Reference on EC 3.5.2.3 - dihydroorotase
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REF. 
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Coleman, P.F.; Suttle, D.P.; Stark, G.R.
Purification of a multifunctional protein bearing carbamyl-phosphate synthase, aspartate transcarbamylase, and dihydroorotase enzyme activities from mutant hamster cells
Methods Enzymol.
51
121-134
1978
Cricetinae
Mori, M.; Tatibana, M.
A multienzyme complex of carbamoyl-phosphate synthase (glutamine):aspartate carbamoyltransferase:dihydroorotase (rat ascites hepatoma cells and rat liver)
Methods Enzymol.
51
111-121
1978
Rattus norvegicus
Christopherson, R.I.; Williams, N.K.; Schoettle, S.L.; Szabados, E.; Hambley, T.W.; Manthey, M.K.
Inhibitors of dihydroorotase, amidophosphoribosyltransferase and IMP cyclohydrolase as potential drugs
Biochem. Soc. Trans.
23
888-893
1995
Cricetinae
Mukherjee, T.; Ray, M.; Bhaduri, A.
Aspartate transcarbamylase from Leishmania donovani
J. Biol. Chem.
263
708-713
1988
Leishmania donovani
Carrey, E.A.; Hardie, D.G.
Mapping of catalytic domains and phosphorylation sites in the multifunctional pyrimidine-biosynthetic protein CAD
Eur. J. Biochem.
171
583-588
1988
Cricetinae
Kelly, R.E.; Mally, M.I.; Evans, D.R.
The dihydroorotase domain of the multifunctional protein CAD
J. Biol. Chem.
261
6073-6083
1986
Betapolyomavirus macacae
Washabough, M.W.; Collins, K.D.
Dihydroorotase from Escherichia colii. Sulfhydryl group-metal ion interactions
J. Biol. Chem.
261
5920-5929
1986
Escherichia coli
Pettigrew, D.W.; Mehta, B.J.; Bidigare, R.R.; Choudhuri, R.R.; Scheffler, J.F.; Sander, E.G.
Enzyme elements involved in the interconversion of L-carbamylaspartate and L-dihydroorotate by dihydroorotase from Clostridium oroticum
Arch. Biochem. Biophys.
243
447-553
1985
Faecalicatena orotica
Bidigare, R.R.; Sander, E.G.; Pettigrew, D.W.
Evidence for a pH-dependent isomerization of Clostridium oroticum dihydroorotase
Biochim. Biophys. Acta
831
159-160
1985
Faecalicatena orotica
Pettigrew, D.W.; Bidigare, R.R.; Mehta, B.J.; Williams, M.I.; Sander, E.G.
Dihydro-orotase from Clostridium oroticum
Biochem. J.
230
101-108
1985
Faecalicatena orotica
Washabaugh, M.W.; Collins, K.D.
Dihydroorotase from Escherichia coli. Purification and characterization
J. Biol. Chem.
259
3293-3298
1984
Escherichia coli
Davidson, J.N.; Rumsby, P.C.; Tamaren, J.
Organization of a multifunctional protein in pyrimidine biosynthesis
J. Biol. Chem.
256
5220-5225
1981
Cricetinae
Hammond, D.J.; Gutteridge W.E.
Enzymes of pyrimidine biosynthesis in Trypanosoma cruzi
FEBS Lett.
118
259-262
1980
Trypanosoma cruzi
Scheffler, J.E.; MA, J.; Sander E.G.
Dihydroorotase from Clostridium oroticum is an allosteric enzyme
Biochem. Biophys. Res. Commun.
91
563-568
1979
Faecalicatena orotica
Christopherson, R.I.; Jones, M.E.
Interconvension of carbamyl-L-aspartate and L-dihydroorotate by dihydroorotase from mouse Ehrlich ascites carcinoma
J. Biol. Chem.
254
12506-12512
1979
Mus musculus
Taylor, W.H.; Taylor, M.L.; Balch, W.E.; Gilchrist, P.S.
Purification and properties of dihydroorotase, a zinc-containing metalloenzyme in Clostridium oroticum
J. Bacteriol.
127
863-873
1976
Faecalicatena orotica
Kennedy, J.
Dihydroorotase from rat liver: purification, properties and regulatory role in pyrimidine biosynthesis
Arch. Biochem. Biophys.
160
358-365
1974
Rattus norvegicus
Serre, V.; Guy, H.; Liu, X.; Penverne, B.; Herve, G.; Evans, D.
Allosteric regulation and substrate channeling in multifunctional pyrimidine biosynthetic complexes: analysis of isolated domains and yeast-mammalian chimeric proteins
J. Mol. Biol.
281
363-377
1998
Saccharomyces cerevisiae, Cricetinae
Krungkrai, J.; Cerami, A.; Henderson, G.B.
Pyrimidine biosynthesis in parasitic protozoa: purification of a monofunctional dihydroorotase from Plasmodium berghei and Crithidia fasciculata
Biochemistry
29
6270-6275
1990
Crithidia fasciculata, Plasmodium berghei
Krungkrai, J.; Krungkrai, S.R.; Phakanont, K.
Antimalarial activity of orotate analogs that inhibit dihydroorotase and dihydroorotate dehydrogenase
Biochem. Pharmacol.
43
1295-1301
1992
Plasmodium berghei
Williams, N.K.; Manthey, M.K.; Hambley, T.W.; O'Donoghue, S.I.; Keegan, M.; Chapman, B.E.; Christopherson, R.I.
Catalysis by hamster dihydroorotase: zinc binding, site-directed mutagenesis, and interaction with inhibitors
Biochemistry
34
11344-11352
1995
Cricetinae
Ogawa, J.; Shimizu, S.
Purification and characterization of dihydroorotase from Pseudomonas putida
Arch. Microbiol.
164
353-357
1995
Pseudomonas putida
Williams, N.K.; Peide, Y.; Seymour, K.K.; Ralston, G.B.; Christopherson, R.I.
Expression of catallytically active hamster dihydroorotase domain in Escherichia coli: purification and characterization
Protein Eng.
6
333-340
1993
Cricetinae
Aleksenko.A.; Liu, W.; Gojkovic, Z.; Nielsen, J.; Piskur, J.
Structural and transcriptional analysis of the pyrABCN, pyrD and pyrF genes in Aspergillus nidulans and the evolutionary origin of fungal dihydroorotases
Mol. Microbiol.
33
599-611
1999
Aspergillus nidulans
Purcarea, C.; Martin, P.; Vickrey, J.F.; Guy, H.I.; Edwards, B.F.; Evans, D.R.
Cloning, expression and preliminary X-ray analysis of the dihydroorotase from the hyperthermophilic eubacterium Aquifex aeolicus
Acta Crystallogr. Sect. D
58
154-156
2002
Aquifex aeolicus
Maher, M.J.; Huang, D.T.; Guss, J.M.; Collyer, C.A.; Christopherson, R.I.
Crystallization of hamster dihydroorotase: involvement of a disulfide-linked tetrameric form
Acta Crystallogr. Sect. D
59
381-384
2003
Mesocricetus auratus
Thoden, J.B.; Phillips, G.N., Jr.; Neal, T.M.; Raushel, F.M.; Holden, H.M.
Molecular structure of dihydroorotase: a paradigm for catalysis through the use of a binuclear metal center
Biochemistry
40
6989-6997
2001
Escherichia coli
Schroeder, P.E.; Davidson, J.N.; Hasinoff, B.B.
Dihydroorotase catalyzes the ring opening of the hydrolysis intermediates of the cardioprotective drug dexrazoxane (ICRF-187)
Drug Metab. Dispos.
30
1431-1435
2002
Mesocricetus auratus
Ahuja, A.; Purcarea, C.; Ebert, R.; Sadecki, S.; Guy, H.I.; Evans, D.R.
Aquifex aeolicus dihydroorotase: association with aspartate transcarbamoylase switches on catalytic activity
J. Biol. Chem.
279
53136-53144
2004
Aquifex aeolicus
Brichta, D.M.; Azad, K.N.; Ralli, P.; O'Donovan, G.A.
Pseudomonas aeruginosa dihydroorotases: a tale of three pyrCs
Arch. Microbiol.
182
7-17
2004
Pseudomonas aeruginosa (P72170), Pseudomonas aeruginosa
McPhail, D.; Shepherdson, M.
The aspartate transcarbamoylase-dihydroorotase complex in Deinococcus radiophilus has an active dihydroorotase
Arch. Microbiol.
185
78-81
2006
Deinococcus radiophilus, Deinococcus radiophilus NCIMB 10648
Porter, T.N.; Li, Y.; Raushel, F.M.
Mechanism of the dihydroorotase reaction
Biochemistry
43
16285-16292
2004
Escherichia coli (P05020)
Anderson, M.A.; Cleland, W.W.; Huang, D.T.; Chan, C.; Shojaei, M.; Christopherson, R.I.
13C and 15N isotope effects for conversion of L-dihydroorotate to N-carbamyl-L-aspartate using dihydroorotase from hamster and Bacillus caldolyticus
Biochemistry
45
7132-7139
2006
[Bacillus] caldolyticus, Mesocricetus auratus
Huang, D.T.; Kaplan, J.; Menz, R.I.; Katis, V.L.; Wake, R.G.; Zhao, F.; Wolfenden, R.; Christopherson, R.I.
Thermodynamic analysis of catalysis by the dihydroorotases from hamster and Bacillus caldolyticus, as compared with the uncatalyzed reaction
Biochemistry
45
8275-8283
2006
[Bacillus] caldolyticus, Cricetinae, Mesocricetus auratus
Li, Y.; Raushel, F.M.
Inhibitors designed for the active site of dihydroorotase
Bioorg. Chem.
33
470-483
2005
Escherichia coli
Lee, M.; Chan, C.W.; Mitchell Guss, J.; Christopherson, R.I.; Maher, M.J.
Dihydroorotase from Escherichia coli: loop movement and cooperativity between subunits
J. Mol. Biol.
348
523-533
2005
Arabidopsis thaliana (O04904), Escherichia coli (P05020), Escherichia coli, Helicobacter pylori (P56465), Homo sapiens (P27708), Mesocricetus auratus (P08955), Plasmodium falciparum (Q8IKA9), Saccharomyces cerevisiae (P20051)
Martin P.D; Purcarea, C.; Zhang, P.; Vaishnav, A.; Sadecki, S.; Guy-Evans, H.I.; Evans, D.R.; Edwards, B.F.
The crystal structure of a novel, latent dihydroorotase from Aquifex aeolicus at 1.7A resolution
J. Mol. Biol.
348
535-547
2005
Aquifex aeolicus (O66990), Aquifex aeolicus
Robles Lopez, S.M.; Hortua Triana, M.A.; Zimmermann, B.H.
Cloning and preliminary characterization of the dihydroorotase from Toxoplasma gondii
Mol. Biochem. Parasitol.
148
93-98
2006
Saccharomyces cerevisiae, Mycobacterium leprae, Plasmodium berghei (A0A509AX79), Arabidopsis thaliana (O04904), Trypanosoma cruzi (O76138), Escherichia coli (P05020), Mesocricetus auratus (P08955), Homo sapiens (P27708), Ustilago maydis (P31301), Toxoplasma gondii (Q8MXA5), Toxoplasma gondii
Chen, K.F.; Lai, Y.Y.; Sun, H.S.; Tsai, S.J.
Transcriptional repression of human cad gene by hypoxia inducible factor-1alpha
Nucleic Acids Res.
33
5190-5198
2005
Homo sapiens
Liang, Y.H.; Liu, X.; Wang, J.; Li, L.; Su, X.D.
Protein preperation, crystallization and preliminary X-ray crystallographic studies of dihydroorotase from Bacillus subtilis
Protein Pept. Lett.
12
717-719
2005
Bacillus subtilis
Lee, M.; Maher, M.J.; Guss, J.M.
Structure of the T109S mutant of Escherichia coli dihydroorotase complexed with the inhibitor 5-fluoroorotate: catalytic activity is reflected by the crystal form
Acta Crystallogr. Sect. F
F63
154-161
2007
Escherichia coli (P05020), Escherichia coli
Krungkrai, S.R.; Wutipraditkul, N.; Krungkrai, J.
Dihydroorotase of human malarial parasite Plasmodium falciparum differs from host enzyme
Biochem. Biophys. Res. Commun.
366
821-826
2008
Plasmodium falciparum
Lee, M.; Maher, M.J.; Christopherson, R.I.; Guss, J.M.
Kinetic and structural analysis of mutant Escherichia coli dihydroorotases: a flexible loop stabilizes the transition state
Biochemistry
46
10538-10550
2007
Escherichia coli (P05020), Escherichia coli
Lee, M.; Chan, C.W.; Graham, S.C.; Christopherson, R.I.; Guss, J.M.; Maher, M.J.
Structures of ligand-free and inhibitor complexes of dihydroorotase from Escherichia coli: implications for loop movement in inhibitor design
J. Mol. Biol.
370
812-825
2007
Escherichia coli (P05020), Escherichia coli
Zhang, P.; Martin, P.D.; Purcarea, C.; Vaishnav, A.; Brunzelle, J.S.; Fernando, R.; Guy-Evans, H.I.; Evans, D.R.; Edwards, B.F.
Dihydroorotase from the hyperthermophile Aquifex aeolicus is activated by stoichiometric association with aspartate transcarbamoylase and forms a one-pot reactor for pyrimidine biosynthesis
Biochemistry
48
766-778
2009
Aquifex aeolicus (O66990), Aquifex aeolicus
Schroeder, P.E.; Patel, D.; Hasinoff, B.B.
The dihydroorotase inhibitor 5-aminoorotic acid inhibits the metabolism in the rat of the cardioprotective drug dexrazoxane and its one-ring open metabolites
Drug Metab. Dispos.
36
1780-1785
2008
Rattus norvegicus
Adama, C.; Bernard, S.; Brice, B.; Felix, Y.; Joseph, D.
Optimization of biomass and dihydroorotase (DHOase) production by Saccharomyces cerevisiae MNJ3 (pMNJ1)
Afr. J. Biotechnol.
8
037-041
2009
Saccharomyces cerevisiae
-
West, T.P.
Effect of carbon source on pyrimidine biosynthesis in Pseudomonas oryzihabitans
J. Basic Microbiol.
50
397-400
2010
Pseudomonas oryzihabitans
Wang, C.C.; Tsau, H.W.; Chen, W.T.; Huang, C.Y.
Identification and characterization of a putative dihydroorotase, KPN01074, from Klebsiella pneumoniae
Protein J.
29
445-452
2010
Klebsiella pneumoniae (A6T7D6), Klebsiella pneumoniae
Mehboob, S.; Mulhearn, D.C.; Truong, K.; Johnson, M.E.; Santarsiero, B.D.
Structure of dihydroorotase from Bacillus anthracis at 2.6 A resolution
Acta Crystallogr. Sect. F
66
1432-1435
2010
Bacillus anthracis (Q81WF0), Bacillus anthracis
Nara, T.; Hashimoto, M.; Hirawake, H.; Liao, C.W.; Fukai, Y.; Suzuki, S.; Tsubouchi, A.; Morales, J.; Takamiya, S.; Fujimura, T.; Taka, H.; Mineki, R.; Fan, C.K.; Inaoka, D.K.; Inoue, M.; Tanaka, A.; Harada, S.; Kita, K.; Aoki, T.
Molecular interaction of the first 3 enzymes of the de novo pyrimidine biosynthetic pathway of Trypanosoma cruzi
Biochem. Biophys. Res. Commun.
418
140-143
2012
Trypanosoma cruzi (O76138), Trypanosoma cruzi
Peng, W.F.; Huang, C.Y.
Allantoinase and dihydroorotase binding and inhibition by flavonols and the substrates of cyclic amidohydrolases
Biochimie
101
113-122
2014
Klebsiella pneumoniae
Truong, L.; Hevener, K.E.; Rice, A.J.; Patel, K.; Johnson, M.E.; Lee, H.
High-level expression, purification, and characterization of Staphylococcus aureus dihydroorotase (PyrC) as a cleavable His-SUMO fusion
Protein Expr. Purif.
88
98-106
2013
Staphylococcus aureus (P65907), Staphylococcus aureus, Staphylococcus aureus MW2 (P65907)
Cheng, J.H.; Huang, Y.H.; Lin, J.J.; Huang, C.Y.
Crystal structures of monometallic dihydropyrimidinase and the human dihydroorotase domain K1556A mutant reveal no lysine carbamylation within the active site
Biochem. Biophys. Res. Commun.
505
439-444
2018
Homo sapiens (P27708), Homo sapiens
Tiwari, K.; Kumar, R.; Dubey, V.K.
Biochemical characterization of dihydroorotase of Leishmania donovani understanding pyrimidine metabolism through its inhibition
Biochimie
131
45-53
2016
Leishmania donovani (H9D0Z2), Leishmania donovani, Leishmania donovani BHU 1081 (H9D0Z2)
Rice, A.J.; Lei, H.; Santarsiero, B.D.; Lee, H.; Johnson, M.E.
Ca-asp bound X-ray structure and inhibition of Bacillus anthracis dihydroorotase (DHOase)
Bioorg. Med. Chem.
24
4536-4543
2016
Bacillus anthracis (Q81WF0), Bacillus anthracis, Bacillus anthracis Sterne (Q81WF0)
Prange, T.; Girard, E.; Fourme, R.; Dhaussy, A.C.; Edwards, B.; Vaishnav, A.; Patel, C.; Guy-Evans, H.; Herve, G.; Evans, D.R.
Pressure-induced activation of latent dihydroorotase from Aquifex aeolicus as revealed by high pressure protein crystallography
FEBS J.
286
1204-1213
2019
Aquifex aeolicus (O66990), Aquifex aeolicus
Lipowska, J.; Miks, C.D.; Kwon, K.; Shuvalova, L.; Zheng, H.; Lewinski, K.; Cooper, D.R.; Shabalin, I.G.; Minor, W.
Pyrimidine biosynthesis in pathogens - structures and analysis of dihydroorotases from Yersinia pestis and Vibrio cholerae
Int. J. Biol. Macromol.
136
1176-1187
2019
Yersinia pestis (Q8ZFU4), Yersinia pestis, Vibrio cholerae serotype O1 (Q9KL24), Vibrio cholerae serotype O1 El Tor Inaba N16961 (Q9KL24), Vibrio cholerae serotype O1 ATCC 39315 (Q9KL24)
Herve, G.; Evans, H.G.; Fernado, R.; Patel, C.; Hachem, F.; Evans, D.R.
Activation of latent dihydroorotase from Aquifex aeolicus by pressure
J. Biol. Chem.
292
629-637
2017
Aquifex aeolicus (O66990), Aquifex aeolicus
Del Cano-Ochoa, F.; Grande-Garcia, A.; Reverte-Lopez, M.; DAbramo, M.; Ramon-Maiques, S.
Characterization of the catalytic flexible loop in the dihydroorotase domain of the human multi-enzymatic protein CAD
J. Biol. Chem.
293
18903-18913
2018
Homo sapiens (P27708), Homo sapiens
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