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1-[3-(2,5-dioxo-1-phenylimidazolidin-4-yl)propyl]guanidine + H2O
N-[3-(2,5-dioxo-1-phenylimidazolidin-4-yl)propyl]urea + NH3
-
-
-
?
1-[3-(2,5-dioxo-2,3,4,5-tetrahydro-1H-1,4-benzodiazepin-3-yl)propyl]guanidine + H2O
N-[[6-(benzyloxy)-1H-indol-2-yl]methyl]urea + NH3
-
-
-
?
1-[3-(2,5-dioxoimidazolidin-4-yl)propyl]guanidine + H2O
N-[3-(2,5-dioxoimidazolidin-4-yl)propyl]urea + NH3
-
-
-
?
1-[3-[1-(biphenyl-3-ylmethyl)-2,5-dioxoimidazolidin-4-yl]propyl]guanidine + H2O
N-(3-[1-[([1,1'-biphenyl]-3-yl)methyl]-2,5-dioxoimidazolidin-4-yl]propyl)urea + NH3
-
-
-
?
1-[[6-(benzyloxy)-1H-indol-2-yl]methyl]guanidine + H2O
N-[[6-(benzyloxy)-1H-indol-2-yl]methyl]urea + NH3
-
-
-
?
2-acetyl-Gly-Arg-Gly-Lys-Gly-Gly-Lys-Gly-Leu-Gly-Lys-Gly-Gly-Ala-Lys + H2O
2-acetyl-Gly-Cit-Gly-Lys-Gly-Gly-Lys-Gly-Leu-Gly-Lys-Gly-Gly-Ala-Lys + NH3
-
-
-
-
?
3-acetyl-Arg-Gly-Lys-Gly-Gly-Lys-Gly-Leu-Gly-Lys-Gly-Gly-Ala-Lys + H2O
3-acetyl-Cit-Gly-Lys-Gly-Gly-Lys-Gly-Leu-Gly-Lys-Gly-Gly-Ala-Lys + NH3
-
-
-
-
?
4-carbamimidamido-N-(diphenylmethyl)butanamide + H2O
4-(carbamoylamino)-N-(diphenylmethyl)butanamide + NH3
-
-
-
?
4-[4-(3-carbamimidamidopropyl)-2,5-dioxoimidazolidin-1-yl]-2-ethoxy-N-(4-methoxyphenyl)benzamide + H2O
4-[4-[3-(carbamoylamino)propyl]-2,5-dioxoimidazolidin-1-yl]-2-ethoxy-N-(4-methoxyphenyl)benzamide + NH3
-
-
-
?
4-[4-(3-carbamimidamidopropyl)-2,5-dioxoimidazolidin-1-yl]-N-(4-methoxyphenyl)benzamide + H2O
4-[4-[3-(carbamoylamino)propyl]-2,5-dioxoimidazolidin-1-yl]-N-(4-methoxyphenyl)benzamide + NH3
-
-
-
?
4-[4-(3-carbamimidamidopropyl)-2,5-dioxoimidazolidin-1-yl]-N-phenylbenzamide + H2O
4-[4-[3-(carbamoylamino)propyl]-2,5-dioxoimidazolidin-1-yl]-N-phenylbenzamide + NH3
-
-
-
?
4-[4-[3-(carbamoylamino)propyl]-2,5-dioxoimidazolidin-1-yl]-N-(4-methoxyphenyl)benzamide + H2O
4-[4-[3-(carbamoylamino)propyl]-2,5-dioxoimidazolidin-1-yl]-2-methoxy-N-(4-methoxyphenyl)benzamide + NH3
-
-
-
?
Ac-Ala-Gly-Arg-Gly-Lys + H2O
Ac-Ala-Gly-Cit-Gly-Lys + NH3
-
-
-
-
?
Ac-Ser-Ala-Arg-Gly-Lys + H2O
Ac-Ser-Ala-Cit-Gly-Lys + NH3
-
-
-
-
?
Ac-Ser-Gly-Ala-Gly-Lys-Gly-Gly-Lys-Gly-Leu-Gly-Lys-Gly-Gly-Ala-Ala-Arg-His-Arg-Lys-Val + H2O
?
-
-
-
-
?
Ac-Ser-Gly-Arg-Ala-Lys + H2O
Ac-Ser-Gly-Cit-Ala-Lys + NH3
-
-
-
-
?
Ac-Ser-Gly-Arg-Gly-acetyl-Lys-Gly-Gly-acetyl-Lys-Gly-Leu-Gly-acetyl-Lys-Gly-Gly-Ala-acetyl-Lys + H2O
?
-
-
-
-
?
Ac-Ser-Gly-Arg-Gly-acetyl-Lys-Gly-Gly-acetyl-Lys-Gly-Leu-Gly-acetyl-Lys-Gly-Gly-Ala-acetyl-Lys-Arg-His-Arg-acetyl-Lys-Val + H2O
?
-
-
-
-
?
Ac-Ser-Gly-Arg-Gly-Ala + H2O
Ac-Ser-Gly-Cit-Gly-Ala + NH3
-
-
-
-
?
Ac-Ser-Gly-Arg-Gly-Lys + H2O
Ac-Ser-Gly-Cit-Gly-Lys + NH3
-
-
-
-
?
Ac-Ser-Gly-Arg-Gly-Lys-Gly-Gly-Lys-Gly-Leu-Gly-Lys-Gly + H2O
Ac-Ser-Gly-Cit-Gly-Lys-Gly-Gly-Lys-Gly-Leu-Gly-Lys-Gly + NH3
-
-
-
-
?
Ac-Ser-Gly-Arg-Gly-Lys-Gly-Gly-Lys-Gly-Leu-Gly-Lys-Gly-Gly-Ala + H2O
Ac-Ser-Gly-Cit-Gly-Lys-Gly-Gly-Lys-Gly-Leu-Gly-Lys-Gly-Gly-Ala + NH3
-
-
-
-
?
Ac-Ser-Gly-Arg-Gly-Lys-Gly-Gly-Lys-Gly-Leu-Gly-Lys-Gly-Gly-Ala-Ala-Arg-His-Arg-Ala-Val + H2O
?
-
-
-
-
?
Ac-Ser-Gly-Arg-Gly-Lys-Gly-Gly-Lys-Gly-Leu-Gly-Lys-Gly-Gly-Ala-Ala-Arg-His-Arg-Lys-Val + H2O
?
-
-
-
-
?
Ac-Ser-Gly-Arg-Gly-Lys-Gly-Gly-Lys-Gly-Leu-Gly-Lys-Gly-Gly-Ala-Lys + H2O
Ac-Ser-Gly-Cit-Gly-Lys-Gly-Gly-Lys-Gly-Leu-Gly-Lys-Gly-Gly-Ala-Lys + NH3
-
-
-
-
?
Ac-Ser-Gly-Arg-Gly-Lys-Gly-Gly-Lys-Gly-Leu-Gly-Lys-Gly-Gly-Ala-Lys-Ala-His-Arg-Ala-Val + H2O
?
-
-
-
-
?
Ac-Ser-Gly-Arg-Gly-Lys-Gly-Gly-Lys-Gly-Leu-Gly-Lys-Gly-Gly-Ala-Lys-Arg-His + H2O
?
-
-
-
-
?
Ac-Ser-Gly-Arg-Gly-Lys-Gly-Gly-Lys-Gly-Leu-Gly-Lys-Gly-Gly-Ala-Lys-Arg-His-Ala-Ala-Val + H2O
?
-
-
-
-
?
Ac-Ser-Gly-Arg-Gly-Lys-Gly-Gly-Lys-Gly-Leu-Gly-Lys-Gly-Gly-Ala-Lys-Arg-His-Arg-Lys-Val + H2O
?
-
-
-
-
?
Ac-Ser-Gly-Arg-Gly-Lys-Gly-Gly-Lys-Gly-Leu-Gly-Lys-Gly-Gly-Ala-Lys-Lys-His-Lys-Ala-Val + H2O
Ac-Ser-Gly-Cit-Gly-Lys-Gly-Gly-Lys-Gly-Leu-Gly-Lys-Gly-Gly-Ala-Lys-Lys-His-Lys-Ala-Val + NH3
-
-
-
-
?
acety-L-Arg + H2O
acetyl-L-citrulline + NH3
-
-
-
-
?
acetyl-L-Arg + H2O
acetyl-L-citrulline + NH3
acetyl-L-Arg methyl ester + H2O
acetyl-L-citrulline methyl ester + NH3
agmatine + H2O
? + NH3
-
5.4% activity compared to L-arginine
-
-
?
aldolase 1A L-Arg + H2O
aldolase 1A L-citrulline + NH3
-
-
-
?
aldolase 3C L-Arg + H2O
aldolase 3C L-citrulline + NH3
-
-
-
?
aldolase C L-Arg + H2O
aldolase C L-citrulline + NH3
-
-
-
?
alpha-enolase + H2O
[alpha-enolase]-L-citrulline + NH3
-
-
-
-
?
alpha-enolase 1 L-Arg + H2O
alpha-enolase 1 L-citrulline + NH3
-
-
-
?
benzoyl-Arg + H2O
benzoyl-citrulline + NH3
-
-
-
?
benzoyl-Arg ethyl ester + H2O
benzoyl-citrulline ethyl ester + NH3
benzoyl-Arg methyl ester + H2O
benzoyl-citrulline methyl ester + NH3
-
-
-
-
?
benzoyl-Arg-4-methylcoumarin-7-amide + H2O
benzoyl-citrulline-4-methylcoumarin-7-amide + NH3
-
-
-
-
?
benzoyl-Arg-ethyl ester + H2O
benzoyl-citrulline-ethyl ester + NH3
benzoyl-Arg-methyl ester + H2O
benzoyl-citrulline-methyl ester + NH3
benzoyl-Arg-NH2 + H2O
benzoyl-citrulline-NH2 + NH3
benzoyl-D-Arg-p-nitroanilide + H2O
benzoyl-citrulline-p-nitroanilide + NH3
-
18% of the rate of the L-isomer
-
-
?
benzoyl-Gly-L-Arg + H2O
benzoyl-Gly-L-citrulline + NH3
benzoyl-homoarginine ethyl ester + H2O
(2S)-2-aminobenzoyl-6-[(aminocarbonyl)amino]hexanoic acid + NH3
-
-
-
-
?
benzoyl-L-Arg + H2O
benzoyl-L-citrulline + NH3
benzoyl-L-Arg ethyl ester + H2O
benzoyl-L-citrulline ethyl ester + NH3
benzoyl-L-Arg-NH2 + H2O
benzoyl-L-citrullineamide + NH3
benzoyl-L-Arg-p-nitroanilide + H2O
benzoyl-citrulline-p-nitroanilide + NH3
-
-
-
-
?
benzoyl-L-arginine + H2O
benzoyl-L-citrulline + NH3
-
-
-
?
benzoyl-L-arginine amide + H2O
?
-
best small-molecule substrate for PAD4
-
-
?
benzoyl-L-arginine amide + H2O
benzoyl-L-citrulline amide + NH3
-
-
-
?
benzoyl-L-arginine ethyl ester + H2O
benzoyl-L-citrulline ethyl ester + NH3
benzoyl-L-arginine methyl ester + H2O
benzoyl-L-citrulline methyl ester + NH3
-
-
-
?
benzyloxycarbonyl-Arg-Arg-4-methylcoumarin-7-amide
? + NH3
-
-
-
-
?
benzyloxycarbonyl-Phe-Arg-4-methylcoumarin-7-amide + H2O
benzyloxycarbonyl-Phe-citrulline-4-methylcoumarin-7-amide + NH3
-
-
-
-
?
beta-actin L-arginine + H2O
beta-actin L-citrulline + NH3
substrate for isoform PAD2 only
-
-
?
beta2-tubulin L-Arg + H2O
beta2-tubulin L-citrulline + NH3
-
-
-
?
Boc-Gln-Arg-Arg-7-amido-4-methylcoumarin + H2O
? + NH3
bradykinin + H2O
9-citrulline bradykinin + NH3
-
-
-
-
?
CESSSHHPGIAEFPS-R + H2O
CESSSHHPGIAEFPS-citrulline + NH3
citrullinated histone H3 L-arginine + H2O
?
substrate for isoform PAD4 only
-
-
?
CRGKA + H2O
?
-
Giardia lamblia ADI specifically binds to and citrullinates the arginine in the conserved CRGKA tail of variant-specific surface proteins affecting both antigenic switching and antibody mediated cell death
-
-
?
CXC chemokine ligand 10 L-Arg + H2O
CXC chemokine ligand 10 citrulline + NH3
CXC chemokine ligand 11 L-arginine + H2O
CXC chemokine ligand 11 L-citrulline + NH3
CXCL17 L-Arg + H2O
CXCL17 L-citrulline + NH3
CXCL26 L-Arg + H2O
CXCL26 L-citrulline + NH3
CXCL5 L-Arg + H2O
CXCL5 L-citrulline + NH3
CXCL5-L-Arg + H2O
CXCL5-L-citrulline + NH3
-
PAD site-specifically deiminates L-Arg9 in CXCL5 to L-citrulline
-
-
?
CXCL8 L-Arg + H2O
CXCL8 L-citrulline + NH3
DDYSSSRDGYGGS + H2O
?
substrate for isoform PAD4
-
-
?
DGYGGSRDSYSSS + H2O
?
substrate for isoform PAD4
-
-
?
DSEGTWRKGPEAD + H2O
?
substrate for isoform PAD2
-
-
?
DSHKFDRDFIYSD + H2O
DSHKFDCitDFIYSD + NH3
DSHKHSREWLWSD + H2O
DSHKHSCitEWLWSD + NH3
DSHKWHRDFFYSD + H2O
DSHKWHCitDFFYSD + NH3
DSKFAFRGGIASD + H2O
DSKFAFCitGGIASD + NH3
DSKFHFRYAVASD + H2O
DSKFHFCitYAVASD + NH3
DSKFKFRYAYASD + H2O
DSKFKFCitYAYASD + NH3
DSKHFHRDFIYSD + H2O
DSKHFHCitDFIYSD + NH3
DSKHKSRDFVYSD + H2O
DSKHKSCitDFVYSD + NH3
DSKHLSREWMWSD + H2O
DSKHLSCitEWMWSD + NH3
DSKKFDRDHLYSD + H2O
DSKKFDCitDHLYSD + NH3
DSKKFDRGHLYSD + H2O
DSKKFDCitGHLYSD + NH3
DSKKFHRDFLYSD + H2O
DSKKFHCitDFLYSD + NH3
DSKKFHRGFLYSD + H2O
DSKKFHCitGFLYSD + NH3
DSKKFKRDFLFSD + H2O
DSKKFKCitDFLFSD + NH3
DSKKHDRDHLWSD + H2O
DSKKHDCitDHLWSD + NH3
DSKKHFRDKLYSD + H2O
DSKKHFCitDKLYSD + NH3
DSKKHFRGKLYSD + H2O
DSKKHFCitGKLYSD + NH3
DSKKKHREWVWSD + H2O
DSKKKHCitEWVWSD + NH3
DSKKLHRDHMESD + H2O
DSKKLHCitDHMESD + NH3
DSKKYDRDFLWSD + H2O
DSKKYDCitDFLWSD + NH3
DSKKYDRGFLWSD + H2O
DSKKYDCitGFLWSD + NH3
DSKWHHRDHLYSD + H2O
DSKWHHCitDHLYSD + NH3
DSKWHHRGHLYSD + H2O
DSKWHHCitGHLYSD + NH3
DSKWYHRNKFWSD + H2O
DSKWYHCitNKFWSD + NH3
DSQFAFRGASASD + H2O
DSQFAFCitGASASD + NH3
DSQWAFRHALFSD + H2O
DSQWAFCitHALFSD + NH3
DSRFYWRGGGKSD + H2O
?
substrate for isoform PAD2
-
-
?
DSSEELRGGGKSD + H2O
?
substrate for isoform PAD2
-
-
?
DSYRSWRDGYYSD + H2O
?
substrate for isoform PAD4
-
-
?
FFDSHKFDRDFIYSD + H2O
FFDSHKFDCitDFIYSD + NH3
FFDSHKHSREWLWSD + H2O
FFDSHKHSCitEWLWSD + NH3
FFDSHKKSREYVFSD + H2O
FFDSHKKSCitEYVFSD + NH3
FFDSHKLHREWMWSD + H2O
FFDSHKLHCitEWMWSD + NH3
FFDSHKWHRDFFYSD + H2O
FFDSHKWHCitDFFYSD + NH3
FFDSKHFDREWIWSD + H2O
FFDSKHFDCitEWIWSD + NH3
FFDSKHKSRDFVYSD + H2O
FFDSKHKSCitDFVYSD + NH3
FFDSKHLSREWMWSD + H2O
FFDSKHLSCitEWMWSD + NH3
FFDSKKFSRDHIESD + H2O
FFDSKKFSCitDHIESD + NH3
FFDSKKKHREWVWSD + H2O
FFDSKKKHCitEWVWSD + NH3
FFDSKKWSREYFFSD + H2O
FFDSKKWSCitEYFFSD + NH3
fibrin L-arginine + H2O
fibrin L-citrulline + NH3
fibrinogen + H2O
fibrinogen with citrullinated L-arginine residues
-
-
-
-
?
fibrinogen-L-arginine + H2O
fibrinogen-L-citrulline + NH3
filaggrin L-Arg + H2O
filaggrin citrulline + NH3
-
-
-
?
filaggrin L-Arg + H2O
filaggrin L-citrulline + NH3
-
-
-
-
?
filaggrin L-arginine + H2O
filaggrin L-citrulline + NH3
filaggrin-L-arginine + H2O
filaggrin-L-citrulline + NH3
gamma-actin L-arginine + H2O
gamma-actin L-citrulline + NH3
substrate for isoform PAD2 only
-
-
?
glial fibrillary acidic protein L-arginine + H2O
glial fibrillary acidic protein L-citrulline + NH3
-
-
-
?
glial fibrillary acidic protein-L-Arg + H2O
glial fibrillary acidic protein-L-citrulline + NH3
-
-
-
?
glial fibrillary acidic protein-L-arginine + H2O
glial fibrillary acidic protein-L-citrulline + NH3
Gly-Gly-L-Arg + H2O
Gly-Gly-L-Cit + NH3
Gly-L-Arg + H2O
Gly-citrulline + NH3
Gly-L-Arg + H2O
Gly-L-Cit + NH3
glycogen phosphorylase b + H2O
?
-
specific deimination by peptidylarginine deiminase has a pronounced effect on the binding of some allosteric effectors with the enzyme and hampers the association of phosphorylase b dimers into tetramers in the presence of AMP
-
-
?
glycogen phosphorylase b + H2O
? + NH3
-
specific deimination by peptidylarginine deiminase has a pronounced effect on the binding of some allosteric effectors with the enzyme and hampers the association of phosphorylase b dimers into tetramers in the presence of AMP
-
-
?
GYGGGSRDGSYGG + H2O
?
substrate for isoform PAD4
-
-
?
heat shock protein 8 L-Arg + H2O
heat shock protein 8 L-citrulline + NH3
-
-
-
?
histone H2A L-arginine + H2O
histone H2A L-citrulline + NH3
-
deimination occurs at Arg3 of the N-terminal sequence acetyl-SGRGK
-
-
?
histone H2A-L-arginine + H2O
histone H2A-L-citrulline + NH3
histone H3 L-Arg + H2O
histone H3 L-citrulline + NH3
-
citrullination activity is limited to diestrus with histone H3
-
-
?
histone H3 L-arginine + H2O
histone H3 L-citrulline + NH3
-
-
-
-
?
histone H3-L-Arg + H2O
histone H3-L-citrulline
histone H3-L-arginine + H2O
histone H3-L-citrulline + NH3
histone H4 L-arginine + H2O
histone H4 L-citrulline + NH3
-
deimination occurs at Arg3 of the N-terminal sequence acetyl-SGRGK
-
-
?
histone H4-L-arginine + H2O
histone H4-L-citrulline + NH3
histone L-arginine + H2O
histone L-citrulline + NH3
human vimentin L-arginine + H2O
human vimentin L-citrulline + NH3
-
-
-
-
?
IEIITDRQSGKKR + H2O
?
substrate for isoform PAD2
-
-
?
IEIMTDRGSGKKR + H2O
?
substrate for isoform PAD2
-
-
?
IGSRGDRSGFGKF + H2O
?
substrate for isoform PAD2
-
-
?
IHAREIFDSR + H2O
IHAREIFDS-citrulline + NH3
keratin L-arginine + H2O
keratin L-citrulline + NH3
keratin-L-arginine + H2O
keratin-L-citrulline + NH3
KKSIRDTPA + H2O
KKSI-citrulline-DTPA
-
-
-
?
KSIRDTP + H2O
KSI-citrulline-DTP
-
-
-
?
L-Arg + H2O
citrulline + NH3
-
-
-
?
L-Arg + H2O
L-citrulline + NH3
L-Arg methyl ester + H2O
L-citrulline methyl ester + NH4+
-
-
-
-
?
L-Arg-Gly + H2O
L-Cit-Gly + NH3
-
75% activity compared to Nalpha-benzoyl-L-arginine ethyl ester
-
-
?
L-Arg-Gly-Gly + H2O
L-Cit-Gly-Gly + NH3
-
66% activity compared to Nalpha-benzoyl-L-arginine ethyl ester
-
-
?
L-arginine + H2O
L-citrulline + NH3
L-arginine-methyl ester + H2O
L-citrulline methyl ester + NH3
L-canavanine + H2O
? + NH3
-
0.3% activity compared to L-arginine
-
-
?
L-homoarginine + H2O
? + NH3
-
0.3% activity compared to L-arginine
-
-
?
L-Leu-Trp-Met-Arg + H2O
L-Leu-Trp-Met-Cit + NH3
-
45% activity compared to Nalpha-benzoyl-L-arginine ethyl ester
-
-
?
L-Met-Arg-Phe + H2O
L-Met-Cit-Phe + NH3
-
the substrate shows fastest rate of citrullination with 158% activity compared to Nalpha-benzoyl-L-arginine ethyl ester
-
-
?
L-Met-L-Arg + H2O
L-Met-citrulline + NH3
L-octopine + H2O
? + NH3
-
16.7% activity compared to L-arginine
-
-
?
myelin basic protein + H2O
?
myelin basic protein L-arginine + H2O
myelin basic protein L-citrulline + NH3
myelin basic protein-L-Arg + H2O
myelin basic protein-L-citrulline + NH3
-
-
-
?
myelin basic protein-L-arginine + H2O
myelin basic protein-L-citrulline + NH3
N-(3-[1-[(2'-chloro-3'-fluoro[1,1'-biphenyl]-3-yl)methyl]-2,5-dioxoimidazolidin-4-yl]propyl)guanidine + H2O
N-(3-[1-[(2'-chloro-3'-fluoro[1,1'-biphenyl]-3-yl)methyl]-2,5-dioxoimidazolidin-4-yl]propyl)urea + NH3
-
-
-
?
N-(3-[1-[(2'-chloro[1,1'-biphenyl]-3-yl)methyl]-2,5-dioxoimidazolidin-4-yl]propyl)guanidine + H2O
N-(3-[1-[(2'-chloro[1,1'-biphenyl]-3-yl)methyl]-2,5-dioxoimidazolidin-4-yl]propyl)urea + NH3
-
-
-
?
N-(3-[1-[([1,1'-biphenyl]-3-yl)methyl]-2,5-dioxoimidazolidin-4-yl]propyl)urea + H2O
N-(3-[1-[(3'-fluoro[1,1'-biphenyl]-3-yl)methyl]-2,5-dioxoimidazolidin-4-yl]propyl)urea + NH3
-
-
-
?
N-(biphenyl-3-ylmethyl)-4-carbamimidamidobutanamide + H2O
N-[([1,1''-biphenyl]-3-yl)methyl]-4-(carbamoylamino)butanamide + NH3
-
-
-
?
N-acetyl-Arg methyl ester + H2O
N-acetyl-citrulline + NH4+
-
-
-
-
?
N-acetyl-L-arginine + H2O
N-acetyl-L-citrulline ethyl ester + NH3
N-acetyl-L-arginine-methyl ester + H2O
N-acetyl-L-citrulline-methyl ester + NH3
N-alpha-benzoyl-L-arginine + H2O
N-alpha-benzoyl-L-citrulline + NH3
N-alpha-benzoyl-L-arginine amide + H2O
N-alpha-benzoyl-L-citrulline amide + NH3
-
best small molecule substrate for isozyme PAD1
-
-
?
N-alpha-benzoyl-L-arginine ethyl ester + H2O
N-alpha-benzoyl-L-citrulline ethyl ester + NH3
N-alpha-benzoyl-L-arginine ethyl ester + H2O
N-alpha-benzoyl-L-citrulline methyl ester + NH3
-
-
-
?
N-alpha-benzoyl-L-arginine methyl ester + H2O
N-alpha-benzoyl-L-citrulline methyl ester + NH3
-
-
-
-
?
N-[(1R)-1-(biphenyl-3-yl)ethyl]-4-carbamimidamidobutanamide + H2O
N-[(1R)-1-([1,1''-biphenyl]-3-yl)ethyl]-4-(carbamoylamino)butanamide + NH3
-
-
-
?
N-[(1S)-1-(biphenyl-3-yl)ethyl]-4-carbamimidamidobutanamide + H2O
N-[(1S)-1-([1,1''-biphenyl]-3-yl)ethyl]-4-(carbamoylamino)butanamide + NH3
-
-
-
?
N-[(3'-bromobiphenyl-3-yl)methyl]-4-carbamimidamidobutanamide + H2O
N-[(3''-bromo[1,1''-biphenyl]-3-yl)methyl]-4-(carbamoylamino)butanamide + NH3
-
-
-
?
Nalpha-benzoyl L-arginine ethyl ester + H2O
Nalpha-benzoyl L-citrulline ethyl ester + NH3
-
-
-
-
?
Nalpha-benzoyl-L-arginine + H2O
Nalpha-benzoyl-L-citrulline + NH3
Nalpha-benzoyl-L-arginine amide + H2O
Nalpha-benzoyl-L-citrulline amide + NH3
Nalpha-benzoyl-L-arginine ethyl ester
Nalpha-benzoyl-L-citrulline ethyl ester + NH3
-
-
-
-
?
Nalpha-benzoyl-L-arginine ethyl ester + H2O
Nalpha-benzoyl-L-citrulline ethyl ester + NH3
Nalpha-benzoyl-L-arginine methyl ester + H2O
Nalpha-benzoyl-L-citrulline methyl ester + NH3
Nalpha-benzoyl-L-arginine-amide + H2O
Nalpha-benzoyl-L-citrulline-amide + NH3
Nalpha-tosyl-L-arginine-methyl ester + H2O
Nalpha-tosyl-L-citrulline-methyl ester + NH3
neuron-specific enolase 2 L-Arg + H2O
neuron-specific enolase 2 L-citrulline + NH3
-
-
-
?
neuron-specific-enolase1 + H2O
[neuron-specific]-enolase1 L-citrulline + NH3
-
-
-
-
?
p300-L-arginine + H2O
?
-
-
-
-
?
PAD4-L-arginine + H2O
PAD4-L-citrulline + NH3
-
PAD4 is autodeiminated in a time-dependent manner
-
-
?
peptide H3-1-L-arginine + H2O
peptide H3-1-L-citrulline + NH3
i.e. Ac4KQTARKSTGG13
-
-
?
peptide H3-2-L-arginine + H2O
peptide H3-2-L-citrulline + NH3
i.e. Ac14KAPRKQLATK23
-
-
?
peptide H4-L-arginine + H2O
peptide H4-L-citrulline + NH3
-
i.e. Ac1SGRGKGGKGL10
-
?
peptidylprolyl isomerase A L-Arg + H2O
peptidylprolyl isomerase A L-citrulline + NH3
-
-
-
?
peroxiredoxin 1 L-Arg + H2O
peroxiredoxin 1 L-citrulline + NH3
-
-
-
?
PPGFSPFR + H2O
PPGFSPF-citrulline + NH3
protamine + H2O
? + NH3
-
peptidyl arginine deiminase III shows highest activity towards
-
-
?
protein L-Arg + H2O
protein citrulline + NH3
-
-
-
-
?
protein L-Arg + H2O
protein L-citrulline + NH3
protein L-Arg + H2O
protein-L-citrulline + NH3
-
-
-
-
?
protein L-arginine + H2O
protein L-citrulline + NH3
protein-L-arginine + H2O
protein-L-citrulline + NH3
QLSLRTVSL + H2O
QLSL-citrulline-TVSL
-
-
-
?
S100A3-L-arginine + H2O
S100A3-L-citrulline + NH3
-
-
-
?
SHQESTRGKSKGKAAAAA + H2O
SHQEST-Cit-GKSKGKAAAAA + NH3
-
-
-
?
SIRDT + H2O
SI-citrulline-RDT
-
-
-
?
t-butyloxycarbonyl-Leu-Arg-Arg-4-methylcoumarin-7-amide + H2O
? + NH3
-
-
-
-
?
t-butyloxycarbonyl-Leu-Gly-Arg-4-methylcoumarin-7-amide + H2O
t-butyloxycarbonyl-Leu-Gly-citrulline-4-methylcoumarin-7-amide + NH3
-
-
-
-
?
t-butyloxycarbonyl-Leu-Lys-Arg-4-methylcoumarin-7-amide + H2O
t-butyloxycarbonyl-Leu-Lys-citrulline-4-methylcoumarin-7-amide + NH3
-
-
-
-
?
t-butyloxycarbonyl-Leu-Thr-Arg-4-methylcoumarin-7-amide + H2O
t-butyloxycarbonyl-Leu-Thr-citrulline-4-methylcoumarin-7-amide + NH3
-
-
-
-
?
t-butyloxycarbonyl-Val-Pro-Arg-4-methylcoumarin-7-amide + H2O
t-butyloxycarbonyl-Val-Pro-citrulline-4-methylcoumarin-7-amide + NH3
-
-
-
-
?
TKQTARKSTGGK + H2O
?
-
-
-
-
?
tosyl-Arg-ethyl ester + H2O
tosyl-citrulline-ethyl ester + NH3
-
hPADI2
-
-
?
tosyl-L-Arg ethyl ester + H2O
tosyl-L-citrulline ethyl ester + NH3
-
-
-
-
?
trichohyalin + H2O
?
-
-
-
-
?
trichohyalin + H2O
? + NH3
trichohyalin-L-arginine + H2O
trichohyalin-L-citrulline + NH3
TSTGGRQGSHH + H2O
?
-
-
-
-
?
variant-specific surface protein L-arginine + H2O
variant-specific surface protein L-citrulline + NH3
-
-
-
-
?
vimentin-L-arginine + H2O
vimentin-L-citrulline + NH3
vimetin L-arginine + H2O
vimentin L-citrulline + NH3
voltage-dependent anion channel 1 L-Arg + H2O
voltage-dependent anion channel 1 L-citrulline + NH3
-
-
-
?
yeast enolase L-arginine + H2O
yeast enolase L-citrulline + NH3
-
-
-
-
?
[cofilin 1]-L-Arg + H2O
[cofilin 1]-L-citrulline + NH3
-
-
-
?
[histone H4]-L-Arg + H2O
[histone H4]-L-citrulline + NH3
-
-
-
-
?
[IKKgamma]-L-arginine + H2O
[IKKgamma]-L-citrulline + NH3
-
-
-
-
?
[mitochondrial malate dehydrogenase 2]-L-arginine + H2O
[mitochondrial malate dehydrogenase 2]-L-citrulline + NH3
-
-
-
?
additional information
?
-
acetyl-L-Arg + H2O
acetyl-L-citrulline + NH3
peptidylarginine deiminase 1
-
-
?
acetyl-L-Arg + H2O
acetyl-L-citrulline + NH3
-
-
-
-
?
acetyl-L-Arg methyl ester + H2O
acetyl-L-citrulline methyl ester + NH3
peptidylarginine deiminase 1
-
-
?
acetyl-L-Arg methyl ester + H2O
acetyl-L-citrulline methyl ester + NH3
peptidylarginine deiminase 2
-
-
?
acetyl-L-Arg methyl ester + H2O
acetyl-L-citrulline methyl ester + NH3
peptidylarginine deiminase 3
-
-
?
benzoyl-Arg ethyl ester + H2O
benzoyl-citrulline ethyl ester + NH3
-
-
-
?
benzoyl-Arg ethyl ester + H2O
benzoyl-citrulline ethyl ester + NH3
-
-
-
-
?
benzoyl-Arg ethyl ester + H2O
benzoyl-citrulline ethyl ester + NH3
-
-
-
?
benzoyl-Arg ethyl ester + H2O
benzoyl-citrulline ethyl ester + NH3
-
peptidyl arginine deiminase II shows highest activity towards
-
-
?
benzoyl-Arg ethyl ester + H2O
benzoyl-citrulline ethyl ester + NH3
-
-
-
-
?
benzoyl-Arg ethyl ester + H2O
benzoyl-citrulline ethyl ester + NH3
-
-
-
-
?
benzoyl-Arg ethyl ester + H2O
benzoyl-citrulline ethyl ester + NH3
-
-
-
-
?
benzoyl-Arg-ethyl ester + H2O
benzoyl-citrulline-ethyl ester + NH3
-
hPADI2
-
-
?
benzoyl-Arg-ethyl ester + H2O
benzoyl-citrulline-ethyl ester + NH3
-
hPADI4
-
-
?
benzoyl-Arg-methyl ester + H2O
benzoyl-citrulline-methyl ester + NH3
-
hPADI2
-
-
?
benzoyl-Arg-methyl ester + H2O
benzoyl-citrulline-methyl ester + NH3
-
hPADI4
-
-
?
benzoyl-Arg-NH2 + H2O
benzoyl-citrulline-NH2 + NH3
-
hPADI2
-
-
?
benzoyl-Arg-NH2 + H2O
benzoyl-citrulline-NH2 + NH3
-
hPADI4
-
-
?
benzoyl-Gly-L-Arg + H2O
benzoyl-Gly-L-citrulline + NH3
-
-
-
-
?
benzoyl-Gly-L-Arg + H2O
benzoyl-Gly-L-citrulline + NH3
-
-
-
-
?
benzoyl-Gly-L-Arg + H2O
benzoyl-Gly-L-citrulline + NH3
-
-
-
-
?
benzoyl-Gly-L-Arg + H2O
benzoyl-Gly-L-citrulline + NH3
-
-
-
-
?
benzoyl-Gly-L-Arg + H2O
benzoyl-Gly-L-citrulline + NH3
-
-
-
-
ir
benzoyl-L-Arg + H2O
benzoyl-L-citrulline + NH3
-
-
-
-
?
benzoyl-L-Arg + H2O
benzoyl-L-citrulline + NH3
peptidylarginine deiminase 1
-
-
?
benzoyl-L-Arg + H2O
benzoyl-L-citrulline + NH3
peptidylarginine deiminase 2
-
-
?
benzoyl-L-Arg + H2O
benzoyl-L-citrulline + NH3
peptidylarginine deiminase 3
-
-
?
benzoyl-L-Arg + H2O
benzoyl-L-citrulline + NH3
-
-
-
-
?
benzoyl-L-Arg + H2O
benzoyl-L-citrulline + NH3
-
-
-
-
?
benzoyl-L-Arg + H2O
benzoyl-L-citrulline + NH3
-
-
-
-
?
benzoyl-L-Arg + H2O
benzoyl-L-citrulline + NH3
-
-
-
-
ir
benzoyl-L-Arg ethyl ester + H2O
benzoyl-L-citrulline ethyl ester + NH3
-
-
-
-
?
benzoyl-L-Arg ethyl ester + H2O
benzoyl-L-citrulline ethyl ester + NH3
peptidylarginine deiminase 1
-
-
?
benzoyl-L-Arg ethyl ester + H2O
benzoyl-L-citrulline ethyl ester + NH3
peptidylarginine deiminase 2
-
-
?
benzoyl-L-Arg ethyl ester + H2O
benzoyl-L-citrulline ethyl ester + NH3
peptidylarginine deiminase 3
-
-
?
benzoyl-L-Arg-NH2 + H2O
benzoyl-L-citrullineamide + NH3
-
-
-
-
?
benzoyl-L-Arg-NH2 + H2O
benzoyl-L-citrullineamide + NH3
-
peptidyl arginine deiminase I shows highest activity towards
-
-
?
benzoyl-L-Arg-NH2 + H2O
benzoyl-L-citrullineamide + NH3
-
-
-
-
?
benzoyl-L-Arg-NH2 + H2O
benzoyl-L-citrullineamide + NH3
-
-
-
-
?
benzoyl-L-Arg-NH2 + H2O
benzoyl-L-citrullineamide + NH3
-
-
-
-
?
benzoyl-L-Arg-NH2 + H2O
benzoyl-L-citrullineamide + NH3
-
-
-
-
ir
benzoyl-L-arginine ethyl ester + H2O
benzoyl-L-citrulline ethyl ester + NH3
-
-
-
?
benzoyl-L-arginine ethyl ester + H2O
benzoyl-L-citrulline ethyl ester + NH3
-
-
-
?
Boc-Gln-Arg-Arg-7-amido-4-methylcoumarin + H2O
? + NH3
-
-
-
-
?
Boc-Gln-Arg-Arg-7-amido-4-methylcoumarin + H2O
? + NH3
-
-
-
-
?
CESSSHHPGIAEFPS-R + H2O
CESSSHHPGIAEFPS-citrulline + NH3
i.e. fibrinogen peptide FibA-R
-
-
?
CESSSHHPGIAEFPS-R + H2O
CESSSHHPGIAEFPS-citrulline + NH3
i.e. fibrinogen peptide FibA-R
-
-
?
CXC chemokine ligand 10 L-Arg + H2O
CXC chemokine ligand 10 citrulline + NH3
-
-
-
-
?
CXC chemokine ligand 10 L-Arg + H2O
CXC chemokine ligand 10 citrulline + NH3
-
-
-
-
?
CXC chemokine ligand 11 L-arginine + H2O
CXC chemokine ligand 11 L-citrulline + NH3
-
-
-
-
?
CXC chemokine ligand 11 L-arginine + H2O
CXC chemokine ligand 11 L-citrulline + NH3
-
-
-
-
?
CXCL17 L-Arg + H2O
CXCL17 L-citrulline + NH3
-
-
-
?
CXCL17 L-Arg + H2O
CXCL17 L-citrulline + NH3
-
-
-
-
?
CXCL26 L-Arg + H2O
CXCL26 L-citrulline + NH3
-
-
-
?
CXCL26 L-Arg + H2O
CXCL26 L-citrulline + NH3
-
-
-
-
?
CXCL5 L-Arg + H2O
CXCL5 L-citrulline + NH3
-
-
-
?
CXCL5 L-Arg + H2O
CXCL5 L-citrulline + NH3
-
-
-
-
?
CXCL8 L-Arg + H2O
CXCL8 L-citrulline + NH3
citrullination of CXCL8 by peptidylarginine deiminase alters receptor usage, prevents proteolysis, and dampens tissue inflammation
-
-
?
CXCL8 L-Arg + H2O
CXCL8 L-citrulline + NH3
-
citrullination of CXCL8 by peptidylarginine deiminase alters receptor usage, prevents proteolysis, and dampens tissue inflammation
-
-
?
DSHKFDRDFIYSD + H2O
DSHKFDCitDFIYSD + NH3
79% conversion by isoform PAD4
-
-
?
DSHKFDRDFIYSD + H2O
DSHKFDCitDFIYSD + NH3
90% conversion by isoform PAD2
-
-
?
DSHKHSREWLWSD + H2O
DSHKHSCitEWLWSD + NH3
51% conversion by isoform PAD4
-
-
?
DSHKHSREWLWSD + H2O
DSHKHSCitEWLWSD + NH3
97% conversion by isoform PAD2
-
-
?
DSHKWHRDFFYSD + H2O
DSHKWHCitDFFYSD + NH3
73% conversion by isoform PAD4
-
-
?
DSHKWHRDFFYSD + H2O
DSHKWHCitDFFYSD + NH3
92% conversion by isoform PAD2
-
-
?
DSKFAFRGGIASD + H2O
DSKFAFCitGGIASD + NH3
25% conversion by isoform PAD4
-
-
?
DSKFAFRGGIASD + H2O
DSKFAFCitGGIASD + NH3
90% conversion by isoform PAD2
-
-
?
DSKFHFRYAVASD + H2O
DSKFHFCitYAVASD + NH3
92% conversion by isoform PAD2
-
-
?
DSKFHFRYAVASD + H2O
DSKFHFCitYAVASD + NH3
less than 20% conversion by isoform PAD4
-
-
?
DSKFKFRYAYASD + H2O
DSKFKFCitYAYASD + NH3
91% conversion by isoform PAD2
-
-
?
DSKFKFRYAYASD + H2O
DSKFKFCitYAYASD + NH3
less than 20% conversion by isoform PAD4
-
-
?
DSKHFHRDFIYSD + H2O
DSKHFHCitDFIYSD + NH3
69% conversion by isoform PAD4
-
-
?
DSKHFHRDFIYSD + H2O
DSKHFHCitDFIYSD + NH3
94% conversion by isoform PAD2
-
-
?
DSKHKSRDFVYSD + H2O
DSKHKSCitDFVYSD + NH3
69% conversion by isoform PAD4
-
-
?
DSKHKSRDFVYSD + H2O
DSKHKSCitDFVYSD + NH3
94% conversion by isoform PAD2
-
-
?
DSKHLSREWMWSD + H2O
DSKHLSCitEWMWSD + NH3
54% conversion by isoform PAD4
-
-
?
DSKHLSREWMWSD + H2O
DSKHLSCitEWMWSD + NH3
95% conversion by isoform PAD2
-
-
?
DSKKFDRDHLYSD + H2O
DSKKFDCitDHLYSD + NH3
77% conversion by isoform PAD4
-
-
?
DSKKFDRDHLYSD + H2O
DSKKFDCitDHLYSD + NH3
90% conversion by isoform PAD2
-
-
?
DSKKFDRGHLYSD + H2O
DSKKFDCitGHLYSD + NH3
80% conversion by isoform PAD4
-
-
?
DSKKFDRGHLYSD + H2O
DSKKFDCitGHLYSD + NH3
90% conversion by isoform PAD2
-
-
?
DSKKFHRDFLYSD + H2O
DSKKFHCitDFLYSD + NH3
64% conversion by isoform PAD4
-
-
?
DSKKFHRDFLYSD + H2O
DSKKFHCitDFLYSD + NH3
97% conversion by isoform PAD2
-
-
?
DSKKFHRGFLYSD + H2O
DSKKFHCitGFLYSD + NH3
100% conversion by isoform PAD2
-
-
?
DSKKFHRGFLYSD + H2O
DSKKFHCitGFLYSD + NH3
73% conversion by isoform PAD4
-
-
?
DSKKFKRDFLFSD + H2O
DSKKFKCitDFLFSD + NH3
35% conversion by isoform PAD4
-
-
?
DSKKFKRDFLFSD + H2O
DSKKFKCitDFLFSD + NH3
97% conversion by isoform PAD2
-
-
?
DSKKHDRDHLWSD + H2O
DSKKHDCitDHLWSD + NH3
80% conversion by isoform PAD4
-
-
?
DSKKHDRDHLWSD + H2O
DSKKHDCitDHLWSD + NH3
95% conversion by isoform PAD2
-
-
?
DSKKHFRDKLYSD + H2O
DSKKHFCitDKLYSD + NH3
37% conversion by isoform PAD4
-
-
?
DSKKHFRDKLYSD + H2O
DSKKHFCitDKLYSD + NH3
92% conversion by isoform PAD2
-
-
?
DSKKHFRGKLYSD + H2O
DSKKHFCitGKLYSD + NH3
22% conversion by isoform PAD4
-
-
?
DSKKHFRGKLYSD + H2O
DSKKHFCitGKLYSD + NH3
96% conversion by isoform PAD2
-
-
?
DSKKKHREWVWSD + H2O
DSKKKHCitEWVWSD + NH3
100% conversion by isoform PAD2
-
-
?
DSKKKHREWVWSD + H2O
DSKKKHCitEWVWSD + NH3
72% conversion by isoform PAD4
-
-
?
DSKKLHRDHMESD + H2O
DSKKLHCitDHMESD + NH3
65% conversion by isoform PAD4
-
-
?
DSKKLHRDHMESD + H2O
DSKKLHCitDHMESD + NH3
90% conversion by isoform PAD2
-
-
?
DSKKYDRDFLWSD + H2O
DSKKYDCitDFLWSD + NH3
76% conversion by isoform PAD4
-
-
?
DSKKYDRDFLWSD + H2O
DSKKYDCitDFLWSD + NH3
92% conversion by isoform PAD2
-
-
?
DSKKYDRGFLWSD + H2O
DSKKYDCitGFLWSD + NH3
81% conversion by isoform PAD4
-
-
?
DSKKYDRGFLWSD + H2O
DSKKYDCitGFLWSD + NH3
93% conversion by isoform PAD2
-
-
?
DSKWHHRDHLYSD + H2O
DSKWHHCitDHLYSD + NH3
71% conversion by isoform PAD4
-
-
?
DSKWHHRDHLYSD + H2O
DSKWHHCitDHLYSD + NH3
90% conversion by isoform PAD2
-
-
?
DSKWHHRGHLYSD + H2O
DSKWHHCitGHLYSD + NH3
71% conversion by isoform PAD4
-
-
?
DSKWHHRGHLYSD + H2O
DSKWHHCitGHLYSD + NH3
96% conversion by isoform PAD2
-
-
?
DSKWYHRNKFWSD + H2O
DSKWYHCitNKFWSD + NH3
45% conversion by isoform PAD4
-
-
?
DSKWYHRNKFWSD + H2O
DSKWYHCitNKFWSD + NH3
98% conversion by isoform PAD2
-
-
?
DSQFAFRGASASD + H2O
DSQFAFCitGASASD + NH3
less than 20% conversion by isoform PAD2
-
-
?
DSQFAFRGASASD + H2O
DSQFAFCitGASASD + NH3
less than 20% conversion by isoform PAD4
-
-
?
DSQWAFRHALFSD + H2O
DSQWAFCitHALFSD + NH3
95% conversion by isoform PAD2
-
-
?
DSQWAFRHALFSD + H2O
DSQWAFCitHALFSD + NH3
less than 20% conversion by isoform PAD4
-
-
?
FFDSHKFDRDFIYSD + H2O
FFDSHKFDCitDFIYSD + NH3
94% conversion by isoform PAD4
-
-
?
FFDSHKFDRDFIYSD + H2O
FFDSHKFDCitDFIYSD + NH3
97% conversion by isoform PAD2
-
-
?
FFDSHKHSREWLWSD + H2O
FFDSHKHSCitEWLWSD + NH3
22% conversion by isoform PAD4
-
-
?
FFDSHKHSREWLWSD + H2O
FFDSHKHSCitEWLWSD + NH3
96% conversion by isoform PAD2
-
-
?
FFDSHKKSREYVFSD + H2O
FFDSHKKSCitEYVFSD + NH3
100% conversion by isoform PAD2
-
-
?
FFDSHKKSREYVFSD + H2O
FFDSHKKSCitEYVFSD + NH3
58% conversion by isoform PAD4
-
-
?
FFDSHKLHREWMWSD + H2O
FFDSHKLHCitEWMWSD + NH3
91% conversion by isoform PAD4
-
-
?
FFDSHKLHREWMWSD + H2O
FFDSHKLHCitEWMWSD + NH3
92% conversion by isoform PAD2
-
-
?
FFDSHKWHRDFFYSD + H2O
FFDSHKWHCitDFFYSD + NH3
88% conversion by isoform PAD4
-
-
?
FFDSHKWHRDFFYSD + H2O
FFDSHKWHCitDFFYSD + NH3
9% conversion by isoform PAD2
-
-
?
FFDSKHFDREWIWSD + H2O
FFDSKHFDCitEWIWSD + NH3
89% conversion by isoform PAD4
-
-
?
FFDSKHFDREWIWSD + H2O
FFDSKHFDCitEWIWSD + NH3
94% conversion by isoform PAD2
-
-
?
FFDSKHKSRDFVYSD + H2O
FFDSKHKSCitDFVYSD + NH3
77% conversion by isoform PAD4
-
-
?
FFDSKHKSRDFVYSD + H2O
FFDSKHKSCitDFVYSD + NH3
92% conversion by isoform PAD2
-
-
?
FFDSKHLSREWMWSD + H2O
FFDSKHLSCitEWMWSD + NH3
73% conversion by isoform PAD4
-
-
?
FFDSKHLSREWMWSD + H2O
FFDSKHLSCitEWMWSD + NH3
97% conversion by isoform PAD2
-
-
?
FFDSKKFSRDHIESD + H2O
FFDSKKFSCitDHIESD + NH3
63% conversion by isoform PAD4
-
-
?
FFDSKKFSRDHIESD + H2O
FFDSKKFSCitDHIESD + NH3
90% conversion by isoform PAD2
-
-
?
FFDSKKKHREWVWSD + H2O
FFDSKKKHCitEWVWSD + NH3
76% conversion by isoform PAD4
-
-
?
FFDSKKKHREWVWSD + H2O
FFDSKKKHCitEWVWSD + NH3
99% conversion by isoform PAD2
-
-
?
FFDSKKWSREYFFSD + H2O
FFDSKKWSCitEYFFSD + NH3
76% conversion by isoform PAD4
-
-
?
FFDSKKWSREYFFSD + H2O
FFDSKKWSCitEYFFSD + NH3
94% conversion by isoform PAD2
-
-
?
fibrin L-arginine + H2O
fibrin L-citrulline + NH3
-
-
-
-
?
fibrin L-arginine + H2O
fibrin L-citrulline + NH3
-
citrullinated fibrin is a potential antigen of rheumatoid arthritis
-
-
?
fibrin L-arginine + H2O
fibrin L-citrulline + NH3
-
-
-
-
?
fibrin L-arginine + H2O
fibrin L-citrulline + NH3
-
-
-
-
?
Fibrinogen + H2O
?
-
human fibrinogen, hPADI2
-
-
?
Fibrinogen + H2O
?
-
human fibrinogen, hPADI4
-
-
?
fibrinogen-L-arginine + H2O
fibrinogen-L-citrulline + NH3
PAD4, involvement in rheumatoid arthritis
-
-
ir
fibrinogen-L-arginine + H2O
fibrinogen-L-citrulline + NH3
PAD4
-
-
ir
filaggrin + H2O
?
-
human filaggrin, hPADI2
-
-
?
filaggrin + H2O
?
-
human filaggrin, hPADI4
-
-
?
filaggrin + H2O
? + NH3
-
deimination of protein-bound Arg residues to citrulline, the substrate has little structural order, the reaction proceeds rapidly to about 95% completion und results in loss of the organized structure
-
-
?
filaggrin + H2O
? + NH3
-
-
-
-
?
filaggrin L-arginine + H2O
filaggrin L-citrulline + NH3
isoform PAD1
-
-
?
filaggrin L-arginine + H2O
filaggrin L-citrulline + NH3
isoform PAD3
-
-
?
filaggrin L-arginine + H2O
filaggrin L-citrulline + NH3
peptidylarginine deiminase 1
-
-
?
filaggrin L-arginine + H2O
filaggrin L-citrulline + NH3
peptidylarginine deiminase 2
-
-
?
filaggrin L-arginine + H2O
filaggrin L-citrulline + NH3
peptidylarginine deiminase 3
-
-
?
filaggrin-L-arginine + H2O
filaggrin-L-citrulline + NH3
PAD4, involvement in rheumatoid arthritis
-
-
ir
filaggrin-L-arginine + H2O
filaggrin-L-citrulline + NH3
PAD4
-
-
ir
glial fibrillary acidic protein-L-arginine + H2O
glial fibrillary acidic protein-L-citrulline + NH3
PAD2, involvement in Alzheimer's diease
-
-
ir
glial fibrillary acidic protein-L-arginine + H2O
glial fibrillary acidic protein-L-citrulline + NH3
PAD2
-
-
ir
Gly-Gly-L-Arg + H2O
Gly-Gly-L-Cit + NH3
-
88% activity compared to Nalpha-benzoyl-L-arginine ethyl ester
-
-
?
Gly-Gly-L-Arg + H2O
Gly-Gly-L-Cit + NH3
-
88% activity compared to Nalpha-benzoyl-L-arginine ethyl ester
-
-
?
Gly-L-Arg + H2O
Gly-citrulline + NH3
-
-
-
?
Gly-L-Arg + H2O
Gly-citrulline + NH3
-
-
-
?
Gly-L-Arg + H2O
Gly-L-Cit + NH3
-
130% activity compared to Nalpha-benzoyl-L-arginine ethyl ester
-
-
?
Gly-L-Arg + H2O
Gly-L-Cit + NH3
-
130% activity compared to Nalpha-benzoyl-L-arginine ethyl ester
-
-
?
histone H2A-L-arginine + H2O
histone H2A-L-citrulline + NH3
-
-
-
-
?
histone H2A-L-arginine + H2O
histone H2A-L-citrulline + NH3
PAD4, involvement in rheumatoid arthritis
-
-
ir
histone H2A-L-arginine + H2O
histone H2A-L-citrulline + NH3
PAD4
-
-
ir
histone H3-L-Arg + H2O
histone H3-L-citrulline
-
PADI4 is a histone H3-specific arginine deiminase. Deimination is a mechanism for antagonizing the transcriptional induction mediated by arginine methylation
-
-
?
histone H3-L-Arg + H2O
histone H3-L-citrulline
-
PADI4 is a histone H3-specific arginine deiminase
-
-
?
histone H3-L-arginine + H2O
histone H3-L-citrulline + NH3
-
-
-
-
?
histone H3-L-arginine + H2O
histone H3-L-citrulline + NH3
-
-
-
?
histone H3-L-arginine + H2O
histone H3-L-citrulline + NH3
PAD4, involvement in rheumatoid arthritis
-
-
ir
histone H3-L-arginine + H2O
histone H3-L-citrulline + NH3
PAD4
-
-
ir
histone H3-L-arginine + H2O
histone H3-L-citrulline + NH3
PAD4 interacts with p53 and is recruited to the p21 promoter to regulate histone H3 L-Arg methylation and citrullination
-
-
?
histone H4-L-arginine + H2O
histone H4-L-citrulline + NH3
-
-
-
-
?
histone H4-L-arginine + H2O
histone H4-L-citrulline + NH3
PAD4, involvement in rheumatoid arthritis
-
-
ir
histone H4-L-arginine + H2O
histone H4-L-citrulline + NH3
PAD4
-
-
ir
histone H4-L-arginine + H2O
histone H4-L-citrulline + NH3
-
isozyme PAD4 deiminates histone H4 at arginines 3, 17, and 19 in vitro and arginine 3 in vivo
-
-
?
histone L-arginine + H2O
histone L-citrulline + NH3
-
-
-
-
?
histone L-arginine + H2O
histone L-citrulline + NH3
-
PAD4 mediates gene expression by regulating Arg methylation and citrullination in histones
-
-
?
IHAREIFDSR + H2O
IHAREIFDS-citrulline + NH3
peptide derived from enolase
-
-
?
IHAREIFDSR + H2O
IHAREIFDS-citrulline + NH3
-
peptide derived from enolase
-
-
?
IHAREIFDSR + H2O
IHAREIFDS-citrulline + NH3
peptide derived from enolase
-
-
?
keratin + H2O
? + NH3
-
-
-
-
?
keratin + H2O
? + NH3
-
-
-
-
?
keratin + H2O
? + NH3
-
-
-
-
?
keratin + H2O
? + NH3
-
peptidylarginine deiminase type I acts on the arginine residue in the V2 subdomain of mouse keratin more readily than peptidylarginine deiminase type II
-
-
?
keratin L-arginine + H2O
keratin L-citrulline + NH3
peptidylarginine deiminase 1
-
-
?
keratin L-arginine + H2O
keratin L-citrulline + NH3
peptidylarginine deiminase 2
-
-
?
keratin L-arginine + H2O
keratin L-citrulline + NH3
peptidylarginine deiminase 3
-
-
?
keratin-L-arginine + H2O
keratin-L-citrulline + NH3
PAD1
-
-
ir
keratin-L-arginine + H2O
keratin-L-citrulline + NH3
PAD2, involvement in psoriasis
-
-
ir
keratin-L-arginine + H2O
keratin-L-citrulline + NH3
PAD2
-
-
ir
L-Arg + H2O
L-citrulline + NH3
-
-
-
-
?
L-Arg + H2O
L-citrulline + NH3
-
-
-
-
?
L-Arg + H2O
L-citrulline + NH3
-
negligible action
-
-
?
L-arginine + H2O
L-citrulline + NH3
-
28% activity compared to Nalpha-benzoyl-L-arginine ethyl ester
-
-
?
L-arginine + H2O
L-citrulline + NH3
-
28% activity compared to Nalpha-benzoyl-L-arginine ethyl ester
-
-
?
L-arginine-methyl ester + H2O
L-citrulline methyl ester + NH3
isoform PAD1 shows 15% activity compared to Nalpha-benzoyl-L-arginine ethyl ester
-
-
?
L-arginine-methyl ester + H2O
L-citrulline methyl ester + NH3
isoform PAD3 shows 15% activity compared to Nalpha-benzoyl-L-arginine ethyl ester
-
-
?
L-Met-L-Arg + H2O
L-Met-citrulline + NH3
-
-
-
?
L-Met-L-Arg + H2O
L-Met-citrulline + NH3
-
-
-
?
myelin basic protein + H2O
?
-
-
-
-
?
myelin basic protein + H2O
?
-
decreased methylation of myelin basic protein possibly by a shortage of S-adenosylmethionine or downregulation of the methyltransferase gen expression allows the peptidylarginine deiminase greater access to the arginyl groups of myelin basic protein
-
-
?
myelin basic protein L-arginine + H2O
myelin basic protein L-citrulline + NH3
-
-
-
-
?
myelin basic protein L-arginine + H2O
myelin basic protein L-citrulline + NH3
-
-
-
?
myelin basic protein L-arginine + H2O
myelin basic protein L-citrulline + NH3
PAD2 overexpression in brain of transgenic mice leads to myelin loss in the central nervous system
-
-
?
myelin basic protein L-arginine + H2O
myelin basic protein L-citrulline + NH3
-
-
-
?
myelin basic protein-L-arginine + H2O
myelin basic protein-L-citrulline + NH3
-
PAD2 and PAD4 are involved in the pathogenesis of demyelination diseases such as multiple sclerosis, overview
-
-
?
myelin basic protein-L-arginine + H2O
myelin basic protein-L-citrulline + NH3
PAD2, involvement in multiple sclerosis
-
-
ir
myelin basic protein-L-arginine + H2O
myelin basic protein-L-citrulline + NH3
-
amino acid sequence determination of the substrate protein by LC/MS, PAD2 deiminates 18 of 19 arginyl residues, whereas PAD4 deiminates 14 of 19 arginyl residues, determination of deimination residues, overview
-
-
?
myelin basic protein-L-arginine + H2O
myelin basic protein-L-citrulline + NH3
PAD2
-
-
ir
myelin basic protein-L-arginine + H2O
myelin basic protein-L-citrulline + NH3
-
-
-
?
myelin basic protein-L-arginine + H2O
myelin basic protein-L-citrulline + NH3
-
-
-
?
N-acetyl-L-arginine + H2O
N-acetyl-L-citrulline ethyl ester + NH3
isoform PAD1 shows 160% activity compared to Nalpha-benzoyl-L-arginine ethyl ester
-
-
?
N-acetyl-L-arginine + H2O
N-acetyl-L-citrulline ethyl ester + NH3
isoform PAD3 shows 135% activity compared to Nalpha-benzoyl-L-arginine ethyl ester
-
-
?
N-acetyl-L-arginine-methyl ester + H2O
N-acetyl-L-citrulline-methyl ester + NH3
isoform PAD1 shows 80% activity compared to Nalpha-benzoyl-L-arginine ethyl ester
-
-
?
N-acetyl-L-arginine-methyl ester + H2O
N-acetyl-L-citrulline-methyl ester + NH3
isoform PAD3 shows 125% activity compared to Nalpha-benzoyl-L-arginine ethyl ester
-
-
?
N-alpha-benzoyl-L-arginine + H2O
N-alpha-benzoyl-L-citrulline + NH3
-
-
-
-
?
N-alpha-benzoyl-L-arginine + H2O
N-alpha-benzoyl-L-citrulline + NH3
-
-
-
-
?
N-alpha-benzoyl-L-arginine ethyl ester + H2O
N-alpha-benzoyl-L-citrulline ethyl ester + NH3
-
-
-
-
?
N-alpha-benzoyl-L-arginine ethyl ester + H2O
N-alpha-benzoyl-L-citrulline ethyl ester + NH3
-
-
-
?
N-alpha-benzoyl-L-arginine ethyl ester + H2O
N-alpha-benzoyl-L-citrulline ethyl ester + NH3
-
-
-
?
N-alpha-benzoyl-L-arginine ethyl ester + H2O
N-alpha-benzoyl-L-citrulline ethyl ester + NH3
-
-
-
-
?
N-alpha-benzoyl-L-arginine ethyl ester + H2O
N-alpha-benzoyl-L-citrulline ethyl ester + NH3
-
-
-
-
?
N-alpha-benzoyl-L-arginine ethyl ester + H2O
N-alpha-benzoyl-L-citrulline ethyl ester + NH3
-
-
-
-
?
N-alpha-benzoyl-L-arginine ethyl ester + H2O
N-alpha-benzoyl-L-citrulline ethyl ester + NH3
-
-
-
-
?
N-alpha-benzoyl-L-arginine ethyl ester + H2O
N-alpha-benzoyl-L-citrulline ethyl ester + NH3
-
-
-
?
N-alpha-benzoyl-L-arginine ethyl ester + H2O
N-alpha-benzoyl-L-citrulline ethyl ester + NH3
-
-
-
?
N-alpha-benzoyl-L-arginine ethyl ester + H2O
N-alpha-benzoyl-L-citrulline ethyl ester + NH3
-
-
-
-
?
Nalpha-benzoyl-L-arginine + H2O
Nalpha-benzoyl-L-citrulline + NH3
isoform PAD1 shows 120% activity compared to Nalpha-benzoyl-L-arginine ethyl ester
-
-
?
Nalpha-benzoyl-L-arginine + H2O
Nalpha-benzoyl-L-citrulline + NH3
isoform PAD3 shows 70% activity compared to Nalpha-benzoyl-L-arginine ethyl ester
-
-
?
Nalpha-benzoyl-L-arginine + H2O
Nalpha-benzoyl-L-citrulline + NH3
-
-
-
?
Nalpha-benzoyl-L-arginine + H2O
Nalpha-benzoyl-L-citrulline + NH3
-
-
-
?
Nalpha-benzoyl-L-arginine amide + H2O
Nalpha-benzoyl-L-citrulline amide + NH3
-
-
-
?
Nalpha-benzoyl-L-arginine amide + H2O
Nalpha-benzoyl-L-citrulline amide + NH3
-
-
-
?
Nalpha-benzoyl-L-arginine ethyl ester + H2O
Nalpha-benzoyl-L-citrulline ethyl ester + NH3
-
-
-
-
?
Nalpha-benzoyl-L-arginine ethyl ester + H2O
Nalpha-benzoyl-L-citrulline ethyl ester + NH3
100% activity
-
-
?
Nalpha-benzoyl-L-arginine ethyl ester + H2O
Nalpha-benzoyl-L-citrulline ethyl ester + NH3
-
-
-
?
Nalpha-benzoyl-L-arginine ethyl ester + H2O
Nalpha-benzoyl-L-citrulline ethyl ester + NH3
100% activity
-
-
?
Nalpha-benzoyl-L-arginine ethyl ester + H2O
Nalpha-benzoyl-L-citrulline ethyl ester + NH3
100% activity
-
-
?
Nalpha-benzoyl-L-arginine ethyl ester + H2O
Nalpha-benzoyl-L-citrulline ethyl ester + NH3
-
-
-
-
?
Nalpha-benzoyl-L-arginine ethyl ester + H2O
Nalpha-benzoyl-L-citrulline ethyl ester + NH3
-
100% activity
-
-
?
Nalpha-benzoyl-L-arginine ethyl ester + H2O
Nalpha-benzoyl-L-citrulline ethyl ester + NH3
-
100% activity
-
-
?
Nalpha-benzoyl-L-arginine methyl ester + H2O
Nalpha-benzoyl-L-citrulline methyl ester + NH3
-
-
-
?
Nalpha-benzoyl-L-arginine methyl ester + H2O
Nalpha-benzoyl-L-citrulline methyl ester + NH3
-
-
-
?
Nalpha-benzoyl-L-arginine-amide + H2O
Nalpha-benzoyl-L-citrulline-amide + NH3
isoform PAD1 shows 110% activity compared to Nalpha-benzoyl-L-arginine ethyl ester
-
-
?
Nalpha-benzoyl-L-arginine-amide + H2O
Nalpha-benzoyl-L-citrulline-amide + NH3
isoform PAD3 shows 105% activity compared to Nalpha-benzoyl-L-arginine ethyl ester
-
-
?
Nalpha-tosyl-L-arginine-methyl ester + H2O
Nalpha-tosyl-L-citrulline-methyl ester + NH3
isoform PAD1 shows 20% activity compared to Nalpha-benzoyl-L-arginine ethyl ester
-
-
?
Nalpha-tosyl-L-arginine-methyl ester + H2O
Nalpha-tosyl-L-citrulline-methyl ester + NH3
isoform PAD3 shows 22% activity compared to Nalpha-benzoyl-L-arginine ethyl ester
-
-
?
Polyarginine + H2O
?
-
-
-
-
?
Polyarginine + H2O
?
-
-
-
-
?
Polyarginine + H2O
?
-
-
-
-
?
PPGFSPFR + H2O
PPGFSPF-citrulline + NH3
peptide derived from bradykinin
-
-
?
PPGFSPFR + H2O
PPGFSPF-citrulline + NH3
-
peptide derived from bradykinin
-
-
?
PPGFSPFR + H2O
PPGFSPF-citrulline + NH3
peptide derived from bradykinin
-
-
?
protein L-Arg + H2O
?
-
epidermal cell membrane proteins
-
-
?
protein L-Arg + H2O
?
-
C-1, the most unmodified of the components of myelin basic protein, is an excellent substrate for the enzyme, thereby confirming that the origin of the citrulline in myelin basic protein arises by the action of this enzyme
-
-
?
protein L-Arg + H2O
?
-
protein-modulating enzyme
-
-
?
protein L-Arg + H2O
?
-
expression of the enzyme in luminal and glandular epithelia of the uterus is controlled by amount of the steroid hormones estrogen and progesterone
-
-
?
protein L-Arg + H2O
?
-
-
-
-
?
protein L-Arg + H2O
?
-
the ureido group on the citrulline formed by the peptidylarginine deiminase modification functions to unfold proteins due to decrease in net charge, loss of potential ionic bonds, and interference with H bonds
-
-
?
protein L-Arg + H2O
?
-
peptidylarginine deiminase, acting in concert with arginine-specific proteinases from Porphyromonas gingivalis, promotes the growth of the pathogen in the periodontal pocket, initially by enhancing its survivability and then by assisting the organism in its circumvention of host humoral defenses
-
-
?
protein L-Arg + H2O
?
-
post-translational modification enzyme
-
-
?
protein L-Arg + H2O
?
-
all-trans retinoic acid increases peptidylarginine deiminase activity in a newborn rat keratinocyte cell line
-
-
?
protein L-Arg + H2O
?
-
epidermal cell membrane proteins
-
-
?
protein L-Arg + H2O
?
-
citrullyl residues in the membranous protein of horny cells of the epidermis of newborn rat are formed by the action of epidermal peptidylarginine deiminase
-
-
?
protein L-Arg + H2O
protein L-citrulline + NH3
-
human myelin basic protein
-
-
?
protein L-Arg + H2O
protein L-citrulline + NH3
-
no activity with histones
-
-
?
protein L-Arg + H2O
protein L-citrulline + NH3
-
bovine S-carboxymethylated prekeratin and myelin
-
-
?
protein L-Arg + H2O
protein L-citrulline + NH3
-
histone
-
-
?
protein L-Arg + H2O
protein L-citrulline + NH3
-
histone
-
-
?
protein L-Arg + H2O
protein L-citrulline + NH3
peptidyl deiminase type II is the primary enzyme responsible for the conversion of protein bound arginine to citrulline in the central nervous system
-
-
?
protein L-Arg + H2O
protein L-citrulline + NH3
-
-
-
-
?
protein L-Arg + H2O
protein L-citrulline + NH3
-
-
-
-
?
protein L-Arg + H2O
protein L-citrulline + NH3
-
[NV]-angiotensin II, fibronectin fragment, LHRH, chicken brain peptide, molluscan cardioexxitatory neuropeptide, bradykinin potentiator B, dynorphin A, [VA]-kemptide, neurotensin, bradykinin, K-bradykinin, MK-bradykinin, Y-bradykinin
-
-
?
protein L-Arg + H2O
protein L-citrulline + NH3
-
bradykinin
-
-
?
protein L-Arg + H2O
protein L-citrulline + NH3
-
PAD catalyzes the deimination of the guanidino group from peptidylarginine residues of various peptides to produce peptidylcitrulline and ammonia
-
-
?
protein L-Arg + H2O
protein L-citrulline + NH3
-
histone
-
-
?
protein L-arginine + H2O
protein L-citrulline + NH3
-
-
-
?
protein L-arginine + H2O
protein L-citrulline + NH3
-
-
-
?
protein L-arginine + H2O
protein L-citrulline + NH3
-
-
-
?
protein L-arginine + H2O
protein L-citrulline + NH3
-
-
-
?
protein L-arginine + H2O
protein L-citrulline + NH3
-
-
-
?
protein L-arginine + H2O
protein L-citrulline + NH3
-
-
-
?
protein L-arginine + H2O
protein L-citrulline + NH3
-
-
-
?
protein L-arginine + H2O
protein L-citrulline + NH3
-
-
-
?
protein L-arginine + H2O
protein L-citrulline + NH3
-
-
-
?
protein-L-arginine + H2O
protein-L-citrulline + NH3
-
-
-
-
?
protein-L-arginine + H2O
protein-L-citrulline + NH3
-
-
-
?
protein-L-arginine + H2O
protein-L-citrulline + NH3
-
-
-
ir
protein-L-arginine + H2O
protein-L-citrulline + NH3
-
-
-
?
protein-L-arginine + H2O
protein-L-citrulline + NH3
-
-
-
?
protein-L-arginine + H2O
protein-L-citrulline + NH3
citrullination of proteins may exert a specific role in the course of PNS development and repair
-
-
?
protein-L-arginine + H2O
protein-L-citrulline + NH3
PAD1
-
-
ir
protein-L-arginine + H2O
protein-L-citrulline + NH3
PAD4 is a transcriptional coregulator that catalyzes the calcium-dependent conversion of specific arginine residues in proteins to citrulline and plays a role in the onset and progression of the chronic autoimmune disorder rheumatoid arthritis
-
-
?
protein-L-arginine + H2O
protein-L-citrulline + NH3
-
posttranslational convertion, PADI4 plays an essential role in immune cell differentiation and apoptosis, ionomycin-inducible PADI4-decreased cell viability and PADI4-enhanced apoptosis, overview
-
-
?
protein-L-arginine + H2O
protein-L-citrulline + NH3
development and evaluation of a highly sensitive antibody-based assay method for PAD activity determination, overview
-
-
?
protein-L-arginine + H2O
protein-L-citrulline + NH3
-
-
-
-
?
protein-L-arginine + H2O
protein-L-citrulline + NH3
-
development and evaluation of a highly sensitive antibody-based assay method for PAD activity determination, overview
-
-
?
protein-L-arginine + H2O
protein-L-citrulline + NH3
-
-
-
-
?
protein-L-arginine + H2O
protein-L-citrulline + NH3
-
development and evaluation of a highly sensitive antibody-based assay method for PAD activity determination, overview
-
-
?
protein-L-arginine + H2O
protein-L-citrulline + NH3
-
-
-
-
?
protein-L-arginine + H2O
protein-L-citrulline + NH3
-
development and evaluation of a highly sensitive antibody-based assay method for PAD activity determination, overview
-
-
?
protein-L-arginine + H2O
protein-L-citrulline + NH3
-
-
-
-
?
protein-L-arginine + H2O
protein-L-citrulline + NH3
-
-
-
ir
protein-L-arginine + H2O
protein-L-citrulline + NH3
-
citrullination of proteins may exert a specific role in the course of PNS development and repair
-
-
?
trichohyalin + H2O
? + NH3
-
-
-
-
?
trichohyalin + H2O
? + NH3
-
-
-
-
?
trichohyalin + H2O
? + NH3
-
deimination of protein-bound Arg residues to citrulline, the substrate has a highly alpha-helical structure, the reaction proceeds slowly to about 25% and can be forced to a maximum of about 65%
-
-
?
trichohyalin + H2O
? + NH3
-
-
-
-
?
trichohyalin-L-arginine + H2O
trichohyalin-L-citrulline + NH3
PAD3, involvement in skin disease
-
-
ir
trichohyalin-L-arginine + H2O
trichohyalin-L-citrulline + NH3
PAD3
-
-
ir
vimentin-L-arginine + H2O
vimentin-L-citrulline + NH3
-
-
-
-
?
vimentin-L-arginine + H2O
vimentin-L-citrulline + NH3
PAD2, involvement in Alzheimer's disease
-
-
ir
vimentin-L-arginine + H2O
vimentin-L-citrulline + NH3
PAD2
-
-
ir
vimetin L-arginine + H2O
vimentin L-citrulline + NH3
peptidylarginine deiminase 2
-
-
?
vimetin L-arginine + H2O
vimentin L-citrulline + NH3
peptidylarginine deiminase 4
-
-
?
additional information
?
-
-
PAD II not only binds with the beta-amyloid peptides Abeta1-40, Abeta22-35, Abeta17-28,Abeta25-35 and Abeta32-35 but assists in the proteolytic degradation of these peptides with the concomitant formation of insoluble fibrils
-
-
?
additional information
?
-
no activity with L-arginine
-
-
?
additional information
?
-
no activity with L-arginine
-
-
?
additional information
?
-
no activity with L-arginine
-
-
?
additional information
?
-
-
no activity with L-arginine
-
-
?
additional information
?
-
-
abnormal accumulation of citrullinated proteins and abnormal activation of PAD2 in hippocampi of patients with Alzheimer's disease, strongly suggests that peptidylarginine deiminase has an important role in the onset and progression of abnormal accumulation of citrullinated proteins and abnormal activation of PAD2 in hippocampi of Alzheimer's disease
-
-
?
additional information
?
-
peptidylarginine deiminase type 4 plays an essential role in pathogenesis of rheumatoid arthritis.Peptidylarginine deiminase type 4 may contribute to the disrupted apoptosis of tumors by caspase-mediated claevage of cytokeratin
-
-
?
additional information
?
-
-
the enzyme catalyzes post-translational modification of protein through the conversion of arginine to citrulline in the presence of calcium ions. Peptidylarginine deiminase-mediated deimination of epithelial cell keratin resulting in cytoskeletal remodeling suggests a possible role for hPADVI in cytoskeletal reorganization in the egg and in early embryo development
-
-
?
additional information
?
-
methylated Arg residues are quite poor substrates for full-length PAD4 in vitro, regardless of whether this residue is in the context of a small molecule or a peptide substrate
-
-
?
additional information
?
-
-
methylated Arg residues are quite poor substrates for full-length PAD4 in vitro, regardless of whether this residue is in the context of a small molecule or a peptide substrate
-
-
?
additional information
?
-
PADI3 expression is driven by Sp1/Sp3 and NF-Y binding to the promoter region
-
-
?
additional information
?
-
-
PADI3 expression is driven by Sp1/Sp3 and NF-Y binding to the promoter region
-
-
?
additional information
?
-
-
the enzyme is involved in autoimmune rheumatoid arthritis
-
-
?
additional information
?
-
the enzyme is involved in autoimmune rheumatoid arthritis
-
-
?
additional information
?
-
the enzyme is involved in autoimmune rheumatoid arthritis
-
-
?
additional information
?
-
the enzyme is involved in autoimmune rheumatoid arthritis
-
-
?
additional information
?
-
the enzyme is involved in autoimmune rheumatoid arthritis
-
-
?
additional information
?
-
-
the enzyme is involved in autoimmune rheumatoid arthritis, isozyme PAD2 is also implicated in neurological diseases with or without ocular manifestations, e.g. multiple sclerosis, autoimmune encephalomyelitis, Alzheimer's disease, and glaucoma
-
-
?
additional information
?
-
the enzyme is involved in autoimmune rheumatoid arthritis, isozyme PAD2 is also implicated in neurological diseases with or without ocular manifestations, e.g. multiple sclerosis, autoimmune encephalomyelitis, Alzheimer's disease, and glaucoma
-
-
?
additional information
?
-
the enzyme is involved in autoimmune rheumatoid arthritis, isozyme PAD2 is also implicated in neurological diseases with or without ocular manifestations, e.g. multiple sclerosis, autoimmune encephalomyelitis, Alzheimer's disease, and glaucoma
-
-
?
additional information
?
-
the enzyme is involved in autoimmune rheumatoid arthritis, isozyme PAD2 is also implicated in neurological diseases with or without ocular manifestations, e.g. multiple sclerosis, autoimmune encephalomyelitis, Alzheimer's disease, and glaucoma
-
-
?
additional information
?
-
the enzyme is involved in autoimmune rheumatoid arthritis, isozyme PAD2 is also implicated in neurological diseases with or without ocular manifestations, e.g. multiple sclerosis, autoimmune encephalomyelitis, Alzheimer's disease, and glaucoma
-
-
?
additional information
?
-
-
no activity with free L-arginine, which is converted by EC 1.14.13.39, nitric oxide synthase, the enzyme is not identical with bacterial arginine deiminase, EC 3.5.3.6, PADs are involved in diverse diseases, e.g. PAD2 in glaucoma of the optic nerve, overview
-
-
?
additional information
?
-
no activity with free L-arginine, which is converted by EC 1.14.13.39, nitric oxide synthase, the enzyme is not identical with bacterial arginine deiminase, EC 3.5.3.6, PADs are involved in diverse diseases, e.g. PAD2 in glaucoma of the optic nerve, overview
-
-
?
additional information
?
-
no activity with free L-arginine, which is converted by EC 1.14.13.39, nitric oxide synthase, the enzyme is not identical with bacterial arginine deiminase, EC 3.5.3.6, PADs are involved in diverse diseases, e.g. PAD2 in glaucoma of the optic nerve, overview
-
-
?
additional information
?
-
no activity with free L-arginine, which is converted by EC 1.14.13.39, nitric oxide synthase, the enzyme is not identical with bacterial arginine deiminase, EC 3.5.3.6, PADs are involved in diverse diseases, e.g. PAD2 in glaucoma of the optic nerve, overview
-
-
?
additional information
?
-
no activity with free L-arginine, which is converted by EC 1.14.13.39, nitric oxide synthase, the enzyme is not identical with bacterial arginine deiminase, EC 3.5.3.6, PADs are involved in diverse diseases, e.g. PAD2 in glaucoma of the optic nerve, overview
-
-
?
additional information
?
-
-
no activity with free L-arginine, which is converted by EC 1.14.13.39, nitric oxide synthase, the enzyme is not identical with bacterial arginine deiminase, EC 3.5.3.6, PADs are involved in diverse diseases, overview
-
-
?
additional information
?
-
no activity with free L-arginine, which is converted by EC 1.14.13.39, nitric oxide synthase, the enzyme is not identical with bacterial arginine deiminase, EC 3.5.3.6, PADs are involved in diverse diseases, overview
-
-
?
additional information
?
-
no activity with free L-arginine, which is converted by EC 1.14.13.39, nitric oxide synthase, the enzyme is not identical with bacterial arginine deiminase, EC 3.5.3.6, PADs are involved in diverse diseases, overview
-
-
?
additional information
?
-
no activity with free L-arginine, which is converted by EC 1.14.13.39, nitric oxide synthase, the enzyme is not identical with bacterial arginine deiminase, EC 3.5.3.6, PADs are involved in diverse diseases, overview
-
-
?
additional information
?
-
no activity with free L-arginine, which is converted by EC 1.14.13.39, nitric oxide synthase, the enzyme is not identical with bacterial arginine deiminase, EC 3.5.3.6, PADs are involved in diverse diseases, overview
-
-
?
additional information
?
-
-
substrate binding structure, sequence specificity, overview
-
-
?
additional information
?
-
substrate binding structure, sequence specificity, overview
-
-
?
additional information
?
-
-
a methylated lysine residue flanking the targeted arginine influences PAD-4-mediated deimination
-
-
?
additional information
?
-
does not citrullinate interleukin-1beta
-
-
?
additional information
?
-
does not citrullinate interleukin-1beta
-
-
?
additional information
?
-
-
no activity toward PVRAK, DQRTA, DVRIA, and EPRLF
-
-
?
additional information
?
-
-
the binding affinity of autodeiminated PAD4 for p53 and H3 are similar to non-deiminated PAD4. In contrast, the affinity of autodeiminated PAD4 for HDAC1, PRMT1, and Cit H3 is reduced by 3.7, 4.5, and 10fold, respectively. Results suggest that the autodeimination of PAD4 in vivo could affect its functional activity by regulating its ability to interact with binding partners
-
-
?
additional information
?
-
-
possible 14-3-3 protein binding sites in human PAD6 are identified by in silico methods. Two of these sites are confirmed as 14-3-3 binding sites by fluorescence polarization competition and X-ray crystallography
-
-
?
additional information
?
-
nearly no activity with free L-arginine, L-agmatine, histone H3, and histone H4
-
-
?
additional information
?
-
isoform PAD2 appears to favor citrullination of glycine-containing motifs. Distinct hydrophobic motifs are identified for PAD2 citrullination sites predicted to reside within alpha-helical and beta-sheet regions. There is potential substrate overlap between coil region citrullination and arginine methylation
-
-
?
additional information
?
-
isoform PAD2 appears to favor citrullination of glycine-containing motifs. Distinct hydrophobic motifs are identified for PAD2 citrullination sites predicted to reside within alpha-helical and beta-sheet regions. There is potential substrate overlap between coil region citrullination and arginine methylation
-
-
?
additional information
?
-
-
isoform PAD2 appears to favor citrullination of glycine-containing motifs. Distinct hydrophobic motifs are identified for PAD2 citrullination sites predicted to reside within alpha-helical and beta-sheet regions. There is potential substrate overlap between coil region citrullination and arginine methylation
-
-
?
additional information
?
-
isoform PAD4 appears to favor citrullination of glycine-containing motifs. There is potential substrate overlap between coil region citrullination and arginine methylation
-
-
?
additional information
?
-
isoform PAD4 appears to favor citrullination of glycine-containing motifs. There is potential substrate overlap between coil region citrullination and arginine methylation
-
-
?
additional information
?
-
-
isoform PAD4 appears to favor citrullination of glycine-containing motifs. There is potential substrate overlap between coil region citrullination and arginine methylation
-
-
?
additional information
?
-
proteomics implicates peptidyl arginine deiminase 2 and optic nerve citrullination in glaucoma pathogenesis. Pressure-induced translational control of PAD2 expression is consistent with a possible role for PAD2 and citrullination in primary open-angle glaucoma
-
-
?
additional information
?
-
-
proteomics implicates peptidyl arginine deiminase 2 and optic nerve citrullination in glaucoma pathogenesis. Pressure-induced translational control of PAD2 expression is consistent with a possible role for PAD2 and citrullination in primary open-angle glaucoma
-
-
?
additional information
?
-
the cortical granule enzyme plays a role in preimplantation embryonic development
-
-
?
additional information
?
-
-
the cortical granule enzyme plays a role in preimplantation embryonic development
-
-
?
additional information
?
-
PAD 6 is essential for oocyte cytoskeletal sheet formation and female fertility, it plays an important role in germ-cell-specific structures in zygote/embryo development, citrullination is a regulator of fertility, overview
-
-
?
additional information
?
-
-
IKKgamma and PAD2 can coimmunoprecipitate in the presence of the Ca2+ ionophore
-
-
?
additional information
?
-
-
does not citrullinate interleukin-1beta
-
-
?
additional information
?
-
-
the enzyme undergoes automodification
-
-
?
additional information
?
-
-
no substrate: peptide GFSPFRSS
-
-
?
additional information
?
-
no substrate: peptide GFSPFRSS
-
-
?
additional information
?
-
no substrates: peptides Arg-Gly-Glu, Met-Arg-Phe, fibrinogen peptide CESSSHHPGIAEFPS-R-GK
-
-
?
additional information
?
-
-
no substrates: peptides Arg-Gly-Glu, Met-Arg-Phe, fibrinogen peptide CESSSHHPGIAEFPS-R-GK
-
-
?
additional information
?
-
-
no substrate: peptide GFSPFRSS
-
-
?
additional information
?
-
no substrate: peptide GFSPFRSS
-
-
?
additional information
?
-
no substrates: peptides Arg-Gly-Glu, Met-Arg-Phe, fibrinogen peptide CESSSHHPGIAEFPS-R-GK
-
-
?
additional information
?
-
-
PAD2 is involved in some processes of neurodegeneration and myelination in the central nervous system
-
-
?
additional information
?
-
-
no activity with free L-arginine, which is converted by EC 1.14.13.39, nitric oxide synthase, the enzyme is not identical with bacterial arginine deiminase, EC 3.5.3.6
-
-
?
additional information
?
-
no activity with free L-arginine, which is converted by EC 1.14.13.39, nitric oxide synthase, the enzyme is not identical with bacterial arginine deiminase, EC 3.5.3.6
-
-
?
additional information
?
-
no activity with free L-arginine, which is converted by EC 1.14.13.39, nitric oxide synthase, the enzyme is not identical with bacterial arginine deiminase, EC 3.5.3.6
-
-
?
additional information
?
-
no activity with free L-arginine, which is converted by EC 1.14.13.39, nitric oxide synthase, the enzyme is not identical with bacterial arginine deiminase, EC 3.5.3.6
-
-
?
additional information
?
-
no activity with free L-arginine, which is converted by EC 1.14.13.39, nitric oxide synthase, the enzyme is not identical with bacterial arginine deiminase, EC 3.5.3.6
-
-
?
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0.11 - 0.18
2-acetyl-Gly-Arg-Gly-Lys-Gly-Gly-Lys-Gly-Leu-Gly-Lys-Gly-Gly-Ala-Lys
0.32 - 0.36
3-acetyl-Arg-Gly-Lys-Gly-Gly-Lys-Gly-Leu-Gly-Lys-Gly-Gly-Ala-Lys
0.3 - 0.95
Ac-Ala-Gly-Arg-Gly-Lys
0.69 - 0.8
Ac-Ser-Ala-Arg-Gly-Lys
0.44 - 1.75
Ac-Ser-Gly-Ala-Gly-Lys-Gly-Gly-Lys-Gly-Leu-Gly-Lys-Gly-Gly-Ala-Ala-Arg-His-Arg-Lys-Val
0.58
Ac-Ser-Gly-Arg-Ala-Lys
-
isozyme PAD1, at 37°C, 10 mM CaCl2, 50 mM NaCl, 100 mM Tris-HCl, pH 7.6, 2 mM dithiothreitol
0.15 - 1.07
Ac-Ser-Gly-Arg-Gly-acetyl-Lys-Gly-Gly-acetyl-Lys-Gly-Leu-Gly-acetyl-Lys-Gly-Gly-Ala-acetyl-Lys
0.37
Ac-Ser-Gly-Arg-Gly-Ala
-
isozyme PAD1, at 37°C, 10 mM CaCl2, 50 mM NaCl, 100 mM Tris-HCl, pH 7.6, 2 mM dithiothreitol
0.86
Ac-Ser-Gly-Arg-Gly-Lys-Gly-Gly-Lys-Gly-Leu-Gly-Lys-Gly
-
isozyme PAD4, at 37°C, 10 mM CaCl2, 50 mM NaCl, 100 mM Tris-HCl, pH 7.6, 2 mM dithiothreitol
0.16 - 0.58
Ac-Ser-Gly-Arg-Gly-Lys-Gly-Gly-Lys-Gly-Leu-Gly-Lys-Gly-Gly-Ala
0.17 - 0.34
Ac-Ser-Gly-Arg-Gly-Lys-Gly-Gly-Lys-Gly-Leu-Gly-Lys-Gly-Gly-Ala-Ala-Arg-His-Arg-Ala-Val
0.43 - 0.66
Ac-Ser-Gly-Arg-Gly-Lys-Gly-Gly-Lys-Gly-Leu-Gly-Lys-Gly-Gly-Ala-Ala-Arg-His-Arg-Lys-Val
0.15 - 0.59
Ac-Ser-Gly-Arg-Gly-Lys-Gly-Gly-Lys-Gly-Leu-Gly-Lys-Gly-Gly-Ala-Lys
1.7
Ac-Ser-Gly-Arg-Gly-Lys-Gly-Gly-Lys-Gly-Leu-Gly-Lys-Gly-Gly-Ala-Lys-Ala-His-Arg-Ala-Val
-
isozyme PAD4, at 37°C, 10 mM CaCl2, 50 mM NaCl, 100 mM Tris-HCl, pH 7.6, 2 mM dithiothreitol
0.22 - 1.95
Ac-Ser-Gly-Arg-Gly-Lys-Gly-Gly-Lys-Gly-Leu-Gly-Lys-Gly-Gly-Ala-Lys-Arg-His
0.48 - 2.16
Ac-Ser-Gly-Arg-Gly-Lys-Gly-Gly-Lys-Gly-Leu-Gly-Lys-Gly-Gly-Ala-Lys-Arg-His-Ala-Ala-Val
0.21 - 0.64
Ac-Ser-Gly-Arg-Gly-Lys-Gly-Gly-Lys-Gly-Leu-Gly-Lys-Gly-Gly-Ala-Lys-Arg-His-Arg-Lys-Val
0.48 - 3.27
Ac-Ser-Gly-Arg-Gly-Lys-Gly-Gly-Lys-Gly-Leu-Gly-Lys-Gly-Gly-Ala-Lys-Lys-His-Lys-Ala-Val
1.19 - 66.3
acetyl-L-Arg methyl ester
0.33 - 0.78
benzoyl-Arg ethyl ester
0.15 - 0.91
benzoyl-Arg-ethyl ester
0.63 - 1.97
benzoyl-Arg-methyl ester
0.16 - 1.23
benzoyl-Arg-NH2
0.028
benzoyl-Gly-L-Arg
-
-
0.0029 - 33.6
benzoyl-L-Arg
0.35 - 7.5
benzoyl-L-Arg ethyl ester
0.53 - 0.54
benzoyl-L-Arg methyl ester
0.152 - 1.39
benzoyl-L-Arg-NH2
0.41
benzoyl-L-arginine
37°C
0.25
benzoyl-L-arginine amide
37°C
1.36
benzoyl-L-arginine ethyl ester
37°C
1.66
benzoyl-L-arginine methyl ester
37°C
11.2
benzoylarginine ethyl ester
-
Km-value of the low activity binding site
0.013 - 0.055
fibrinogen
-
0.001 - 0.064
filaggrin
-
0.14
histone H4-L-arginine
-
isozyme PAD4, at 37°C, 10 mM CaCl2, 50 mM NaCl, 100 mM Tris-HCl, pH 7.6, 2 mM dithiothreitol
0.0074 - 0.038
IHAREIFDSR
0.325
KDRNW
-
in 60 mM MOPS buffer, pH 7.5 and 10 mM CaCl2, at 37°C
23.49 - 26.45
L-Arg methyl ester
0.45
LDRGE
-
in 60 mM MOPS buffer, pH 7.5 and 10 mM CaCl2, at 37°C
0.27
MWRHV
-
in 60 mM MOPS buffer, pH 7.5 and 10 mM CaCl2, at 37°C
0.16 - 17.5
N-alpha-benzoyl-L-arginine amide
0.1 - 30
N-alpha-benzoyl-L-arginine ethyl ester
0.37 - 10.8
N-alpha-benzoyl-L-arginine methyl ester
0.44 - 1.7
Nalpha-benzoyl L-arginine ethyl ester
0.41 - 1.6
Nalpha-benzoyl-L-arginine
0.25 - 0.48
Nalpha-benzoyl-L-arginine amide
0.27 - 2.77
Nalpha-benzoyl-L-arginine ethyl ester
0.24 - 1.66
Nalpha-benzoyl-L-arginine methyl ester
0.372
TKQTARKSTGGK
-
in 60 mM MOPS buffer, pH 7.5 and 10 mM CaCl2, at 37°C
1.63 - 5.61
tosyl-Arg-ethyl ester
0.575
TSTGGRQGSHH
-
in 60 mM MOPS buffer, pH 7.5 and 10 mM CaCl2, at 37°C
0.12
WSRYH
-
in 60 mM MOPS buffer, pH 7.5 and 10 mM CaCl2, at 37°C
0.345
WTRGE
-
in 60 mM MOPS buffer, pH 7.5 and 10 mM CaCl2, at 37°C
0.187
YWRDH
-
in 60 mM MOPS buffer, pH 7.5 and 10 mM CaCl2, at 37°C
0.088 - 0.35
[histone H4]-L-Arg
0.11
2-acetyl-Gly-Arg-Gly-Lys-Gly-Gly-Lys-Gly-Leu-Gly-Lys-Gly-Gly-Ala-Lys
-
isozyme PAD1, at 37°C, 10 mM CaCl2, 50 mM NaCl, 100 mM Tris-HCl, pH 7.6, 2 mM dithiothreitol
0.18
2-acetyl-Gly-Arg-Gly-Lys-Gly-Gly-Lys-Gly-Leu-Gly-Lys-Gly-Gly-Ala-Lys
-
isozyme PAD4, at 37°C, 10 mM CaCl2, 50 mM NaCl, 100 mM Tris-HCl, pH 7.6, 2 mM dithiothreitol
0.32
3-acetyl-Arg-Gly-Lys-Gly-Gly-Lys-Gly-Leu-Gly-Lys-Gly-Gly-Ala-Lys
-
isozyme PAD4, at 37°C, 10 mM CaCl2, 50 mM NaCl, 100 mM Tris-HCl, pH 7.6, 2 mM dithiothreitol
0.36
3-acetyl-Arg-Gly-Lys-Gly-Gly-Lys-Gly-Leu-Gly-Lys-Gly-Gly-Ala-Lys
-
isozyme PAD1, at 37°C, 10 mM CaCl2, 50 mM NaCl, 100 mM Tris-HCl, pH 7.6, 2 mM dithiothreitol
0.3
Ac-Ala-Gly-Arg-Gly-Lys
-
isozyme PAD1, at 37°C, 10 mM CaCl2, 50 mM NaCl, 100 mM Tris-HCl, pH 7.6, 2 mM dithiothreitol
0.95
Ac-Ala-Gly-Arg-Gly-Lys
-
isozyme PAD3, at 37°C, 10 mM CaCl2, 50 mM NaCl, 100 mM Tris-HCl, pH 7.6, 2 mM dithiothreitol
0.69
Ac-Ser-Ala-Arg-Gly-Lys
-
isozyme PAD3, at 37°C, 10 mM CaCl2, 50 mM NaCl, 100 mM Tris-HCl, pH 7.6, 2 mM dithiothreitol
0.8
Ac-Ser-Ala-Arg-Gly-Lys
-
isozyme PAD1, at 37°C, 10 mM CaCl2, 50 mM NaCl, 100 mM Tris-HCl, pH 7.6, 2 mM dithiothreitol
0.44
Ac-Ser-Gly-Ala-Gly-Lys-Gly-Gly-Lys-Gly-Leu-Gly-Lys-Gly-Gly-Ala-Ala-Arg-His-Arg-Lys-Val
-
isozyme PAD1, at 37°C, 10 mM CaCl2, 50 mM NaCl, 100 mM Tris-HCl, pH 7.6, 2 mM dithiothreitol
1.75
Ac-Ser-Gly-Ala-Gly-Lys-Gly-Gly-Lys-Gly-Leu-Gly-Lys-Gly-Gly-Ala-Ala-Arg-His-Arg-Lys-Val
-
isozyme PAD4, at 37°C, 10 mM CaCl2, 50 mM NaCl, 100 mM Tris-HCl, pH 7.6, 2 mM dithiothreitol
0.15
Ac-Ser-Gly-Arg-Gly-acetyl-Lys-Gly-Gly-acetyl-Lys-Gly-Leu-Gly-acetyl-Lys-Gly-Gly-Ala-acetyl-Lys
-
isozyme PAD1, at 37°C, 10 mM CaCl2, 50 mM NaCl, 100 mM Tris-HCl, pH 7.6, 2 mM dithiothreitol
0.59
Ac-Ser-Gly-Arg-Gly-acetyl-Lys-Gly-Gly-acetyl-Lys-Gly-Leu-Gly-acetyl-Lys-Gly-Gly-Ala-acetyl-Lys
-
isozyme PAD3, at 37°C, 10 mM CaCl2, 50 mM NaCl, 100 mM Tris-HCl, pH 7.6, 2 mM dithiothreitol
1.07
Ac-Ser-Gly-Arg-Gly-acetyl-Lys-Gly-Gly-acetyl-Lys-Gly-Leu-Gly-acetyl-Lys-Gly-Gly-Ala-acetyl-Lys
-
isozyme PAD4, at 37°C, 10 mM CaCl2, 50 mM NaCl, 100 mM Tris-HCl, pH 7.6, 2 mM dithiothreitol
0.16
Ac-Ser-Gly-Arg-Gly-Lys-Gly-Gly-Lys-Gly-Leu-Gly-Lys-Gly-Gly-Ala
-
isozyme PAD1, at 37°C, 10 mM CaCl2, 50 mM NaCl, 100 mM Tris-HCl, pH 7.6, 2 mM dithiothreitol
0.58
Ac-Ser-Gly-Arg-Gly-Lys-Gly-Gly-Lys-Gly-Leu-Gly-Lys-Gly-Gly-Ala
-
isozyme PAD4, at 37°C, 10 mM CaCl2, 50 mM NaCl, 100 mM Tris-HCl, pH 7.6, 2 mM dithiothreitol
0.17
Ac-Ser-Gly-Arg-Gly-Lys-Gly-Gly-Lys-Gly-Leu-Gly-Lys-Gly-Gly-Ala-Ala-Arg-His-Arg-Ala-Val
-
isozyme PAD4, at 37°C, 10 mM CaCl2, 50 mM NaCl, 100 mM Tris-HCl, pH 7.6, 2 mM dithiothreitol
0.34
Ac-Ser-Gly-Arg-Gly-Lys-Gly-Gly-Lys-Gly-Leu-Gly-Lys-Gly-Gly-Ala-Ala-Arg-His-Arg-Ala-Val
-
isozyme PAD1, at 37°C, 10 mM CaCl2, 50 mM NaCl, 100 mM Tris-HCl, pH 7.6, 2 mM dithiothreitol
0.43
Ac-Ser-Gly-Arg-Gly-Lys-Gly-Gly-Lys-Gly-Leu-Gly-Lys-Gly-Gly-Ala-Ala-Arg-His-Arg-Lys-Val
-
isozyme PAD4, at 37°C, 10 mM CaCl2, 50 mM NaCl, 100 mM Tris-HCl, pH 7.6, 2 mM dithiothreitol
0.66
Ac-Ser-Gly-Arg-Gly-Lys-Gly-Gly-Lys-Gly-Leu-Gly-Lys-Gly-Gly-Ala-Ala-Arg-His-Arg-Lys-Val
-
isozyme PAD1, at 37°C, 10 mM CaCl2, 50 mM NaCl, 100 mM Tris-HCl, pH 7.6, 2 mM dithiothreitol
0.15
Ac-Ser-Gly-Arg-Gly-Lys-Gly-Gly-Lys-Gly-Leu-Gly-Lys-Gly-Gly-Ala-Lys
-
isozyme PAD3, at 37°C, 10 mM CaCl2, 50 mM NaCl, 100 mM Tris-HCl, pH 7.6, 2 mM dithiothreitol
0.2
Ac-Ser-Gly-Arg-Gly-Lys-Gly-Gly-Lys-Gly-Leu-Gly-Lys-Gly-Gly-Ala-Lys
-
isozyme PAD1, at 37°C, 10 mM CaCl2, 50 mM NaCl, 100 mM Tris-HCl, pH 7.6, 2 mM dithiothreitol
0.59
Ac-Ser-Gly-Arg-Gly-Lys-Gly-Gly-Lys-Gly-Leu-Gly-Lys-Gly-Gly-Ala-Lys
-
isozyme PAD4, at 37°C, 10 mM CaCl2, 50 mM NaCl, 100 mM Tris-HCl, pH 7.6, 2 mM dithiothreitol
0.22
Ac-Ser-Gly-Arg-Gly-Lys-Gly-Gly-Lys-Gly-Leu-Gly-Lys-Gly-Gly-Ala-Lys-Arg-His
-
isozyme PAD1, at 37°C, 10 mM CaCl2, 50 mM NaCl, 100 mM Tris-HCl, pH 7.6, 2 mM dithiothreitol
0.52
Ac-Ser-Gly-Arg-Gly-Lys-Gly-Gly-Lys-Gly-Leu-Gly-Lys-Gly-Gly-Ala-Lys-Arg-His
-
isozyme PAD4, at 37°C, 10 mM CaCl2, 50 mM NaCl, 100 mM Tris-HCl, pH 7.6, 2 mM dithiothreitol
1.95
Ac-Ser-Gly-Arg-Gly-Lys-Gly-Gly-Lys-Gly-Leu-Gly-Lys-Gly-Gly-Ala-Lys-Arg-His
-
isozyme PAD3, at 37°C, 10 mM CaCl2, 50 mM NaCl, 100 mM Tris-HCl, pH 7.6, 2 mM dithiothreitol
0.48
Ac-Ser-Gly-Arg-Gly-Lys-Gly-Gly-Lys-Gly-Leu-Gly-Lys-Gly-Gly-Ala-Lys-Arg-His-Ala-Ala-Val
-
isozyme PAD1, at 37°C, 10 mM CaCl2, 50 mM NaCl, 100 mM Tris-HCl, pH 7.6, 2 mM dithiothreitol
2.16
Ac-Ser-Gly-Arg-Gly-Lys-Gly-Gly-Lys-Gly-Leu-Gly-Lys-Gly-Gly-Ala-Lys-Arg-His-Ala-Ala-Val
-
isozyme PAD4, at 37°C, 10 mM CaCl2, 50 mM NaCl, 100 mM Tris-HCl, pH 7.6, 2 mM dithiothreitol
0.21
Ac-Ser-Gly-Arg-Gly-Lys-Gly-Gly-Lys-Gly-Leu-Gly-Lys-Gly-Gly-Ala-Lys-Arg-His-Arg-Lys-Val
-
isozyme PAD1, at 37°C, 10 mM CaCl2, 50 mM NaCl, 100 mM Tris-HCl, pH 7.6, 2 mM dithiothreitol
0.42
Ac-Ser-Gly-Arg-Gly-Lys-Gly-Gly-Lys-Gly-Leu-Gly-Lys-Gly-Gly-Ala-Lys-Arg-His-Arg-Lys-Val
-
isozyme PAD3, at 37°C, 10 mM CaCl2, 50 mM NaCl, 100 mM Tris-HCl, pH 7.6, 2 mM dithiothreitol
0.64
Ac-Ser-Gly-Arg-Gly-Lys-Gly-Gly-Lys-Gly-Leu-Gly-Lys-Gly-Gly-Ala-Lys-Arg-His-Arg-Lys-Val
-
isozyme PAD4, at 37°C, 10 mM CaCl2, 50 mM NaCl, 100 mM Tris-HCl, pH 7.6, 2 mM dithiothreitol
0.48
Ac-Ser-Gly-Arg-Gly-Lys-Gly-Gly-Lys-Gly-Leu-Gly-Lys-Gly-Gly-Ala-Lys-Lys-His-Lys-Ala-Val
-
isozyme PAD1, at 37°C, 10 mM CaCl2, 50 mM NaCl, 100 mM Tris-HCl, pH 7.6, 2 mM dithiothreitol
0.86
Ac-Ser-Gly-Arg-Gly-Lys-Gly-Gly-Lys-Gly-Leu-Gly-Lys-Gly-Gly-Ala-Lys-Lys-His-Lys-Ala-Val
-
isozyme PAD3, at 37°C, 10 mM CaCl2, 50 mM NaCl, 100 mM Tris-HCl, pH 7.6, 2 mM dithiothreitol
3.27
Ac-Ser-Gly-Arg-Gly-Lys-Gly-Gly-Lys-Gly-Leu-Gly-Lys-Gly-Gly-Ala-Lys-Lys-His-Lys-Ala-Val
-
isozyme PAD4, at 37°C, 10 mM CaCl2, 50 mM NaCl, 100 mM Tris-HCl, pH 7.6, 2 mM dithiothreitol
0.91
acetyl-L-Arg
pH 7.6, 55°C, peptidylarginine deiminase 1
10.67
acetyl-L-Arg
-
recombinant enzyme
11.3
acetyl-L-Arg
-
native enzyme
1.19
acetyl-L-Arg methyl ester
pH 7.6, 55°C, peptidylarginine deiminase 1
2.96
acetyl-L-Arg methyl ester
-
native enzyme
3.24
acetyl-L-Arg methyl ester
-
recombinant enzyme
3.73
acetyl-L-Arg methyl ester
pH 7.6, 55°C, peptidylarginine deiminase 2
66.3
acetyl-L-Arg methyl ester
pH 7.6, 55°C, peptidylarginine deiminase 3
0.33
benzoyl-Arg ethyl ester
-
-
0.5
benzoyl-Arg ethyl ester
-
native enzyme
0.54
benzoyl-Arg ethyl ester
-
recombinant enzyme
0.78
benzoyl-Arg ethyl ester
-
Km-value of the high activity binding site
0.15
benzoyl-Arg-ethyl ester
-
pH 7.6, 37°C, hPADI2 expressed by baculovirus
0.73
benzoyl-Arg-ethyl ester
-
pH 7.6, 37°C, hPADI2 expressed in Escherichia coli
0.91
benzoyl-Arg-ethyl ester
-
pH 7.6, 37°C, hPADI4 expressed in Escherichia coli
0.63
benzoyl-Arg-methyl ester
-
pH 7.6, 37°C, hPADI4 expressed in Escherichia coli
0.77
benzoyl-Arg-methyl ester
-
pH 7.6, 37°C, hPADI2 expressed by baculovirus
1.97
benzoyl-Arg-methyl ester
-
pH 7.6, 37°C, hPADI2 expressed in Escherichia coli
0.16
benzoyl-Arg-NH2
-
pH 7.6, 37°C, hPADI4 expressed in Escherichia coli
1.01
benzoyl-Arg-NH2
-
pH 7.6, 37°C, hPADI2 expressed in Escherichia coli
1.23
benzoyl-Arg-NH2
-
pH 7.6, 37°C, hPADI2 expressed by baculovirus
0.0029
benzoyl-L-Arg
-
-
0.38
benzoyl-L-Arg
pH 7.6, 55°C, peptidylarginine deiminase 1
1.49
benzoyl-L-Arg
pH 7.6, 55°C, peptidylarginine deiminase 2
2.28
benzoyl-L-Arg
-
native enzyme
2.58
benzoyl-L-Arg
-
recombinant enzyme
33.6
benzoyl-L-Arg
pH 7.6, 55°C, peptidylarginine deiminase 3
0.35
benzoyl-L-Arg ethyl ester
pH 7.6, 55°C, peptidylarginine deiminase 1
0.5
benzoyl-L-Arg ethyl ester
pH 7.6, 55°C, peptidylarginine deiminase 2
0.874
benzoyl-L-Arg ethyl ester
-
-
7.5
benzoyl-L-Arg ethyl ester
pH 7.6, 55°C, peptidylarginine deiminase 3
0.53
benzoyl-L-Arg methyl ester
-
recombinant enzyme
0.54
benzoyl-L-Arg methyl ester
-
native enzyme
0.152
benzoyl-L-Arg-NH2
-
-
1.37
benzoyl-L-Arg-NH2
-
native enzyme
1.39
benzoyl-L-Arg-NH2
-
recombinant enzyme
0.013
fibrinogen
-
hPADI2
-
0.055
fibrinogen
-
hPADI4
-
0.001
filaggrin
-
hPADI2
-
0.064
filaggrin
-
hPADI4
-
0.0074
IHAREIFDSR
pH 7.5, 37°C
0.038
IHAREIFDSR
pH 7.5, 37°C
0.141
L-Arg
-
-
33.4
L-Arg
-
native enzyme
36.3
L-Arg
-
recombinant enzyme
23.49
L-Arg methyl ester
-
recombinant enzyme
26.45
L-Arg methyl ester
-
native enzyme
0.16
N-alpha-benzoyl-L-arginine amide
-
isozyme PAD1, at 37°C, 10 mM CaCl2, 50 mM NaCl, 100 mM Tris-HCl, pH 7.6, 2 mM dithiothreitol
0.25
N-alpha-benzoyl-L-arginine amide
-
isozyme PAD4, at 37°C, 10 mM CaCl2, 50 mM NaCl, 100 mM Tris-HCl, pH 7.6, 2 mM dithiothreitol
13.9
N-alpha-benzoyl-L-arginine amide
-
isozyme PAD3, at 37°C, 10 mM CaCl2, 50 mM NaCl, 100 mM Tris-HCl, pH 7.6, 2 mM dithiothreitol
17.5
N-alpha-benzoyl-L-arginine amide
-
mutant PAD3 enzyme G374R, at 37°C, 10 mM CaCl2, 50 mM NaCl, 100 mM Tris-HCl, pH 7.6, 2 mM dithiothreitol
0.1
N-alpha-benzoyl-L-arginine ethyl ester
mutant F221A/F222A, pH 7.6, 37°C
0.1
N-alpha-benzoyl-L-arginine ethyl ester
mutant V469T, pH 7.5, 25°C
0.1
N-alpha-benzoyl-L-arginine ethyl ester
pH 7.6, 37°C, mutant enzyme F221A/F222A
0.12
N-alpha-benzoyl-L-arginine ethyl ester
mutant D123N, pH 7.6, 37°C
0.12
N-alpha-benzoyl-L-arginine ethyl ester
pH 7.6, 37°C, mutant enzyme D123N
0.14
N-alpha-benzoyl-L-arginine ethyl ester
mutant D125A, pH 7.6, 37°C
0.14
N-alpha-benzoyl-L-arginine ethyl ester
pH 7.6, 37°C, mutant enzyme D125A
0.16
N-alpha-benzoyl-L-arginine ethyl ester
wild-type, pH 7.6, 37°C
0.16
N-alpha-benzoyl-L-arginine ethyl ester
pH 7.6, 37°C, wild-type enzyme
0.19
N-alpha-benzoyl-L-arginine ethyl ester
-
isozyme PAD1, at 37°C, 10 mM CaCl2, 50 mM NaCl, 100 mM Tris-HCl, pH 7.6, 2 mM dithiothreitol
0.25
N-alpha-benzoyl-L-arginine ethyl ester
mutant D169A, pH 7.6, 37°C
0.25
N-alpha-benzoyl-L-arginine ethyl ester
pH 7.6, 37°C, mutant enzyme D169A
0.3
N-alpha-benzoyl-L-arginine ethyl ester
mutant W548K, pH 7.5, 25°C
0.33
N-alpha-benzoyl-L-arginine ethyl ester
mutant D166A, pH 7.6, 37°C
0.33
N-alpha-benzoyl-L-arginine ethyl ester
pH 7.6, 37°C, mutant enzyme D166A
0.4
N-alpha-benzoyl-L-arginine ethyl ester
mutant C434A, pH 7.5, 25°C
0.4
N-alpha-benzoyl-L-arginine ethyl ester
mutant D465A, pH 7.5, 25°C
0.4
N-alpha-benzoyl-L-arginine ethyl ester
mutant F576A, pH 7.5, 25°C
0.4
N-alpha-benzoyl-L-arginine ethyl ester
mutant L279A, pH 7.5, 25°C
0.4
N-alpha-benzoyl-L-arginine ethyl ester
mutant L279I, pH 7.5, 25°C
0.4
N-alpha-benzoyl-L-arginine ethyl ester
mutant R374Q, pH 7.5, 25°C
0.5
N-alpha-benzoyl-L-arginine ethyl ester
wild-type, pH 7.5, 25°C
0.5
N-alpha-benzoyl-L-arginine ethyl ester
mutant L6D, pH 7.5, 25°C
0.5
N-alpha-benzoyl-L-arginine ethyl ester
mutant L6I, pH 7.5, 25°C
0.5
N-alpha-benzoyl-L-arginine ethyl ester
mutant V283I, pH 7.5, 25°C
0.5
N-alpha-benzoyl-L-arginine ethyl ester
mutant V284I, pH 7.5, 25°C
0.6
N-alpha-benzoyl-L-arginine ethyl ester
mutant D389A, pH 7.6, 37°C
0.6
N-alpha-benzoyl-L-arginine ethyl ester
mutant L6A, pH 7.5, 25°C
0.6
N-alpha-benzoyl-L-arginine ethyl ester
pH 7.6, 37°C, mutant enzyme D389A
0.7
N-alpha-benzoyl-L-arginine ethyl ester
mutant F541A, pH 7.5, 25°C
0.7
N-alpha-benzoyl-L-arginine ethyl ester
mutant R374A, pH 7.5, 25°C
0.7
N-alpha-benzoyl-L-arginine ethyl ester
mutant R639A, pH 7.5, 25°C
0.7
N-alpha-benzoyl-L-arginine ethyl ester
mutant V283A, pH 7.5, 25°C
0.8
N-alpha-benzoyl-L-arginine ethyl ester
mutant F285A, pH 7.5, 25°C
0.9
N-alpha-benzoyl-L-arginine ethyl ester
mutant V283T, pH 7.5, 25°C
0.9
N-alpha-benzoyl-L-arginine ethyl ester
mutant V284D, pH 7.5, 25°C
1
N-alpha-benzoyl-L-arginine ethyl ester
mutant V469L, pH 7.5, 25°C
1.36
N-alpha-benzoyl-L-arginine ethyl ester
-
isozyme PAD4, at 37°C, 10 mM CaCl2, 50 mM NaCl, 100 mM Tris-HCl, pH 7.6, 2 mM dithiothreitol
1.4
N-alpha-benzoyl-L-arginine ethyl ester
mutant W548F, pH 7.5, 25°C
1.5
N-alpha-benzoyl-L-arginine ethyl ester
mutant V284A, pH 7.5, 25°C
1.7
N-alpha-benzoyl-L-arginine ethyl ester
mutant R441A, pH 7.5, 25°C
1.9
N-alpha-benzoyl-L-arginine ethyl ester
mutant L279D, pH 7.5, 25°C
2.3
N-alpha-benzoyl-L-arginine ethyl ester
mutant R372Q, pH 7.5, 25°C
2.4
N-alpha-benzoyl-L-arginine ethyl ester
mutant D177A, pH 7.6, 37°C
2.4
N-alpha-benzoyl-L-arginine ethyl ester
pH 7.6, 37°C, mutant enzyme D177A
3.2
N-alpha-benzoyl-L-arginine ethyl ester
mutant Y435A, pH 7.5, 25°C
3.4
N-alpha-benzoyl-L-arginine ethyl ester
mutant E352A, pH 7.6, 37°C
3.4
N-alpha-benzoyl-L-arginine ethyl ester
pH 7.6, 37°C, mutant enzyme E352A
3.7
N-alpha-benzoyl-L-arginine ethyl ester
mutant R372A, pH 7.5, 25°C
4.4
N-alpha-benzoyl-L-arginine ethyl ester
mutant R347A, pH 7.6, 37°C
4.4
N-alpha-benzoyl-L-arginine ethyl ester
pH 7.6, 37°C, mutant enzyme R347
5
N-alpha-benzoyl-L-arginine ethyl ester
mutant D370A, pH 7.6, 37°C
5
N-alpha-benzoyl-L-arginine ethyl ester
pH 7.6, 37°C, mutant enzyme D370A
5.3
N-alpha-benzoyl-L-arginine ethyl ester
mutant V469A, pH 7.5, 25°C
6.3
N-alpha-benzoyl-L-arginine ethyl ester
mutant Q350A, pH 7.6, 37°C
6.3
N-alpha-benzoyl-L-arginine ethyl ester
pH 7.6, 37°C, mutant enzyme Q350A
8
N-alpha-benzoyl-L-arginine ethyl ester
mutant D374A, pH 7.6, 37°C
8
N-alpha-benzoyl-L-arginine ethyl ester
pH 7.6, 37°C, mutant enzyme D374A
13
N-alpha-benzoyl-L-arginine ethyl ester
mutant E412A, pH 7.6, 37°C
13
N-alpha-benzoyl-L-arginine ethyl ester
pH 7.6, 37°C, mutant enzyme E412A
30
N-alpha-benzoyl-L-arginine ethyl ester
mutant R373A, pH 7.6, 37°C
30
N-alpha-benzoyl-L-arginine ethyl ester
pH 7.6, 37°C, mutant enzyme W373A
0.37
N-alpha-benzoyl-L-arginine methyl ester
-
isozyme PAD1, at 37°C, 10 mM CaCl2, 50 mM NaCl, 100 mM Tris-HCl, pH 7.6, 2 mM dithiothreitol
1.66
N-alpha-benzoyl-L-arginine methyl ester
-
isozyme PAD4, at 37°C, 10 mM CaCl2, 50 mM NaCl, 100 mM Tris-HCl, pH 7.6, 2 mM dithiothreitol
10.8
N-alpha-benzoyl-L-arginine methyl ester
-
isozyme PAD3, at 37°C, 10 mM CaCl2, 50 mM NaCl, 100 mM Tris-HCl, pH 7.6, 2 mM dithiothreitol
0.44
Nalpha-benzoyl L-arginine ethyl ester
-
37°C, pH 7.6, mutant S55G
0.45
Nalpha-benzoyl L-arginine ethyl ester
-
37°C, pH 7.6, mutant R484K
0.45
Nalpha-benzoyl L-arginine ethyl ester
-
37°C, pH 7.6, mutant R484Q
0.48
Nalpha-benzoyl L-arginine ethyl ester
-
37°C, pH 7.6, mutant R205Q
0.5
Nalpha-benzoyl L-arginine ethyl ester
-
37°C, pH 7.6, mutant R156K
0.58
Nalpha-benzoyl L-arginine ethyl ester
-
37°C, pH 7.6, wild-type
0.69
Nalpha-benzoyl L-arginine ethyl ester
-
37°C, pH 7.6, mutant R639K
0.72
Nalpha-benzoyl L-arginine ethyl ester
-
37°C, pH 7.6, mutant R419K
0.8
Nalpha-benzoyl L-arginine ethyl ester
-
37°C, pH 7.6, mutant A112G
0.86
Nalpha-benzoyl L-arginine ethyl ester
-
37°C, pH 7.6, mutant R123K
0.87
Nalpha-benzoyl L-arginine ethyl ester
-
37°C, pH 7.6, mutant R419Q
0.89
Nalpha-benzoyl L-arginine ethyl ester
-
37°C, pH 7.6, mutant R609Q
1.2
Nalpha-benzoyl L-arginine ethyl ester
-
37°C, pH 7.6, mutant R374Q
1.2
Nalpha-benzoyl L-arginine ethyl ester
-
37°C, pH 7.6, mutant R639Q
1.35
Nalpha-benzoyl L-arginine ethyl ester
-
37°C, pH 7.6, mutant A82V
1.4
Nalpha-benzoyl L-arginine ethyl ester
-
37°C, pH 7.6, mutant R123Q
1.5
Nalpha-benzoyl L-arginine ethyl ester
-
37°C, pH 7.6, mutant R205K
1.5
Nalpha-benzoyl L-arginine ethyl ester
-
37°C, pH 7.6, mutant R609K
1.6
Nalpha-benzoyl L-arginine ethyl ester
-
37°C, pH 7.6, mutant R156Q
1.7
Nalpha-benzoyl L-arginine ethyl ester
-
37°C, pH 7.6, mutant R374K
0.41
Nalpha-benzoyl-L-arginine
at pH 7.5 and 37°C
1.6
Nalpha-benzoyl-L-arginine
at pH 7.6 and 37°C
0.25
Nalpha-benzoyl-L-arginine amide
at pH 7.5 and 37°C
0.48
Nalpha-benzoyl-L-arginine amide
at pH 7.6 and 37°C
0.27
Nalpha-benzoyl-L-arginine ethyl ester
at pH 7.6 and 37°C
0.31
Nalpha-benzoyl-L-arginine ethyl ester
-
wild-type, pH 7.4, 25°C
0.35
Nalpha-benzoyl-L-arginine ethyl ester
-
mutant D273A/R544A, pH 7.4, 25°C
0.36
Nalpha-benzoyl-L-arginine ethyl ester
-
mutant D547A, pH 7.4, 25°C
0.36
Nalpha-benzoyl-L-arginine ethyl ester
-
mutant Y237A, pH 7.4, 25°C
0.38
Nalpha-benzoyl-L-arginine ethyl ester
-
mutant Y237A/E281A, pH 7.4, 25°C
0.4
Nalpha-benzoyl-L-arginine ethyl ester
-
mutant R8A/D547A, pH 7.4, 25°C
0.45
Nalpha-benzoyl-L-arginine ethyl ester
-
mutant E281A, pH 7.4, 25°C
0.47
Nalpha-benzoyl-L-arginine ethyl ester
-
mutant R8A, pH 7.4, 25°C
0.47
Nalpha-benzoyl-L-arginine ethyl ester
-
mutant R8H, pH 7.4, 25°C
0.5
Nalpha-benzoyl-L-arginine ethyl ester
-
mutant R8K, pH 7.4, 25°C
0.6
Nalpha-benzoyl-L-arginine ethyl ester
-
mutant R8Q , pH 7.4, 25°C
0.72
Nalpha-benzoyl-L-arginine ethyl ester
-
mutant D547E, pH 7.4, 25°C
0.86
Nalpha-benzoyl-L-arginine ethyl ester
-
mutant D547N, pH 7.4, 25°C
1.06
Nalpha-benzoyl-L-arginine ethyl ester
-
mutant R8E, pH 7.4, 25°C
1.35
Nalpha-benzoyl-L-arginine ethyl ester
-
pH 7.5, 68°C
1.36
Nalpha-benzoyl-L-arginine ethyl ester
at pH 7.5 and 37°C
1.36
Nalpha-benzoyl-L-arginine ethyl ester
-
mutant R8L, pH 7.4, 25°C
2.33
Nalpha-benzoyl-L-arginine ethyl ester
-
mutant Y435A, pH 7.4, 25°C
2.73
Nalpha-benzoyl-L-arginine ethyl ester
-
mutant Y435N, pH 7.4, 25°C
2.77
Nalpha-benzoyl-L-arginine ethyl ester
-
mutant R8E/D547E, pH 7.4, 25°C
0.24
Nalpha-benzoyl-L-arginine methyl ester
at pH 7.6 and 37°C
1.66
Nalpha-benzoyl-L-arginine methyl ester
at pH 7.5 and 37°C
0.0054
PPGFSPFR
pH 7.5, 37°C
0.045
PPGFSPFR
pH 7.5, 37°C
1.63
tosyl-Arg-ethyl ester
-
pH 7.6, 37°C, hPADI2 expressed by baculovirus
5.61
tosyl-Arg-ethyl ester
-
pH 7.6, 37°C, hPADI2 expressed in Escherichia coli
0.088
[histone H4]-L-Arg
-
37°C, pH 7.6, mutant R374K
0.094
[histone H4]-L-Arg
-
37°C, pH 7.6, mutant R639Q
0.1
[histone H4]-L-Arg
-
37°C, pH 7.6, mutant R123Q
0.11
[histone H4]-L-Arg
-
37°C, pH 7.6, mutant R156Q
0.14
[histone H4]-L-Arg
-
37°C, pH 7.6, mutant R419K
0.15
[histone H4]-L-Arg
-
37°C, pH 7.6, mutant R374Q
0.15
[histone H4]-L-Arg
-
37°C, pH 7.6, mutant R639K
0.17
[histone H4]-L-Arg
-
37°C, pH 7.6, mutant A112G
0.18
[histone H4]-L-Arg
-
37°C, pH 7.6, mutant R156K
0.18
[histone H4]-L-Arg
-
37°C, pH 7.6, mutant R609Q
0.2
[histone H4]-L-Arg
-
37°C, pH 7.6, mutant S55G
0.21
[histone H4]-L-Arg
-
37°C, pH 7.6, wild-type
0.22
[histone H4]-L-Arg
-
37°C, pH 7.6, mutant R419Q
0.24
[histone H4]-L-Arg
-
37°C, pH 7.6, mutant R123K
0.26
[histone H4]-L-Arg
-
37°C, pH 7.6, mutant R484K
0.3
[histone H4]-L-Arg
-
37°C, pH 7.6, mutant R484Q
0.35
[histone H4]-L-Arg
-
37°C, pH 7.6, mutant A82V
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
0.45
2-acetyl-Gly-Arg-Gly-Lys-Gly-Gly-Lys-Gly-Leu-Gly-Lys-Gly-Gly-Ala-Lys
-
isozyme PAD1, at 37°C, 10 mM CaCl2, 50 mM NaCl, 100 mM Tris-HCl, pH 7.6, 2 mM dithiothreitol
0.48
3-acetyl-Arg-Gly-Lys-Gly-Gly-Lys-Gly-Leu-Gly-Lys-Gly-Gly-Ala-Lys
-
isozyme PAD1, at 37°C, 10 mM CaCl2, 50 mM NaCl, 100 mM Tris-HCl, pH 7.6, 2 mM dithiothreitol
0.21 - 0.59
Ac-Ala-Gly-Arg-Gly-Lys
0.62 - 1.13
Ac-Ser-Ala-Arg-Gly-Lys
1.46
Ac-Ser-Gly-Ala-Gly-Lys-Gly-Gly-Lys-Gly-Leu-Gly-Lys-Gly-Gly-Ala-Ala-Arg-His-Arg-Lys-Val
-
isozyme PAD1, at 37°C, 10 mM CaCl2, 50 mM NaCl, 100 mM Tris-HCl, pH 7.6, 2 mM dithiothreitol
0.98
Ac-Ser-Gly-Arg-Ala-Lys
-
isozyme PAD1, at 37°C, 10 mM CaCl2, 50 mM NaCl, 100 mM Tris-HCl, pH 7.6, 2 mM dithiothreitol
0.57 - 1.08
Ac-Ser-Gly-Arg-Gly-acetyl-Lys-Gly-Gly-acetyl-Lys-Gly-Leu-Gly-acetyl-Lys-Gly-Gly-Ala-acetyl-Lys
0.93
Ac-Ser-Gly-Arg-Gly-Ala
-
isozyme PAD1, at 37°C, 10 mM CaCl2, 50 mM NaCl, 100 mM Tris-HCl, pH 7.6, 2 mM dithiothreitol
2.83
Ac-Ser-Gly-Arg-Gly-Lys-Gly-Gly-Lys-Gly-Leu-Gly-Lys-Gly
-
isozyme PAD4, at 37°C, 10 mM CaCl2, 50 mM NaCl, 100 mM Tris-HCl, pH 7.6, 2 mM dithiothreitol
0.75 - 2.53
Ac-Ser-Gly-Arg-Gly-Lys-Gly-Gly-Lys-Gly-Leu-Gly-Lys-Gly-Gly-Ala
0.77
Ac-Ser-Gly-Arg-Gly-Lys-Gly-Gly-Lys-Gly-Leu-Gly-Lys-Gly-Gly-Ala-Ala-Arg-His-Arg-Ala-Val
-
isozyme PAD1, at 37°C, 10 mM CaCl2, 50 mM NaCl, 100 mM Tris-HCl, pH 7.6, 2 mM dithiothreitol
0.87
Ac-Ser-Gly-Arg-Gly-Lys-Gly-Gly-Lys-Gly-Leu-Gly-Lys-Gly-Gly-Ala-Ala-Arg-His-Arg-Lys-Val
-
isozyme PAD1, at 37°C, 10 mM CaCl2, 50 mM NaCl, 100 mM Tris-HCl, pH 7.6, 2 mM dithiothreitol
0.08 - 2.3
Ac-Ser-Gly-Arg-Gly-Lys-Gly-Gly-Lys-Gly-Leu-Gly-Lys-Gly-Gly-Ala-Lys
0.64 - 4.92
Ac-Ser-Gly-Arg-Gly-Lys-Gly-Gly-Lys-Gly-Leu-Gly-Lys-Gly-Gly-Ala-Lys-Arg-His
1.83
Ac-Ser-Gly-Arg-Gly-Lys-Gly-Gly-Lys-Gly-Leu-Gly-Lys-Gly-Gly-Ala-Lys-Arg-His-Ala-Ala-Val
-
isozyme PAD1, at 37°C, 10 mM CaCl2, 50 mM NaCl, 100 mM Tris-HCl, pH 7.6, 2 mM dithiothreitol
0.31 - 3.39
Ac-Ser-Gly-Arg-Gly-Lys-Gly-Gly-Lys-Gly-Leu-Gly-Lys-Gly-Gly-Ala-Lys-Arg-His-Arg-Lys-Val
0.34 - 0.56
Ac-Ser-Gly-Arg-Gly-Lys-Gly-Gly-Lys-Gly-Leu-Gly-Lys-Gly-Gly-Ala-Lys-Lys-His-Lys-Ala-Val
0.32 - 13.68
acetyl-L-Arg
0.057 - 11.28
acetyl-L-Arg methyl ester
10.7
benzoyl-Arg ethyl ester
-
recombinant enzyme
10.8
benzoyl-arginine ethyl ester
-
native enzyme
0.52 - 14.43
benzoyl-L-Arg
0.057 - 15.24
benzoyl-L-Arg ethyl ester
5.69 - 5.74
benzoyl-L-Arg methyl ester
5.31 - 5.51
benzoyl-L-Arg-NH2
3.35
benzoyl-L-arginine
37°C
2.76 - 6
benzoyl-L-arginine amide
5.94
benzoyl-L-arginine ethyl ester
37°C
5.57
benzoyl-L-arginine methyl ester
37°C
2
KDRNW
-
in 60 mM MOPS buffer, pH 7.5 and 10 mM CaCl2, at 37°C
0.17 - 0.31
L-Arg methyl ester
2.9
LDRGE
-
in 60 mM MOPS buffer, pH 7.5 and 10 mM CaCl2, at 37°C
2
MWRHV
-
in 60 mM MOPS buffer, pH 7.5 and 10 mM CaCl2, at 37°C
1.85 - 3.57
N-alpha-benzoyl-L-arginine amide
0.03 - 11.7
N-alpha-benzoyl-L-arginine ethyl ester
1.26 - 5.57
N-alpha-benzoyl-L-arginine methyl ester
0.55 - 3.8
Nalpha-benzoyl L-arginine ethyl ester
0.63
Nalpha-benzoyl-L-arginine
at pH 7.6 and 37°C
0.32
Nalpha-benzoyl-L-arginine amide
at pH 7.6 and 37°C
3.2 - 13.9
Nalpha-benzoyl-L-arginine ethyl ester
0.43
Nalpha-benzoyl-L-arginine methyl ester
at pH 7.6 and 37°C
0.7
TKQTARKSTGGK
-
in 60 mM MOPS buffer, pH 7.5 and 10 mM CaCl2, at 37°C
1.3
TSTGGRQGSHH
-
in 60 mM MOPS buffer, pH 7.5 and 10 mM CaCl2, at 37°C
1.8
WSRYH
-
in 60 mM MOPS buffer, pH 7.5 and 10 mM CaCl2, at 37°C
3.3
WTRGE
-
in 60 mM MOPS buffer, pH 7.5 and 10 mM CaCl2, at 37°C
2.3
YWRDH
-
in 60 mM MOPS buffer, pH 7.5 and 10 mM CaCl2, at 37°C
0.49 - 2.1
[histone H4]-L-Arg
0.21
Ac-Ala-Gly-Arg-Gly-Lys
-
isozyme PAD3, at 37°C, 10 mM CaCl2, 50 mM NaCl, 100 mM Tris-HCl, pH 7.6, 2 mM dithiothreitol
0.59
Ac-Ala-Gly-Arg-Gly-Lys
-
isozyme PAD1, at 37°C, 10 mM CaCl2, 50 mM NaCl, 100 mM Tris-HCl, pH 7.6, 2 mM dithiothreitol
0.62
Ac-Ser-Ala-Arg-Gly-Lys
-
isozyme PAD3, at 37°C, 10 mM CaCl2, 50 mM NaCl, 100 mM Tris-HCl, pH 7.6, 2 mM dithiothreitol
1.13
Ac-Ser-Ala-Arg-Gly-Lys
-
isozyme PAD1, at 37°C, 10 mM CaCl2, 50 mM NaCl, 100 mM Tris-HCl, pH 7.6, 2 mM dithiothreitol
0.57
Ac-Ser-Gly-Arg-Gly-acetyl-Lys-Gly-Gly-acetyl-Lys-Gly-Leu-Gly-acetyl-Lys-Gly-Gly-Ala-acetyl-Lys
-
isozyme PAD1, at 37°C, 10 mM CaCl2, 50 mM NaCl, 100 mM Tris-HCl, pH 7.6, 2 mM dithiothreitol
1.08
Ac-Ser-Gly-Arg-Gly-acetyl-Lys-Gly-Gly-acetyl-Lys-Gly-Leu-Gly-acetyl-Lys-Gly-Gly-Ala-acetyl-Lys
-
isozyme PAD3, at 37°C, 10 mM CaCl2, 50 mM NaCl, 100 mM Tris-HCl, pH 7.6, 2 mM dithiothreitol
0.75
Ac-Ser-Gly-Arg-Gly-Lys-Gly-Gly-Lys-Gly-Leu-Gly-Lys-Gly-Gly-Ala
-
isozyme PAD1, at 37°C, 10 mM CaCl2, 50 mM NaCl, 100 mM Tris-HCl, pH 7.6, 2 mM dithiothreitol
2.53
Ac-Ser-Gly-Arg-Gly-Lys-Gly-Gly-Lys-Gly-Leu-Gly-Lys-Gly-Gly-Ala
-
isozyme PAD4, at 37°C, 10 mM CaCl2, 50 mM NaCl, 100 mM Tris-HCl, pH 7.6, 2 mM dithiothreitol
0.08
Ac-Ser-Gly-Arg-Gly-Lys-Gly-Gly-Lys-Gly-Leu-Gly-Lys-Gly-Gly-Ala-Lys
-
isozyme PAD3, at 37°C, 10 mM CaCl2, 50 mM NaCl, 100 mM Tris-HCl, pH 7.6, 2 mM dithiothreitol
0.6
Ac-Ser-Gly-Arg-Gly-Lys-Gly-Gly-Lys-Gly-Leu-Gly-Lys-Gly-Gly-Ala-Lys
-
isozyme PAD1, at 37°C, 10 mM CaCl2, 50 mM NaCl, 100 mM Tris-HCl, pH 7.6, 2 mM dithiothreitol
2.3
Ac-Ser-Gly-Arg-Gly-Lys-Gly-Gly-Lys-Gly-Leu-Gly-Lys-Gly-Gly-Ala-Lys
-
isozyme PAD4, at 37°C, 10 mM CaCl2, 50 mM NaCl, 100 mM Tris-HCl, pH 7.6, 2 mM dithiothreitol
0.64
Ac-Ser-Gly-Arg-Gly-Lys-Gly-Gly-Lys-Gly-Leu-Gly-Lys-Gly-Gly-Ala-Lys-Arg-His
-
isozyme PAD1, at 37°C, 10 mM CaCl2, 50 mM NaCl, 100 mM Tris-HCl, pH 7.6, 2 mM dithiothreitol
2.29
Ac-Ser-Gly-Arg-Gly-Lys-Gly-Gly-Lys-Gly-Leu-Gly-Lys-Gly-Gly-Ala-Lys-Arg-His
-
isozyme PAD4, at 37°C, 10 mM CaCl2, 50 mM NaCl, 100 mM Tris-HCl, pH 7.6, 2 mM dithiothreitol
4.92
Ac-Ser-Gly-Arg-Gly-Lys-Gly-Gly-Lys-Gly-Leu-Gly-Lys-Gly-Gly-Ala-Lys-Arg-His
-
isozyme PAD3, at 37°C, 10 mM CaCl2, 50 mM NaCl, 100 mM Tris-HCl, pH 7.6, 2 mM dithiothreitol
0.31
Ac-Ser-Gly-Arg-Gly-Lys-Gly-Gly-Lys-Gly-Leu-Gly-Lys-Gly-Gly-Ala-Lys-Arg-His-Arg-Lys-Val
-
isozyme PAD3, at 37°C, 10 mM CaCl2, 50 mM NaCl, 100 mM Tris-HCl, pH 7.6, 2 mM dithiothreitol
0.81
Ac-Ser-Gly-Arg-Gly-Lys-Gly-Gly-Lys-Gly-Leu-Gly-Lys-Gly-Gly-Ala-Lys-Arg-His-Arg-Lys-Val
-
isozyme PAD1, at 37°C, 10 mM CaCl2, 50 mM NaCl, 100 mM Tris-HCl, pH 7.6, 2 mM dithiothreitol
3.39
Ac-Ser-Gly-Arg-Gly-Lys-Gly-Gly-Lys-Gly-Leu-Gly-Lys-Gly-Gly-Ala-Lys-Arg-His-Arg-Lys-Val
-
isozyme PAD4, at 37°C, 10 mM CaCl2, 50 mM NaCl, 100 mM Tris-HCl, pH 7.6, 2 mM dithiothreitol
0.34
Ac-Ser-Gly-Arg-Gly-Lys-Gly-Gly-Lys-Gly-Leu-Gly-Lys-Gly-Gly-Ala-Lys-Lys-His-Lys-Ala-Val
-
isozyme PAD3, at 37°C, 10 mM CaCl2, 50 mM NaCl, 100 mM Tris-HCl, pH 7.6, 2 mM dithiothreitol
0.56
Ac-Ser-Gly-Arg-Gly-Lys-Gly-Gly-Lys-Gly-Leu-Gly-Lys-Gly-Gly-Ala-Lys-Lys-His-Lys-Ala-Val
-
isozyme PAD1, at 37°C, 10 mM CaCl2, 50 mM NaCl, 100 mM Tris-HCl, pH 7.6, 2 mM dithiothreitol
0.32
acetyl-L-Arg
-
recombinant enzyme
0.37
acetyl-L-Arg
-
native enzyme
13.68
acetyl-L-Arg
pH 7.6, 55°C, peptidylarginine deiminase 1
0.057 - 0.65
acetyl-L-Arg methyl ester
pH 7.6, 55°C, peptidylarginine deiminase 1
1.93
acetyl-L-Arg methyl ester
pH 7.6, 55°C, peptidylarginine deiminase 3
4.11
acetyl-L-Arg methyl ester
-
native enzyme
4.41
acetyl-L-Arg methyl ester
-
recombinant enzyme
6.22
acetyl-L-Arg methyl ester
pH 7.6, 55°C, peptidylarginine deiminase 2
11.28
acetyl-L-Arg methyl ester
pH 7.6, 55°C, peptidylarginine deiminase 1
0.52
benzoyl-L-Arg
pH 7.6, 55°C, peptidylarginine deiminase 1
0.79
benzoyl-L-Arg
pH 7.6, 55°C, peptidylarginine deiminase 3
1.11
benzoyl-L-Arg
-
native enzyme
1.41
benzoyl-L-Arg
-
recombinant enzyme
3.15
benzoyl-L-Arg
pH 7.6, 55°C, peptidylarginine deiminase 2
14.43
benzoyl-L-Arg
pH 7.6, 55°C, peptidylarginine deiminase 1
0.057 - 0.65
benzoyl-L-Arg ethyl ester
pH 7.6, 55°C, peptidylarginine deiminase 1
0.55
benzoyl-L-Arg ethyl ester
pH 7.6, 55°C, peptidylarginine deiminase 2
1.73
benzoyl-L-Arg ethyl ester
pH 7.6, 55°C, peptidylarginine deiminase 3
11.31
benzoyl-L-Arg ethyl ester
pH 7.6, 55°C, peptidylarginine deiminase 1
15.24
benzoyl-L-Arg ethyl ester
pH 7.6, 55°C, peptidylarginine deiminase 2
5.69
benzoyl-L-Arg methyl ester
-
recombinant enzyme
5.74
benzoyl-L-Arg methyl ester
-
native enzyme
5.31
benzoyl-L-Arg-NH2
-
recombinant enzyme
5.51
benzoyl-L-Arg-NH2
-
native enzyme
2.76
benzoyl-L-arginine amide
37°C
3 - 6
benzoyl-L-arginine amide
37°C
2.88
IHAREIFDSR
pH 7.5, 37°C
7
IHAREIFDSR
pH 7.5, 37°C
0.22
L-Arg
-
recombinant enzyme
0.52
L-Arg
-
native enzyme
0.17
L-Arg methyl ester
-
recombinant enzyme
0.31
L-Arg methyl ester
-
native enzyme
1.85
N-alpha-benzoyl-L-arginine amide
-
isozyme PAD3, at 37°C, 10 mM CaCl2, 50 mM NaCl, 100 mM Tris-HCl, pH 7.6, 2 mM dithiothreitol
2.1
N-alpha-benzoyl-L-arginine amide
-
mutant PAD3 enzyme G374R, at 37°C, 10 mM CaCl2, 50 mM NaCl, 100 mM Tris-HCl, pH 7.6, 2 mM dithiothreitol
2.76
N-alpha-benzoyl-L-arginine amide
-
isozyme PAD4, at 37°C, 10 mM CaCl2, 50 mM NaCl, 100 mM Tris-HCl, pH 7.6, 2 mM dithiothreitol
3.57
N-alpha-benzoyl-L-arginine amide
-
isozyme PAD1, at 37°C, 10 mM CaCl2, 50 mM NaCl, 100 mM Tris-HCl, pH 7.6, 2 mM dithiothreitol
0.03
N-alpha-benzoyl-L-arginine ethyl ester
mutant Q350A, pH 7.6, 37°C
0.03
N-alpha-benzoyl-L-arginine ethyl ester
pH 7.6, 37°C, mutant enzyme Q350A
0.04
N-alpha-benzoyl-L-arginine ethyl ester
mutant V469T, pH 7.5, 25°C
0.07
N-alpha-benzoyl-L-arginine ethyl ester
mutant R372A, pH 7.5, 25°C
0.1
N-alpha-benzoyl-L-arginine ethyl ester
mutant E412A, pH 7.6, 37°C
0.1
N-alpha-benzoyl-L-arginine ethyl ester
mutant V469L, pH 7.5, 25°C
0.1
N-alpha-benzoyl-L-arginine ethyl ester
pH 7.6, 37°C, mutant enzyme E412A
0.12
N-alpha-benzoyl-L-arginine ethyl ester
mutant R372Q, pH 7.5, 25°C
0.2
N-alpha-benzoyl-L-arginine ethyl ester
mutant R373A, pH 7.6, 37°C
0.2
N-alpha-benzoyl-L-arginine ethyl ester
pH 7.6, 37°C, mutant enzyme W373A
0.25
N-alpha-benzoyl-L-arginine ethyl ester
mutant E352A, pH 7.6, 37°C
0.25
N-alpha-benzoyl-L-arginine ethyl ester
pH 7.6, 37°C, mutant enzyme E352A
0.27
N-alpha-benzoyl-L-arginine ethyl ester
-
isozyme PAD1, at 37°C, 10 mM CaCl2, 50 mM NaCl, 100 mM Tris-HCl, pH 7.6, 2 mM dithiothreitol
0.35
N-alpha-benzoyl-L-arginine ethyl ester
mutant D166A, pH 7.6, 37°C
0.35
N-alpha-benzoyl-L-arginine ethyl ester
pH 7.6, 37°C, mutant enzyme D166A
0.51
N-alpha-benzoyl-L-arginine ethyl ester
mutant D389A, pH 7.6, 37°C
0.51
N-alpha-benzoyl-L-arginine ethyl ester
pH 7.6, 37°C, mutant enzyme D389A
0.6
N-alpha-benzoyl-L-arginine ethyl ester
mutant D370A, pH 7.6, 37°C
0.6
N-alpha-benzoyl-L-arginine ethyl ester
mutant W548K, pH 7.5, 25°C
0.6
N-alpha-benzoyl-L-arginine ethyl ester
pH 7.6, 37°C, mutant enzyme D370A
0.8
N-alpha-benzoyl-L-arginine ethyl ester
mutant D123N, pH 7.6, 37°C
0.8
N-alpha-benzoyl-L-arginine ethyl ester
mutant D125A, pH 7.6, 37°C
0.8
N-alpha-benzoyl-L-arginine ethyl ester
mutant V469A, pH 7.5, 25°C
0.8
N-alpha-benzoyl-L-arginine ethyl ester
pH 7.6, 37°C, mutant enzyme D123N
0.8
N-alpha-benzoyl-L-arginine ethyl ester
pH 7.6, 37°C, mutant enzyme D125A
1.53
N-alpha-benzoyl-L-arginine ethyl ester
mutant R347A, pH 7.6, 37°C
1.53
N-alpha-benzoyl-L-arginine ethyl ester
pH 7.6, 37°C, mutant enzyme R347
1.55
N-alpha-benzoyl-L-arginine ethyl ester
mutant D374A, pH 7.6, 37°C
1.55
N-alpha-benzoyl-L-arginine ethyl ester
pH 7.6, 37°C, mutant enzyme D374A
1.8
N-alpha-benzoyl-L-arginine ethyl ester
mutant D169A, pH 7.6, 37°C
1.8
N-alpha-benzoyl-L-arginine ethyl ester
mutant F221A/F222A, pH 7.6, 37°C
1.8
N-alpha-benzoyl-L-arginine ethyl ester
pH 7.6, 37°C, mutant enzyme D169A
1.8
N-alpha-benzoyl-L-arginine ethyl ester
pH 7.6, 37°C, mutant enzyme F221A/F222A
2.3
N-alpha-benzoyl-L-arginine ethyl ester
mutant R441A, pH 7.5, 25°C
2.65
N-alpha-benzoyl-L-arginine ethyl ester
pH 7.6, 37°C, wild-type enzyme
2.65
N-alpha-benzoyl-L-arginine ethyl ester
wild-type, pH 7.6, 37°C
2.65
N-alpha-benzoyl-L-arginine ethyl ester
mutant D177A, pH 7.6, 37°C
2.65
N-alpha-benzoyl-L-arginine ethyl ester
pH 7.6, 37°C, mutant enzyme D177A
2.8
N-alpha-benzoyl-L-arginine ethyl ester
mutant Y435A, pH 7.5, 25°C
2.9
N-alpha-benzoyl-L-arginine ethyl ester
mutant V284D, pH 7.5, 25°C
3.5
N-alpha-benzoyl-L-arginine ethyl ester
mutant D465A, pH 7.5, 25°C
3.6
N-alpha-benzoyl-L-arginine ethyl ester
mutant L279D, pH 7.5, 25°C
3.7
N-alpha-benzoyl-L-arginine ethyl ester
mutant R374Q, pH 7.5, 25°C
4.5
N-alpha-benzoyl-L-arginine ethyl ester
mutant L6D, pH 7.5, 25°C
5
N-alpha-benzoyl-L-arginine ethyl ester
mutant R639A, pH 7.5, 25°C
5.5
N-alpha-benzoyl-L-arginine ethyl ester
mutant L279A, pH 7.5, 25°C
5.5
N-alpha-benzoyl-L-arginine ethyl ester
mutant V283T, pH 7.5, 25°C
5.5
N-alpha-benzoyl-L-arginine ethyl ester
mutant V284A, pH 7.5, 25°C
5.94
N-alpha-benzoyl-L-arginine ethyl ester
-
isozyme PAD4, at 37°C, 10 mM CaCl2, 50 mM NaCl, 100 mM Tris-HCl, pH 7.6, 2 mM dithiothreitol
6.2
N-alpha-benzoyl-L-arginine ethyl ester
mutant W548F, pH 7.5, 25°C
6.9
N-alpha-benzoyl-L-arginine ethyl ester
mutant F285A, pH 7.5, 25°C
7.3
N-alpha-benzoyl-L-arginine ethyl ester
mutant L6A, pH 7.5, 25°C
7.7
N-alpha-benzoyl-L-arginine ethyl ester
mutant F541A, pH 7.5, 25°C
7.8
N-alpha-benzoyl-L-arginine ethyl ester
mutant R374A, pH 7.5, 25°C
8
N-alpha-benzoyl-L-arginine ethyl ester
mutant C434A, pH 7.5, 25°C
8
N-alpha-benzoyl-L-arginine ethyl ester
mutant V283A, pH 7.5, 25°C
9.5
N-alpha-benzoyl-L-arginine ethyl ester
mutant V283I, pH 7.5, 25°C
9.8
N-alpha-benzoyl-L-arginine ethyl ester
mutant F576A, pH 7.5, 25°C
10.9
N-alpha-benzoyl-L-arginine ethyl ester
mutant L6I, pH 7.5, 25°C
10.9
N-alpha-benzoyl-L-arginine ethyl ester
mutant V284I, pH 7.5, 25°C
11.2
N-alpha-benzoyl-L-arginine ethyl ester
mutant L279I, pH 7.5, 25°C
11.7
N-alpha-benzoyl-L-arginine ethyl ester
wild-type, pH 7.5, 25°C
1.26
N-alpha-benzoyl-L-arginine methyl ester
-
isozyme PAD3, at 37°C, 10 mM CaCl2, 50 mM NaCl, 100 mM Tris-HCl, pH 7.6, 2 mM dithiothreitol
3.85
N-alpha-benzoyl-L-arginine methyl ester
-
isozyme PAD1, at 37°C, 10 mM CaCl2, 50 mM NaCl, 100 mM Tris-HCl, pH 7.6, 2 mM dithiothreitol
5.57
N-alpha-benzoyl-L-arginine methyl ester
-
isozyme PAD4, at 37°C, 10 mM CaCl2, 50 mM NaCl, 100 mM Tris-HCl, pH 7.6, 2 mM dithiothreitol
0.55
Nalpha-benzoyl L-arginine ethyl ester
-
37°C, pH 7.6, mutant R419Q
0.76
Nalpha-benzoyl L-arginine ethyl ester
-
37°C, pH 7.6, mutant R156K
1.1
Nalpha-benzoyl L-arginine ethyl ester
-
37°C, pH 7.6, mutant R484K
1.2
Nalpha-benzoyl L-arginine ethyl ester
-
37°C, pH 7.6, mutant R205Q
1.2
Nalpha-benzoyl L-arginine ethyl ester
-
37°C, pH 7.6, mutant R484Q
1.5
Nalpha-benzoyl L-arginine ethyl ester
-
37°C, pH 7.6, mutant A112G
1.6
Nalpha-benzoyl L-arginine ethyl ester
-
37°C, pH 7.6, mutant R639Q
1.9
Nalpha-benzoyl L-arginine ethyl ester
-
37°C, pH 7.6, mutant R609Q
1.9
Nalpha-benzoyl L-arginine ethyl ester
-
37°C, pH 7.6, mutant R639K
2.3
Nalpha-benzoyl L-arginine ethyl ester
-
37°C, pH 7.6, mutant R123Q
2.3
Nalpha-benzoyl L-arginine ethyl ester
-
37°C, pH 7.6, mutant R374Q
2.6
Nalpha-benzoyl L-arginine ethyl ester
-
37°C, pH 7.6, mutant R609K
2.7
Nalpha-benzoyl L-arginine ethyl ester
-
37°C, pH 7.6, mutant A82V
2.8
Nalpha-benzoyl L-arginine ethyl ester
-
37°C, pH 7.6, mutant S55G
2.8
Nalpha-benzoyl L-arginine ethyl ester
-
37°C, pH 7.6, wild-type
3
Nalpha-benzoyl L-arginine ethyl ester
-
37°C, pH 7.6, mutant R374K
3.1
Nalpha-benzoyl L-arginine ethyl ester
-
37°C, pH 7.6, mutant R419K
3.6
Nalpha-benzoyl L-arginine ethyl ester
-
37°C, pH 7.6, mutant R156Q
3.7
Nalpha-benzoyl L-arginine ethyl ester
-
37°C, pH 7.6, mutant R123K
3.8
Nalpha-benzoyl L-arginine ethyl ester
-
37°C, pH 7.6, mutant R205K
3.2
Nalpha-benzoyl-L-arginine ethyl ester
at pH 7.6 and 37°C
3.3
Nalpha-benzoyl-L-arginine ethyl ester
-
mutant R8E/D547E, pH 7.4, 25°C
4
Nalpha-benzoyl-L-arginine ethyl ester
-
mutant Y435N, pH 7.4, 25°C
5.3
Nalpha-benzoyl-L-arginine ethyl ester
-
mutant R8L, pH 7.4, 25°C
5.9
Nalpha-benzoyl-L-arginine ethyl ester
-
mutant Y435A, pH 7.4, 25°C
7.3
Nalpha-benzoyl-L-arginine ethyl ester
-
mutant R8E, pH 7.4, 25°C
8.9
Nalpha-benzoyl-L-arginine ethyl ester
-
mutant D547E, pH 7.4, 25°C
10.08
Nalpha-benzoyl-L-arginine ethyl ester
-
mutant R8K, pH 7.4, 25°C
10.7
Nalpha-benzoyl-L-arginine ethyl ester
-
mutant R8A/D547A, pH 7.4, 25°C
11.6
Nalpha-benzoyl-L-arginine ethyl ester
-
mutant D547A, pH 7.4, 25°C
12.1
Nalpha-benzoyl-L-arginine ethyl ester
-
mutant E281A, pH 7.4, 25°C
12.2
Nalpha-benzoyl-L-arginine ethyl ester
-
mutant D547N, pH 7.4, 25°C
12.2
Nalpha-benzoyl-L-arginine ethyl ester
-
mutant R8A, pH 7.4, 25°C
12.2
Nalpha-benzoyl-L-arginine ethyl ester
-
mutant R8Q , pH 7.4, 25°C
13.2
Nalpha-benzoyl-L-arginine ethyl ester
-
mutant Y237A/E281A, pH 7.4, 25°C
13.3
Nalpha-benzoyl-L-arginine ethyl ester
-
mutant R8H, pH 7.4, 25°C
13.4
Nalpha-benzoyl-L-arginine ethyl ester
-
wild-type, pH 7.4, 25°C
13.5
Nalpha-benzoyl-L-arginine ethyl ester
-
mutant D273A/R544A, pH 7.4, 25°C
13.9
Nalpha-benzoyl-L-arginine ethyl ester
-
mutant Y237A, pH 7.4, 25°C
3.03
PPGFSPFR
pH 7.5, 37°C
11.3
PPGFSPFR
pH 7.5, 37°C
0.49
[histone H4]-L-Arg
-
37°C, pH 7.6, mutant R374K
0.77
[histone H4]-L-Arg
-
37°C, pH 7.6, mutant R374Q
0.78
[histone H4]-L-Arg
-
37°C, pH 7.6, mutant R639Q
0.79
[histone H4]-L-Arg
-
37°C, pH 7.6, mutant R156Q
0.9
[histone H4]-L-Arg
-
37°C, pH 7.6, mutant R123Q
1
[histone H4]-L-Arg
-
37°C, pH 7.6, mutant A112G
1.1
[histone H4]-L-Arg
-
37°C, pH 7.6, mutant S55G
1.2
[histone H4]-L-Arg
-
37°C, pH 7.6, mutant R419Q
1.3
[histone H4]-L-Arg
-
37°C, pH 7.6, mutant R419K
1.3
[histone H4]-L-Arg
-
37°C, pH 7.6, mutant R639K
1.4
[histone H4]-L-Arg
-
37°C, pH 7.6, mutant A82V
1.4
[histone H4]-L-Arg
-
37°C, pH 7.6, mutant R156K
1.5
[histone H4]-L-Arg
-
37°C, pH 7.6, mutant R609Q
1.6
[histone H4]-L-Arg
-
37°C, pH 7.6, mutant R484K
1.6
[histone H4]-L-Arg
-
37°C, pH 7.6, mutant R484Q
1.7
[histone H4]-L-Arg
-
37°C, pH 7.6, mutant R123K
2.1
[histone H4]-L-Arg
-
37°C, pH 7.6, wild-type
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
0.2 - 5.7
2-acetyl-Gly-Arg-Gly-Lys-Gly-Gly-Lys-Gly-Leu-Gly-Lys-Gly-Gly-Ala-Lys
0.001 - 1.4
3-acetyl-Arg-Gly-Lys-Gly-Gly-Lys-Gly-Leu-Gly-Lys-Gly-Gly-Ala-Lys
0.22 - 3.5
Ac-Ala-Gly-Arg-Gly-Lys
0.9 - 5.8
Ac-Ser-Ala-Arg-Gly-Lys
0.01 - 3.3
Ac-Ser-Gly-Ala-Gly-Lys-Gly-Gly-Lys-Gly-Leu-Gly-Lys-Gly-Gly-Ala-Ala-Arg-His-Arg-Lys-Val
0.08 - 6.6
Ac-Ser-Gly-Arg-Ala-Lys
1.8 - 3.9
Ac-Ser-Gly-Arg-Gly-acetyl-Lys-Gly-Gly-acetyl-Lys-Gly-Leu-Gly-acetyl-Lys-Gly-Gly-Ala-acetyl-Lys
0.05 - 16
Ac-Ser-Gly-Arg-Gly-Ala
0.001 - 3.3
Ac-Ser-Gly-Arg-Gly-Lys-Gly-Gly-Lys-Gly-Leu-Gly-Lys-Gly
2 - 4.6
Ac-Ser-Gly-Arg-Gly-Lys-Gly-Gly-Lys-Gly-Leu-Gly-Lys-Gly-Gly-Ala
1 - 19
Ac-Ser-Gly-Arg-Gly-Lys-Gly-Gly-Lys-Gly-Leu-Gly-Lys-Gly-Gly-Ala-Ala-Arg-His-Arg-Ala-Val
0.7 - 14
Ac-Ser-Gly-Arg-Gly-Lys-Gly-Gly-Lys-Gly-Leu-Gly-Lys-Gly-Gly-Ala-Ala-Arg-His-Arg-Lys-Val
0.5 - 3.9
Ac-Ser-Gly-Arg-Gly-Lys-Gly-Gly-Lys-Gly-Leu-Gly-Lys-Gly-Gly-Ala-Lys
0.3 - 2.7
Ac-Ser-Gly-Arg-Gly-Lys-Gly-Gly-Lys-Gly-Leu-Gly-Lys-Gly-Gly-Ala-Lys-Ala-His-Arg-Ala-Val
2.5 - 4.4
Ac-Ser-Gly-Arg-Gly-Lys-Gly-Gly-Lys-Gly-Leu-Gly-Lys-Gly-Gly-Ala-Lys-Arg-His
0.001 - 3.7
Ac-Ser-Gly-Arg-Gly-Lys-Gly-Gly-Lys-Gly-Leu-Gly-Lys-Gly-Gly-Ala-Lys-Arg-His-Ala-Ala-Val
0.7 - 5.3
Ac-Ser-Gly-Arg-Gly-Lys-Gly-Gly-Lys-Gly-Leu-Gly-Lys-Gly-Gly-Ala-Lys-Arg-His-Arg-Lys-Val
0.4 - 1.2
Ac-Ser-Gly-Arg-Gly-Lys-Gly-Gly-Lys-Gly-Leu-Gly-Lys-Gly-Gly-Ala-Lys-Lys-His-Lys-Ala-Val
0.7 - 9
histone H4-L-arginine
0.13 - 120000
N-alpha-benzoyl-L-arginine amide
0.005 - 13800
N-alpha-benzoyl-L-arginine ethyl ester
0.12 - 10.4
N-alpha-benzoyl-L-arginine methyl ester
0.63 - 6.4
Nalpha-benzoyl L-arginine ethyl ester
0.39
Nalpha-benzoyl-L-arginine
at pH 7.6 and 37°C
0.68
Nalpha-benzoyl-L-arginine amide
at pH 7.6 and 37°C
11.7
Nalpha-benzoyl-L-arginine ethyl ester
at pH 7.6 and 37°C
1.8
Nalpha-benzoyl-L-arginine methyl ester
at pH 7.6 and 37°C
0.0031 - 10
[histone H4]-L-Arg
0.2
2-acetyl-Gly-Arg-Gly-Lys-Gly-Gly-Lys-Gly-Leu-Gly-Lys-Gly-Gly-Ala-Lys
-
isozyme PAD3, at 37°C, 10 mM CaCl2, 50 mM NaCl, 100 mM Tris-HCl, pH 7.6, 2 mM dithiothreitol
4.2
2-acetyl-Gly-Arg-Gly-Lys-Gly-Gly-Lys-Gly-Leu-Gly-Lys-Gly-Gly-Ala-Lys
-
isozyme PAD1, at 37°C, 10 mM CaCl2, 50 mM NaCl, 100 mM Tris-HCl, pH 7.6, 2 mM dithiothreitol
5.7
2-acetyl-Gly-Arg-Gly-Lys-Gly-Gly-Lys-Gly-Leu-Gly-Lys-Gly-Gly-Ala-Lys
-
isozyme PAD4, at 37°C, 10 mM CaCl2, 50 mM NaCl, 100 mM Tris-HCl, pH 7.6, 2 mM dithiothreitol
0.001
3-acetyl-Arg-Gly-Lys-Gly-Gly-Lys-Gly-Leu-Gly-Lys-Gly-Gly-Ala-Lys
-
kcat_Km value less than 0.001 mM-1*sec-1, isozyme PAD3, at 37°C, 10 mM CaCl2, 50 mM NaCl, 100 mM Tris-HCl, pH 7.6, 2 mM dithiothreitol
1.4
3-acetyl-Arg-Gly-Lys-Gly-Gly-Lys-Gly-Leu-Gly-Lys-Gly-Gly-Ala-Lys
-
isozyme PAD1, at 37°C, 10 mM CaCl2, 50 mM NaCl, 100 mM Tris-HCl, pH 7.6, 2 mM dithiothreitol
1.4
3-acetyl-Arg-Gly-Lys-Gly-Gly-Lys-Gly-Leu-Gly-Lys-Gly-Gly-Ala-Lys
-
isozyme PAD4, at 37°C, 10 mM CaCl2, 50 mM NaCl, 100 mM Tris-HCl, pH 7.6, 2 mM dithiothreitol
0.22
Ac-Ala-Gly-Arg-Gly-Lys
-
isozyme PAD3, at 37°C, 10 mM CaCl2, 50 mM NaCl, 100 mM Tris-HCl, pH 7.6, 2 mM dithiothreitol
2
Ac-Ala-Gly-Arg-Gly-Lys
-
isozyme PAD1, at 37°C, 10 mM CaCl2, 50 mM NaCl, 100 mM Tris-HCl, pH 7.6, 2 mM dithiothreitol
3.5
Ac-Ala-Gly-Arg-Gly-Lys
-
isozyme PAD4, at 37°C, 10 mM CaCl2, 50 mM NaCl, 100 mM Tris-HCl, pH 7.6, 2 mM dithiothreitol
0.9
Ac-Ser-Ala-Arg-Gly-Lys
-
isozyme PAD3, at 37°C, 10 mM CaCl2, 50 mM NaCl, 100 mM Tris-HCl, pH 7.6, 2 mM dithiothreitol
1.4
Ac-Ser-Ala-Arg-Gly-Lys
-
isozyme PAD1, at 37°C, 10 mM CaCl2, 50 mM NaCl, 100 mM Tris-HCl, pH 7.6, 2 mM dithiothreitol
5.8
Ac-Ser-Ala-Arg-Gly-Lys
-
isozyme PAD4, at 37°C, 10 mM CaCl2, 50 mM NaCl, 100 mM Tris-HCl, pH 7.6, 2 mM dithiothreitol
0.01
Ac-Ser-Gly-Ala-Gly-Lys-Gly-Gly-Lys-Gly-Leu-Gly-Lys-Gly-Gly-Ala-Ala-Arg-His-Arg-Lys-Val
-
isozyme PAD3, at 37°C, 10 mM CaCl2, 50 mM NaCl, 100 mM Tris-HCl, pH 7.6, 2 mM dithiothreitol
0.58
Ac-Ser-Gly-Ala-Gly-Lys-Gly-Gly-Lys-Gly-Leu-Gly-Lys-Gly-Gly-Ala-Ala-Arg-His-Arg-Lys-Val
-
isozyme PAD4, at 37°C, 10 mM CaCl2, 50 mM NaCl, 100 mM Tris-HCl, pH 7.6, 2 mM dithiothreitol
3.3
Ac-Ser-Gly-Ala-Gly-Lys-Gly-Gly-Lys-Gly-Leu-Gly-Lys-Gly-Gly-Ala-Ala-Arg-His-Arg-Lys-Val
-
isozyme PAD1, at 37°C, 10 mM CaCl2, 50 mM NaCl, 100 mM Tris-HCl, pH 7.6, 2 mM dithiothreitol
0.08
Ac-Ser-Gly-Arg-Ala-Lys
-
isozyme PAD3, at 37°C, 10 mM CaCl2, 50 mM NaCl, 100 mM Tris-HCl, pH 7.6, 2 mM dithiothreitol
1.7
Ac-Ser-Gly-Arg-Ala-Lys
-
isozyme PAD1, at 37°C, 10 mM CaCl2, 50 mM NaCl, 100 mM Tris-HCl, pH 7.6, 2 mM dithiothreitol
6.6
Ac-Ser-Gly-Arg-Ala-Lys
-
isozyme PAD4, at 37°C, 10 mM CaCl2, 50 mM NaCl, 100 mM Tris-HCl, pH 7.6, 2 mM dithiothreitol
1.8
Ac-Ser-Gly-Arg-Gly-acetyl-Lys-Gly-Gly-acetyl-Lys-Gly-Leu-Gly-acetyl-Lys-Gly-Gly-Ala-acetyl-Lys
-
isozyme PAD3, at 37°C, 10 mM CaCl2, 50 mM NaCl, 100 mM Tris-HCl, pH 7.6, 2 mM dithiothreitol
3.4
Ac-Ser-Gly-Arg-Gly-acetyl-Lys-Gly-Gly-acetyl-Lys-Gly-Leu-Gly-acetyl-Lys-Gly-Gly-Ala-acetyl-Lys
-
isozyme PAD4, at 37°C, 10 mM CaCl2, 50 mM NaCl, 100 mM Tris-HCl, pH 7.6, 2 mM dithiothreitol
3.9
Ac-Ser-Gly-Arg-Gly-acetyl-Lys-Gly-Gly-acetyl-Lys-Gly-Leu-Gly-acetyl-Lys-Gly-Gly-Ala-acetyl-Lys
-
isozyme PAD1, at 37°C, 10 mM CaCl2, 50 mM NaCl, 100 mM Tris-HCl, pH 7.6, 2 mM dithiothreitol
0.05
Ac-Ser-Gly-Arg-Gly-Ala
-
isozyme PAD3, at 37°C, 10 mM CaCl2, 50 mM NaCl, 100 mM Tris-HCl, pH 7.6, 2 mM dithiothreitol
2.5
Ac-Ser-Gly-Arg-Gly-Ala
-
isozyme PAD1, at 37°C, 10 mM CaCl2, 50 mM NaCl, 100 mM Tris-HCl, pH 7.6, 2 mM dithiothreitol
16
Ac-Ser-Gly-Arg-Gly-Ala
-
isozyme PAD4, at 37°C, 10 mM CaCl2, 50 mM NaCl, 100 mM Tris-HCl, pH 7.6, 2 mM dithiothreitol
0.001
Ac-Ser-Gly-Arg-Gly-Lys-Gly-Gly-Lys-Gly-Leu-Gly-Lys-Gly
-
kcat_Km value less than 0.001 mM-1*sec-1, isozyme PAD1, at 37°C, 10 mM CaCl2, 50 mM NaCl, 100 mM Tris-HCl, pH 7.6, 2 mM dithiothreitol
0.085
Ac-Ser-Gly-Arg-Gly-Lys-Gly-Gly-Lys-Gly-Leu-Gly-Lys-Gly
-
isozyme PAD3, at 37°C, 10 mM CaCl2, 50 mM NaCl, 100 mM Tris-HCl, pH 7.6, 2 mM dithiothreitol
3.3
Ac-Ser-Gly-Arg-Gly-Lys-Gly-Gly-Lys-Gly-Leu-Gly-Lys-Gly
-
isozyme PAD4, at 37°C, 10 mM CaCl2, 50 mM NaCl, 100 mM Tris-HCl, pH 7.6, 2 mM dithiothreitol
2
Ac-Ser-Gly-Arg-Gly-Lys-Gly-Gly-Lys-Gly-Leu-Gly-Lys-Gly-Gly-Ala
-
isozyme PAD3, at 37°C, 10 mM CaCl2, 50 mM NaCl, 100 mM Tris-HCl, pH 7.6, 2 mM dithiothreitol
4.4
Ac-Ser-Gly-Arg-Gly-Lys-Gly-Gly-Lys-Gly-Leu-Gly-Lys-Gly-Gly-Ala
-
isozyme PAD4, at 37°C, 10 mM CaCl2, 50 mM NaCl, 100 mM Tris-HCl, pH 7.6, 2 mM dithiothreitol
4.6
Ac-Ser-Gly-Arg-Gly-Lys-Gly-Gly-Lys-Gly-Leu-Gly-Lys-Gly-Gly-Ala
-
isozyme PAD1, at 37°C, 10 mM CaCl2, 50 mM NaCl, 100 mM Tris-HCl, pH 7.6, 2 mM dithiothreitol
1
Ac-Ser-Gly-Arg-Gly-Lys-Gly-Gly-Lys-Gly-Leu-Gly-Lys-Gly-Gly-Ala-Ala-Arg-His-Arg-Ala-Val
-
isozyme PAD3, at 37°C, 10 mM CaCl2, 50 mM NaCl, 100 mM Tris-HCl, pH 7.6, 2 mM dithiothreitol
2.3
Ac-Ser-Gly-Arg-Gly-Lys-Gly-Gly-Lys-Gly-Leu-Gly-Lys-Gly-Gly-Ala-Ala-Arg-His-Arg-Ala-Val
-
isozyme PAD1, at 37°C, 10 mM CaCl2, 50 mM NaCl, 100 mM Tris-HCl, pH 7.6, 2 mM dithiothreitol
19
Ac-Ser-Gly-Arg-Gly-Lys-Gly-Gly-Lys-Gly-Leu-Gly-Lys-Gly-Gly-Ala-Ala-Arg-His-Arg-Ala-Val
-
isozyme PAD4, at 37°C, 10 mM CaCl2, 50 mM NaCl, 100 mM Tris-HCl, pH 7.6, 2 mM dithiothreitol
0.7
Ac-Ser-Gly-Arg-Gly-Lys-Gly-Gly-Lys-Gly-Leu-Gly-Lys-Gly-Gly-Ala-Ala-Arg-His-Arg-Lys-Val
-
isozyme PAD3, at 37°C, 10 mM CaCl2, 50 mM NaCl, 100 mM Tris-HCl, pH 7.6, 2 mM dithiothreitol
1.3
Ac-Ser-Gly-Arg-Gly-Lys-Gly-Gly-Lys-Gly-Leu-Gly-Lys-Gly-Gly-Ala-Ala-Arg-His-Arg-Lys-Val
-
isozyme PAD1, at 37°C, 10 mM CaCl2, 50 mM NaCl, 100 mM Tris-HCl, pH 7.6, 2 mM dithiothreitol
14
Ac-Ser-Gly-Arg-Gly-Lys-Gly-Gly-Lys-Gly-Leu-Gly-Lys-Gly-Gly-Ala-Ala-Arg-His-Arg-Lys-Val
-
isozyme PAD4, at 37°C, 10 mM CaCl2, 50 mM NaCl, 100 mM Tris-HCl, pH 7.6, 2 mM dithiothreitol
0.5
Ac-Ser-Gly-Arg-Gly-Lys-Gly-Gly-Lys-Gly-Leu-Gly-Lys-Gly-Gly-Ala-Lys
-
isozyme PAD3, at 37°C, 10 mM CaCl2, 50 mM NaCl, 100 mM Tris-HCl, pH 7.6, 2 mM dithiothreitol
3.1
Ac-Ser-Gly-Arg-Gly-Lys-Gly-Gly-Lys-Gly-Leu-Gly-Lys-Gly-Gly-Ala-Lys
-
isozyme PAD1, at 37°C, 10 mM CaCl2, 50 mM NaCl, 100 mM Tris-HCl, pH 7.6, 2 mM dithiothreitol
3.9
Ac-Ser-Gly-Arg-Gly-Lys-Gly-Gly-Lys-Gly-Leu-Gly-Lys-Gly-Gly-Ala-Lys
-
isozyme PAD4, at 37°C, 10 mM CaCl2, 50 mM NaCl, 100 mM Tris-HCl, pH 7.6, 2 mM dithiothreitol
0.3
Ac-Ser-Gly-Arg-Gly-Lys-Gly-Gly-Lys-Gly-Leu-Gly-Lys-Gly-Gly-Ala-Lys-Ala-His-Arg-Ala-Val
-
isozyme PAD3, at 37°C, 10 mM CaCl2, 50 mM NaCl, 100 mM Tris-HCl, pH 7.6, 2 mM dithiothreitol
0.6
Ac-Ser-Gly-Arg-Gly-Lys-Gly-Gly-Lys-Gly-Leu-Gly-Lys-Gly-Gly-Ala-Lys-Ala-His-Arg-Ala-Val
-
isozyme PAD1, at 37°C, 10 mM CaCl2, 50 mM NaCl, 100 mM Tris-HCl, pH 7.6, 2 mM dithiothreitol
2.7
Ac-Ser-Gly-Arg-Gly-Lys-Gly-Gly-Lys-Gly-Leu-Gly-Lys-Gly-Gly-Ala-Lys-Ala-His-Arg-Ala-Val
-
isozyme PAD4, at 37°C, 10 mM CaCl2, 50 mM NaCl, 100 mM Tris-HCl, pH 7.6, 2 mM dithiothreitol
2.5
Ac-Ser-Gly-Arg-Gly-Lys-Gly-Gly-Lys-Gly-Leu-Gly-Lys-Gly-Gly-Ala-Lys-Arg-His
-
isozyme PAD3, at 37°C, 10 mM CaCl2, 50 mM NaCl, 100 mM Tris-HCl, pH 7.6, 2 mM dithiothreitol
3
Ac-Ser-Gly-Arg-Gly-Lys-Gly-Gly-Lys-Gly-Leu-Gly-Lys-Gly-Gly-Ala-Lys-Arg-His
-
isozyme PAD1, at 37°C, 10 mM CaCl2, 50 mM NaCl, 100 mM Tris-HCl, pH 7.6, 2 mM dithiothreitol
4.4
Ac-Ser-Gly-Arg-Gly-Lys-Gly-Gly-Lys-Gly-Leu-Gly-Lys-Gly-Gly-Ala-Lys-Arg-His
-
isozyme PAD4, at 37°C, 10 mM CaCl2, 50 mM NaCl, 100 mM Tris-HCl, pH 7.6, 2 mM dithiothreitol
0.001
Ac-Ser-Gly-Arg-Gly-Lys-Gly-Gly-Lys-Gly-Leu-Gly-Lys-Gly-Gly-Ala-Lys-Arg-His-Ala-Ala-Val
-
kcat_Km value less than 0.001 mM-1*sec-1, isozyme PAD3, at 37°C, 10 mM CaCl2, 50 mM NaCl, 100 mM Tris-HCl, pH 7.6, 2 mM dithiothreitol
0.4
Ac-Ser-Gly-Arg-Gly-Lys-Gly-Gly-Lys-Gly-Leu-Gly-Lys-Gly-Gly-Ala-Lys-Arg-His-Ala-Ala-Val
-
isozyme PAD4, at 37°C, 10 mM CaCl2, 50 mM NaCl, 100 mM Tris-HCl, pH 7.6, 2 mM dithiothreitol
3.7
Ac-Ser-Gly-Arg-Gly-Lys-Gly-Gly-Lys-Gly-Leu-Gly-Lys-Gly-Gly-Ala-Lys-Arg-His-Ala-Ala-Val
-
isozyme PAD1, at 37°C, 10 mM CaCl2, 50 mM NaCl, 100 mM Tris-HCl, pH 7.6, 2 mM dithiothreitol
0.7
Ac-Ser-Gly-Arg-Gly-Lys-Gly-Gly-Lys-Gly-Leu-Gly-Lys-Gly-Gly-Ala-Lys-Arg-His-Arg-Lys-Val
-
isozyme PAD3, at 37°C, 10 mM CaCl2, 50 mM NaCl, 100 mM Tris-HCl, pH 7.6, 2 mM dithiothreitol
4
Ac-Ser-Gly-Arg-Gly-Lys-Gly-Gly-Lys-Gly-Leu-Gly-Lys-Gly-Gly-Ala-Lys-Arg-His-Arg-Lys-Val
-
isozyme PAD1, at 37°C, 10 mM CaCl2, 50 mM NaCl, 100 mM Tris-HCl, pH 7.6, 2 mM dithiothreitol
5.3
Ac-Ser-Gly-Arg-Gly-Lys-Gly-Gly-Lys-Gly-Leu-Gly-Lys-Gly-Gly-Ala-Lys-Arg-His-Arg-Lys-Val
-
isozyme PAD4, at 37°C, 10 mM CaCl2, 50 mM NaCl, 100 mM Tris-HCl, pH 7.6, 2 mM dithiothreitol
0.4
Ac-Ser-Gly-Arg-Gly-Lys-Gly-Gly-Lys-Gly-Leu-Gly-Lys-Gly-Gly-Ala-Lys-Lys-His-Lys-Ala-Val
-
isozyme PAD3, at 37°C, 10 mM CaCl2, 50 mM NaCl, 100 mM Tris-HCl, pH 7.6, 2 mM dithiothreitol
0.5
Ac-Ser-Gly-Arg-Gly-Lys-Gly-Gly-Lys-Gly-Leu-Gly-Lys-Gly-Gly-Ala-Lys-Lys-His-Lys-Ala-Val
-
isozyme PAD4, at 37°C, 10 mM CaCl2, 50 mM NaCl, 100 mM Tris-HCl, pH 7.6, 2 mM dithiothreitol
1.2
Ac-Ser-Gly-Arg-Gly-Lys-Gly-Gly-Lys-Gly-Leu-Gly-Lys-Gly-Gly-Ala-Lys-Lys-His-Lys-Ala-Val
-
isozyme PAD1, at 37°C, 10 mM CaCl2, 50 mM NaCl, 100 mM Tris-HCl, pH 7.6, 2 mM dithiothreitol
0.7
histone H4-L-arginine
-
isozyme PAD3, at 37°C, 10 mM CaCl2, 50 mM NaCl, 100 mM Tris-HCl, pH 7.6, 2 mM dithiothreitol
4.3
histone H4-L-arginine
-
isozyme PAD1, at 37°C, 10 mM CaCl2, 50 mM NaCl, 100 mM Tris-HCl, pH 7.6, 2 mM dithiothreitol
9
histone H4-L-arginine
-
isozyme PAD4, at 37°C, 10 mM CaCl2, 50 mM NaCl, 100 mM Tris-HCl, pH 7.6, 2 mM dithiothreitol
0.184
IHAREIFDSR
pH 7.5, 37°C
0.391
IHAREIFDSR
pH 7.5, 37°C
0.13
N-alpha-benzoyl-L-arginine amide
-
isozyme PAD3, at 37°C, 10 mM CaCl2, 50 mM NaCl, 100 mM Tris-HCl, pH 7.6, 2 mM dithiothreitol
11
N-alpha-benzoyl-L-arginine amide
-
isozyme PAD4, at 37°C, 10 mM CaCl2, 50 mM NaCl, 100 mM Tris-HCl, pH 7.6, 2 mM dithiothreitol
22
N-alpha-benzoyl-L-arginine amide
-
isozyme PAD1, at 37°C, 10 mM CaCl2, 50 mM NaCl, 100 mM Tris-HCl, pH 7.6, 2 mM dithiothreitol
120000
N-alpha-benzoyl-L-arginine amide
-
mutant PAD3 enzyme G374R, at 37°C, 10 mM CaCl2, 50 mM NaCl, 100 mM Tris-HCl, pH 7.6, 2 mM dithiothreitol
0.005
N-alpha-benzoyl-L-arginine ethyl ester
pH 7.6, 37°C, mutant enzyme Q350A
0.007
N-alpha-benzoyl-L-arginine ethyl ester
mutant E412A, pH 7.6, 37°C
0.007
N-alpha-benzoyl-L-arginine ethyl ester
mutant R373A, pH 7.6, 37°C
0.007
N-alpha-benzoyl-L-arginine ethyl ester
pH 7.6, 37°C, mutant enzyme E412A
0.007
N-alpha-benzoyl-L-arginine ethyl ester
pH 7.6, 37°C, mutant enzyme W373A
0.02
N-alpha-benzoyl-L-arginine ethyl ester
mutant R372A, pH 7.5, 25°C
0.025
N-alpha-benzoyl-L-arginine ethyl ester
-
isozyme PAD3, at 37°C, 10 mM CaCl2, 50 mM NaCl, 100 mM Tris-HCl, pH 7.6, 2 mM dithiothreitol
0.05
N-alpha-benzoyl-L-arginine ethyl ester
mutant Q350A, pH 7.6, 37°C
0.05
N-alpha-benzoyl-L-arginine ethyl ester
mutant R372Q, pH 7.5, 25°C
0.075
N-alpha-benzoyl-L-arginine ethyl ester
mutant E352A, pH 7.6, 37°C
0.075
N-alpha-benzoyl-L-arginine ethyl ester
pH 7.6, 37°C, mutant enzyme E352A
0.1
N-alpha-benzoyl-L-arginine ethyl ester
mutant V469L, pH 7.5, 25°C
0.115
N-alpha-benzoyl-L-arginine ethyl ester
mutant D370A, pH 7.6, 37°C
0.115
N-alpha-benzoyl-L-arginine ethyl ester
pH 7.6, 37°C, mutant enzyme D370A
0.15
N-alpha-benzoyl-L-arginine ethyl ester
mutant V469A, pH 7.5, 25°C
0.195
N-alpha-benzoyl-L-arginine ethyl ester
mutant D374A, pH 7.6, 37°C
0.195
N-alpha-benzoyl-L-arginine ethyl ester
pH 7.6, 37°C, mutant enzyme D374A
0.35
N-alpha-benzoyl-L-arginine ethyl ester
mutant R347A, pH 7.6, 37°C
0.35
N-alpha-benzoyl-L-arginine ethyl ester
pH 7.6, 37°C, mutant enzyme R347
0.4
N-alpha-benzoyl-L-arginine ethyl ester
mutant V469T, pH 7.5, 25°C
0.845
N-alpha-benzoyl-L-arginine ethyl ester
mutant D389A, pH 7.6, 37°C
0.845
N-alpha-benzoyl-L-arginine ethyl ester
pH 7.6, 37°C, mutant enzyme D389A
0.9
N-alpha-benzoyl-L-arginine ethyl ester
mutant Y435A, pH 7.5, 25°C
1.065
N-alpha-benzoyl-L-arginine ethyl ester
mutant D166A, pH 7.6, 37°C
1.065
N-alpha-benzoyl-L-arginine ethyl ester
pH 7.6, 37°C, mutant enzyme D166A
1.1
N-alpha-benzoyl-L-arginine ethyl ester
mutant D177A, pH 7.6, 37°C
1.1
N-alpha-benzoyl-L-arginine ethyl ester
pH 7.6, 37°C, mutant enzyme D177A
1.4
N-alpha-benzoyl-L-arginine ethyl ester
mutant R441A, pH 7.5, 25°C
1.5
N-alpha-benzoyl-L-arginine ethyl ester
-
isozyme PAD1, at 37°C, 10 mM CaCl2, 50 mM NaCl, 100 mM Tris-HCl, pH 7.6, 2 mM dithiothreitol
1.9
N-alpha-benzoyl-L-arginine ethyl ester
mutant L279D, pH 7.5, 25°C
2
N-alpha-benzoyl-L-arginine ethyl ester
mutant W548K, pH 7.5, 25°C
3.2
N-alpha-benzoyl-L-arginine ethyl ester
mutant V284D, pH 7.5, 25°C
3.7
N-alpha-benzoyl-L-arginine ethyl ester
mutant V284A, pH 7.5, 25°C
4.4
N-alpha-benzoyl-L-arginine ethyl ester
-
isozyme PAD4, at 37°C, 10 mM CaCl2, 50 mM NaCl, 100 mM Tris-HCl, pH 7.6, 2 mM dithiothreitol
4.6
N-alpha-benzoyl-L-arginine ethyl ester
mutant W548F, pH 7.5, 25°C
6
N-alpha-benzoyl-L-arginine ethyl ester
mutant D125A, pH 7.6, 37°C
6
N-alpha-benzoyl-L-arginine ethyl ester
pH 7.6, 37°C, mutant enzyme D125A
6.3
N-alpha-benzoyl-L-arginine ethyl ester
mutant V283T, pH 7.5, 25°C
6.8
N-alpha-benzoyl-L-arginine ethyl ester
mutant D123N, pH 7.6, 37°C
6.8
N-alpha-benzoyl-L-arginine ethyl ester
pH 7.6, 37°C, mutant enzyme D123N
7.1
N-alpha-benzoyl-L-arginine ethyl ester
mutant R639A, pH 7.5, 25°C
7.9
N-alpha-benzoyl-L-arginine ethyl ester
mutant D169A, pH 7.6, 37°C
7.9
N-alpha-benzoyl-L-arginine ethyl ester
pH 7.6, 37°C, mutant enzyme D169A
8.6
N-alpha-benzoyl-L-arginine ethyl ester
mutant F285A, pH 7.5, 25°C
8.8
N-alpha-benzoyl-L-arginine ethyl ester
mutant D465A, pH 7.5, 25°C
9
N-alpha-benzoyl-L-arginine ethyl ester
mutant L6D, pH 7.5, 25°C
9.3
N-alpha-benzoyl-L-arginine ethyl ester
mutant R374Q, pH 7.5, 25°C
11
N-alpha-benzoyl-L-arginine ethyl ester
mutant F541A, pH 7.5, 25°C
11.1
N-alpha-benzoyl-L-arginine ethyl ester
mutant R374A, pH 7.5, 25°C
11.4
N-alpha-benzoyl-L-arginine ethyl ester
mutant V283A, pH 7.5, 25°C
12.2
N-alpha-benzoyl-L-arginine ethyl ester
mutant L6A, pH 7.5, 25°C
13.8
N-alpha-benzoyl-L-arginine ethyl ester
mutant L279A, pH 7.5, 25°C
16.5
N-alpha-benzoyl-L-arginine ethyl ester
pH 7.6, 37°C, wild-type enzyme
16.5
N-alpha-benzoyl-L-arginine ethyl ester
wild-type, pH 7.6, 37°C
18
N-alpha-benzoyl-L-arginine ethyl ester
mutant F221A/F222A, pH 7.6, 37°C
18
N-alpha-benzoyl-L-arginine ethyl ester
pH 7.6, 37°C, mutant enzyme F221A/F222A
19
N-alpha-benzoyl-L-arginine ethyl ester
mutant V283I, pH 7.5, 25°C
20
N-alpha-benzoyl-L-arginine ethyl ester
mutant C434A, pH 7.5, 25°C
21.8
N-alpha-benzoyl-L-arginine ethyl ester
mutant L6I, pH 7.5, 25°C
21.8
N-alpha-benzoyl-L-arginine ethyl ester
mutant V284I, pH 7.5, 25°C
23.4
N-alpha-benzoyl-L-arginine ethyl ester
wild-type, pH 7.5, 25°C
24.5
N-alpha-benzoyl-L-arginine ethyl ester
mutant F576A, pH 7.5, 25°C
28
N-alpha-benzoyl-L-arginine ethyl ester
mutant L279I, pH 7.5, 25°C
13800
N-alpha-benzoyl-L-arginine ethyl ester
-
mutant PAD3 enzyme G374R, at 37°C, 10 mM CaCl2, 50 mM NaCl, 100 mM Tris-HCl, pH 7.6, 2 mM dithiothreitol
0.12
N-alpha-benzoyl-L-arginine methyl ester
-
isozyme PAD3, at 37°C, 10 mM CaCl2, 50 mM NaCl, 100 mM Tris-HCl, pH 7.6, 2 mM dithiothreitol
3.3
N-alpha-benzoyl-L-arginine methyl ester
-
isozyme PAD4, at 37°C, 10 mM CaCl2, 50 mM NaCl, 100 mM Tris-HCl, pH 7.6, 2 mM dithiothreitol
10.4
N-alpha-benzoyl-L-arginine methyl ester
-
isozyme PAD1, at 37°C, 10 mM CaCl2, 50 mM NaCl, 100 mM Tris-HCl, pH 7.6, 2 mM dithiothreitol
0.63
Nalpha-benzoyl L-arginine ethyl ester
-
37°C, pH 7.6, mutant R419Q
1.3
Nalpha-benzoyl L-arginine ethyl ester
-
37°C, pH 7.6, mutant R639Q
1.5
Nalpha-benzoyl L-arginine ethyl ester
-
37°C, pH 7.6, mutant R156K
1.6
Nalpha-benzoyl L-arginine ethyl ester
-
37°C, pH 7.6, mutant R123Q
1.7
Nalpha-benzoyl L-arginine ethyl ester
-
37°C, pH 7.6, mutant R609K
1.8
Nalpha-benzoyl L-arginine ethyl ester
-
37°C, pH 7.6, mutant R374K
1.9
Nalpha-benzoyl L-arginine ethyl ester
-
37°C, pH 7.6, mutant A112G
1.9
Nalpha-benzoyl L-arginine ethyl ester
-
37°C, pH 7.6, mutant R374Q
2.1
Nalpha-benzoyl L-arginine ethyl ester
-
37°C, pH 7.6, mutant A82V
2.1
Nalpha-benzoyl L-arginine ethyl ester
-
37°C, pH 7.6, mutant R609Q
2.2
Nalpha-benzoyl L-arginine ethyl ester
-
37°C, pH 7.6, mutant R156Q
2.4
Nalpha-benzoyl L-arginine ethyl ester
-
37°C, pH 7.6, mutant R484K
2.5
Nalpha-benzoyl L-arginine ethyl ester
-
37°C, pH 7.6, mutant R205K
2.5
Nalpha-benzoyl L-arginine ethyl ester
-
37°C, pH 7.6, mutant R205Q
2.7
Nalpha-benzoyl L-arginine ethyl ester
-
37°C, pH 7.6, mutant R484Q
2.8
Nalpha-benzoyl L-arginine ethyl ester
-
37°C, pH 7.6, mutant R639K
4.3
Nalpha-benzoyl L-arginine ethyl ester
-
37°C, pH 7.6, mutant R123K
4.3
Nalpha-benzoyl L-arginine ethyl ester
-
37°C, pH 7.6, mutant R419K
4.8
Nalpha-benzoyl L-arginine ethyl ester
-
37°C, pH 7.6, wild-type
6.4
Nalpha-benzoyl L-arginine ethyl ester
-
37°C, pH 7.6, mutant S55G
0.255
PPGFSPFR
pH 7.5, 37°C
0.563
PPGFSPFR
pH 7.5, 37°C
0.0031
[histone H4]-L-Arg
-
37°C, pH 7.6, mutant R372K, value below
0.012
[histone H4]-L-Arg
-
37°C, pH 7.6, mutant R372Q, value below
2.1
[histone H4]-L-Arg
-
37°C, pH 7.6, mutant R205Q
3.7
[histone H4]-L-Arg
-
37°C, pH 7.6, mutant R205K
4
[histone H4]-L-Arg
-
37°C, pH 7.6, mutant A82V
4.9
[histone H4]-L-Arg
-
37°C, pH 7.6, mutant R609K
5.1
[histone H4]-L-Arg
-
37°C, pH 7.6, mutant R374Q
5.3
[histone H4]-L-Arg
-
37°C, pH 7.6, mutant R484Q
5.5
[histone H4]-L-Arg
-
37°C, pH 7.6, mutant R419Q
5.5
[histone H4]-L-Arg
-
37°C, pH 7.6, mutant S55G
5.6
[histone H4]-L-Arg
-
37°C, pH 7.6, mutant R374K
5.9
[histone H4]-L-Arg
-
37°C, pH 7.6, mutant A112G
6.2
[histone H4]-L-Arg
-
37°C, pH 7.6, mutant R484K
7.1
[histone H4]-L-Arg
-
37°C, pH 7.6, mutant R123K
7.2
[histone H4]-L-Arg
-
37°C, pH 7.6, mutant R156Q
7.8
[histone H4]-L-Arg
-
37°C, pH 7.6, mutant R156K
8.3
[histone H4]-L-Arg
-
37°C, pH 7.6, mutant R609Q
8.3
[histone H4]-L-Arg
-
37°C, pH 7.6, mutant R639Q
8.7
[histone H4]-L-Arg
-
37°C, pH 7.6, mutant R639K
9
[histone H4]-L-Arg
-
37°C, pH 7.6, mutant R123Q
9.3
[histone H4]-L-Arg
-
37°C, pH 7.6, mutant R419K
10
[histone H4]-L-Arg
-
37°C, pH 7.6, wild-type
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A112G
-
K0.5 for Ca2+ higher than wild-type, kcat/Km ([histone H4]-L-Arg) lower than wild-type, kcat/Km (Nalpha-benzoyl L-arginine ethyl ester) lower than wild-type
A82V
-
K0.5 for Ca2+ lower than wild-type, kcat/Km ([histone H4]-L-Arg) lower than wild-type, kcat/Km (Nalpha-benzoyl L-arginine ethyl ester) lower than wild-type
A82V/A112G
-
naturally occuring single-nucleotide polymorphisms and site-directed mutagenesis, the SNP-PADI4 mutant haplotype shows a higher risk of rheumatoid arthritis due to correlation with the RA gene, because the mutant shows increased activity, compared to the wild-type PADI4, which promotes the autoimmune disease and apoptosis, overview
C434A
small decrase in kcat/Km value
D273A/R544A
-
Km (Nalpha-benzoyl-L-arginine ethyl ester): 0.35 mM, kcat (Nalpha-benzoyl-L-arginine ethyl ester): 13.5/sec, quaternary structure: dimer, Kd: 0.68 microM
D350A
no enzymic activity, catalytic residue
D369A
no enzymic activity, ligand for Ca2 coordination
D388A
81% of wild type activity, ligand for Ca4 coordination
D465A
residue involved in dimerization, about 30% of wild-type catalytic effciency
D473A
no enzymic activity, catalytic residue
D547A
-
Km (Nalpha-benzoyl-L-arginine ethyl ester): 0.36 mM, kcat (Nalpha-benzoyl-L-arginine ethyl ester): 11.6/sec, quaternary structure: monomer/dimer, Kd: 6.4 microM
D547E
-
Km (Nalpha-benzoyl-L-arginine ethyl ester): 0.72 mM, kcat (Nalpha-benzoyl-L-arginine ethyl ester): 8.9/sec, quaternary structure: monomer/dimer, Kd: 11.2 microM
D547N
-
Km (Nalpha-benzoyl-L-arginine ethyl ester): 0.86 mM, kcat (Nalpha-benzoyl-L-arginine ethyl ester): 12.2/sec, quaternary structure: monomer/dimer, Kd: 4.9 microM
E281A
-
Km (Nalpha-benzoyl-L-arginine ethyl ester): 0.45 mM, kcat (Nalpha-benzoyl-L-arginine ethyl ester): 12.1/sec, quaternary structure: dimer, Kd: 0.1 microM
E351A
33% of wild type activity, ligand for Ca2 coordination
E353A
no enzymic activity, ligand for Ca1 coordination
E354A
2000fold decrease in activity
E411A
no enzymic activity, ligand for Ca1 coordination
F285AV284A
residue at dimer interface. Mutation decreases dimer formation and, consequently, enzyme activity
F541A
residue at dimer interface, about 50% decrease in kcat/Km value
F576A
residue at dimer interface, about 50% decrease in kcat/Km value
G374R
-
the PAD3 mutant does not display PAD4-like kinetics with benzoylated arginine derivatives
H471A
no enzymic activity, catalytic residue
L279A
residue at dimer interface. Mutation decreases dimer formation and, consequently, enzyme activity
L279D
residue at dimer interface. Mutation decreases dimer formation and, consequently, enzyme activity
L279I
residue at dimer interface. Mutation decreases dimer formation and, consequently, enzyme activity
L6A
residue at dimer interface. Mutation decreases dimer formation and, consequently, enzyme activity
L6D
residue at dimer interface. Mutation decreases dimer formation and, consequently, enzyme activity
L6I
residue at dimer interface. Mutation decreases dimer formation and, consequently, enzyme activity
N373A
no enzymic activity, ligand for Ca2 coordination
N648A
37% of wild type activity, ligand for water-mediated Ca1 coordination
Q349A
no enzymic activity, ligand for Ca1 coordination
R123K
-
K0.5 for Ca2+ lower than wild-type, kcat/Km ([histone H4]-L-Arg) lower than wild-type, kcat/Km (Nalpha-benzoyl L-arginine ethyl ester) comparable to wild-type
R123Q
-
K0.5 for Ca2+ higher than that obtained with the R123K mutant, but lower than wild-type, kcat/Km ([histone H4]-L-Arg) comparable to wild-type, kcat/Km (Nalpha-benzoyl L-arginine ethyl ester) comparable to wild-type
R156K
-
K0.5 for Ca2+ lower than wild-type, kcat/Km ([histone H4]-L-Arg) lower than wild-type, kcat/Km (Nalpha-benzoyl L-arginine ethyl ester) lower than wild-type
R156Q
-
K0.5 for Ca2+ comparable to wild-type, kcat/Km ([histone H4]-L-Arg) lower than wild-type, kcat/Km (Nalpha-benzoyl L-arginine ethyl ester) lower than wild-type
R205K
-
K0.5 for Ca2+ lower than wild-type, kcat/Km ([histone H4]-L-Arg) lower than wild-type, kcat/Km (Nalpha-benzoyl L-arginine ethyl ester) lower than wild-type
R205Q
-
K0.5 for Ca2+ lower than wild-type, kcat/Km ([histone H4]-L-Arg) lower than wild-type, kcat/Km (Nalpha-benzoyl L-arginine ethyl ester) lower than wild-type
R372A
almost complete loss of activity
R373A
2000fold decrease in activity
R374K
-
K0.5 for Ca2+ lower than wild-type, kcat/Km ([histone H4]-L-Arg) lower than wild-type, kcat/Km (Nalpha-benzoyl L-arginine ethyl ester) lower than wild-type
R374Q
-
K0.5 for Ca2+ comparable to wild-type, kcat/Km ([histone H4]-L-Arg) lower than wild-type, kcat/Km (Nalpha-benzoyl L-arginine ethyl ester) lower than wild-type
R419K
-
K0.5 for Ca2+ lower than wild-type, kcat/Km ([histone H4]-L-Arg) comparable to wild-type, kcat/Km (Nalpha-benzoyl L-arginine ethyl ester) comparable to wild-type
R419Q
-
K0.5 for Ca2+ lower than wild-type, kcat/Km ([histone H4]-L-Arg) lower than wild-type, kcat/Km (Nalpha-benzoyl L-arginine ethyl ester) lower than wild-type
R441A
residue involved in dimerization, about 6% of wild-type catalytic effciency
R484K
-
K0.5 for Ca2+ lower than wild-type, kcat/Km ([histone H4]-L-Arg) lower than wild-type, kcat/Km (Nalpha-benzoyl L-arginine ethyl ester) lower than wild-type
R484Q
-
K0.5 for Ca2+ lower than wild-type, kcat/Km ([histone H4]-L-Arg) lower than wild-type, kcat/Km (Nalpha-benzoyl L-arginine ethyl ester) lower than wild-type
R609K
-
K0.5 for Ca2+ comparable to wild-type, kcat/Km ([histone H4]-L-Arg) lower than wild-type, kcat/Km (Nalpha-benzoyl L-arginine ethyl ester) lower than wild-type
R609Q
-
K0.5 for Ca2+ comparable to wild-type, kcat/Km ([histone H4]-L-Arg) lcomparable to wild-type, kcat/Km (Nalpha-benzoyl L-arginine ethyl ester) lower than wild-type
R639A
about 30% decrease in kcat/Km value
R639K
-
K0.5 for Ca2+ lower than wild-type, kcat/Km ([histone H4]-L-Arg) comparable to wild-type, kcat/Km (Nalpha-benzoyl L-arginine ethyl ester) lower than wild-type
R639Q
-
K0.5 for Ca2+ lower than wild-type, kcat/Km ([histone H4]-L-Arg) comparable to wild-type, kcat/Km (Nalpha-benzoyl L-arginine ethyl ester) lower than wild-type
R8A
-
Km (Nalpha-benzoyl-L-arginine ethyl ester): 0.47 mM, kcat (Nalpha-benzoyl-L-arginine ethyl ester): 12.2/sec, quaternary structure: monomer/dimer, Kd: 9.3 microM
R8A/D547A
-
Km (Nalpha-benzoyl-L-arginine ethyl ester): 0.4 mM, kcat (Nalpha-benzoyl-L-arginine ethyl ester): 10.7/sec, quaternary structure: monomer/dimer, Kd: 3.9 microM
R8E
-
Km (Nalpha-benzoyl-L-arginine ethyl ester): 1.06 mM, kcat (Nalpha-benzoyl-L-arginine ethyl ester): 7.3/sec, quaternary structure: monomer, Kd: 45.6 microM
R8E/D547E
-
Km (Nalpha-benzoyl-L-arginine ethyl ester): 2.77 mM, kcat (Nalpha-benzoyl-L-arginine ethyl ester): 3.3/sec, quaternary structure: monomer, Kd: 24 microM
R8H
-
Km (Nalpha-benzoyl-L-arginine ethyl ester): 0.47 mM, kcat (Nalpha-benzoyl-L-arginine ethyl ester): 13.3/sec, quaternary structure: dimer, Kd: 0.47 microM
R8K
-
Km (Nalpha-benzoyl-L-arginine ethyl ester): 0.5 mM, kcat (Nalpha-benzoyl-L-arginine ethyl ester): 10.8/sec, quaternary structure: monomer/dimer, Kd: 10.2 microM
R8L
-
Km (Nalpha-benzoyl-L-arginine ethyl ester): 1.36 mM, kcat (Nalpha-benzoyl-L-arginine ethyl ester): 5.3/sec, quaternary structure: monomer, Kd: 16.8 microM
R8Q
-
Km (Nalpha-benzoyl-L-arginine ethyl ester): 0.6 mM, kcat (Nalpha-benzoyl-L-arginine ethyl ester): 12.2/sec, quaternary structure: monomer/dimer, Kd: 15.7 microM
S55G
-
K0.5 for Ca2+ lower than wild-type, kcat/Km ([histone H4]-L-Arg) lower than wild-type, kcat/Km (Nalpha-benzoyl L-arginine ethyl ester) higher than wild-type
S55G/A112G
-
naturally occuring single-nucleotide polymorphisms and site-directed mutagenesis, the SNP-PADI4 mutant haplotype shows a higher risk of rheumatoid arthritis due to correlation with the RA gene, because the mutant shows increased activity, compared to the wild-type PADI4, which promotes the autoimmune disease and apoptosis, overview
S55G/A82V
-
naturally occuring single-nucleotide polymorphisms and site-directed mutagenesis, the SNP-PADI4 mutant haplotype shows reduced activity compared to the wild-type PADI4
S55G/A82V/A112G
-
naturally occuring single-nucleotide polymorphisms and site-directed mutagenesis, the SNP-PADI4 mutant haplotype shows a higher risk of rheumatoid arthritis due to correlation with the RA gene, because the mutant shows increased activity, compared to the wild-type PADI4, which promotes the autoimmune disease and apoptosis, overview
V283A
residue at dimer interface. Mutation decreases dimer formation and, consequently, enzyme activity
V283D
residue at dimer interface. Mutation decreases dimer formation and, consequently, enzyme activity
V283I
residue at dimer interface. Mutation decreases dimer formation and, consequently, enzyme activity
V283T
residue at dimer interface. Mutation decreases dimer formation and, consequently, enzyme activity
V284A
residue at dimer interface. Mutation decreases dimer formation and, consequently, enzyme activity
V284D
residue at dimer interface. Mutation decreases dimer formation and, consequently, enzyme activity
V284I
residue at dimer interface. Mutation decreases dimer formation and, consequently, enzyme activity
V469A
residue is critical for substrate binding at the active site. Mutation leads to a severe reduction in the catalytic activity
V469L
residue is critical for substrate binding at the active site. Mutation leads to a severe reduction in the catalytic activity
V469T
residue is critical for substrate binding at the active site. Mutation leads to a severe reduction in the catalytic activity
W347A
residue is critical for substrate binding at the active site. Mutation leads to a severe reduction in the catalytic activity
W347F
residue is critical for substrate binding at the active site. Mutation leads to a severe reduction in the catalytic activity
W373A
50fold decrease in kcat/Km compared to wild-type value with N-alpha-benzoyl-L-arginine ethyl ester as substrate
W548A
residue at dimer interface, complete loss of activity
W548F
residue at dimer interface, about 50% decrease in kcat/Km value
W548K
residue at dimer interface, about 50% decrease in kcat/Km value
Y237A
-
Km (Nalpha-benzoyl-L-arginine ethyl ester): 0.36 mM, kcat (Nalpha-benzoyl-L-arginine ethyl ester): 13.9/sec, quaternary structure: dimer, Kd: 0.29 microM
Y237A/E281A
-
Km (Nalpha-benzoyl-L-arginine ethyl ester): 0.38 mM, kcat (Nalpha-benzoyl-L-arginine ethyl ester): 13.2/sec, quaternary structure: dimer, Kd: 0.1 microM
Y435N
-
Km (Nalpha-benzoyl-L-arginine ethyl ester): 2.73 mM, kcat (Nalpha-benzoyl-L-arginine ethyl ester): 4/sec, quaternary structure: monomer, Kd: 33.8 microM
DELTA1-385
-
IKKgamma co-immunoprecipitates truncation mutants, PAD2(1-385) and PAD2(355-672)
DELTA355-672
-
IKKgamma co-immunoprecipitates truncation mutants, PAD2(1-385) and PAD2(355-672)
Q358A
-
substitution of Gln-358 (a putative ligand for Ca2+ binding) with an Ala abolishes co-immunoprecipitation with IKKgamma
C351S
-
the mutant is catalytically inactive, exhibiting less than 0.01% wild type activity
R152A
significant reduction in activity
R154A
activity is similar to wild-type
R152A
-
significant reduction in activity
-
R154A
-
activity is similar to wild-type
-
C645A
inactive mutant
C645A
no enzymic activity, catalytic residue
C645A
site-directed mutagemesis, inactive mutant, substrate-bound structure analysis
D123N
2.5fold decrease in activity
D123N
2.5fold decrease in kcat/Km compared to wild-type value with N-alpha-benzoyl-L-arginine ethyl ester as substrate
D125A
2.75fold decrease in activity
D125A
2.75fold decrease in kcat/Km compared to wild-type value with N-alpha-benzoyl-L-arginine ethyl ester as substrate
D166A
15fold decrease in activity
D166A
85fold decrease in kcat/Km compared to wild-type value with N-alpha-benzoyl-L-arginine ethyl ester as substrate
D169A
2fold decrease in activity
D169A
2fold decrease in kcat/Km compared to wild-type value with N-alpha-benzoyl-L-arginine ethyl ester as substrate
D177A
15fold decrease in activity
D177A
15fold decrease in kcat/Km compared to wild-type value with N-alpha-benzoyl-L-arginine ethyl ester as substrate
D370A
145fold decrease in activity
D370A
220fold decrease in kcat/Km compared to wild-type value with N-alpha-benzoyl-L-arginine ethyl ester as substrate
D374A
145fold decrease in kcat/Km compared to wild-type value with N-alpha-benzoyl-L-arginine ethyl ester as substrate
D374A
85fold decrease in activity
D389A
20fold decrease in activity
D389A
20fold decrease in kcat/Km compared to wild-type value with N-alpha-benzoyl-L-arginine ethyl ester as substrate
E352A
1250fold decrease in kcat/Km compared to wild-type value with N-alpha-benzoyl-L-arginine ethyl ester as substrate
E352A
220fold decrease in activity
E412A
200fold decrease in activity
E412A
3300fold decrease in kcat/Km compared to wild-type value with N-alpha-benzoyl-L-arginine ethyl ester as substrate
F221A/F222A
1.1fold increase in kcat/Km compared to wild-type value with N-alpha-benzoyl-L-arginine ethyl ester as substrate
F221A/F222A
slight increase in activity, mutations do not significantly alter the calcium dependence of the enzyme
Q350A
2350fold decrease in kcat/Km compared to wild-type value with N-alpha-benzoyl-L-arginine ethyl ester as substrate
Q350A
3000fold decrease in activity
R347A
50fold decrease in activity
R347A
fold decrease in kcat/Km compared to wild-type value with N-alpha-benzoyl-L-arginine ethyl ester as substrate
R372K
complete loss of activity
R372K
-
830fold decreased in kcat/M with either substrate [histone H4]-L-Arg or Nalpha-benzoyl L-arginine ethyl ester
R372Q
almost complete loss of activity
R372Q
-
830fold decreased in kcat/M with either substrate [histone H4]-L-Arg or Nalpha-benzoyl L-arginine ethyl ester
Y435A
-
Km (Nalpha-benzoyl-L-arginine ethyl ester): 2.33 mM, kcat (Nalpha-benzoyl-L-arginine ethyl ester): 5.9/sec, quaternary structure: monomer, Kd: 30.3 microM
Y435A
residue involved in dimerization, about 4% of wild-type catalytic effciency
additional information
the promoter activity of MFZ1- and Sp1-binding site mutants is reduced, overview
additional information
-
the promoter activity of MFZ1- and Sp1-binding site mutants is reduced, overview
additional information
generation of PAD6-deficient mice, enzyme inactivation affects female, but not male, fertility, it results in dispersal of the cytoskeletal sheets in oocytes, phenotype, overview
additional information
inactivation of PAD2 does not affect PAD4 activity, but leads to limited deimination due to the narrower cleavage spectrum of arginyl residues in myelin basic protein for PAD4, overview
additional information
inactivation of PAD2 does not affect PAD4 activity, but leads to limited deimination due to the narrower cleavage spectrum of arginyl residues in myelin basic protein for PAD4, overview
additional information
-
inactivation of PAD2 does not affect PAD4 activity, but leads to limited deimination due to the narrower cleavage spectrum of arginyl residues in myelin basic protein for PAD4, overview
-
additional information
-
strain T2 shows three amino-acid substitutions directly preceding catalytic residue H236 (G231N/E232T/N235D) when compared with PPAD from the reference strain ATCC 33277. Mutations localize to a loop engaged in substrate/inhibitor binding. Mutation of these positions in the reference strain results in twofold higher cell-associated citrullinating activity. Similar to PPAD-T1, recombinant PPAD-T2 citrullinates arginines at the C-termini of general peptidic substrates but not within peptides
additional information
strain T2 shows three amino-acid substitutions directly preceding catalytic residue H236 (G231N/E232T/N235D) when compared with PPAD from the reference strain ATCC 33277. Mutations localize to a loop engaged in substrate/inhibitor binding. Mutation of these positions in the reference strain results in twofold higher cell-associated citrullinating activity. Similar to PPAD-T1, recombinant PPAD-T2 citrullinates arginines at the C-termini of general peptidic substrates but not within peptides
additional information
-
strain T2 shows three amino-acid substitutions directly preceding catalytic residue H236 (G231N/E232T/N235D) when compared with PPAD from the reference strain ATCC 33277. Mutations localize to a loop engaged in substrate/inhibitor binding. Mutation of these positions in the reference strain results in twofold higher cell-associated citrullinating activity. Similar to PPAD-T1, recombinant PPAD-T2 citrullinates arginines at the C-termini of general peptidic substrates but not within peptides
-
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