the dCMP deaminase activity and a positive homotropic effector for the dCTP deaminase activity, and the enzymatic efficiency of the dCMP deaminase activity is about four times higher than that of the dCTP deaminase activity, the same active site is involved in both dCMP and dCTP deaminations
-
-
?
additional information
?
-
-
the bifunctional dCMP-dCTP deaminase shows dCMP deaminase and dCTP deaminase activities, dCTP serves as a positive heterotropic effector for the dCMP deaminase activity and a positive homotropic effector for the dCTP deaminase activity, and the enzymatic efficiency of the dCMP deaminase activity is about four times higher than that of the dCTP deaminase activity, the same active site is involved in both dCMP and dCTP deaminations
-
-
?
additional information
?
-
-
substrate specificity
-
-
?
additional information
?
-
-
substrate specificity
-
-
?
additional information
?
-
-
halogenated deoxycytidylates: very poor substrates
-
-
?
additional information
?
-
Tequatrovirus T4
-
function of enzyme is that of enhancing stability of T4-phage multienzyme complex involved in dNTP synthesis, the complex is less stable in cd mutants
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
5-methyl-dCMP + H2O
dTMP + NH3
-
deamination pathway, overview
-
-
?
dCTP + H2O
dUTP + NH3
-
-
-
?
deoxycytidine + H2O
deoxyuridine + NH3
-
the enzyme is a key enzyme in the inactivation of deoxycytidine and several chemotherapeutically important nucleoside analogues
-
-
?
additional information
?
-
dCMP + H2O
dUMP + NH3
-
providing an adequate supply of dUMP for formation of dTMP, via dTMP synthase a crucial intermediate for synthesis of DNA
-
-
?
dCMP + H2O
dUMP + NH3
-
importance in the generation of dUMP, precursor for synthesis of thymidine nucleotides
-
?
dCMP + H2O
dUMP + NH3
-
importance in the generation of dUMP, precursor for synthesis of thymidine nucleotides
-
-
?
dCMP + H2O
dUMP + NH3
-
importance in the generation of dUMP, precursor for synthesis of thymidine nucleotides
-
?
dCMP + H2O
dUMP + NH3
Betapolyomavirus macacae
-
providing an adequate supply of dUMP for formation of dTMP, via dTMP synthase a crucial intermediate for synthesis of DNA
-
-
?
dCMP + H2O
dUMP + NH3
Betapolyomavirus macacae
-
importance in the generation of dUMP, precursor for synthesis of thymidine nucleotides
-
-
?
dCMP + H2O
dUMP + NH3
-
enzyme involved in main pathway leading to synthesis of dTTP, role in provision of precursors for DNA synthesis
-
-
?
dCMP + H2O
dUMP + NH3
-
enzyme involved in main pathway leading to synthesis of dTTP, role in provision of precursors for DNA synthesis
-
?
dCMP + H2O
dUMP + NH3
-
-
-
?
dCMP + H2O
dUMP + NH3
-
providing an adequate supply of dUMP for formation of dTMP, via dTMP synthase a crucial intermediate for synthesis of DNA
-
-
?
dCMP + H2O
dUMP + NH3
-
importance in the generation of dUMP, precursor for synthesis of thymidine nucleotides
-
-
?
dCMP + H2O
dUMP + NH3
-
providing an adequate supply of dUMP for formation of dTMP, via dTMP synthase a crucial intermediate for synthesis of DNA
-
-
?
dCMP + H2O
dUMP + NH3
-
importance in the generation of dUMP, precursor for synthesis of thymidine nucleotides
-
-
?
dCMP + H2O
dUMP + NH3
-
-
-
?
dCMP + H2O
dUMP + NH3
-
-
-
?
dCMP + H2O
dUMP + NH3
-
-
-
?
dCMP + H2O
dUMP + NH3
-
-
-
?
dCMP + H2O
dUMP + NH3
-
-
-
?
dCMP + H2O
dUMP + NH3
-
catalyzed reaction is at a branching point of the pathway leading to dCTP and dTTP, immediate pyrimidine deoxynucleotide precursors in DNA biosynthesis
-
?
dCMP + H2O
dUMP + NH3
-
providing an adequate supply of dUMP for formation of dTMP, via dTMP synthase a crucial intermediate for synthesis of DNA
-
-
?
dCMP + H2O
dUMP + NH3
-
importance in the generation of dUMP, precursor for synthesis of thymidine nucleotides
-
-
?
dCMP + H2O
dUMP + NH3
-
-
-
-
?
dCMP + H2O
dUMP + NH3
-
-
-
?
dCMP + H2O
dUMP + NH3
-
true substrate probably dCMP-Mg complex
-
-
?
dCMP + H2O
dUMP + NH3
-
major function to provide supplementary route for dTMP synthesis, located at a branch point in pyrimidine metabolic pathway
-
-
?
dCMP + H2O
dUMP + NH3
-
enzyme involved in formation of dTMP
-
-
?
dCMP + H2O
dUMP + NH3
-
providing an adequate supply of dUMP for formation of dTMP, via dTMP synthase a crucial intermediate for synthesis of DNA
-
-
?
dCMP + H2O
dUMP + NH3
-
enzyme within pyrimidine deoxynucleotide pathway
-
-
?
dCMP + H2O
dUMP + NH3
-
enzyme plays an important if not rate controlling step in cell division
-
-
?
dCMP + H2O
dUMP + NH3
-
regulating dTMP for DNA synthesis
-
-
?
dCMP + H2O
dUMP + NH3
-
pathway for synthesis of dUMP, nucleotide required for synthesis of dTMP
-
-
?
dCMP + H2O
dUMP + NH3
-
importance in the generation of dUMP, precursor for synthesis of thymidine nucleotides
-
-
?
dCMP + H2O
dUMP + NH3
-
-
-
-
?
dCMP + H2O
dUMP + NH3
-
major function to provide supplementary route for dTMP synthesis, located at a branch point in pyrimidine metabolic pathway
-
-
?
dCMP + H2O
dUMP + NH3
-
enzyme involved in formation of dTMP
-
-
?
dCMP + H2O
dUMP + NH3
-
providing an adequate supply of dUMP for formation of dTMP, via dTMP synthase a crucial intermediate for synthesis of DNA
-
-
?
dCMP + H2O
dUMP + NH3
-
enzyme within pyrimidine deoxynucleotide pathway
-
-
?
dCMP + H2O
dUMP + NH3
-
enzyme plays an important if not rate controlling step in cell division
-
-
?
dCMP + H2O
dUMP + NH3
-
regulating dTMP for DNA synthesis
-
-
?
dCMP + H2O
dUMP + NH3
-
importance in the generation of dUMP, precursor for synthesis of thymidine nucleotides
-
-
?
dCMP + H2O
dUMP + NH3
Herpes simplex virus
-
enzyme involved in main pathway leading to synthesis of dTTP, role in provision of precursors for DNA synthesis
-
-
?
dCMP + H2O
dUMP + NH3
Herpes simplex virus
-
providing an adequate supply of dUMP for formation of dTMP, via dTMP synthase a crucial intermediate for synthesis of DNA
-
-
?
dCMP + H2O
dUMP + NH3
Herpes simplex virus
-
importance in the generation of dUMP, precursor for synthesis of thymidine nucleotides
-
-
?
dCMP + H2O
dUMP + NH3
-
-
-
-
?
dCMP + H2O
dUMP + NH3
-
-
-
?
dCMP + H2O
dUMP + NH3
-
-
-
-
?
dCMP + H2O
dUMP + NH3
-
enzyme contributes formation of DNA by providing dUMP as substrate for thymidylate synthase. Potential role in cell division
-
?
dCMP + H2O
dUMP + NH3
-
providing an adequate supply of dUMP for formation of dTMP, via dTMP synthase a crucial intermediate for synthesis of DNA
-
-
?
dCMP + H2O
dUMP + NH3
-
key enzyme in pyrimidine deoxyribonucleotide metabolism, catalyzing the rate-limiting step, plays an important role in providing a balanced supply of dCTP and dTTP for DNA synthesis
-
?
dCMP + H2O
dUMP + NH3
-
important role in regulation of intracellular TTP levels
-
-
ir
dCMP + H2O
dUMP + NH3
-
importance in the generation of dUMP, precursor for synthesis of thymidine nucleotides
-
-
?
dCMP + H2O
dUMP + NH3
-
enzyme is major source of mammalian cell dUMP pool and regulates the synthesis of TMP
-
-
ir
dCMP + H2O
dUMP + NH3
-
the enzyme is important in the de novo synthesis of thymidine nucleotides, and may also be involved in the metabolism of anticancer agents
-
-
?
dCMP + H2O
dUMP + NH3
-
the enzyme plays a key role in the thymidylate synthase, pathway overview
-
-
?
dCMP + H2O
dUMP + NH3
-
-
-
-
?
dCMP + H2O
dUMP + NH3
-
one of the regulatory enzymes of DNA biosynthesis
-
?
dCMP + H2O
dUMP + NH3
-
enzyme of pyrimidine deoxyribonucleotide metabolism, important for the synthesis of thymidine nucleotides from thymine. Enzyme could provide the major means of dUMP formation
-
?
dCMP + H2O
dUMP + NH3
-
-
-
-
?
dCMP + H2O
dUMP + NH3
the enzyme is crucial to the production of dTTP needed for DNA replication and damage repair. It mediates dNTP pool balance required for normal chloroplast development and vegetative growth in plants
-
-
?
dCMP + H2O
dUMP + NH3
-
dUMP is a key intermediate in the synthesis of dTTP and subsequently of DNA synthesis
-
?
dCMP + H2O
dUMP + NH3
-
providing an adequate supply of dUMP for formation of dTMP, via dTMP synthase a crucial intermediate for synthesis of DNA
-
-
?
dCMP + H2O
dUMP + NH3
-
importance in the generation of dUMP, precursor for synthesis of thymidine nucleotides
-
-
?
dCMP + H2O
dUMP + NH3
-
providing an adequate supply of dUMP for formation of dTMP, via dTMP synthase a crucial intermediate for synthesis of DNA
-
-
?
dCMP + H2O
dUMP + NH3
-
importance in the generation of dUMP, precursor for synthesis of thymidine nucleotides
-
-
?
dCMP + H2O
dUMP + NH3
-
-
-
-
?
dCMP + H2O
dUMP + NH3
-
providing an adequate supply of dUMP for formation of dTMP, via dTMP synthase a crucial intermediate for synthesis of DNA
-
-
?
dCMP + H2O
dUMP + NH3
-
importance in the generation of dUMP, precursor for synthesis of thymidine nucleotides
-
-
?
dCMP + H2O
dUMP + NH3
-
deoxycytidine nucleotides are shunted into dTMP biosynthetic pathway via reaction catalyzed by dCMP deaminase
-
?
dCMP + H2O
dUMP + NH3
-
-
-
-
?
dCMP + H2O
dUMP + NH3
-
-
-
-
?
dCMP + H2O
dUMP + NH3
-
the regulation signal is transmitted by Arg4 from the allosteric site to the active site via modifications in the interactions at the interface where the substrate binding pocket is involved and the relocations of Arg26, His65, Tyr120, and Arg121 to envelope the active site in order to stabilize substrate binding in the complex, allosteric mechanism for enzyme regulation, regulator binding structure, overview
-
-
?
dCMP + H2O
dUMP + NH3
Tequatrovirus T4
-
-
-
-
?
dCMP + H2O
dUMP + NH3
Tequatrovirus T4
-
major function to provide supplementary route for dTMP synthesis, located at a branch point in pyrimidine metabolic pathway
-
-
?
dCMP + H2O
dUMP + NH3
Tequatrovirus T4
-
providing an adequate supply of dUMP for formation of dTMP, via dTMP synthase a crucial intermediate for synthesis of DNA
-
-
?
dCMP + H2O
dUMP + NH3
Tequatrovirus T4
-
critical branchpoint in pyrimidine deoxynucleotide metabolism
-
-
?
dCMP + H2O
dUMP + NH3
Tequatrovirus T4
-
importance in the generation of dUMP, precursor for synthesis of thymidine nucleotides
-
-
?
dCMP + H2O
dUMP + NH3
Tequatrovirus T4
-
the enzyme is the major supplier of substrate for the thymidylate synthase important in DNA synthesis
-
-
?
additional information
?
-
-
-
-
-
?
additional information
?
-
-
enzyme is involved in elimination of some dCyd analogues, such as 2',2'-difluorodeoxycytidine, fluoro-dCyd, and ara-C, from tissues or cultured cells
-
-
?
additional information
?
-
-
the enzyme also performs 5-methylcytidyl-DNA deaminase activity but to a lesser extent, deamination pathway, overview
-
-
?
additional information
?
-
-
the human cytomegalovirus requires the cellular host dCMP deaminase for replication in quiescent cells, overview
-
-
?
additional information
?
-
Tequatrovirus T4
-
function of enzyme is that of enhancing stability of T4-phage multienzyme complex involved in dNTP synthesis, the complex is less stable in cd mutants
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
metal ion
-
required, in the absence of metal ions: 40% activity, Zn2+ highest activity, around 20% activity with Co2+, Mn2+, and Ni2+, no activity in the presence of Mg2+, Ca2+ and Fe2+
Ca2+
-
no activation
Ca2+
-
reaction completely dependent on divalent cations, most effective Mg2+, Mn2+ and Ca2+ less effective than Mg2+
Ca2+
-
divalent cations not necessary to improve activity, activation by dCTP and inhibition by dTTP both show an absolute requirement for a divalent cation. Mg2+, Ca2+ and Mn2+ almost equally effective in promoting activation by dCTP, Cu2+, Zn2+, and Co2+ are ineffective
Ca2+
-
activation of enzyme by dCTP or inhibition by TTP has absolute requirement for the presence of a divalent cation. Mg2+, Ca2+ and Mn2+ are equally effective cations for enzyme activation by dCTP, Mn2+ and Ca2+ are more effective than Mg2+ in enzyme inhibition by TTP
Ca2+
can partially substitute for Mg2+
Ca2+
-
can partially substitute for Mg2+, 50% of the activity with Mg2+
Co2+
-
metal ion required, highest activity with Zn2+, about 20% activity with Co2+, Mn2+, and Ni2+
Co2+
-
divalent cations not necessary to improve activity, activation by dCTP and inhibition by dTTP both show an absolute requirement for a divalent cation. Mg2+, Ca2+ and Mn2+ almost equally effective in promoting activation by dCTP, Cu2+, Zn2+, and Co2+ are ineffective
divalent cations
-
reaction completely dependent on divalent cations
divalent cations
-
divalent cations not necessary to improve activity, activation by dCTP and inhibition by dTTP both show an absolute requirement for a divalent cation. Mg2+, Ca2+ and Mn2+ almost equally effective in promoting activation by dCTP, Cu2+, Zn2+, and Co2+ are ineffective
Mg2+
-
no activation
Mg2+
-
enzyme requires dCTP, Zn2+, and 2-mercaptoethanol, Mg2+ cannot substitute for Zn2+
Mg2+
-
required for complete activation by dCTP
Mg2+
-
dCTP activation and dTTP inhibition requires presence of Mg2+ or Mn2+
Mg2+
-
required for activation by dCTP and for inhibition by dTTP
Mg2+
-
required for activation by dCTP, Mg2+ in the absence of dCTP: no effect
Mg2+
-
required for activation by dCTP and for inhibition by dTTP
Mg2+
-
required for activation by dCTP and for inhibition by dTTP
Mg2+
-
required for activation by dCTP and for inhibition by dTTP
Mg2+
-
reaction completely dependent on divalent cations, most effective Mg2+, Mn2+ and Ca2+ less effective than Mg2+
Mg2+
Herpes simplex virus
-
required for complete activation by dCTP
Mg2+
Herpes simplex virus
-
required for activation by dCTP and for inhibition by dTTP
Mg2+
-
divalent cations not necessary to improve activity, activation by dCTP and inhibition by dTTP both show an absolute requirement for a divalent cation. Mg2+, Ca2+ and Mn2+ almost equally effective in promoting activation by dCTP, Cu2+, Zn2+, and Co2+ are ineffective
Mg2+
-
required for activation by dCTP and for inhibition by dTTP
Mg2+
-
activation of enzyme by dCTP or inhibition by TTP has absolute requirement for the presence of a divalent cation. Mg2+, Ca2+ and Mn2+ are equally effective cations for enzyme activation by dCTP, Mn2+ and Ca2+ are more effective than Mg2+ in enzyme inhibition by TTP
Mg2+
-
required for activation by dCTP, Mg2+ in the absence of dCTP: no effect
Mg2+
the enzyme depends on divalent cations, Mg2+ is preferred, but can partially be substituted by Ca2+, Ni2+, or Mn2+
Mg2+
-
required for activation by dCTP, Mg2+ in the absence of dCTP: no effect
Mg2+
-
required for activity, in complex with activator dCTP, can be substituted by Ca2+ or Mn2+, but not by Zn2+, Co2+, Ni2+, and Cu2+
Mg2+
Tequatrovirus T4
-
-
Mg2+
Tequatrovirus T4
-
required for activation by dCTP and for inhibition by dTTP
Mn2+
-
metal ion required, highest activity with Zn2+, about 20% activity with Co2+, Mn2+, and Ni2+
Mn2+
-
dCTP activation and dTTP inhibition requires presence of Mg2+ or Mn2+
Mn2+
-
reaction completely dependent on divalent cations, most effective Mg2+, Mn2+ and Ca2+ less effective than Mg2+
Mn2+
-
divalent cations not necessary to improve activity, activation by dCTP and inhibition by dTTP both show an absolute requirement for a divalent cation. Mg2+, Ca2+ and Mn2+ almost equally effective in promoting activation by dCTP, Cu2+, Zn2+, and Co2+ are ineffective
Mn2+
-
activation of enzyme by dCTP or inhibition by TTP has absolute requirement for the presence of a divalent cation. Mg2+, Ca2+ and Mn2+ are equally effective cations for enzyme activation by dCTP, Mn2+ and Ca2+ are more effective than Mg2+ in enzyme inhibition by TTP
Mn2+
can partially substitute for Mg2+
Mn2+
-
the enzyme shows dependence on Zn2+ and Mg2+ for correct folding and activity
Mn2+
-
can partially substitute for Mg2+, 26% of the activity with Mg2+
Ni2+
-
metal ion required, highest activity with Zn2+, about 20% activity with Co2+, Mn2+, and Ni2+
Ni2+
can partially substitute for Mg2+
Zn2+
-
required, enzyme requires dCTP, Zn2+, and 2-mercaptoethanol, Mg2+ cannot substitute for Zn2+
Zn2+
-
divalent cations not necessary to improve activity, activation by dCTP and inhibition by dTTP both show an absolute requirement for a divalent cation. Mg2+, Ca2+ and Mn2+ almost equally effective in promoting activation by dCTP, Cu2+, Zn2+, and Co2+ are ineffective
Zn2+
the enzyme contains a zinc binding site, but shows less than 6% of maximal activity with Zn2+ as divalent cation
Zn2+
-
the enzyme shows dependence on Zn2+ and Mg2+ for correct folding and activity
Zn2+
-
two conserved motifs are involved in the binding of Zn2+, Zn2+ cannot substitute for Mg2+
Zn2+
Tequatrovirus T4
-
two resident Zn2+ atoms /subunit
Zn2+
Tequatrovirus T4
-
involved in catalytic mechanism involving His104, and Cys132 and Cys135
Zn2+
Tequatrovirus T4
-
the enzyme shows a zinc ion-based reaction mechanism, binding structure and coordinating residues, two zinc ions per subunit, the first is coordinated by His104, Cys132, and Cys135, the second by Cys19, Cys49, His94, and Glu102, overview
additional information
-
metal ion required, in the absence of metal ions: 40% activity, Zn2+ highest activity, around 20% activity with Co2+, Mn2+, and Ni2+, no activity in the presence of Mg2+, Ca2+ and Fe2+
additional information
-
metal ions not required
additional information
bacteriophage SP8
-
metal ions not required
additional information
-
metal ions not required
additional information
-
bound bivalent metal cations probably present
additional information
-
metal ions not required
additional information
-
no metals required
additional information
-
divalent cations not necessary to improve activity, activation by dCTP and inhibition by dTTP both show an absolute requirement for a divalent cation. Mg2+, Ca2+ and Mn2+ almost equally effective in promoting activation by dCTP, Cu2+, Zn2+, and Co2+ are ineffective
additional information
-
Co2+, Ni2+, and Cu2+ cannot substitute for Mg2+
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
(4Z,7Z,10Z,13Z,16Z,19Z)-henicosa-4,7,10,13,16,19-hexaenoic acid
-
decreases the enzyme activity in vivo in transformed NM-16 fibroblasts, but increases it in normal Rat-2 fibroblasts
2',2'-difluoro-dCTP
-
dual mechanism of inhibition; partial purified enzyme
2',2'-difluorodeoxycytidine
-
high cellular nucleotide levels: inhibition: Concentration-dependent inhibition; inhibition by nucleotides of 2',2'-difluorodeoxycytidine, dual mechanism of inhibition
2',3'-di-dTTP
-
7% inhibition
2',3'-dihydro-2',3'-dideoxy-thymidine monophosphate
-
-
2'-beta-D-deoxyribose-pyrimidin-2-one 5'-phosphate
-
competitive inhibition, Ki: 0.000012 mM
2'-deoxythymidine
-
0.05 mM: 60% inhibition, 0.01 mM: 5% inhibition; dThd
3'-azido-2',3'-dideoxy-thymidine monophosphate
-
-
3,4,5,6-tetrahydro-2'-deoxyuridine
3,4,5,6-tetrahydro-2'-dUMP
5-(acryloylamino-pentanol-1)2'-beta-D-dUMP
-
-
5-bromo-dUTP
-
92% inhibition
5-Hg-dUMP
-
potent inhibitor, 0.0001 mM: 54% inhibition
AMP
-
slight inhibition, competitive inhibitor, mechanism of inhibition
arabinosylcytosine
-
ara-C, deactivating dCMP deaminase
beta-D-2',2'-difluorodeoxycytidine
-
competitive
beta-L-2',3'-dideoxy-3'-thiacytidine monophosphate
-
slight inhibition
beta-L-2',3'-dideoxy-5'-fluoro-3'-thiacytidine monophosphate
-
-
Co2+
-
1 mM: 75% inhibition
Cu2+
-
1 mM: 98% inhibition
D-beta-2'-fluoro-5-methyl-arabinofuranosyluracil monophosphate
-
no inhibition by L-beta-2'-fluoro-5-methyl-arabinofuranosyluracil monophosphate
deoxytetrahydrouridine
-
-
deoxythymidine 5'-triphosphate
Tequatrovirus T4
-
allosteric feedback regulation of the enzyme by products of the metabolic pathway, allosteric regulation involved residues 112 and 115
glycerol
-
substrate dCMP: activation, substrate dCMP-Hg-S-CH2-CH2-OH, 20% glycerol: inhibition, removed by dTTP
guanidine hydrochloride
-
0.5 M: 50% inhibition, 1 M: 100% inhibition
H4dUMP
inhibits both dCTP and dCMP deaminase activities
Herpes antiserum
Herpes simplex virus
-
inhibition of Herpes infected-cell enzyme
-
Hg(CH3COO)2
-
Hg(acetate)2; mercuroacetate, potent inhibitor, 0.0002 mM: 26% inhibition, 0.001 mM: 40% inhibition
PDRP
inhibits both dCTP and dCMP deaminase activities
pre-immune serum
Herpes simplex virus
-
inhibition of Herpes infected-cell enzyme
-
pyrimidin-2-one deoxyribotide
Tequatrovirus T4
-
active site binding structure, overview
TDP
-
slight inhibition, competitive inhibitor, mechanism of inhibition
tetrahydrodeoxyuridine
-
-
UTP-adipic hydrazide
-
cooperative inhibition, reversed by dCTP
Zn2+
-
1 mM: 94% inhibition
3,4,5,6-tetrahydro-2'-deoxyuridine
-
-
3,4,5,6-tetrahydro-2'-deoxyuridine
-
specifc inhibition
3,4,5,6-tetrahydro-2'-dUMP
-
high-affinity inhibitor, rapid, reversible inhibition, kinetics of inhibition. At low substrate or competitor concentrations: activation. Tetrahydro-dUMP activates enzyme at low substrate concentrations
3,4,5,6-tetrahydro-2'-dUMP
-
specific potent inhibitor
3,4,5,6-tetrahydro-2'-dUMP
-
potent inhibitor, Ki: 0.00001 mM
5-Hg-dCMP
-
irreversible competitive inhibitor, in the absence of mercaptoethanol
5-Hg-dCMP
-
potent inhibitor, in the absence of 2-mercaptoethanol, 0.00024 mM: 37% inhibition, 0.0024 mM: 70% inhibition
dAMP
-
competitive inhibitor, activates enzyme at low substrate concentration mimicing the cooperative effect of the substrate
dAMP
-
at low substrate, dCMP, concentration: activation at higher concentration of either: competitive inhibition; competitive inhibitor, activates enzyme at low substrate concentration mimicing the cooperative effect of the substrate
dAMP
-
competitive inhibitor, activates enzyme at low substrate concentration mimicing the cooperative effect of the substrate
dAMP
-
kinetics of dAMP inhibition
dCTP
-
allosteric activator, 12fold, for aminohydrolysis of dCMP, inhibitor, 50% inhibition, for substrate: 5-Hg-dCMP in the presence of mercaptoethanol, dCMP-Hg-S-CH2-CH2-OH
dCTP
-
allosteric activator, 12fold, for aminohydrolysis of dCMP, inhibitor, 50% inhibition, for substrate: 5-Hg-dCMP in the presence of mercaptoethanol, dCMP-Hg-S-CH2-CH2-OH
dGMP
-
competitive inhibitor
dGMP
-
effective competitive inhibitor, mechanism of inhibition
dGMP
-
potent competitive inhibitor
dTTP
bacteriophage SP8
-
no inhibition by dTTP
dTTP
-
enzyme from Herpes simplex virus infected BHK-21/C13 cells from baby-hamster is more resistant to inhibition by dTTP than enzyme from non-infected cells
dTTP
-
allosteric inhibitor; maximum inhibition at 0.015 mM, inhibition requires presence of Mg2+ or Mn2+
dTTP
-
allosteric inhibitor
dTTP
-
0.01 mM: 100% inhibition. In the presence of glutaraldehyde enzyme is less sensitive to inhibitory effect of dTTP; allosteric inhibitor; inhibited form: dTTP-enzyme; kinetics of inhibition
dTTP
-
allosteric inhibitor
dTTP
-
allosteric inhibitor; allosteric inhibitor, 100% inhibition, for substrate dCMP, becomes activator, 2.5fold, for mercury substrate: 5-Hg-dCMP in the presence of mercaptoethanol, dCMP-Hg-S-CH2-CH2-OH
dTTP
-
allosteric inhibitor; cooperative inhibition, reversed by dCTP, mechanism of inhibition
dTTP
-
allosteric inhibitor
dTTP
-
allosteric inhibitor
dTTP
-
allosteric inhibitor; inhibition by dTTP increases markedly as the pH is raised, pH-dependent inhibition
dTTP
-
allosteric inhibitor
dTTP
-
allosteric inhibitor; dTTP favores disaggregated or inhibited state
dTTP
-
allosteric inhibitor
dTTP
-
allosteric inhibitor; dTTP favores disaggregated or inhibited state
dTTP
Herpes simplex virus
-
enzyme from Herpes simplex virus infected BHK-21/C13 cells from baby-hamster is more resistant to inhibition by dTTP than enzyme from non-infected cells
dTTP
Herpes simplex virus
-
-
dTTP
-
allosteric inhibitor
dTTP
-
0.0015 mM: 50% inhibition. dTTP inhibition reversed by dCTP; allosteric inhibitor
dTTP
-
allosteric inhibitor
dTTP
-
inhibits dCMPD by allosteric interaction
dTTP
-
allosteric inhibitor; specifically markedly inhibited, Ki: 0.0017 mM
dTTP
-
allosteric inhibitor
dTTP
inhibits dCMP deamination, feedback inhibition
dTTP
-
allosteric inhibitor
dTTP
-
allosteric inhibitor
dTTP
-
an allosteric inhibitor
dTTP
Tequatrovirus T4
-
allosteric inhibitor
dTTP
Tequatrovirus T4
-
allosteric inhibitor; wild-type, 0.1 mM dTTP: 20% inhibition, mutants R115E and R115Q: 0.1 mM dTTP: no inhibition. 0.3 mM dTTP: wild-type 90% inhibition, mutants R115E and R115Q: 30% inhibition
dTTP
Tequatrovirus T4
-
allosteric inhibitor; mutant F112A, no or very slight inhibition, high concentration, 0.24 mM: slight inhibition
dTTP
Tequatrovirus T4
-
allosteric inhibitor
dUMP
-
competitive inhibition, kinetics and mechanism of inhibition
dUMP
-
competitive inhibitor
dUMP
-
poor competitive inhibitor, mechanism of inhibition
dUMP
-
product inhibition
dUMP
-
potent competitive inhibitor
dUMP
Tequatrovirus T4
-
-
dUTP
-
slight inhibition
dUTP
Tequatrovirus T4
-
allosteric inhibitor
EDTA
-
completely
EDTA
-
1 mM: 90% inhibition, restored to maximum by 1-5 mM Mg2+ or Mn2+
EDTA
-
no inhibition by EDTA
EDTA
Tequatrovirus T4
-
-
Glutaraldehyde
-
25% glutaraldehyde: rapid inhibition; dCTP protects enzyme to a certain extent. Glutaraldehyde fixes dCMPase in the activated conformation induced by dCTP
Glutaraldehyde
-
25% glutaraldehyde: rapid inhibition; substrate dCMP: rapid inhibition, protection by dGMP, or dTTP or both is slight, substrate dCMP-Hg-S-CH2-CH2-OH: 3fold activation
GMP
-
slight inhibition
GMP
-
potent competitive inhibitor
TMP
-
poor competitive inhibitor, mechanism of inhibition
TTP
-
activating enzyme up to 1.7fold at concentration of 0.00025 mM, but inhibiting at higher concentration, 0.015 mM: 85% inhibition; inhibition by TTP has absolute requirement for the presence of a divalent cation, Mn2+ and Ca2+ are more effective than Mg2+ in the inhibition of enzyme activity by TTP; TTP inhibition partially reversed by dCTP
TTP
-
inhibits dCMPD by allosteric interaction
additional information
-
no inhibition by KCl up to 0.3 M
-
additional information
-
mechanism of inhibition
-
additional information
-
mechanism of inhibition
-
additional information
-
kinetics of inhibition; mechanism of inhibition
-
additional information
Herpes simplex virus
-
no inhibition by KCl up to 0.3 M
-
additional information
-
no inhibition by EDTA
-
additional information
-
little or no inhibition by 3,4,5,6-tetrahydrouridine; mechanism of inhibition
-
additional information
-
no inhibition by deoxynucleotide triphosphates
-
additional information
-
inhibitory potency of pyrimidine phosphate analogues, overview
-
additional information
-
monophosphates of deoxycytidine analogs nucleosides in the L-conformation (e.g. apricitabine, lamivudine, and emtricitabine) and dideoxycytidine do not act as inhibitors of dCMPD
-
additional information
-
no inhibition by dATP, dGTP, 5-bromo-UTP
-
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(4Z,7Z,10Z,13Z,16Z,19Z)-henicosa-4,7,10,13,16,19-hexaenoic acid
-
increases the enzyme activity in vivo in normal Rat-2 fibroblasts, but decreases it in transformed NM-16 fibroblasts
1-beta-D-arabinofuranosylcytosine 5'-triphosphate
-
low activation, allosteric activator
2-mercaptoethanol
-
enzyme requires dCTP, Zn2+, and 2-mercaptoethanol
3,4,5,6-tetrahydro-2'-dUMP
-
high-affinity inhibitor, rapid, reversible inhibition, kinetics of inhibition. At low substrate or competitor concentrations: activation. Tetrahydro-dUMP activates enzyme at low substrate concentrations
ammonium sulfate
-
substrate dCMP-Hg-S-CH2-CH2-OH, 0.2 M ammonium sulfate: activation, reversed by cCTP
CDP
-
low activation, allosteric activator
dCMP
-
allosterically increases dCMPD activity
deoxycytidine 5'-triphosphate
Tequatrovirus T4
-
allosteric feedback regulation of the enzyme by products of the metabolic pathway, allosteric regulation involved residues 112 and 115
dGTP
-
very slight activation
Glutaraldehyde
-
substrate dCMP: rapid inhibition, protection by dGMP, or dTTP or both is slight, substrate dCMP-Hg-S-CH2-CH2-OH: 3fold activation
glycerol
-
substrate dCMP: activation, substrate dCMP-Hg-S-CH2-CH2-OH, 20% glycerol: inhibition
Herpes antiserum
-
activation of host-cell enzyme
-
pre-immune serum
-
activation of host-cell enzyme, twice as effective as Herpes antiserum
-
TTP
-
activating enzyme up to 1.7fold at concentration of 0.00025 mM, but inhibiting at higher concentration, 0.015 mM: 85% inhibition
5-hydroxymethyl-dCTP
-
natural positive allosteric effector, enzyme is much more effectively regulated by its natural effector, 5-hydroxymethyl-dCTP, than by dCTP, binding of 5-hydroxymethyl-dCTP is much more pH dependent than dCTP
5-hydroxymethyl-dCTP
-
natural positive allosteric effector, enzyme is much more effectively regulated by its natural effector, 5-hydroxymethyl-dCTP, than by dCTP, binding of 5-hydroxymethyl-dCTP is much more pH dependent than dCTP
5-hydroxymethyl-dCTP
-
pH-dependent activation
5-hydroxymethyl-dCTP
-
30% activation
5-hydroxymethyl-dCTP
Tequatrovirus T4
-
natural positive allosteric effector, enzyme is much more effectively regulated by its natural effector, 5-hydroxymethyl-dCTP, than by dCTP, binding of 5-hydroxymethyl-dCTP is much more pH dependent than dCTP
5-hydroxymethyl-dCTP
Tequatrovirus T4
-
pH-dependent activation
5-hydroxymethyl-dCTP
Tequatrovirus T4
-
natural activator, required for activity
CTP
-
no activation
CTP
-
low activation, allosteric activator
dAMP
-
competitive inhibitor, activates enzyme at low substrate concentration mimicing the cooperative effect of the substrate, dAMP activates dCMPase 4fold at low substrate concentration, no activating effect on the modified enzyme, glutaraldehyde-dCMPase
dAMP
-
at low substrate, dCMP, concentration: activation at higher concentration of either: inhibition
dAMP
-
0.025 mM: low activation
dCDP
-
100% activation
dCDP
-
low activation, allosteric activator
dCTP
-
-
dCTP
-
required, enzyme requires dCTP, Zn2+, and 2-mercaptoethanol
dCTP
-
dCTP not required and not activared by dCTP
dCTP
bacteriophage SP8
-
dCTP not required and not activared by dCTP
dCTP
-
positive allosteric effector
dCTP
-
maximum activation at 0.04 mM, dCTP activation requires presence of Mg2+ or Mn2+
dCTP
-
enzyme from Herpes simplex virus infected BHK-21/C13 cells from baby-hamster: maximum activation is achieved by lower concentration of dCTP than of enzyme from non-infected cells
dCTP
-
positive allosteric effector
dCTP
-
severalfold activation, activation requires presence of Mg2+
dCTP
-
allosteric activator
dCTP
-
positive allosteric effector
dCTP
-
activate form: dCTP-enzyme
dCTP
-
allosteric activator, 12fold, for aminohydrolysis of dCMP, inhibitor, 50% inhibition, for substrate: 5-Hg-dCMP in the presence of mercaptoethanol, dCMP-Hg-S-CH2-CH2-OH
dCTP
-
allosteric activator, increases substrate affinity
dCTP
-
glutaraldehyde fixes dCMPase in the activated conformation induced by dCTP
dCTP
-
dCMPase is activated 15.3fold, glutaraldehyde-dCMPase only 1.8fold
dCTP
-
positive allosteric effector
dCTP
-
activation by dCTP decreases markedly as the pH is increased, pH-dependent activation
dCTP
-
dCTP promotes an aggregation of enzyme subunits to the active state
dCTP
-
severalfold activation, activation requires presence of Mg2+
dCTP
-
pH-dependent activation
dCTP
-
positive allosteric effector
dCTP
-
activation by dCTP is reversed by addition of dTTP
dCTP
-
dCTP promotes an aggregation of enzyme subunits to the active state
dCTP
Herpes simplex virus
-
-
dCTP
Herpes simplex virus
-
enzyme from Herpes simplex virus infected BHK-21/C13 cells from baby-hamster: maximum activation is achieved by lower concentration of dCTP than of enzyme from non-infected cells
dCTP
-
allosteric activator
dCTP
-
positive allosteric effector
dCTP
-
activation by dCTP is reversed by addition of dTTP
dCTP
-
less than 0.001 mM: very high activation
dCTP
-
activation by dCTP has absolute requirement for the presence of a divalent cation, Mg2+, Ca2+ and Mn2+ are equally effective cations for enzyme activation by dCTP
dCTP
-
positive allosteric effector
dCTP
-
specifically markedly activated, Km: 0.00017 mM
dCTP
-
activation, absolute requirement for dCTP
dCTP
activates dCMP deamination about 7fold at 0.005-0.1 mM
dCTP
-
positive allosteric effector
dCTP
-
severalfold activation, activation requires presence of Mg2+
dCTP
-
positive allosteric effector
dCTP
-
an allosteric activator, in complex with Mg2+
dCTP
Tequatrovirus T4
-
required for activity
dCTP
Tequatrovirus T4
-
positive allosteric effector
dCTP
Tequatrovirus T4
-
hexameric form of enzyme is activated by dCTP, while the dimer is not
dCTP
Tequatrovirus T4
-
wild-type, dCTP required for activity, no activity in absence of dCTP and Mg2+
dCTP
Tequatrovirus T4
-
mutant F112A, dCTP not required
dCTP
Tequatrovirus T4
-
severalfold activation, activation requires presence of Mg2+
dCTP
Tequatrovirus T4
-
mutants R115E and R115Q: dCTP not required. R115Q: slight, 30-40% activation by 0.02 mM dCTP
dTTP
-
no activation
dTTP
-
allosteric inhibitor, 100% inhibition, for substrate dCMP, becomes activator, 2.5fold, for mercury substrate: 5-Hg-dCMP in the presence of mercaptoethanol, dCMP-Hg-S-CH2-CH2-OH, at relatively high, 1 to 2 mM, dCMP-Hg-S-CH2-CH2-OH concentration
dTTP
-
allosteric inhibitor, 100% inhibition, for substrate dCMP, becomes activator, 2.5fold, for mercury substrate: 5-Hg-dCMP in the presence of mercaptoethanol, dCMP-Hg-S-CH2-CH2-OH, at relatively high, 1 to 2 mM, dCMP-Hg-S-CH2-CH2-OH concentration
additional information
-
no activation by ATP, UTP, CTP and dTTP
-
additional information
-
no activation by KCl up to 0.3 M
-
additional information
-
mechanism of activation
-
additional information
-
mechanism of activation
-
additional information
Herpes simplex virus
-
no activation by KCl up to 0.3 M
-
additional information
-
infection with the human cytomegalovirus upregulates the enzyme involved in thymidylate synthesis especially the dCMP deaminase, but not the dUTPase, overview
-
additional information
-
no activation by deoxynucleotide triphosphates
-
additional information
Tequatrovirus T4
-
the phage dCMP deaminase expression is increased upon infection of Escherichia coli
-
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Adenocarcinoma
Gemcitabine sensitivity-related mRNA expression in endoscopic ultrasound-guided fine-needle aspiration biopsy of unresectable pancreatic cancer.
Adenocarcinoma
Intratumoural expression of deoxycytidylate deaminase or ribonuceotide reductase subunit M1 expression are not related to survival in patients with resected pancreatic cancer given adjuvant chemotherapy.
Aortic Valve Stenosis
Nonmyocardial production of ST2 protein in human hypertrophy and failure is related to diastolic load.
Asthma
Elevated soluble ST2 protein levels in sera of patients with asthma with an acute exacerbation.
Asthma
Expression and function of the ST2 gene in a murine model of allergic airway inflammation.
Asthma
ST2 expression and release by the bronchial epithelium is downregulated in asthma.
Autoimmune Diseases
Identification of human ST2 protein in the sera of patients with autoimmune diseases.
Brain Neoplasms
Sensitization to X ray by 5-chloro-2'-deoxycytidine co-administered with tetrahydrouridine in several mammalian cell lines and studies of 2'-chloro derivatives.
Breast Neoplasms
An association between RRM1 haplotype and gemcitabine-induced neutropenia in breast cancer patients.
Breast Neoplasms
Quantification of chemotherapeutic target gene mRNA expression in human breast cancer biopsies: comparison of real-time reverse transcription-PCR vs. relative quantification reverse transcription-PCR utilizing DNA sequencer analysis of PCR products.
Carcinogenesis
Identification of novel cellular targets in biliary tract cancers using global gene expression technology.
Carcinoma, Ehrlich Tumor
Deoxycytidylate aminohydrolase in cells of Ehrlich ascites tumour.
Carcinoma, Hepatocellular
Activities of some enzymes of pyrimidine and DNA synthesis in a rat transplantable hepatoma and human primary hepatomas, in cell lines derived from these tissues, and in human fetal liver.
Carcinoma, Hepatocellular
Correlation of deoxycytidylate deaminase activity with cell proliferation during hepatocarcinogenesis and tumour growth in transplantable rat hepatomas.
Carcinoma, Hepatocellular
Deoxycytidylate deaminase and related enzymes of thymidine triphosphate metabolism in hepatomas and precancerous rat liver.
Carcinoma, Hepatocellular
Deoxycytidylate Deaminase and Thymine Catabolic Activity of Transplanted Mouse and Rat Hepatomas and their Histology.
Carcinoma, Hepatocellular
Factors affecting deoxycytidylate deaminase activity in some transplantable rat hepatomas.
Carcinoma, Hepatocellular
Nucleotide interconversions. IV. Activities of deoxycytidylate deaminase and thymidylate synthetase in normal rat liver and hepatomas.
Carcinoma, Hepatocellular
The comparative enzymology and cell origin of rat hepatomas. I. Deoxycytidylate deaminase and thymine degradation.
Cardiomyopathy, Dilated
Nonmyocardial production of ST2 protein in human hypertrophy and failure is related to diastolic load.
Cysts
The influence of the nucleus on the regulation of the dCMP deaminase in acetabularia.
dcmp deaminase deficiency
Imbalanced deoxyribonucleoside triphosphate pools and spontaneous mutation rates determined during dCMP deaminase-defective bacteriophage T4 infections.
dcmp deaminase deficiency
Mutational specificity of DNA precursor pool imbalances in yeast arising from deoxycytidylate deaminase deficiency or treatment with thymidylate.
dcmp deaminase deficiency
Phage T4-induced dTTP accretion bolsters a tRNase-based host defense.
dcmp deaminase deficiency
Replication Fork Collapse and Genome Instability in dCMP Deaminase Mutant.
Dengue
Elevated levels of soluble ST2 protein in dengue virus infected patients.
Eclampsia
Serum deoxycytidylate deaminase as an index of high-risk pregnancy.
Essential Hypertension
Serum deoxycytidylate deaminase as an index of high-risk pregnancy.
Glioma
Gene Expression and Methylation Analyses Suggest DCTD as a Prognostic Factor in Malignant Glioma.
Heart Diseases
ST2 protein in heart disease: from discovery to mechanisms and prognostic value.
Heart Failure
Soluble ST2 protein and hospitalizations due to worsening chronic heart failure during a one-year follow-up in a population with reduced ejection fraction.
Heart Failure
Soluble ST2 protein in chronic heart failure is independent of traditional factors.
Heart Failure, Systolic
Soluble ST2 protein in the short-term prognosis after hospitalisation in chronic systolic heart failure.
Herpes Simplex
Aanlysis of dCMP deaminase and CDP reductase levels in hamster cells infected by herpes simplex virus.
Herpes Simplex
Deoxycytidylate deaminase evidence for a new enzyme in cells infected by the virus of herpes simplex.
Herpes Simplex
The effect of 5-iodo-2'-deoxyuridine 5'-triphosphate, an allosteric inhibitor, on deoxycytidylate deaminase "induced" by herpes simplex virus.
Idiopathic Pulmonary Fibrosis
The increase in serum soluble ST2 protein upon acute exacerbation of idiopathic pulmonary fibrosis.
Infections
Aanlysis of dCMP deaminase and CDP reductase levels in hamster cells infected by herpes simplex virus.
Infections
Relationship between Escherichia coli B titer and the level of deoxycytidylate deaminase activity induced on bacteriophage T2r + infection.
Leukemia
Deoxycytidylate deaminase activity in lymphoproliferative disorders.
Leukemia
Deoxycytidylate deaminase activity in non-stimulated and phytohemagglutinin-stimulated human lymphocytes, and in leukemic cells.
Leukemia
Kinetic behaviour and allosteric regulation of human deoxycytidylate deaminase derived from leukemic cells.
Leukemia
Modulation of deoxycytidylate deaminase in intact human leukemia cells. Action of 2',2'-difluorodeoxycytidine.
Leukemia
Therapeutic response of leukemic mice treated with fluorinated pyrimidines and inhibitors of deoxyuridylate synthesis.
Leukemia, Lymphocytic, Chronic, B-Cell
Deoxycytidylate deaminase activity in non-stimulated and phytohemagglutinin-stimulated human lymphocytes, and in leukemic cells.
Leukemia, Monocytic, Acute
Deoxycytidylate deaminase activity in non-stimulated and phytohemagglutinin-stimulated human lymphocytes, and in leukemic cells.
Leukemia, Myeloid, Acute
Gene expression of hENT1, dCK, CDA, dCMPD and topoisomerase II? as an indicator of chemotherapy response in AML treated with cytarabine and daunorubicin.
Liver Neoplasms, Experimental
Deoxycytidylate aminohydrolase activity in the Novikoff hepatoma is dependent on the localization of the tumor.
Liver Neoplasms, Experimental
Factors affecting deoxycytidylate deaminase activity in some transplantable rat hepatomas.
Lung Injury
IL-1 R-related protein ST2 suppressed the initial stage of bleomycin-induced lung injury.
Lymphoma
Deoxycytidylate deaminase activity in lymphoproliferative disorders.
Lymphoma, Non-Hodgkin
Intracellular metabolism of the new antiviral compound 1-(S)-[3-hydroxy-2-(phosphonomethoxy)propyl]-5-azacytosine.
Lymphoproliferative Disorders
Deoxycytidylate deaminase activity in lymphoproliferative disorders.
Myocardial Infarction
Deoxycytidylate deaminase in the diagnosis of acute myocardial infarction.
Myocardial Infarction
Serum deoxycytidylate deaminase activity after myocardial infarction.
Nasal Polyps
[Expression and role of IL-33 and its receptor ST2 in eosinophilic and non-eosinophilic chronic rhinosinusitis with nasal polyps].
Neoplasms
Activation of deoxycytidylate deaminase from certain embryonal and tumour tissues by 6-aza-2'-deoxycytidine-5'-monophosphate and 2'-deoxycytidine-5'-triphosphate.
Neoplasms
Activities of various enzymes of pyrimidine nucleotide and DNA syntheses in normal and neoplastic human tissues.
Neoplasms
Circulating proteins as predictors of cardiovascular mortality in end-stage renal disease.
Neoplasms
Correlation of deoxycytidylate deaminase activity with cell proliferation during hepatocarcinogenesis and tumour growth in transplantable rat hepatomas.
Neoplasms
Deoxycytidylate aminohydrolase activity in the Novikoff hepatoma is dependent on the localization of the tumor.
Neoplasms
Deoxycytidylate aminohydrolase in cells of Ehrlich ascites tumour.
Neoplasms
Deoxycytidylate deaminase activity in lymphoproliferative disorders.
Neoplasms
Differences in biomarker concentrations and predictions of long-term outcome in patients with ST-elevation and non-ST-elevation myocardial infarction.
Neoplasms
Effect of Magnesium Supplementation on Circulating Biomarkers of Cardiovascular Disease.
Neoplasms
Enzyme-Driven Chemo-and Radiation-Therapy with 12 Pyrimidine Nucleoside Analogs Not Yet in the Clinic.
Neoplasms
Five-chlorodeoxycytidine, a tumor-selective enzyme-driven radiosensitizer, effectively controls five advanced human tumors in nude mice.
Neoplasms
Identification of novel cellular targets in biliary tract cancers using global gene expression technology.
Neoplasms
In vitro and in vivo radiation sensitization by the halogenated pyrimidine 5-chloro-2'-deoxycytidine.
Neoplasms
INCREASED STABILITY OF TEMPLATES FOR SOME ENZYMES OF DNA SYNTHESIS IN TUMORS: DEOXYCYTIDYLATE DEAMINASE, THYMIDINE AND THYMIDYLATE KINASE.
Neoplasms
Metabolism of pyrimidine nucleotides in various tissues and tumor cells from rodents.
Neoplasms
Modulation of deoxycytidylate deaminase in intact human leukemia cells. Action of 2',2'-difluorodeoxycytidine.
Neoplasms
Protective, tumor-selective dual pathway activation of 5-fluoro-2'-deoxycytidine provided by tetrahydrouridine in mice bearing mammary adenocarcinoma-755.
Neoplasms
Sensitization to X ray by 5-chloro-2'-deoxycytidine co-administered with tetrahydrouridine in several mammalian cell lines and studies of 2'-chloro derivatives.
Neoplasms
The effect of ST2 gene product on anchorage-independent growth of a glioblastoma cell line, T98G.
Neoplasms
Tumor-selective metabolism of 5-fluoro-2'-deoxycytidine coadministered with tetrahydrouridine compared to 5-fluorouracil in mice bearing Lewis lung carcinoma.
Neoplasms
Use of 5-fluorodeoxycytidine and tetrahydrouridine to exploit high levels of deoxycytidylate deaminase in tumors to achieve DNA- and target-directed therapies.
Nephrotic Syndrome
Potential role of soluble ST2 protein in idiopathic nephrotic syndrome recurrence following kidney transplantation.
Non-ST Elevated Myocardial Infarction
Differences in biomarker concentrations and predictions of long-term outcome in patients with ST-elevation and non-ST-elevation myocardial infarction.
Pancreatic Neoplasms
Intratumoural expression of deoxycytidylate deaminase or ribonuceotide reductase subunit M1 expression are not related to survival in patients with resected pancreatic cancer given adjuvant chemotherapy.
Placental Insufficiency
Serum deoxycytidylate deaminase as an index of high-risk pregnancy.
Pleural Effusion, Malignant
Expression of ST2 in helper T lymphocytes of malignant pleural effusions.
Pneumonia
ST2 protein induced by inflammatory stimuli can modulate acute lung inflammation.
Pre-Eclampsia
Plasma urate and serum deoxycytidylate deaminase measurements for the early diagnosis of pre-eclampsia.
Pre-Eclampsia
Serum deoxycytidylate deaminase as an index of high-risk pregnancy.
Precursor Cell Lymphoblastic Leukemia-Lymphoma
Deoxycytidylate deaminase activity in non-stimulated and phytohemagglutinin-stimulated human lymphocytes, and in leukemic cells.
Pregnancy, High-Risk
Serum deoxycytidylate deaminase as an index of high-risk pregnancy.
Sarcoma, Avian
Induction of deoxycytidylate deaminase and uridine kinase and activation of cellular DNA synthesis in the course of transformation of chicken embryo cells infected by Rous sarcoma virus in vitro.
Sepsis
Increased levels of soluble ST2 protein and IgG1 production in patients with sepsis and trauma.
ST Elevation Myocardial Infarction
Differences in biomarker concentrations and predictions of long-term outcome in patients with ST-elevation and non-ST-elevation myocardial infarction.
Stomach Neoplasms
Decreased ST2 expression is associated with gastric cancer progression and pathogenesis.
Subarachnoid Hemorrhage
Elevation of ST2 protein levels in cerebrospinal fluid following subarachnoid hemorrhage.
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0.1
5-methyl-dCMP
-
pH 8.3, in the presence of dCTP or 5-hydroxymethyl-dCTP. Decreasing the pH to 6.0 lowers the apparent Km
0.26
dCMP-Hg-S-CH2-CH2-OH
-
-
0.347 - 0.467
deoxycytidine
additional information
additional information
-
0.5
CMP
-
pH 6.0
10
CMP
-
pH 8.3, in the presence of dCTP or 5-hydroxymethyl-dCTP. Decreasing the pH to 6.0 lowers the apparent Km
12
CMP
-
pH 8.0, absence of effector
0.021
dCMP
Tequatrovirus T4
-
wild-type, presence of dCTP and Mg2+
0.021
dCMP
Tequatrovirus T4
-
recombinant wild-type enzyme, pH 8.0, 30°C
0.024
dCMP
-
in the presence of dCTP
0.037
dCMP
-
in the presence of dCTP
0.1
dCMP
-
pH 8.3, in the presence of dCTP or 5-hydroxymethyl-dCTP. Decreasing the pH to 6.0 lowers the apparent Km
0.127
dCMP
Tequatrovirus T4
-
R115Q, absence of dCTP
0.127
dCMP
Tequatrovirus T4
-
recombinant mutant R115Q, pH 8.0, 30°C
0.137
dCMP
Tequatrovirus T4
-
R115E, absence of dCTP
0.137
dCMP
Tequatrovirus T4
-
recombinant mutant R115E, pH 8.0, 30°C
0.32
dCMP
Herpes simplex virus
-
in the presence of dCTP and presence of 2 mM Mg2+
0.32
dCMP
-
in the presence of dCTP and presence of 2 mM Mg2+
0.76
dCMP
pH 9.5, 42°C, in presence of 0.1 mM dCTP
0.91
dCMP
Herpes simplex virus
-
in the presence of dCTP and absence of 2 mM Mg2+
0.91
dCMP
-
in the presence of dCTP and absence of 2 mM Mg2+
0.347
deoxycytidine
-
pH 8.1, 37°C, Rat-2 fibroblasts in presence of 0.02 mM docosahexanoic acid
0.349
deoxycytidine
-
pH 8.1, 37°C, Rat-2 fibroblasts
0.462
deoxycytidine
-
pH 8.1, 37°C, NM-16 fibroblasts
0.467
deoxycytidine
-
pH 8.1, 37°C, NM-16 fibroblasts in presence of 0.02 mM docosahexanoic acid
additional information
additional information
-
-
-
additional information
additional information
-
-
-
additional information
additional information
-
-
-
additional information
additional information
-
-
-
additional information
additional information
-
-
-
additional information
additional information
-
-
-
additional information
additional information
-
-
-
additional information
additional information
Tequatrovirus T4
-
-
-
additional information
additional information
-
-
-
additional information
additional information
-
-
-
additional information
additional information
-
-
-
additional information
additional information
-
-
-
additional information
additional information
-
kinetics
-
additional information
additional information
-
kinetics of inhibition
-
additional information
additional information
-
kinetic properties and parameters
-
additional information
additional information
-
kinetic properties and parameters
-
additional information
additional information
-
kinetic behavior and data
-
additional information
additional information
Tequatrovirus T4
-
kinetic analysis, data and characterization of wild-type and mutants R115E, R115Q and F112A
-
additional information
additional information
-
kinetic studies, steady-state kinetics, allosteric kinetics
-
additional information
additional information
-
kinetic experiments
-
additional information
additional information
-
kinetic experiments
-
additional information
additional information
-
preliminary kinetic studies and kinetic data
-
additional information
additional information
-
preliminary kinetic studies and kinetic data
-
additional information
additional information
-
cooperative kinetics, kinetic affinities and effects, mechanism
-
additional information
additional information
-
cooperative kinetics, kinetic affinities and effects, mechanism
-
additional information
additional information
-
steady-state kinetic Hill coefficients, allosteric enzyme kinetic data
-
additional information
additional information
-
Km-value of modified enzyme, glutaraldehyde-dCMPase, is lower than that of native enzyme
-
additional information
additional information
-
kinetic data, kinetics typical of activated enzyme
-
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2-mercaptoethanol stabilizes
dCTP and Mg2+ stabilize
-
dCTP and MgCl2 stabilize
-
dCTP or glycerol stabilize
dCTP stabilizing hexameric state, dTTP destabilizing
dCTP, 0.125 mM, or ethyleneglycol, 20% v/v, stabilize
-
dCTP, or to a lesser extent dTTP, stabilize against heat inactivation, protein denaturants and proteolytic enzymes
-
dithiothreitol, 2 mM, stabilizes
-
EDTA: denaturation, EDTA removes the two resident Zn2+ atoms /subunit
Tequatrovirus T4
-
enzyme extremely labile, stabilized by dCTP /Mg2+
-
enzyme very heat-labile, stabilized against heat inactivation by dCTP, 0.125 mM and ethyleneglycol, 20% v/v
-
enzyme very sensitive to preliminary heating, dCTP, 0.125 mM, and Mg2+, 2 mM, stabilize against heat inactivation
ethyleneglycol prevents protein denaturation during freezing in the presence of 2-mercaptoethanol
-
ethyleneglycol, 10-20% stabilizes
-
ethyleneglycol, 20%, stabilizes against thermal and UV inactivation and denaturation factors with different mechanism of action
-
glycerol, 15%, stabilizes
-
guanidine hydrochloride, 1 M, complete loss in activity accompanied by disaggregation of enzyme, 6 M: denaturation, complete reactivation with 50 mM 2-mercaptoethanol
-
guanidine-HCl, 6 M, denaturation, complete restoration of activity on removal of denaturant by dilution or dialysis
Tequatrovirus T4
-
some free nucleotides, most effective dCTP, stabilize
-
thiols, such as 2-mercaptoethanol or dithiothreitol, stabilize, in the absence of thiols the enzyme is highly unstable
-
Zn2+ or dCTP stabilize, protect against inactivation
-
2-mercaptoethanol stabilizes
-
2-mercaptoethanol stabilizes
-
2-mercaptoethanol stabilizes
-
2-mercaptoethanol stabilizes
Tequatrovirus T4
-
2-mercaptoethanol stabilizes
-
dCTP or glycerol stabilize
-
dCTP or glycerol stabilize
-
dCTP stabilizes
-
dCTP stabilizes
Tequatrovirus T4
-
dCTP stabilizing hexameric state, dTTP destabilizing
-
dCTP stabilizing hexameric state, dTTP destabilizing
-
enzyme is unstable
-
enzyme very sensitive to preliminary heating, dCTP, 0.125 mM, and Mg2+, 2 mM, stabilize against heat inactivation
Herpes simplex virus
-
enzyme very sensitive to preliminary heating, dCTP, 0.125 mM, and Mg2+, 2 mM, stabilize against heat inactivation
-
glycerol stabilizes
-
thiols stabilize
-
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Neale, G.A.M.; Mitchell, A.; Finch, L.R.
Enzymes of pyrimidine deoxyribonucleotide metabolism in Mycoplasma mycoides subsp. mycoides
J. Bacteriol.
156
1001-1005
1983
Mycoplasma mycoides
brenda
Maley, F.; Belfort, M.; Maley, G.
Probing the infra-structure of thymidylate synthase and deoxycytidylate deaminase
Adv. Enzyme Regul.
22
413-430
1984
Escherichia phage T2, Gallus gallus
brenda
Nucci, R.; Raia, C.A.; Vaccaro, C.; Sepe, S.; Scarano, E.; Rossi, M.
Freezing of dCMP aminohydrolase in the activated conformation by glutaraldehyde
J. Mol. Biol.
124
133-145
1978
Equus asinus
brenda
Ellims, P.H.; Kao, A.Y.; Chabner, B.A.
Deoxycytidylate deaminase. Purification and some properties of the enzyme isolated from human spleen
J. Biol. Chem.
256
6335-6340
1981
Homo sapiens
brenda
McIntosh, E.M.; Haynes, R.H.
Isolation of a Saccharomyces cerevisiae mutant strain deficient in deoxycytidylate deaminase activity and partial characterization of the enzyme
J. Bacteriol.
158
644-649
1984
Saccharomyces cerevisiae, no activity in Escherichia coli, no activity in Salmonella typhimurium
brenda
Mastrantonio, S.; Nucci, R.; Vaccaro, C.; Rossi, M.; Whitehead, E.P.
Analysis of competition for substrate sites in an allosteric enzyme with co-operative kinetics. Effects of dAMP and dUMP on donkey spleen deoxycytidylate aminohydrolase
Eur. J. Biochem.
137
421-427
1983
Equus asinus
brenda
Raia, C.A.; Nucci, R.; Vaccaro, C.; Sepe, S.; Rella, R.
Reversal of the effect of the allosteric ligands of dCMP-aminohydrolase and stabilization of the enzyme in the T form
J. Mol. Biol.
157
557-570
1982
Equus asinus
brenda
Maley, G.F.
Deoxycytidylate deaminase from T2-infected Escherichia coli
Methods Enzymol.
51
412-418
1978
Escherichia phage T2, no activity in Escherichia coli
brenda
Mollgaard, H.; Neuhard, J.
Deoxycytidylate deaminase from Bacillus subtilis. Purification, characterization, and physiological function
J. Biol. Chem.
253
3536-3542
1978
Bacillus subtilis, Bacillusphage phiE, bacteriophage SP8, no activity in Escherichia coli, no activity in Salmonella typhimurium, Bacillus subtilis ED40
brenda
Dosseva.I.M.; Tomov, T.H.
Stabilizing effect of ethyleneglycol on deoxycytidylate deaminase in relation to thermal and ultraviolet inactivation
Dokl. Bolg. Akad. Nauk
28
241-244
1975
Mus musculus
-
brenda
Rolton, H.A.; Keir, H.M.
Deoxycytidylate deaminase. Evidence for a new enzyme in cells infected by the virus of Herpes simplex
Biochem. J.
143
403-409
1974
Cricetinae, Herpes simplex virus
brenda
Rolton, H.A.; Keir, H.M.
Deoxycytidylate deaminase. Properties of the enzyme from cultured kidney cells of baby hamster
Biochem. J.
141
211-217
1974
Cricetinae
brenda
Maley, G.F.; Guarino, D.U.
T2r+ bacteriophage-induced enzymes. I. The purification and properties of deoxycytidylate deaminase
J. Biol. Chem.
247
931-939
1972
Escherichia phage T2, Tequatrovirus T4, Gallus gallus
brenda
Maley, G.F.; MacColl, R.; Maley, F.
T2r+ bacteriophage-induced enzymes. II. The subunit structure of deoxycytidylate deaminase
J. Biol. Chem.
247
940-945
1972
Escherichia phage T2, Gallus gallus
brenda
Sergott, R.C.; Debeer, L.J.; Bessman, M.J.
On the regulation of a bacterial deoxycytidylate deaminase
J. Biol. Chem.
246
7755-7758
1971
Lactobacillus acidophilus, Staphylococcus aureus
brenda
Nucci, R.; Raia, C.A.; Vaccaro, C.; Rossi, M.; Whitehead, E.P.
Allosteric modifier and substrate binding of donkey deoxycytidylate aminohydrolase (EC 3.5.4.12)
Arch. Biochem. Biophys.
289
19-25
1991
Equus asinus
brenda
Whitehead, E.P.; Nucci, R.; Vaccaro, C.; Rossi, M
Hill coefficient ratios give binding ratios of allosteric enzyme effectors; inhibition, activation, and squatting in deoxycytidylate aminohydrolase (EC 3.5.4.12)
Arch. Biochem. Biophys.
289
12-18
1991
Equus asinus
brenda
Nucci, R.; Febbraio, F.; Piccialli, G.; de Napoli, L.; Vaccaro, C.; Rossi, M.; Whitehead, E.P.
Interaction of the high-affinity inhibitor tetrahydro-dUMP with the allosteric enzyme deoxycytidylate aminohydrolase
Arch. Biochem. Biophys.
310
49-53
1994
Equus asinus
brenda
Keefe, R.G.; Maley, G.F.; Saxl, R.L.; Maley, F.
A T4-phage deoxycytidylate deaminase mutant that no longer requires deoxycytidine 5'-triphosphate for activation
J. Biol. Chem.
275
12598-12602
2000
Escherichia phage T2, Tequatrovirus T4, Enterobacteria phage T6
brenda
Xu, Y.Z.; Plunkett, W.
Modulation of deoxycytidylate deaminase in intact human leukemia cells. Action of 2',2'-difluorodeoxycytidine
Biochem. Pharmacol.
44
1819-1827
1992
Homo sapiens
brenda
Moore, J.T.; Ciesla, J.M.; Changchien, L.M.; Maley, G.F.; Maley, F.
Identification of a site necessary for allosteric regulation in T4-phage deoxycytidylate deaminase
Biochemistry
33
2104-2112
1994
Tequatrovirus T4, Homo sapiens
brenda
Maley, G.F.; Lobo, A.P.; Maley, F.
Properties of an affinity-column-purified human deoxycytidylate deaminase
Biochim. Biophys. Acta
1162
161-170
1993
Homo sapiens
brenda
Riva, C.M.; Barra, Y.; Cano, J.P.; Tubiana, N.; Lejeune, C.; Merlin, M.; de Sousa, G.; Carcassonne, Y.; Kreis, W.; Lovecchio, J.; Rottachi, C.; Rustum, Y.M.
Correlation between deoxycytidine kinase and deaminase activities and response to therapy with ARA-C
J. Cell. Pharmacol.
1
79-85
1990
Homo sapiens
-
brenda
Maley, F.; Maley, G.F.
A tale of two enzymes, deoxycytidylate deaminase and thymidylate synthase
Prog. Nucleic Acid Res. Mol. Biol.
39
49-80
1990
Bacillus subtilis, Escherichia phage T2, Tequatrovirus T4, Enterobacteria phage T6, Saccharomyces cerevisiae, Gallus gallus, Equus asinus, Herpes simplex virus, Homo sapiens, no activity in Escherichia coli, no activity in Salmonella typhimurium, Polyomavirus sp., Rattus norvegicus, Echinoidea, Betapolyomavirus macacae
brenda
Cha, M.C.; Meckling-Gill, K.A.
Modifications of deoxycytidine kinase and deaminase activities by docosahexaenoic acid in normal and transformed rat fibroblasts
Biochem. Pharmacol.
63
717-723
2002
Rattus norvegicus
brenda
Almog, R.; Maley, F.; Maley, G.F.; Maccoll, R.; Van Roey, P.
Three-dimensional structure of the R115E mutant of T4-bacteriophage 2-deoxycytidylate deaminase
Biochemistry
43
13715-13723
2004
Tequatrovirus T4
brenda
Jost, J.P.; Thiry, S.; Siegmann, M.
5-Methyldeoxycytidine monophosphate deaminase and 5-methylcytidyl-DNA deaminase activities are present in human mature sperm cells
FEBS Lett.
519
128-134
2002
Homo sapiens
brenda
Gribaudo, G.; Riera, L.; Caposio, P.; Maley, F.; Landolfo, S.
Human cytomegalovirus requires cellular deoxycytidylate deaminase for replication in quiescent cells
J. Gen. Virol.
84
1437-1441
2003
Homo sapiens
brenda
Liou, J.Y.; Krishnan, P.; Hsieh, C.C.; Dutschman, G.E.; Cheng, Y.C.
Assessment of the effect of phosphorylated metabolites of anti-human immunodeficiency virus and anti-hepatitis B virus pyrimidine analogs on the behavior of human deoxycytidylate deaminase
Mol. Pharmacol.
63
105-110
2003
Homo sapiens
brenda
Liskay, R.M.; Wheeler, L.J.; Mathews, C.K.; Erdeniz, N.
Involvement of deoxycytidylate deaminase in the response to Sn1-type methylation DNA damage in budding yeast
Curr. Biol.
17
R755-R757
2007
Saccharomyces cerevisiae
brenda
Hou, H.F.; Liang, Y.H.; Li, L.F.; Su, X.D.; Dong, Y.H.
Crystal structures of Streptococcus mutans 2-deoxycytidylate deaminase and its complex with substrate analog and allosteric regulator dCTP x Mg2+
J. Mol. Biol.
377
220-231
2008
Streptococcus mutans
brenda
Zhang, Y.; Maley, F.; Maley, G.F.; Duncan, G.; Dunigan, D.D.; Van Etten, J.L.
Chloroviruses encode a bifunctional dCMP-dCTP deaminase that produces two key intermediates in dTTP formation
J. Virol.
81
7662-7671
2007
Paramecium bursaria Chlorella virus 1 (O41078), Paramecium bursaria Chlorella virus 1
brenda
Li, L.S.; Morales, J.C.; Veigl, M.; Sedwick, D.; Greer, S.; Meyers, M.; Wagner, M.; Fishel, R.; Boothman, D.A.
DNA mismatch repair (MMR)-dependent 5-fluorouracil cytotoxicity and the potential for new therapeutic targets
Br. J. Pharmacol.
158
679-692
2009
Homo sapiens
brenda
Jansen, R.S.; Rosing, H.; Schellens, J.H.; Beijnen, J.H.
Deoxyuridine analog nucleotides in deoxycytidine analog treatment: secondary active metabolites?
Fundam. Clin. Pharmacol.
25
172-185
2011
Homo sapiens
brenda
Marx, A.; Alian, A.
The first crystal structure of a dTTP-bound deoxycytidylate deaminase validates and details the allosteric-inhibitor binding site
J. Biol. Chem.
290
682-690
2015
Cyanophage S-TIM5 (H6WFU3)
brenda
Xu, J.; Deng, Y.; Li, Q.; Zhu, X.; He, Z.
STRIPE2 encodes a putative dCMP deaminase that plays an important role in chloroplast development in rice
J. Genet. Genomics
41
539-548
2014
Oryza sativa
brenda
Li, Y.; Guo, Z.; Jin, L.; Wang, D.; Gao, Z.; Su, X.; Hou, H.; Dong, Y.
Mechanism of the allosteric regulation of Streptococcus mutans 2-deoxycytidylate deaminase
Acta Crystallogr. Sect. D
72
883-891
2016
Streptococcus mutans (Q8DSE5), Streptococcus mutans ATCC 700610 (Q8DSE5)
brenda
Scortecci, J.F.; Serrao, V.H.B.; Cheleski, J.; Torini, J.R.; Romanello, L.; DeMarco, R.; DMuniz Pereira, H.
Spectroscopic and calorimetric assays reveal dependence on dCTP and two metals (Zn2++Mg2+) for enzymatic activity of Schistosoma mansoni deoxycytidylate (dCMP) deaminase
Biochim. Biophys. Acta
1865
1326-1335
2017
Schistosoma mansoni
brenda
Niu, M.; Wang, Y.; Wang, C.; Lyu, J.; Wang, Y.; Dong, H.; Long, W.; Wang, D.; Kong, W.; Wang, L.; Guo, X.; Sun, L.; Hu, T.; Zhai, H.; Wang, H.; Wan, J.
ALR encoding dCMP deaminase is critical for DNA damage repair, cell cycle progression and plant development in rice
J. Exp. Bot.
68
5773-5786
2017
Oryza sativa (Q5JN09), Oryza sativa
brenda